982 resultados para Engineering, Civil


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This article presents results of an experimental investigation on the resistance to chemical attack (with sulphuric, hydrochloric and nitric acid) of several materials: OPC concrete, high-performance concrete, epoxy resin, acrylic painting and a fly ash-based geopolymeric mortar). Three types of acids with three high concentrations (10, 20 and 30%) were used to simulate long-term degradation. A cost analysis was also performed. The results show that the epoxy resin has the best resistance to chemical attack independently of the acid type and the acid concentration. However, the cost analysis shows that the epoxy resin-based solution is the least cost-efficient solution being 70% above the cost efficiency of the fly ash-based geopolymeric mortar.

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Dissertação de mestrado integrado em Engenharia Civil

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Tese de Doutoramento em Ciências (Especialidade de Física)

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Degree of Doctor of Philosophy of Structural/Civil Engineering

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Dissertação de mestrado integrado em Engenharia Civil

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Kinetic models have a great potential for metabolic engineering applications. They can be used for testing which genetic and regulatory modifications can increase the production of metabolites of interest, while simultaneously monitoring other key functions of the host organism. This work presents a methodology for increasing productivity in biotechnological processes exploiting dynamic models. It uses multi-objective dynamic optimization to identify the combination of targets (enzymatic modifications) and the degree of up- or down-regulation that must be performed in order to optimize a set of pre-defined performance metrics subject to process constraints. The capabilities of the approach are demonstrated on a realistic and computationally challenging application: a large-scale metabolic model of Chinese Hamster Ovary cells (CHO), which are used for antibody production in a fed-batch process. The proposed methodology manages to provide a sustained and robust growth in CHO cells, increasing productivity while simultaneously increasing biomass production, product titer, and keeping the concentrations of lactate and ammonia at low values. The approach presented here can be used for optimizing metabolic models by finding the best combination of targets and their optimal level of up/down-regulation. Furthermore, it can accommodate additional trade-offs and constraints with great flexibility.

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Dissertação de mestrado integrado em Engenharia Civil

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Dissertação de mestrado integrado em Engenharia Civil

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Dissertação de mestrado integrado em Engenharia Civil

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Dissertação de mestrado integrado em Engenharia Civil

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Dissertação de mestrado integrado em Engenharia Civil

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Dissertação de mestrado integrado em Engenharia Civil

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Dissertação de mestrado integrado em Civil Engineering

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Dissertação de mestrado integrado em Engenharia Civil