2 '-Deoxyguanosine, 2 '-deoxycytidine, and 2 '-deoxyadenosine adducts resulting from the reaction of tetrahydrofuran with DNA bases

A recent study showed that tetrahydrofuran (THF), a widely used solvent, is carcinogenic in experimental animals. Despite its carcinogenic activity, there is a paucity of information regarding cellular toxicity, biomolecular damage, and genotoxicity induced by THF. We describe here the structural characterization of adducts produced by the reaction of oxidized THF with 2'-deoxyguanosine (dGuo-THF 1 and dGuo-THF 2), 2'-deoxyadenosine (dAdo-THF), and 2'-deoxycytidine (dCyd-THF). Adducts were isolated from in vitro reactions by reverse-phase HPLC and fully characterized on the basis of spectroscopic measurements. The stable derivatives obtained by the reduction of adducts with NaBH4 ( the case of dGuo-THF 1, dCyd-THF, and dAdo-THF) and the stable adduct dGuo-THF 2 were used as standards for optimization of chromatographic separations for adduct detection in DNA through HPLC/ESI/MSMS. Using this methodology, we successfully detected the four adducts in calf thymus DNA reacted with oxidized THF. The present study also provides evidence that rat liver microsomal monooxigenases oxidize THF to the reactive electrophilic compounds that are able to damage the DNA molecule, as indicated by a significant increase in adduct dGuo-THF 1 level when NADPH was added to the THF/ microsomes/dGuo incubation mixtures. Our data point to DNA-THF adducts as possible contributing factors to the toxicological effects of THF exposure.

A NEURAL NET FOR EXTRACTING KNOWLEDGE FROM NATURAL-LANGUAGE DATA-BASES

The present paper introduces a new model of fuzzy neuron, one which increases the computational power of the artificial neuron, turning it also into a symbolic processing device. This model proposes the synapsis to be symbolically and numerically defined, by means of the assignment of tokens to the presynaptic and postsynaptic neurons. The matching or concatenation compatibility between these tokens is used to decided about the possible connections among neurons of a given net. The strength of the compatible synapsis is made dependent on the amount of the available presynaptic and post synaptic tokens. The symbolic and numeric processing capacity of the new fuzzy neuron is used here to build a neural net (JARGON) to disclose the existing knowledge in natural language data bases such as medical files, set of interviews, and reports about engineering operations.

Variabilidade genética e filogeografia de populações de Prochilodus lineatus (Pisces: prochilodontidae) das bacias dos rios Paraguai-Paraná e da bacia Amazônica: bases para programas de conservação e de psicultura

A espécie Prochilodus lineatus, considerada um migrador por excelência, possui intensa ocorrência durante a migração reprodutiva. São peixes de elevado valor econômico e a sua adaptação em cativeiro os tornam altamente interessantes para desenvolvimento de programas de piscicultura. O monitoramento regular das variações genéticas nos estoques é importante em programas de conservação, evitando o declínio da variabilidade genética, essencial para a conservação das espécies. No entanto, os poucos dados moleculares populacionais para Prochilodus lineatus justificam a presente proposta. Nesse sentido, tivemos como objetivo caracterizar a variabilidade genética e estabelecer as relações filogeográficas entre 6 populações de P. lineatus das bacias dos rios Paraguai (2 do rio Paraguai e 3 do rio Cuiabá, MT) e Paraná (1 do rio Mogi-Guaçu, SP), num total de 34 indivíduos. Outros 16 indivíduos dos rios: da Prata, Uruguai, Paraná, Bermejo, Paraguai, Amazonas e Madeira, cujas sequências foram obtidas do National Center for Biotechnology Information (Genbank), foram analisados, totalizando assim, 50 indivíduos. Como grupo externo foi utilizado Salminus brasiliensis. O estudo foi realizado através das análises das sequências do gene mitocondrial ATPase 8/6. O interesse do uso de um gene mitocondrial está na vasta literatura que esse possui em análises filogenéticas e na confiança que existe nos dados gerados de tais análises. O gene citado foi completamente sequenciado (842pb), as análises filogenéticas foram conduzidas pelos métodos “Neighbor Joining (NJ)”, “Minimum Evolution (ME)” e “Máxima Parcimônia (MP)”, com 1000 réplicas de bootstrap no programa MEGA 5.0. Para análises filogeográficas as sequências foram analisadas no programa TCS e no programa Arlequin. Os resultados auxiliarão em uma melhor compreensão da história evolutiva, migração... (Resumo completo, clicar acesso eletrônico abaixo)

Bases Científicas da Acupuntura

Acupuncture is a technique belonging to traditional Chinese medicine that has existed for over 3000 years. It consists of stimuli by pressure, needling, heat and electrical stimulation of the skin at specific points to restore the health of patients. This technique has been increasingly incorporated into the West and therefore the research explaining its various mechanisms become very important to translate these ancient skills. This article is a literature review summarizing the basic concepts, historical and local, anti-inflammatory and neurological effects acupuncture in the body

Bases para criação de um Centro de Voluntariado na UNESP - Guaratinguetá

Volunteering is seen as a form of citizen participation enabling to involve, mobilize, engage and articulate people and institutions around issues of collective interest. Both for the volunteer and for the community involved, voluntary action can promote learning, new experiences and interaction with people previously unknown, as well as create opportunities for professional and personal growth. (CENTRO DE VOLUNTARIADO DE SÃO PAULO, 2012). This work starts from a survey conducted in 2006 by Carvalho, who detected the interest of members of the campus community Guaratinguetá UNESP in performing volunteer work. Its objective is to continue the work mentioned and think of ways to support volunteering in the Guaratinguetá Engineering College, mainly through the creation of connections between stakeholders (individuals and institutions). For this, focused Volunteer Centers and their functional models. Such a center could be the ideal facilitator of relationships between volunteer candidates that integrate FEG / UNESP community and institutions interested in the collaboration of these people. The work highlighted the benefits that voluntary action can bring to those involved in it, and the role that a Volunteer Center at UNESP Guaratinguetá may play, in the promotion and expansion of social activities already undertaken. The study also indicates the need for a validation of the proposals presented here and later detailing way of acting of the Centre in its institutional placement and maintenance costs

Proposta para a implantação de uma trilha interpretativa com bases na Pedagogia Waldorf na Floresta Estadual Edmundo Navarro de Andrade - Rio Claro, SP

At a time when people turn increasingly to technological innovations and virtual networks, a closer interaction with the environment can provide sensitizing experiences. One tool that facilitates this approach is the interpretative trail: its use in a Unit of Conservation (UC) allows more contact between the visitor and the nature elements. This research aimed to propose the establishment of an interpretative trail at the State Florest “Edmundo Navarro de Andrade” (FEENA) in Rio Claro (SP) with theoretical bases from Waldorf Pedagogy. The proposed trail is destined for students with 07-14 years-old, a period that the awareness is more accurate in a person, according to Waldorf tenets. On the path of the trail, we propose some educational activities at the points of interpretation: storytelling about environment; “photograph” the place with a drawing; create a song with sounds produced by the body and by nature elements; blindfold guide and a circle of people to share experiences. The activities aim to provide in the student a close and harmonious contact with oneself, with other people and with the natural world, and also serve as a support for programs of public use and environmental education at UC. The environmental awareness process in the context of FEENA - which has a significant landscape valuation - develops through the sensibilities of exterior and interior world. And also develops with the creation if images, thoughts, feelings and interdependencies relationships. Therefore, the ecologic education allows changes and formation of values and sensitive attitudes to students about the environment

Dinâmica folicular ovariana e bases para a aplicação das biotécnicas da reprodução em bovinos

In the last years Brazil has achieved a leading position in worldwide cattle breeding. This country is consolidating the practice of an intensive and technical level cattle breeding, due to greater emphasis on reproductive efficiency and genetic improvement. The attention given to reproductive efficiency became to enhance the genetic improvement process, by exploiting the full reproductive potential of the animals. The biotechniques applied to reproduction arised to expand the reproductive potential for greater economic return. Are the most important reproductive biotechniques the FTAI and in vivo and in vitro embryo production for embryo transfer. Different hormonal treatments are consolidating to interfere with normal physiology in order to achieve the desired goals. The FTAI works to increase the reproductive rates of cattle through pre-booked artificial insemination in all the females in reproduction, without the need for estrous detection. The in vivo and in vitro embryo production aims to distribute the genetics of males and females genetically improved by transferring their embryos to genetically inferior females. Superovulation treatments designed to prevent the mechanism of dominance, which results in many follicles ovulating simultaneously for in vivo embryo production. To the in vitro embryo production, the technique of ovarian puncture guided by transvaginal ultrasound allows to aspirate a number of quality oocytes. The application of biotech is based on knowledge of the female and ovarian reproductive physiology and knowledge of their acting in different breeds and environments.

Instrumentos econômicos na política ambiental: bases teóricas e aplicação prática

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Comportamento das curvas de saturação da água e LNAPL em solo da formação Rio Claro

Pós-graduação em Geociências e Meio Ambiente - IGCE

Bases teórico-metodológicas para a elaboração de um dicionário português-espanhol de economia

The aim of this article is to present the theoretic-methodological bases for the construction of a bilingual terminological dictionary of economics which serves either Portuguese and Spanish speakers. Th e contrasted languages are the Brazilian Portuguese variety and the peninsular and Argentinean varieties. The purpose of this text is based on the principles of the Communicative Theory of Terminology - CTT (Cabré, 1999).

Estudo de nanocompósitos luminescentes baseados em complexos de Eu(III) e Ir(III) ancorados à nanopartículas de sílica por bases de Schiff

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Reflexões sobre a variação terminológica na lexicografia corrente no Brasil e a construção das bases teórico-metodológicas para o dicionário de lexicografia brasileira

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Exometaboloma do tegumento da vespa social Polybia paulista por análises GC-MS: bases moleculares do reconhecimento de tipos parentais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bases biológicas para utilização de Trichospilus diatraeae (Hymenoptera: Eulophidae) para controle de Helicoverpa armigera (Lepidoptera: Noctuidae)

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Evaluation of the vitality of nelore calves born of normal or dystocic parturitions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)