A Combined Molecular Cloning and Mass Spectrometric Method to Identify, Characterize, and Design Frenatin Peptides from the Skin Secretion of Litoria infrafrenata

Amphibian skin secretions are unique sources of bioactive molecules, particularly bioactive peptides. In this study, the skin secretion of the white-lipped tree frog (Litoria infrafrenata) was obtained to identify peptides with putative therapeutic potential. By utilizing skin secretion-derived mRNA, a cDNA library was constructed, a frenatin gene was cloned and its encoded peptides were deduced and confirmed using RP-HPLC, MALDI-TOF and MS/MS. The deduced peptides were identified as frenatin 4.1 (GFLEKLKTGAKDFASAFVNSIKGT) and a post-translationally modified peptide, frenatin 4.2 (GFLEKLKTGAKDFASAFVNSIK.NH2). Antimicrobial activity of the peptides was assessed by determining their minimal inhibitory concentrations (MICs) using standard model microorganisms. Through studying structure–activity relationships, analogues of the two peptides were designed, resulting in synthesis of frenatin 4.1a (GFLEKLKKGAKDFASALVNSIKGT) and frenatin 4.2a (GFLLKLKLGAKLFASAFVNSIK.NH2). Both analogues exhibited improved antimicrobial activities, especially frenatin 4.2a, which displayed significant enhancement of broad spectrum antimicrobial efficiency. The peptide modifications applied in this study, may provide new ideas for the generation of leads for the design of antimicrobial peptides with therapeutic applications.

Search for Pair-Produced Supersymmetric Top Quark Partners with the ATLAS Experiment

Searches for the supersymmetric partner of the top quark (stop) are motivated by natural supersymmetry, where the stop has to be light to cancel the large radiative corrections to the Higgs boson mass. This thesis presents three different searches for the stop at √s = 8 TeV and √s = 13 TeV using data from the ATLAS experiment at CERN’s Large Hadron Collider. The thesis also includes a study of the primary vertex reconstruction performance in data and simulation at √s = 7 TeV using tt and Z events. All stop searches presented are carried out in final states with a single lepton, four or more jets and large missing transverse energy. A search for direct stop pair production is conducted with 20.3 fb−1 of data at a center-of-mass energy of √s = 8 TeV. Several stop decay scenarios are considered, including those to a top quark and the lightest neutralino and to a bottom quark and the lightest chargino. The sensitivity of the analysis is also studied in the context of various phenomenological MSSM models in which more complex decay scenarios can be present. Two different analyses are carried out at √s = 13 TeV. The first one is a search for both gluino-mediated and direct stop pair production with 3.2 fb−1 of data while the second one is a search for direct stop pair production with 13.2 fb−1 of data in the decay scenario to a bottom quark and the lightest chargino. The results of the analyses show no significant excess over the Standard Model predictions in the observed data. Consequently, exclusion limits are set at 95% CL on the masses of the stop and the lightest neutralino.

Measurement of the Z and W production cross section in pp collisions at LHC using a bayesian approach

L’obiettivo di tutto il mio lavoro è stato quello di misurare le sezioni d’urto di produzione dei bosoni deboli W ± e Z nei loro decadimenti leptonici (e, μ) coi dati raccolti dal rivelatore ATLAS a LHC con un’energia del centro di massa di √s = 13 TeV relativi all’estate 2015. Gli eventi selezionati sono gli stessi di quelli del recente articolo della Collaborazione ATLAS sullo stesso argomento, in modo anche da poter operare un confronto tra i risultati ottenuti. Confronto peraltro necessario, poichè i risultati sono stati ottenuti con due metodologie differenti: tradizionale (classica) per l’articolo, bayesiana in questa tesi. L’approccio bayesiano permette di combinare i vari canali e di trattare gli effetti sistematici in modo del tutto naturale. I risultati ottenuti sono in ottimo accordo con le predizioni dello Standard Model e con quelli pubblicati da ATLAS.

Myths and Realities of Inter-professional Working

Introduction: Childhood cancers are rare and community based health care professionals have limited experience in caring for these children and often even less experience in providing their palliative care. It is well recognised that the provision of palliative care falls beyond the remit of any one profession, thus inter professional working is the standard model. This qualitative study aims to examine the experiences of the range of health care professionals involved in providing palliative care at home for children with cancer, focusing on how knowledge is exchanged; the level of communication and support both interprofessionally and at the community/specialist interface. It also aims to examine interprofessional collaboration in palliative care; identifying healthcare professional's perceptions of problems involved, interprofessional boundaries, specific areas of the organisation or provision of care that could be enhanced through changes in practice, support issues and the educational needs of health professionals. Methods The study involves three types of data collection; in-depth interviews, facilitated case discussion (FCD) and field notes from up to 20 cases (a "case" refers to the provision of palliative care to one child). Cases are selected from children who were treated at one regional childhood caner centre. For each case the community based health care professionals (for example the GP, community nurse or health visitor) involved in the care of the child at home are invited to participate in a one-to-one tape recorded in-depth interview followed by a group discussion in the form of a FCD. Field notes are completed following each interview. Data analysis follows a grounded theory approach. The term "social worlds theory" (SWT) his used to define a type of social organisation with no fixed or formal boundaries (such as membership boundaries), for example the range of health professionals that work together to provide palliative care. The boundaries of SW's are determined by the interaction and communication between recognised organisations, such as community nursing teams and general practitioners. SWT examines encounters between different professional groups and can be used to extend knowledge in both the organisation (for example general practice) and the content of what is being provisioned (for example, palliative care). The use of SWT in the analysis of the data is through examining the ethos of the different professions and the associated individual approaches to palliative care, exploring how this determines their roles in the provision of palliative care. Results 10 cases have so far been completed: 47 1:1 interviews (with a range of between 2-7 health care professionals being involved in each case): ( 9 x GP, 19 x CCN, 4 x DN, 3 x HV, 1 x HV assistant 7 x paediatric palliative care nurses, 1 x home support worker, 1 x OT, 1 x physiotherapist, 1 x community paediatrician) and 5 x FCD. The range of participants in the FCDs reflected that of the individual interview sampler. Data obtained to date gives clear insight into the personal experience of the individual health care professional in providing palliative care. Two themes emerging from the data will be focused upon: the continuity of care provision throughout treatment and palliation and the emotional burden experiences by the health care professional. Conclusions SWT can provide a useful framework in examining the social worlds of a disparate group of health care professionals working together for the first and maybe, the only time. A wide variation in the continuity of care provision has been found not only between professions, but also within professions. The emotional burden is evident across the professions.

Topics in Behavioural Economics - Information and Fairness. Revised edition

This thesis dwells upon topics in behavioural economics: information and fairness, with five research papers. The first two contributions are concerned with the extension of standard auction formats with information acquisition strategies. The third paper addresses global games framed as a speculative attack and tests theoretical predictions for risk and ambiguity. The fourth contribution deals with disclosing conflicts of interest, where one player has a monetary incentive to deceive. The last paper extends a standard model of social preferences with a second fairness dimension and studies how economic agents distort fairness norms exhibiting a self-serving bias effect.

Critère de validation croisée pour le choix des modèles des petits domaines au niveau des unités

Ce mémoire s’intéresse à l’étude du critère de validation croisée pour le choix des modèles relatifs aux petits domaines. L’étude est limitée aux modèles de petits domaines au niveau des unités. Le modèle de base des petits domaines est introduit par Battese, Harter et Fuller en 1988. C’est un modèle de régression linéaire mixte avec une ordonnée à l’origine aléatoire. Il se compose d’un certain nombre de paramètres : le paramètre β de la partie fixe, la composante aléatoire et les variances relatives à l’erreur résiduelle. Le modèle de Battese et al. est utilisé pour prédire, lors d’une enquête, la moyenne d’une variable d’intérêt y dans chaque petit domaine en utilisant une variable auxiliaire administrative x connue sur toute la population. La méthode d’estimation consiste à utiliser une distribution normale, pour modéliser la composante résiduelle du modèle. La considération d’une dépendance résiduelle générale, c’est-à-dire autre que la loi normale donne une méthodologie plus flexible. Cette généralisation conduit à une nouvelle classe de modèles échangeables. En effet, la généralisation se situe au niveau de la modélisation de la dépendance résiduelle qui peut être soit normale (c’est le cas du modèle de Battese et al.) ou non-normale. L’objectif est de déterminer les paramètres propres aux petits domaines avec le plus de précision possible. Cet enjeu est lié au choix de la bonne dépendance résiduelle à utiliser dans le modèle. Le critère de validation croisée sera étudié à cet effet.

Produção de charginos no ilc

O principal objetivo desta dissertação é a produção de charginos (partículas supersimétricascarregadas) leves no futuro acelerador internacional linear de e +e− (ILC) para diferentescenários de quebra de supersimetria. Charginos são partículas constituídas pela mistura docampo Wino carregado com o Higgsino carregado. A principal motivação para se estudar teorias supersimétricas deve-se ao grande número de problemas do Modelo Padrão (SM) que esta consegue solucionar, entre eles: massa dos neutrinos, matéria escura fria e o ajuste-fine (finetuning). Além disso, estudamos os princípios fundamentais que norteam a física de partículas,isto é, o princípio de gauge e o mecanismo de Higgs.

A durabilidade dos clássicos do design como instrumento de apoio ao processo de conceção de produto: 10 princípios para o projeto

A presente investigação identifica o caminho seguido no estudo que se centrou na durabilidade dos produtos como estratégia para a prevenção/redução dos impactes ambientais provocados pela excessiva produção e consumo das sociedades ocidentais. O trabalho baseou-se primeiramente na reunião de argumentos que justificassem a necessidade da investigação. A partir dos conhecimentos obtidos sobre as consequências do descarte prematuro de produtos, incluindo a produção de resíduos e utilização de recursos naturais, a investigação foi direcionada para as estratégias que motivam a redução do consumo pelo aumento da vida útil do produto. A durabilidade de alguns produtos designados de Clássicos do Design motivou o desenvolvimento da investigação. Depois de definido o universo que se enquadra nesta categoria, foi selecionada uma amostra que se considerou representativa e criou-se uma base de dados onde se sistematizou os conteúdos relevantes a conhecer. Através da análise qualitativa e quantitativa da amostra desses produtos, obteve-se uma matriz operativa composta por 10 princípios que pode ser introduzida no processo de design de novos produtos para aquisição de um tempo de vida útil inicial potencialmente maior. Os resultados da aplicação prática da estratégia desenvolvida, a tese, determinarão no futuro a conceção e produção de artefactos que se pretende apresentar à indústria nacional.

Measurement of Wγγ Cross Section and Limits on Anomalous Quartic Gauge Couplings in Proton-Proton Collisions at 8 TeV with the CMS detector

Present the measurement of a rare Standard Model processes, pp →W±γγ for the leptonic decays of the W±. The measurement is made with 19.4 fb−1 of 8 TeV data collected in 2012 by the CMS experiment. The measured cross section is consistent with the Standard Model prediction and has a significance of 2.9σ. Limits are placed on dimension-8 Effective Field Theories of anomalous Quartic Gauge Couplings. The analysis has particularly sensitivity to the fT,0 coupling and a 95% confidence limit is placed at −35.9 < fT,0/Λ4< 36.7 TeV−4. Studies of the pp →Zγγ process are also presented. The Zγγ signal is in strict agreement with the Standard Model and has a significance of 5.9σ.

Higgs production in association with top quarks at the LHC

Since it has been found that the MadGraph Monte Carlo generator offers superior flavour-matching capability as compared to Alpgen, the suitability of MadGraph for the generation of ttb¯ ¯b events is explored, with a view to simulating this background in searches for the Standard Model Higgs production and decay process ttH, H ¯ → b ¯b. Comparisons are performed between the output of MadGraph and that of Alpgen, showing that satisfactory agreement in their predictions can be obtained with the appropriate generator settings. A search for the Standard Model Higgs boson, produced in association with the top quark and decaying into a b ¯b pair, using 20.3 fb−1 of 8 TeV collision data collected in 2012 by the ATLAS experiment at CERN’s Large Hadron Collider, is presented. The GlaNtp analysis framework, together with the RooFit package and associated software, are used to obtain an expected 95% confidence-level limit of 4.2 +4.1 −2.0 times the Standard Model expectation, and the corresponding observed limit is found to be 5.9; this is within experimental uncertainty of the published result of the analysis performed by the ATLAS collaboration. A search for a heavy charged Higgs boson of mass mH± in the range 200 ≤ mH± /GeV ≤ 600, where the Higgs mediates the five-flavour beyond-theStandard-Model physics process gb → tH± → ttb, with one top quark decaying leptonically and the other decaying hadronically, is presented, using the 20.3 fb−1 8 TeV ATLAS data set. Upper limits on the product of the production cross-section and the branching ratio of the H± boson are computed for six mass points, and these are found to be compatible within experimental uncertainty with those obtained by the corresponding published ATLAS analysis.

All one-loop scalar vertices in the effective potential approach

Using the one-loop Coleman-Weinberg effective potential, we derive a general analytic expression for all the derivatives of the effective potential with respect to any number of classical scalar fields. The result is valid for a renormalisable theory in four dimensions with any number of scalars, fermions or gauge bosons. This result corresponds to the zero-external momentum contribution to a general one-loop diagram with N scalar external legs. We illustrate the use of the general result in two simple scalar singlet extensions of the Standard Model, to obtain the dominant contributions to the triple couplings of light scalar particles under the zero external momentum approximation.

Beyond the Cosmological Principle

Simplifying the Einstein field equation by assuming the cosmological principle yields a set of differential equations which governs the dynamics of the universe as described in the cosmological standard model. The cosmological principle assumes the space appears the same everywhere and in every direction and moreover, the principle has earned its position as a fundamental assumption in cosmology by being compatible with the observations of the 20th century. It was not until the current century when observations in cosmological scales showed significant deviation from isotropy and homogeneity implying the violation of the principle. Among these observations are the inconsistency between local and non-local Hubble parameter evaluations, baryon acoustic features of the Lyman-α forest and the anomalies of the cosmic microwave background radiation. As a consequence, cosmological models beyond the cosmological principle have been studied vastly; after all, the principle is a hypothesis and as such should frequently be tested as any other assumption in physics. In this thesis, the effects of inhomogeneity and anisotropy, arising as a consequence of discarding the cosmological principle, is investigated. The geometry and matter content of the universe becomes more cumbersome and the resulting effects on the Einstein field equation is introduced. The cosmological standard model and its issues, both fundamental and observational are presented. Particular interest is given to the local Hubble parameter, supernova explosion, baryon acoustic oscillation, and cosmic microwave background observations and the cosmological constant problems. Explored and proposed resolutions emerging by violating the cosmological principle are reviewed. This thesis is concluded by a summary and outlook of the included research papers.

Desenvolvimento de fontes radioativas seladas imobilizadas em resina epóxi para verificação de detectores utilizados em medicina nuclear

Dissertação (Mestrado em Tecnologia Nuclear)

Using the Higgs to Probe Naturalness

The extreme sensitivity of the mass of the Higgs boson to quantum corrections from high mass states, makes it 'unnaturally' light in the standard model. This 'hierarchy problem' can be solved by symmetries, which predict new particles related, by the symmetry, to standard model fields. The Large Hadron Collider (LHC) can potentially discover these new particles, thereby finding the solution to the hierarchy problem. However, the dynamics of the Higgs boson is also sensitive to this new physics. We show that in many scenarios the Higgs can be a complementary and powerful probe of the hierarchy problem at the LHC and future colliders. If the top quark partners carry the color charge of the strong nuclear force, the production of Higgs pairs is affected. This effect is tightly correlated with single Higgs production, implying that only modest enhancements in di-Higgs production occur when the top partners are heavy. However, if the top partners are light, we show that di-Higgs production is a useful complementary probe to single Higgs production. We verify this result in the context of a simplified supersymmetric model. If the top partners do not carry color charge, their direct production is greatly reduced. Nevertheless, we show that such scenarios can be revealed through Higgs dynamics. We find that many color neutral frameworks leave observable traces in Higgs couplings, which, in some cases, may be the only way to probe these theories at the LHC. Some realizations of the color neutral framework also lead to exotic decays of the Higgs with displaced vertices. We show that these decays are so striking that the projected sensitivity for these searches, at hadron colliders, is comparable to that of searches for colored top partners. Taken together, these three case studies show the efficacy of the Higgs as a probe of naturalness.

Estabelecimento de um sistema padrão primário para raios X baixas com uma câmara de ionização de ar livre de energias

Dissertação (Mestrado em Tecnologia Nuclear)