Bacteriological investigations of prawn canneries 2. Incidence of Clostridium perfringens

Prawn processing factories of the three major fish processing centres of the West Coast of India, viz., Cochin, Mangalore and Calicut were surveyed to determine the occurrence of Clostridium perfringens in processing areas, and in processed products. Direct plating on Sulphite-polymyxin- sulphadiazine Agar and enrichment techniques were used. Samples of prawn, prawn guts, frozen prawns, canned prawns, water, ice, swab from utensils and soil from the factory premises were examined. Among a total of 461 samples examined, only 32 (6.9%) gave positive results. The incidence of C. perfringens was more in prawn guts (80%), followed by soil (50%), prawn (38%), ice (33.3%), frozen prawns (11%), swab (5.0%) and water (1.1%). No C. perfringens was isolated from canned prawns.

Biochemical investigations on the edible molluscs of Kerala 2. A study on the nutritional value of some gastropods and cephalopods

Data on the biochemical composition and food value of the edible portions of two gastropods, Pila virens and Achatina fulica and two cephalopods, Sepiella inermis and Loigo indica have been presented. These molluscs possess nutritive meat very rich in protein and minerals, which compare favourably with popular food fishes in caloric value. The significance of the variations met with in the biochemical constituents of the different species has been discussed.

Investigations on long distance transportation of fish 1. Transportation of frozen fish from Cochin to Calcutta

Oil Sardine (Sardinella longiceps), mackerel (Rastrelliger kanagurta), cat fish (Arius sp.), threadfin bream (Nemipterus japonicus) and ribbon fish (Trichurus sp.) were frozen in glazed/unglazed blocks, packed in expanded polystyrene (EPS) insulated plywood boxes with and without additional ice and despatched in uninsulated parcel vans of trains from Cochin to Calcutta. The consignments reached the destination in excellent condition and were readily disposed off.

Investigations on the number of trials required for statistical comparison of fishing gears

The number of fishing trials required for comparing the efficiency of fishing gears was investigated. A unique solution to this problem did not appear to exist because of the heterogeneity of the experimental material. Sequential experimentation and analysis have been found to be a practical approach to this problem. By this, the experiment can be terminated almost after 35 days fishing for catches with standard error per unit as per cent of the mean about 30% or less (after logarithmic transformation). For data with mean catches less than 1.5 kg analysis of variance approach does not appear to be meaningful.

Molecular dynamics simulation on the complex of the tobramycin and 16S rRNA a site

A 3. 6 ns molecular dynamics simulation was carried out on the complex system of tobramycin and 16S rRNA in order to understand the speciality recognition mechanism between tobramycin and 16S rRNA at the molecular level. The results demonstrate that two l

Secondary structural analysis of the mRNA regions encoding the hemagglutinin cleavage site basic amino acids of the avian influenza virus H5N1 subtype samples

Here we report the codon bias and the mRNA secondary structural features of the hemagglutinin (HA) cleavage site basic amino acid regions of avian influenza virus H5N1 subtypes. We have developed a dynamic extended folding strategy to predict RNA secondar

Site-directed PEGylation of trichosanthin retained its anti-HIV activity with reduced potency in vitro

Trichosanthin (TCS) was the first ribosome inactivating protein found to possess anti-HIV-1 activity. Phase I/II clinical trial of this compound had been done. Antigenicity and short plasma half-life were the major side effects preventing further clinical trial. Modification of TCS is therefore necessary to revive the interest to develop this compound as an anti-HIV agent. Three potential antigenic sites (Ser-7, Lys-173, and Gln-219) were identified by computer modeling. Through site-directed mutagenesis, these three antigenic amino acids were mutated to a cysteine residue resulting in 3 TCS mutants, namely S7C, K173C, and Q219C. These mutants were further coupled to polyethylene glycol with a molecular size of 20 kDa (PEG) via the cysteine residue. This produced another three TCS derivatives, namely PEG(20)k-S7C, PEG(20)k-K173C, and PEG(20)k-Q219C. PEGylation had been widely used recently to decrease immunogenicity by masking the antigenic sites and prolong plasma half-life by expanding the molecular size. The in vitro anti-HIV-1 activity of these mutants and derivatives was tested. Results showed that the anti-HIV-1 activity of S7C, K173C, and Q219C was decreased by about 1.5- to 5.5-fold with slightly lower cytotoxicity. On the other hand, PEGylation produced larger decrease (20- to 30-fold) in anti-HIV activity. Cytotoxicity was, however, weakened only slightly by about 3-fold. The in vitro study showed that the anti-HIV activity of PEGylated TCS was retained with reduced potency. The in vivo activity is expected to have only slightly changed due to other beneficial effects like prolonged half-life. (C) 2004 Elsevier Inc. All rights reserved.

Symmetrical 1-pyrrolidineacetamide showing anti-HIV activity through a new binding site on HIV-1 integrase

Aim: To characterize the functional and pharmacological features of a symmetrical 1-pyrrolidineacetamide, N,N'-(methylene-di-4,1-phenylene) bis-1-pyrrolidineacetamide, as a new anti-HIV compound which could competitively inhibit HIV-1 integrase (IN) bindi

Co-management approach on fisher group: a case study on Ramsar site, Tanguar haor in Bangladesh

In Bangladesh, wetlands are managed through leasing system traditionally from time immemorial. Recently the Government accepted co-management approach for wetland fisheries management and this approach is being practiced in few wetlands for maximize revenue income. A study was carried out to evaluate trend and impact of co-management in Tanguar haor (a Ramsar site wetland) on fisheries resources and livelihood of resident people in the immediate vicinity of the wetland. In Tanguar haor, conflict between leaseholders and the local community was a common phenomenon in the past. Since 2003 the district administration of Sunamganj has been managing the vast wetland resources, however, local people participation was ignored in haor management system. Average monthly fish catch of fishermen increased by 17% after introduction of co-management system and 7 fish species reappeared after introduction of co-management. Average monthly volume of fish catch has increased from 70 kg to 87 kg. A well-defined management structure has been developed for integration of all people of Tanguar haor which would enable them to raise voice jointly and influence policy in their favour.

A carp hatchery: its essential components, site, proper hatchery operation procedures

The paper presents the essential components, ideal site, and proper hatchery operation procedures of a carp hatchery.

Nest site selection by Black-necked Crane Grus nigricollis in the Ruoergai Wetland, China

From 5 May 2003 to early June 2005, nest site selection of Black-necked Cranes Grits nigricollis was studied at the Ruoergai Wetland Nature Reserve (RWNR), an important breeding area for the species in China. Results showed that the crane nests only in we