The putative Leishmania telomerase RNA (leishTER) undergoes trans-splicing and contains a conserved template sequence

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Serological detection of Toxoplasma gondii, Leishmania infantum and Neospora caninum in cats from an area endemic for leishmaniasis in Brazil

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Pathology of dogs in Campo Grande, MS, Brazil naturally co-infected with Leishmania infantum and Ehrlichia canis

Different parasites that commonly occur concomitantly can influence one another, sometimes with unpredictable effects. We evaluated pathological aspects of dogs naturally co-infected with Leishmania infantum and Ehrlichia canis. The health status of the dogs was investigated based on histopathological, hematological and biochemical analyses of 21 animals infected solely with L. infantum and 22 dogs co-infected with L. infantum and E. canis. The skin of both groups showed chronic, predominantly lymphohistioplasmacytic inflammatory reaction. The plasmacytosis in the lymphoid tissues was likely related with the hypergammaglobulinemia detected in all the dogs. The disorganization of extracellular matrix found in the reticular dermis of the inguinal region and ear, characterized by the substitution of thick collagen fibers for thin fibers, was attributed to the degree of inflammatory reaction, irrespective of the presence of parasites. In addition, the histopathological analysis revealed that twice as many dogs in the co-infected group presented Leishmania amastigotes in the ear skin than those infected solely with Leishmania, increasing the possibility of becoming infected through sand fly vectors. Our findings highlight the fact that the health of dogs infected concomitantly with L. infantum and E. canis is severely compromised due to their high levels of total plasma protein, globulins, alkaline phosphatase and creatine kinase, and severe anemia.

Molecular detection of Leishmania spp. in road-killed wild mammals in the Central Western area of the State of S��o Paulo, Brazil

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

First detection of Leishmania spp. DNA in Brazilian bats captured strictly in urban areas

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

The 2',4'-dihydroxychalcone could be explored to develop new inhibitors against the glycerol-3-phosphate dehydrogenase from Leishmania species

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Serological evidence of Infection by Leishmania (Leishmania) infantum (Synonym: Leishmania (Leishmania) chagasi) in free-ranging wild mammals in a nonendemic region of the state of S��o Paulo, Brazil

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Inclus��o escolar de crian��as com necessidades educacionais especiais: levantamento bibliogr��fico de teses, disserta����es e trabalhos de conclus��o de curso no acervo online da UNESP

Essa pesquisa teve como objetivo discutir e analisar a quest��o da inclus��o escolar tendo em vista explorar a produ����o acad��mica a respeito da tem��tica a fim de comparar as tend��ncias metodol��gicas de estudo e relatar as evolu����es hist��ricas ao longo desde a d��cada de 90. Para isso, o presente trabalho est�� estruturado em tr��s cap��tulos: o primeiro uma breve retrospectiva hist��rica cultural entre deficientes, escola e a sociedade; o segundo sobre a forma����o continuada e inicial do professor inclusivo e o terceiro descreve os resultados do levantamento bibliogr��fico realizado na base de dados online da UNESP, considerando trabalhos de conclus��o de curso, disserta����es e teses defendidas entre 1990 e 2013. Os dados coletados foram analisados qualitativamente, com alguns resultados quantitativos. Ap��s a coleta de dados, os trabalhos foram analisados com base nos seguintes crit��rios: objetivos, local da pesquisa, tipo de delineamento, instituto onde o trabalho foi apresentado, principais resultados, instrumentos e tipos de participantes (quando configurar uma pesquisa emp��rica). Pretendeu-se verificar, se existe uma tend��ncia na metodologia de trabalhos cient��ficos em rela����o a esse tema, de pesquisas mais de cunho bibliogr��fico ou emp��rico, dado a sua complexidade

Superdota����o e criatividade: an��lise de disserta����es e teses brasileiras

P��s-gradua����o em Educa����o para a Ci��ncia - FC

Tipologias de projetos de pesquisa de m��todos mistos: an��lise classificat��ria das teses de doutorado dos programas de p��s-gradua����o em geoci��ncias no Brasil

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Detec����o de co-infec����es por Leishmania (l.) chagasi, Trypanosoma evansi, Toxoplasma gondii e Neospora caninum em c��es

Co-infections by Leishmania (L.) chagasi, Trypanosoma evansi, Toxoplasma gondii and Neospora caninum in dogs were investigated. Amastigotes forms of Leishmania spp. were detected by cytopathological analysis of lymph nodes in 46,42% (39/84) of dogs. In a male dog, adult, without defined breed, from rural area and positive for Leishmania, were observed flagellated forms of T. evansi in blood smear. By immunofluorescence antibody test, 5,95% (5/84) of dogs were considered reactive to T. gondii, with titer equal to or higher than 1:64, while 3,57% (3/84) were reactive to N. caninum, with titer ���1:50. Among the animals with visceral leishmaniasis, one showed positive serological response to T. gondii and two for N. caninum. All dogs reactive to N. caninum were from rural area and the predominance of infection by T. gondii was in dogs from urban area. A young male dog from the rural area and seropositive for T. gondii showed Ehrlichia spp. morulae in the cytology and positive reaction for canine distemper virus. Thus, further studies are needed to assess the epidemiology of these infections in canine population, especially with respect to the reservoirs of Trypanosoma spp. in rural areas.

Leishmania sp. amastigotes identification in canine transmissible venereal tumor

Leishmaniasis is a vector-borne disease with Leishmania chagasi being the etiological agent of canine visceral leishmaniasis in South America. Canine venereal tumor is a transplantable round cell tumor of histiocytic origin which is mostly observed in sexually active male and female intact dogs. It has been shown that Leishmania amastigotes have higher tropism for the canine male genital tract tissues and venereal leishmaniasis transmission has been documented in dogs but, to date, a canine venereal tumor-dependent transmission route has not been fully demonstrated. In this report, a 10-year-old, mixed breed, intact female dog presented a vaginal venereal transmissible tumor but no other clinical abnormalities otherwise. Unexpectedly, tumor tissue imprint smears examination revealed Leishmania sp. amastigotes within infiltrating macrophages. In addition to the cytological direct identification, the protozoan was confirmed within the neoplastic tissue by means of immunohistochemistry and polymerase chain reaction. This report illustrates an asymptomatic Leishmania sp. infection that may have started on or from the canine venereal tumor tissue, the latter option further supporting previous evidence of such an alternative vector-independent route of transmission for canine visceral leishmaniasis in areas where these diseases coexist.

CD95 (FAS) and CD178 (FASL) induce the apoptosis of CD4+ and CD8+ cells isolated from the peripheral blood and spleen of dogs naturally infected with Leishmania spp

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Clinical and parasitological evaluation of dogs naturally infected by Leishmania (Leishmania) chagasi submitted to treatment with meglumine antimoniate and allopurinol

Aiming to assess the efficacy of the treatment, to verify the occurrence of possible disease relapses and to search for the presence of parasites after the treatment, seven dogs naturally infected by Leishmania sp., were submitted to a treatment with meglumine antimoniate and allopurinol. For this, lymph node and bone marrow aspiration biopsies were carried out at seven moments. After the end of the six-month observation period all dogs were submitted to euthanasia. Then, spleen and liver ���imprints��� and in vitro cultures were carried out to search for amastigote forms of the parasite. All animals presented remission of the symptoms and during all the observation period no dog presented relapse of the disease, although amastigote forms of the parasite were observed in two of the animals at the end of the experiment. Thus, it was possible to conclude that the treatment promotes clinical healing but it does not eliminate the parasites completely.

Leishmania sp. infection in dogs from Florian��polis, Santa Catarina, SC, Brazil

The aim of the present study was to investigate the occurrence of Leishmania sp. infection in dogs (N = 491) living in the municipality of Florian��polis, Santa Catarina (SC), Brazil, which was considered a disease-free region for visceral leishmaniasis until 2011, when autochthonous cases of canine disease were notified. Seroprevalence in this population was assessed by ELISA (0.4%; 2/491) and IFAT (4.09%; 24/491). Only one dog exhibited seroreactivity in both serological methods, comprising a total of 25 (5.3%) seroreagent animals. Leishmania sp. DNA, obtained from a sample of whole blood of this animal, was amplified by both conventional and Real-Time PCR. Sequencing of the amplified DNA and, thereby, determination of the Leishmania species involved, was not possible. Our results suggest the necessity of a thorough epidemiological investigation in Florian��polis. (AU).