Genotipagem das mutações do gene humano que codifica a lectina ligante da manose de voluntários do Departamento de Parasitologia do Instituto de Biociências

A leishmaniose é uma doença causada pelo protozoário do gênero Leishmania, possuindo uma variedade de formas clínicas. A mais severa é a Leishmaniose visceral, que no Brasil é causada por L. chagasi e transmitida pelo flebotomíneo Lutzomia longipalpis. O cão, tanto o doméstico como o selvagem, é o principal reservatório no ciclo zoonótico da doença, que no Brasil ocorre principalmente na região nordeste. Através de estudos epidemiológicos realizados em áreas endêmicas, notou-se que apesar do elevado número de pessoas infectadas, poucas manifestavam a doença. Isso pode ser explicado pelo estado nutricional e imunológico do paciente, infecção por HIV e variabilidade das cepas, visto que a heterogeneidade genética e diversidade clonal estão relacionadas a variações nos fatores de virulência do parasita. A lectina que se liga à manose (Mannose-Binding Lectin), ou MBL, é uma proteína que pode estar relacionada ao desenvolvimento da doença, uma vez que pode se ligar a carboidratos na membrana externa de patógenos, agindo como uma opsonina e facilitando assim a ação de macrófagos. Altas concentrações desta proteína podem ser desvantajosas, uma vez que pode facilitar a infecção por LV. Genes mutados podem contribuir para a variação do nível sérico da proteína diminuindo as taxas de transcrição do gene. Concluindo, o projeto tem o intuito de identificar e genotipar mutações específicas no promotor e no exon 1 do gene que codifica a MBL em humanos. A realização do estudo permitirá também, a consolidação de uma base de dados para estudos posteriores envolvendo a genética populacional da Lectina Ligante de Manose

Intertextualidades, símbolos e mitos entre o romance Mygale e o filme A pele que habito

The award-winning and controversial movie by Pedro Almodóvar “The skin I live” (2011) is an adaptation of Mygale’s novel (1984), the French writer Thierry Jonquet (1954-2009), translated into Portuguese in 2005 as Tarântula. It is a horror story, full of suspense, in which a renowned surgeon, Robert Ledgard, played by Antonio Banderas, switches, without any scruples, the sex of the young Vincent. What it shown to the viewer since the first images of the movie is, therefore, Vicente/Vera in her new and perfect female body. Flashbacks clarify during the movie the events that culminated in the opening scene that is presented to us, surprising us and, of course, shocking us. References to myths and symbols can be noticed in the movie. They bring with them, to be recognized by the viewer, issues related to the creation or metamorphosis, among others, as the Pygmalion and Galatea myth, which binds to artistic creation. Artistic metamorphosis operated equally by the filmmaker in his modern version of the doctor and the monster, for example, but, especially, in the rereading of the Jonquet’s novel. This study seeks to highlight some of the major myths and symbols inserted in Almódovar’s movie and what interpretations such insertions may ensue.

Um olhar perspectivo sobre a(s) cultura(s) caipira(s): reflexões a propósito da experiência de uma cidade do interior paulista

O texto apresenta os traços da cultura caipira, enfatizando a importância das consequências do comércio bovino para a sua circularidade pelos espaços urbanos do Centro Sul brasileiro. Além disso, propõe reflexões sobre as maneiras pelas quais as elites trataram as expressões desta cultura em dois momentos distintos: fins do século XIX e a onda nacionalista vigente após o governo de Getúlio Vargas, em 1930. Para tanto, parte da experiência histórica registrada em Barretos, cidade do interior paulista.

Estudo da influência do agente nucleante )ALTi5%B1%) na microestrutura de chapas AA8011 obtidas por lingotamento contínuo

The aluminum includes several properties with excellent relation between weight and mechanical resistance. With technological advances, increasingly demand the development of new alloys and other production processes in order to reduce the cost of production and insert these new alloys in broader applications. The process of continuous caster (TRC promoted the unite of the aluminum smelting process with the first stage of rolling, making it most economical through the merger these two phases besides transform the continuous casting process. The AA8xxx series is one of the most versatile aluminum alloys and the most often used in continuous caster process provided a great potential application in the market. In order to further, optimize the process it is necessary to increase awareness of the aluminum solidification phenomena associated with the addition of grain refiner, and control of some aluminum production parameters in the process (production rate, metal temperature, etc.). In this study, AA8011 alloy samples were taken in the raw state obtained by the continuous casting process. The samples were laminated to a thickness of 7mm during the process itself and analyzed at three points along its width by microstructural analysis throughout its thickness, the variation rate of addition of the grain refiner in order to assess the influence of this addition with crystallographic formation and some formation of intermetallic precipitates during the solidification. Through this work, it was possible to improve the knowledge related to the addition of refiner with the monitoring of these production processes

Analise comparativa do desempenho de ferramentas no torneamento do aluminio 2024

The aluminium alloys are used in many fields because of their versatility combined with the excellent aluminium’s properties, mentioned in the study. This study aims to compare the performance of polished Hard Metal, Hard Metal covered with TiB2 and High Speed Steel (HSS) tools, at the aluminium 2024 alloy’s turning, as a function of variation of some turning parameters such as: feed, depth of cut and cutting speed; and study the surface finish and the required power during turning by processing the output data, like analyze the chip’s features for each used tool. The results provide information of the tool’s material effects, when submitted to different turning conditions, about the output variable in question. In this way, it was possible to notice that although the Hard Metal covered with TiB2 tool has provided the better surface finish, the chip’s features were better when the turning was accomplished by the Polished Hard Metal tool. In relation to the required turning’s power, the lowest consumption occurred with the High Speed Steel tool

Efeito do recobrimento de filmes de carbono tipo diamante depositados a plasma na manutenção do torque de parafusos protéticos

Pós-graduação em Odontologia Restauradora - ICT

Análise funcional da proteína 14-3-3 de Paracoccidioides brasiliensis e prospecção de alvos terapêuticos inibidores da interação de P. brasiliensis à pneumócitos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Construção de um equipamento de ensaio de desgaste micro abrasivo por esfera rotativa fixa para análise do desgaste em revestimento duro aplicado por soldagem

Pós-graduação em Engenharia Mecânica - FEIS

Recuperação de turbinas hidráulicas danificadas por erosão cavitacional por soldagem com aço inoxidável/cobalto

Pós-graduação em Engenharia Mecânica - FEIS

Preparação e caracterização de nanocompósitos de Fe@SiO2, Fe@Fe3O4 e Fe3O4@PNIPAM

Pós-graduação em Química - IQ

Gonçalves Dias, o índio e a liberdade

This article presents a reading of the poem “O canto do guerreiro” written by Antonio Gonçalves Dias. The aim is to show the existence of a dialogue between the literary‘representation of the Brazilian‘indigenous and the concept of natural man, by Jean Jacques Rousseau. Going beyond a reading upon a romantic nationalism view, such dialogue makes explicit the poet’ conscience of his hole in Brazilian Romanticism and, at same time, gives a universal dimension to his poetic work.

Notas biográficas de João Augusto de Toledo

The research notes retrieve and review articles of Professor João Augusto de Toledo published in the Escola Normal Secundária de São Carlos Magazine. Altogether, the articles address issues of didactic teaching, national history, nationalist movement, psychology and experimental pedagogy, teacher training, organization of a primary school and childhood education. These topics are directly addressed to the person concerned.

Crise de hegemonia e emergência de novos atores na Bolívia: o governo de Evo Morales

The presidency of Evo Morales, indigenous leader and who heads the party Movement Towards Socialism (MAS), opens a series of transformations in several dimensions. The changes in socio-economic and political power express the critic of long-term coloniality relations between a dominant white elite and an indigenous subordinate majority that deepens after national independence. Following this perspective, present in sectors of support to the government, the strategy of the MAS cannot follow the tradition of social revolutions that operated structural breaks in the mode of production and the state organization, but points to a new decolonizing revolution, cultural and political, articulating an indigenism of broad nature, flexible and open to popular social movements. This view is facing critics in sectors of the left that identify the renewal of capitalist modernization process initiated in 1952 under the leadership of the Nationalist Revolutionary Movement (MNR), extending citizenship and democratizing access to the state for recognition of Indians as such. From this perspective, the transformations proposed by MAS tend to favor a system restoration by diversifying its economic and social base. From the contrast provided by these two lines of interpretation, we intend to analyze the structural possibilities of the strategy of the government of Evo Morales, taking as historical reference the transformations wrought by the nationalist revolution of 1952 and the neoliberal reforms initiated in 1980.

Herbst esplinte metálico fundido: novo modelo de confecção com Duralay

A má oclusão de Classe II é originária de vários fatores etiológicos e uma das características mais comum desta má oclusão é a retrusão mandibular3, por esse motivo, os aparelhos de avanço mandibular são eleitos para o tratamento e correção desta má oclusão. Um destes aparelhos é o Herbst, que apresenta várias formas de ancoragens na sua confecção10. Tradicionalmente, as bandas eram o sistema de ancoragem mais usado, no entanto, pela alta frequência de quebras as mesmas foram substituídas por esplinte metálico12. Esse aparelho quando confeccionado de cromo cobalto pelo método da prótese parcial removível, apresenta como desvantagens a quantidade de passos laboratoriais e a possível contração do metal, podendo ocasionar a má adaptação da estrutura metálica à boca do paciente. Dessa forma, este artigo apresenta um novo método de confecção do aparelho de Herbst esplinte metálico fundido com uso da Duralay e liga níquel cromo. Esse método diminui os passos laboratoriais, proporciona maior resistência à tração e minimiza a contração da estrutura metálica, pois a resina química Duralay proporciona menor expansão do que a cera2.

Influência do ciclo de sinterização da porcelana na adaptação marginal de infraestruturas de NiCrTi em função da biomecânica do preparo

A metal coping may undergo changes during porcelain firing, which compromises its marginal adaptation. The use of NiCrTi alloy proposes to minimize this effect through the high melting point of titanium present in its composition. This study evaluated the influence of porcelain firing cycle on the marginal adaptation of NiCrTi copings in different preparation designs. Forty standardized metal dies were fabricated with the following combinations finish line/convergence of the axial walls: 1) shoulder/6°; 2) shoulder/20°; 3) sloping shoulder/6°; 4) sloping shoulder/20°. On each die a metal ceramic restoration coping was made. The die/coping set was stabilized with orthodontic elastics, divided into four equidistant areas with three measurement points each and a cementation pressure was simulated. The measurements were taken under a stereomicroscope (32×). After the first measurement, the copings were submitted to sintering cycles simulating porcelain application. For repeated measurements, the same procedures described above were performed. Data were submitted to Student’s-t test, 1-way ANOVA and Tukey´s test (α = 0.05). Adaptation means (µm) before and after porcelain firing in different preparations were: 1) 111.92 and 127.31; 2) 124.15 and 135.48; 3) 122.19 and 138.77; 4) 166.09 and 186.72; respectively. The porcelain firing impaired adaptation, regardless of the preparation design. The preparation in a 20° sloping shoulder provided a worse adaptation when compared with preparations that had 6° and 20° shoulder, which were statistically equal. The 6° sloping shoulder was statistically equal to the other three preparation designs.