Two “pillars” of cold-water coral reefs along Atlantic European margins: Prevalent association of Madrepora oculata with Lophelia pertusa, from reef to colony scale

The scleractinian coral Lophelia pertusa has been the focus of deep-sea research since the recognition of the vast extent of coral reefs in North Atlantic waters two decades ago, long after their existence was mentioned by fishermen. These reefs where shown to provide habitat, concentrate biomass and act as feeding or nursery grounds for many species, including those targeted by commercial fisheries. Thus, the attention given to this cold-water coral (CWC) species from researchers and the wider public has increased. Consequently, new research programs triggered research to determine the full extent of the corals geographic distribution and ecological dynamics of “Lophelia reefs”. The present study is based on a systematic standardised sampling design to analyse the distribution and coverage of CWC reefs along European margins from the Bay of Biscay to Iceland. Based on Remotely Operated Vehicle (ROV) image analysis, we report an almost systematic occurrence of Madrepora oculata in association with L. pertusa with similar abundances of both species within explored reefs, despite a tendency of increased abundance of L. pertusa compared to M. oculata toward higher latitudes. This systematic association occasionally reached the colony scale, with “twin” colonies of both species often observed growing next to each other when isolated structures were occurring off-reefs. Finally, several “false chimaera” were observed within reefs, confirming that colonial structures can be “coral bushes” formed by an accumulation of multiple colonies even at the inter-specific scale, with no need for self-recognition mechanisms. Thus, we underline the importance of the hitherto underexplored M. oculata in the Eastern Atlantic, re-establishing a more balanced view that both species and their yet unknown interactions are required to better elucidate the ecology, dynamics and fate of European CWC reefs in a changing environment.

Optimization of heterotrophic feeding of the soft coral Sarcophyton cf. glaucum

Heterotrophic feeding has an important role in the processes of growth and reproduction of mixotrophic corals. The soft coral Sarcophyton cf. glaucum is a good candidate for aquaculture due to its economic interest for the marine aquarium trade and for the bioprospection of marine natural products. The lack of information on heterotrophic feeding of this species with preserved microalgae conducted to development of this work. The present study aimed to evaluate the effect of the conservation processes of microalgae in its suitability as heterotrophic feeding for the mixotrophic coral S. cf. glaucum. Additionally, we aimed to identify the most suitable freeze-dried microalgae species and cell density to be employed in the culture of this mixotrophic coral species. Two experiments were performed: in the first experiment the microalgae Nannochloropsis oculata was supplied to coral fragments in three different preservation forms (live paste, frozen and freeze-dried) at the concentration of 106 cell mL-1; in the second experiment three different microalgae species (Nannochloropsis oculata, Isochrysis galbana and Phaeodactylum tricornutum) were tested in two different amounts: 7.33 mg L-1 (corresponding to the concentration of 106 cell mL-1 of Nannochloropsis oculata) and 3.66 mg L-1. Growth rate, survival, organic weight and photobiology of coral fragments, as well as water quality in culture tanks, were evaluated in both experiments. Preserved forms of microalgae did not demonstrated differences in growth rate, organic weight and survival rate of coral fragments, but affected water quality. Freeze-dried microalgae seems to be a good feed supply for coral aquaculture, as it has the best results and it has the higher shell-life time and the lower associated costs. Between the species evaluated in second experiment, Isochrysis galbana promoted higher specific growth rate and higher percentage of organic weight in the coral fragments; additionally the culture tanks supplied with this microalgae species also presented a better water quality in the end of the experiment.

Diurnal temporal patterns of the diversity and the abundance of reef fishes in a branching coral patch in New Caledonia

Small-scale spatial and temporal variability in animal abundance is an intrinsic characteristic of marine ecosystems but remains largely unknown for most animals, including coral reef fishes. In this study, we used a remote autonomous unbaited video system and recorded reef fish assemblages during daylight hours, 10 times a day for 34 consecutive days in a branching coral patch of the lagoon of New Caledonia. In total, 50 031 fish observations belonging to 114 taxa, 66 genera and 31 families were recorded in 256 recorded videos. Carnivores and herbivore-detritus feeders dominated the trophic structure. We found significant variations in the composition of fish assemblages between times of day. Taxa richness and fish abundance were greater in the early morning and in the late afternoon than during the day. Fourteen taxa displayed well-defined temporal patterns in abundance with one taxon influenced by time of day, six influenced by tidal state and seven influenced by both time of day and tidal state. None of these 14 taxa were piscivores, 10 were herbivore-detritus feeders, three were carnivores and one was plankton feeder. Our results suggest a diel migration from feeding grounds to shelter areas and highlight the importance of taking into account small-scale temporal variability in animal diversity and abundance when studying connectivity between habitats and monitoring communities.

Implementación de un detector de coral utilizando filtros Gabor Wavelets y máquinas de aprendizaje

Este trabajo se enfoca en la implementación de un detector de arrecife de coral de desempeño rápido que se utiliza para un vehículo autónomo submarino (Autonomous Underwater Vehicle, AUV, por sus siglas en inglés). Una detección rápida de la presencia de coral asegura la estabilización del AUV frente al arrecife en el menor tiempo posible, evitando colisiones con el coral. La detección de coral se hace en una imagen que captura la escena que percibe la cámara del AUV. Se realiza una clasificación píxel por píxel entre dos clases: arrecife de coral y el plano de fondo que no es coral. A cada píxel de la imagen se le asigna un vector característico, el mismo que se genera mediante el uso de filtros Gabor Wavelets. Éstos son implementados en C++ y la librería OpenCV. Los vectores característicos son clasificados a través de nueve algoritmos de máquinas de aprendizaje. El desempeño de cada algoritmo se compara mediante la precisión y el tiempo de ejecución. El algoritmo de Árboles de Decisión resultó ser el más rápido y preciso de entre todos los algoritmos. Se creó una base de datos de 621 imágenes de corales de Belice (110 imágenes de entrenamiento y 511 imágenes de prueba).

A gestão pedagógica no seio de uma instituição o caso do CAUÉ – Coral Alentejana da Universidade de Évora

Da criação, em 2004, da Secção Pedagógica do Coral Alentejano da Universidade de Évora, resultou a necessidade de encontrar um quadro organizacional que articulasse a participação dos(as) estudantes Universitários(as) (coralistas ou não), com as atividades próprias de um coral e com a extensão destas às escolas básicas. Desta forma, pretende-se promover o cante alentejano como ponto de partida e de chegada, no desenho e concretização curricular dos projetos educacionais. Assim sendo, surgiu a figura do Gestor Pedagógico concretizada, formalizada e legitimada, através da aprovação de um projeto de Estágio profissional, por parte do Instituto de Emprego e Formação Profissional. A comunicação que se apresenta descreve a atividade desenvolvida pela Gestora Pedagógica do Coral Alentejano da Universidade de Évora.

International workshop on "Different survey methods for coral reef fish, including methods based on underwater video"

In September 2013, staff from the University of the South Pacific (USP) Honiara campus, the Secretariat of the Pacific Community (SPC) and IFREMER (UR LEADNC, AMBIO project) in New Caledonia, and the French Institute for Pacific Coral Reefs (IRCP) in Moorea, French Polynesia, co-facilitated a workshop entitled “Different survey methods of coral reef fish, including the methods based on underwater video”. The workshop was attended by students from USP, NGO and fisheries officers. They were trained to several underwater visual census techniques and to the STAVIRO video-based technique, including both field work and data analysis.

Conclusiones y aportes a partir de una experiencia docente en el campo coral

Este artículo tiene como objetivo compartir la experiencia de un director coral con estudiantes de Licenciatura en música del Conservatorio del Tolima, considerando que los resultados de este trabajo se convierten en nuevos aprendizajes que aportan significativamente a la labor del mismo, a sus estudiantes y a pares académicos; esta actividad musical se ha realizado desde la asignatura que en la malla curricular de esta institución se denomina conjunto y está organizada en tres niveles -- Los contenidos que conforman la actividad coral, hacen considerar que el Repertorio es una de las herramientas pedagógicas más importantes en este trabajo debido a que contiene los elementos que contribuyen al proceso de formación musical de los integrantes de los conjuntos corales; por esta razón los aportes aquí expuestos harán especial énfasis sobre este aspecto

A Reeducação Postural Global (RPG) e as Autoposturas Respiratórias de Philippe Souchard, implicações na prática vocal/coral

A Investigação científica que se apresenta enquadra-se no âmbito do Projeto Educativo, integrado no Mestrado em Ensino da Música, especialidade em Música de Conjunto, realizado no Conservatório Superior de Música de Gaia (CSMG). Esta Investigação-ação, de análise qualitativa, centrou-se na implementação de um Programa de Intervenção: A Reeducação Postural Global (RPG) e As Autoposturas Respiratórias, na Produção Sonora Musical no Canto (Philippe Souchard, 1987; 1997; 2011). Assente no modelo de Relação Pedagógica (RP) de Renald Legendre (2005), teve como Sujeitos (S) de aprendizagem dois grupos das Classes de Conjunto de Coro, da Fundação Conservatório de Música de Gaia: o Grupo de Ação (GA) e o Grupo de Controlo (GC). Aplicadas sessões de fisioterapia ao GA, ambos os grupos interpretaram a Obra Coral Pange Lingua, de Zoltán Kodály. Pelos registos apresentados na análise de dados, ditos em diários de bordo dos Sujeitos (S) e do Agente (A) professor da Classe de Conjunto, e em imagens fílmicas e fotográficas realizadas, verificamos a eficácia deste Programa. À luz dos exercícios da Reeducação Postural Global (RPG) e das Autoposturas Respiratórias, de Philippe Souchard, aplicados ao estudo da obra mencionada, pudemos verificar que os Sujeitos (S) do Grupo de Ação apresentaram progressos vocais de significativa importância.

Auditoría de sistemas al cumplimiento de los requerimientos 7, 8, 9 de la norma PCIDSS aplicada a Coral Hipermercado, Racar plaza con corte a Junio de 2015

Coral Hipermercado GO es una empresa dedicada a la comercialización de diversos productos de consumo masivo. Debido a la demanda que mantienen y el nivel de transacciones que realizan en ventas diariamente, Coral Hipermercado GO no ha realizado anteriormente una auditoria de la Normativa PCI DSS, por lo que la implementación de la Normativa podría no ser completa o apropiada, las políticas de la empresa pueden no contener los procesos y procedimientos que se deben llevar a cabo, haciendo que la seguridad de accesos a los datos de los titulares de tarjetas se vean vulnerables, de igual manera el manejo de las autenticaciones y la infraestructura de la seguridad física, misma que permite el resguardo de los equipos y por ende de la información. Se observó en la auditoría realizada a los requerimientos 7, 8, 9 de la Normativa PCI DSS que la misma no ha sido socializada en su totalidad, existen falencias en su implementación ya que el uso de las autenticaciones, accesos a los datos y seguridad física no son correctos, viéndose expuesta la información de los datos de los titulares de tarjetas de pago y la posibilidad de incurrir en pérdidas debido a probables actos maliciosos que se podrían encontrar expuestos en un futuro. Es imperativo mencionar que la norma ayudara al cumplimiento de los objetivos siempre y cuando se logren disminuir las falencias de su implementación o caso contrario se procederá a dar las recomendaciones necesarias respecto a los problemas encontrados para posteriores correcciones de forma que se mejore la gestión de la empresa.

Antibacterial and cytotoxic properties of isoprenoids from the red sea soft coral, Lobophytum sp

Purpose: To evaluate the antibacterial and cytotoxic activities of the secondary metabolites of Lobophytum sp. Methods: Maceration with methanol: chloroform (1:1) was applied to extract the coral material. Chromatographic and spectroscopic techniques were employed for fractionation, isolation and elucidation of pure compounds. Antibacterial activities were performed by well diffusion method against three Gram-positive and four Gram-negative bacteria. Brine shrimp lethality test was employed to predict toxicity, while antitumor activity were tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) method against Ehrlich carcinoma cells. Results: Four sesquiterpenes, one cembranoid type diterpenes and two steroids were isolated. 1 exhibited significant antibacterial activity against four tested bacteria (P. aeruginosa, S. aureus, S. epidermis, and S. pneumonia) with MIC value of 15 μg/mL. Moreover, 1 showed high diameter zone of inhibition ranging from 16 - 18 mm against test bacteria. Compounds 4 and 5 displayed moderate antibacterial activity against all test bacteria with inhibition zone diameter (IZD) ranging from 11 – 15 mm and MIC values of 30 μg/mL. 2, 3, 6 and 7 exhibited weak antibacterial activity (IZD, 7 - 11 mm; MIC ≥ 30 μg/mL). In addition, only diterpene compound (4) showed high toxicity against A. Salina and antitumor activity against Erhlich carcinoma cells with the LD50 of 25 and 50 μg/mL, respectively. Conclusion: This study reveals the strong antibacterial activity of sesquiterpene alismol (1) and the potential antibacterial and antitumor activity of cembranoid type diterpene, cembrene A (4).

Investigating Stakeholders’ Preferences for Coral Reef Research Funding in Florida

Abstract: Heavily used and highly valuable, the Florida Reef is one of the world's most threatened ecosystems. Stakeholders from a densely urbanized coastal region in proximity to the reef system recognize its degradation, but their comprehension of climate change and commitment to pay for sustainable management and research funding have been opaque. With an emphasis on recreational anglers, residential stakeholders were surveyed online about their marine activities, perceptions of resources and threats, and willingness to pay (WTP) for dedicated coral reef research funding in Florida. The majority of stakeholders are wealthy, well educated, and politically independent. Supermajorities favored the two scenarios of taxation for a Florida Coral Reef Research Fund, and the scenario with matching federal funds earned higher support. In regression analyses, several factors emerged as significant contributors to stakeholders’ preferences, and the four recurring factors in extended models were prioritizing the environment over the economy, donating to environmental causes, concern about coral reefs, and concern about climate change, with the latter indicating a recent shift of opinion. Status in terms of income and education were found insignificant, and surprisingly income was negatively correlated with WTP. Perceptions through lenses of environmental and emotional attachments appear to overwhelm conventional status-based factors. Applied statewide, the first scenario's extrapolated WTP (based on a sales tax rate of 2.9%) would generate $675 million annually, and the extrapolated WTP under the second scenario, with matching federal funds (based on a sales tax rate of 3.0%) would generate $1.4 billion. Keywords: willingness to pay, coral reef research, taxation, climate change, stakeholder, perceptions, Florida Reef, recreational fishing, anglers

Quorum Sensing and Microbial Interactions in Coral Black Band Disease and Coral-Associated Bacteria

The black band disease (BBD) microbial consortium often causes mortality of reef-building corals. Microbial chemical interactions (i.e., quorum sensing (QS) and antimicrobial production) may be involved in the BBD disease process. Culture filtrates (CFs) from over 150 bacterial isolates from BBD and the surface mucopolysaccharide layer (SML) of healthy and diseased corals were screened for acyl homoserine lactone (AHL) and Autoinducer-2 (AI-2) QS signals using bacterial reporter strains. AHLs were detected in all BBD mat samples and nine CFs. More than half of the CFs (~55%) tested positive for AI-2. Approximately 27% of growth challenges conducted among 19 isolates showed significant growth inhibition. These findings demonstrate that QS is actively occurring within the BBD microbial mat and that culturable bacteria from BBD and the coral SML are able to produce QS signals and antimicrobial compounds. This is the first study to identify AHL production in association with active coral disease.

Effects of Multiple Ecological Drivers on Recruitment and Succession of Coral Reef Macroalgal Communities

The study evaluated the effects of herbivory pressure, nutrient availability and potential propagule supply on recruitment and succession of coral reef macroalgal communities. Recruitment and succession tiles were placed in a nutrient-herbivory factorial experiment and macroalgal abundances were evaluated through time. Proportional abundances of macroalgal form-functional groups on recruitment and succession tiles were similar to field established communities within treatments, evidencing possible effects of adult macroalgae as propagule supply. Macroalgal abundance of recruitment tiles increased with nutrient loading and herbivory reduction combined whereas on succession tiles nutrient loading increased abundance of articulated-calcareous only when herbivores were excluded. Macroalgal field established communities were only affected by herbivory reduction.

El cuarto canto coral en Oedipus de Séneca : (vv.882-904)

Fil: Pedernera, Soledad. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.