Genetic transformation in Kappaphycus alvarezii using micro-particle bombardment: a potential strategy for germplasm improvement

This study investigated the delivery of a SV40 promoter driving lacZ gene into cells of Kappaphycus alvarezii using particle bombardment. Thallus pieces 0.5-0.8 mm in diameter and 1 cm in length were prepared as gene recipients. Bombardment parameters of 450 psi (rupture pressures) x 6 cm (particle travel distances), 650 psi x 6 cm, 1,100 psi x 6 cm and 1,100 psi x 9 cm were used. A significant increase in transformation efficiency from about 33% under the rupture pressure of 450 psi to 87% at 650 psi was observed in transformed thalli. Most of the positive cells appeared in epidermal cells bombarded at 450 psi, whereas positive signals were seen in both epidermal and medullary cells at 650 psi. No positive transient expression was detected at a bombardment of 1,100 psi, or in negative or blank controls. For the conditions tested, the best parameter was obtained at 650 psi at a distance of 6 cm. Thus, the strategy of taking vegetative thalli as recipients, using particle bombardment, and combining this with micro-propagation, together with developing an in vivo selectable marker, is a viable way to produce stable transformants, to eliminate chimeric expression, and to achieve transgenic breeding in K. alvarezii.

Genetic mapping of laminaria japonica and l. longissima using amplified fragment length polymorphism markers in a "two-way pseudo-testcross" strategy

With a "two-way pseudo-testcross" mapping strategy, we applied the amplified fragment length polymorphism (AFLP) markers to construct two moderate density genetic linkage maps for Laminaria. The linkage maps were generated from the 60 progenies of the F, cross family (Laminaria longissima Aresch. x L. japonica Miyabe) with twenty pairs of primer combinations. Of the 333 polymorphic loci scored in 60 progenies, 173 segregated in a 1:1 ratio, corresponding to DNA polymorphisms heterozygous in a single parent, and the other 58 loci existing in both parents followed a 3:1 Mendelian segregation ratio. Among the loci with 1:1 segregating ratios, 79 loci were ordered in 14 linkage groups (648.6 cM) of the paternal map, and 72 loci were ordered in 14 linkage groups (601.9 cM) of the maternal map. The average density of loci was approximately 1 per 8 cM. To investigate the homologies between two parental maps, we used 58 loci segregated 3:1 for further analysis, and deduced one homologous linkage group. The linkage data developed in these maps will be useful for detecting loci-controlling commercially important traits for Laminaria.

叠前地震数据规则化、重建及噪音压制

I address of reconstruction of spatial irregular sampling seismic data to regular grids. Spatial irregular sampling data impairs results of prestack migration, multiple attenuations, spectra estimation. Prestack 5-D volumes are often divided into sub-sections for further processing. Shot gathers are easy to obtain from irregular sampling volumes. My strategy for reconstruction is as follows: I resort irregular sampling gathers into a form of easy to bin and perform bin regularization, then utilize F-K inversion to reconstruct seismic data. In consideration of poor ability of F-K regularization to fill in large gaps, I sort regular sampling gathers to CMP and proposed high-resolution parabolic Radon transform to interpolate data and extrapolate offsets. To strong interfering noise--multiples, I use hybrid-domain high-resolution parabolic Radon transform to attenuate it. F-K regularization demand ultimately for lower computing costs. I proposed several methods to further improve efficiency of F-K inversion: first I introduce 1D and 2D NFFT algorithm for a rapid calculation of DFT operators; then develop fast 1D and 2D CG method to solve least-square equations, and utilize preconditioner to accelerate convergence of CG iterations; what’s more, I use Delaunay triangulation for weight calculation and use bandlimit frequency and varying bandwidth technique for competitive computation. Numerical 2D and 3D examples are offered to verify reasonable results and more efficiency. F-K regularization has poor ability to fill in large gaps, so I rearrange data as CMP gathers and develop hybrid-domain high-resolution parabolic Radon transforms which be used ether to interpolate null traces and extrapolate near and far offsets or suppress a strong interfere noise: multiples. I use it to attenuate multiples to verify performances of our algorithm and proposed routines for industrial application. Numerical examples and field data examples show a nice performance of our method.

Stimulus Familiarity Determines Recognition Strategy for Novel 3-D Objects

We describe a psychophysical investigation of the effects of object complexity and familiarity on the variation of recognition time and recognition accuracy over different views of novel 3D objects. Our findings indicate that with practice the response times for different views become more uniform and the initially orderly dependency of the response time on the distance to a "good" view disappears. One possible interpretation of our results is in terms of a tradeoff between memory needed for storing specific-view representations of objects and time spent in recognizing the objects.