Molecular detection of Leishmania spp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
03/12/2014
03/12/2014
16/06/2014
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 2008/09378-8 Processo FAPESP: 2008/08291-6 Background: Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens as Leishmania spp., offering new opportunities for epidemiological studies of this infection.Methods: This study aimed to evaluate the presence of Leishmania spp. and Leishmania chagasi DNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals.Results: DNA was detected in tissues of one Cavia aperea (Brazilian guinea pig), five Cerdocyon thous (crab-eating fox), one Dasypus septemcinctus (seven-banded armadillo), two Didelphis albiventris (white-eared opossum), one Hydrochoerus hydrochoeris (capybara), two Myrmecophaga tridactyla (giant anteater), one Procyon cancrivorus (crab-eating raccoon), two Sphiggurus spinosus (porcupine) and one Tamandua tetradactyla (lesser anteater) from different locations in the Central Western part of Sao Paulo state. The Leishmania chagasi DNA were confirmed in mesenteric lymph node of one Cerdocyon thous. Results indicated common infection in wild animals.Conclusions: The approach employed herein proved useful for detecting the environmental occurrence of Leishmania spp. and L. chagasi, as well as determining natural wild reservoirs and contributing to understand the host-parasite interaction. |
Formato |
01-07 |
Identificador |
http://dx.doi.org/10.1186/1678-9199-20-27 Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 20, p. 01-07, 2014. 1678-9199 http://hdl.handle.net/11449/130596 S1678-91992014000200331 WOS:000338584600002 S1678-91992014000200331.pdf |
Idioma(s) |
eng |
Publicador |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP |
Relação |
Journal of Venomous Animals and Toxins Including Tropical Diseases |
Direitos |
openAccess |
Palavras-Chave | #Road-killed animal #Leishmania spp #Leishmania chagasi #PCR #Zoonosis |
Tipo |
info:eu-repo/semantics/article |