999 resultados para Deficiência de vitamina E
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The identification of genotypes for drought tolerance has a great importance in breeding programs. The aim of this study was to characterize genotypes of beans in response to drought tolerance in different reproductive stages through physiologic, agronomic and molecular analysis. The experiment was conducted in greenhouse, using a randomized block design with four replicates; 10 cultivars: ANFC 9, ANFP 110, BRS Esplendor, BRSMG Realce, IPR Siriri, IPR Tangará, IPR Tuiuiu, IPR Uirapuru, IAC Imperador and IAC Milênio under two conditions of irrigation: plants irrigated during their entire life cycle, and plants under irrigation suppression in the reproductive stage (R7) until 16% of field capacity, when the irrigation was restored. In the last four days of stress, the gas exchanges were analyzed, and in the last day of stress was analyzed the percentage of closed stomata in the abaxial surface of the leaves, collected in different times of the day (9h, 12h, 15h and 18h). Additionally, plant samples were collected for the following analysis: fresh and dry mass of leaves, stems and legumes, and proline content in leaves and roots. The plants were harvested at the physiological maturity and the yield components and grain yield were determined. In addition, in order to identify polymorphisms in the sequences of promoters and genes related to drought, seven pairs of primers were tested on the group of genotypes. The drought susceptibility indexes (ISS) ranged from 0.65 to 1.10 in the group of genotypes, which the lowest values observed were for IAC Imperador (0.65) and BRS Esplendor (0.87), indicating the ability of these two genotypes to maintain grain yield under water stress condition. All genotypes showed reduction in yield components under water stress. IAC Imperador (43.4%) and BRS Esplendor (60.6%) had the lowest reductions in productivity and kept about 50% of the stomata closed during all the different times evaluated at last day of irrigation suppression. IAC Imperador showed greater water use efficiency and CO2 assimilation rate under drought stress. IPR Tuiuiú, IPR Tangará and IAC Imperador had the highest proline concentrations in the roots. Under water stress condition, there was a strong positive correlation (0.696) between the percentage of stomata closed with the number of grains per plant (0.696) and the fresh mass of leaves (0.731), the maximum percentage of stomata closed 73.71% in water stress. The accumulation of proline in the root was the character that most contributed to the divergence between the genotypes under water deficit, but not always the genotypes that have accumulated more proline were the most tolerant. The polymorphisms in DNA of coding and promoting sequences of transcription factors studied in this experiment did not discriminate tolerant genotypes from the sensitive ones to water stress.
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Dissertação de Mestrado apresentada ao ISPA - Instituto Universitário
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Vitamin A is important in many essential body processes and its deficiency results in serious consequences for human health. Breast milk is the only source of this vitamin for children that are exclusively breastfed. Analysis of vitamin A in mother s milk is important because its concentration is related to maternal vitamin A status and to its ingestion by the mother during pregnancy. The aim of the present study was to assess the effect of maternal supplementation with retynil palmitate on the concentration of colostrum retinol under fasting and postprandial conditions. A total of 149 nursing mothers were recruited at the Januário Cicco Maternity School (Natal, Brazil) and allocated to two groups: Comparison (n = 69) and Test (n = 80). Blood and colostrum (in fasting and postprandial conditions) samples were collected up to 24hs after delivery. Serum retinol and colostrum levels were analyzed by high-performance liquid chromatography. The serum retinol level of 41.6 ± 12.7μg/dL (mean ± standard deviation) indicates adequate biochemical nutritional status. Colostrum retinol level was not influenced by serum retinol levels under any of the conditions established. In the colostrum, the retinol concentration in the unsupplemented test group was 67.3 ± 37.7 μg/dL under fasting and 80.3 ± 35.1 μg/dL under postprandial conditions (p<0.05), showing an increase of 19.3%. In the supplemented test group the values were 102.6 ± 57.3 μg/dL and 133.4 ± 78.3 μg/dL under fasting and postprandial, respectively (p<0.05), representing an increase of 30%. Considering that under fasting conditions most of the vitamin A transported to the milk originates in the retinol binding protein (RBP), the postprandial increase in colostrum retinol suggests a different transport mechanism of retinol to maternal milk from that performed by RBP. This situation becomes more evident under supplementation conditions.
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Mothers with good vitamin A nutritional status during gestation and lactation are better able to nourish and protect their infant with maternal milk. Our hypothesis is that women with more serum retinol have more retinol and secretory immunoglobulin A in colostrum. 190 healthy puerperal women from a Brazilian public maternity were recruited and divided according to the cutoff point for serum retinol (30 μg/dL). A number of the women was supplemented with 200000 UI (60 mg) of retinyl palmitate in the immediate postpartum. Serum and colostrum were collected on the 1st day postpartum and colostrum again on the following day. Retinol (serum and colostrum) was analyzed by HPLC and SIgA (colostrum) by turbidimetry. The mothers presented with adequate biochemical indicators of nutritional status, according to serum retinol (44.6 μg/dL). There were significant differences (p= 0.0017 and p= 0.043, respectively) in retinol and SIgA levels in the colostrum of mothers with serum retinol > 30 μg/dL and < 30 μg/dL. The concentration of SIgA in the colostrum of non-supplemented mothers on the 1st day postpartum was 822.6 mg/dL, decreasing after 24 hours to 343.7 mg/dL. Supplemented mothers showed levels of SIgA in colostrum of 498.9 mg/dL on the 2nd day postpartum (p= 0.00006). The colostrum of women with good vitamin A nutritional status had more retinol and SIgA. Additionally, maternal supplementation increases the levels of SIgA in colostrum. The higher levels of SIgA on the 1st day postpartum showed the importance of early breastfeeding, given that it provides considerable immunological benefits to newborn infants
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Vitamins A and E are essential nutrients in many biological processes, so that their adequate supply to the neonate is crucial. However, the bioavailability of vitamins may be limited by factors such as maternal nutritional status and the interaction between nutrients. This study aimed to investigate the effect of biochemical nutritional status of retinol and alpha-tocopherol levels in serum and colostrum. The study included 103 healthy puerperal women treated at the reference state maternity hospital (Natal-RN). Colostrum and serum samples were collected fasting in the immediate postpartum period and the analysis of retinol and alpha-tocopherol were determined by high-performance liquid chromatography. Specific cutoff points were adopted to characterize the biochemical status of vitamins A and E. For the total group of lactanting women the average concentration of retinol in serum (1.49 ± 0.4 μmol/L-1) and colostrum (2.18 ± 0.8 μmol/L-1), as well as alpha-tocopherol in serum (26.4 ± 8.0 μmol/L-1) and colostrum (26.1 ± 12.8 μmol/L-1), indicated adequate biochemical state. However, when evaluating the individual, was found a high prevalence of deficient serum (15%) and colostrum retinol (50%), and also alphatocopherol in serum (16%) and colostrum (61%). In women with serum retinol ≥ 1.05 μmol/L-1, found an inverse correlation between serum retinol and alpha-tocopherol in colostrum (p = 0.008, r = -0.28). This association was not observed in women with serum retinol <1.05 μmol/L-1. This situation demonstrates for the first time in humans that high physiological levels of serum retinol, without supplementation, can negatively influence the transfer of alpha-tocopherol in breast milk. Although the diagnosis of satisfactory nutritional status lactanting women showed high risk of subclinical deficiency of vitamins A and E from measurements made in the colostrum
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Dissertação de Mestrado apresentada ao Instituto Superior de Psicologia Aplicada para obtenção de grau de Mestre na especialidade de Psicologia Clínica.
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Tese (doutorado)—Universidade de Brasília, Faculdade de Comunicação, Programa de Pós-Graduação em Comunicação, 2016.
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No presente livro, apresenta-se uma síntese do trabalho de campo desenvolvido para Portugal, no âmbito do primeiro ano de atividade do projeto ERASMUS+ intitulado ISOLearn for HEI: Innovation and Social Learning for Higher Education Institutions (http://www.ISOLearn.net/), entre Outubro de 2014 e Outubro de 2016. No âmbito deste projeto europeu, coordenado pela Universidade Aberta, procurou-se fazer um levantamento das condições existentes para a inclusão de pessoas com deficiência visual e auditiva no Ensino Superior, em 4 países europeus: Portugal; Itália; Suécia e Eslovénia. Para tal foi desenvolvido um trabalho que consistiu na realização de entrevistas a Instituições de Ensino Superior (IES), e um conjunto de questionários online, a estudantes com este tipo de deficiências. Procurou-se fazer este levantamento enquadrado na legislação existente sobre este tema, e sobretudo na sensibilidade das principais partes interessadas (stakeholders) nesta temática, aferindo a sua perceção da realidade, procurando-se desta forma identificar eventuais desajustes entre o enquadramento legal e institucional vigente, e a prática das Instituições de Ensino Superior (IES) em cada um dos quatro países envolvidos. Considerando que os parceiros portugueses incluem entidades que se dedicam exclusivamente aos problemas da população em estudo, como é o caso da ACAPO – Associação dos Cegos e Amblíopes de Portugal; da APS – Associação Portuguesa de Surdos; e da Fundação Calouste Gulbenkian, que tem vindo a desenvolver trabalho na consagração dos Direitos Humanos e da inclusão de pessoas com deficiência; que por essa razão o trabalho de campo desenvolvido pelos parceiros portugueses foi extremamente rigoroso e em certa medida mais aprofundado, quer em termos metodológicos, quer em termos de amostra obtida – com entrevistas a 23 IES, e a participação de 49 estudantes, repartidos entre estudantes com deficiência visual e estudantes com deficiência auditiva – entendeu-se que seria importante autonomizar o trabalho sobre Portugal, dando-lhe a visibilidade que a edição eletrónica do presente livro pretende comportar. A presente edição pretende, pois, constituir apenas um pequeno pilar para o desenvolvimento do conhecimento sobre esta matéria em Portugal. É objetivo da equipa do projeto que, a partir da sua leitura se despertem consciências que levem à realização de mais trabalhos sobre esta temática, que possam permitir, não apenas uma maior sensibilização da sociedade portuguesa em geral sobre estas matérias, mas também, incitar todas as partes interessadas à ação, no sentido de uma maior inclusão das pessoas com deficiência visual e auditiva, no Ensino Superior. Naturalmente, tratando-se de um trabalho desenvolvido no âmbito de um projeto europeu, os objetivos definitivos vão para além das fronteiras nacionais, esperando-se que ele possa ajudar a própria Europa a desenvolver mais e melhores políticas para abordagem à temática da inclusão das pessoas com deficiência visual e auditiva no Ensino Superior no espaço europeu. Este deve ser considerado um trabalho em aberto; apenas um primeiro passo de uma longa e, espera-se, frutuosa caminhada, cujo destino será a inclusão das pessoas com deficiência visual e auditiva, no Ensino Superior.
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La vitamina D, una vitamina liposoluble que se encuentra en algunas fuentes de alimentos naturales, se sintetiza en la piel humana después de la exposición al sol. La insuficiencia y la deficiencia de vitamina D son muy frecuentes en el mundo, lo cual se cree que contribuye a una gran variedad de problemas de salud. La vitamina D durante mucho tiempo ha sido utilizada en combinación con el calcio para mejorar la salud ósea y reducir el riesgo de fracturas. Los suplementos de vitamina D se han relacionado con la prevención de la hipertensión arterial, cáncer y otras enfermedades. Investigaciones recientes también apuntan a una posible asociación entre la deficiencia de vitamina D y el dolor crónico.
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A transição da adolescência para a idade adulta é uma fase crucial do desenvolvimento de qualquer jovem, na qual são colocados vários desafios em relação ao seu futuro. Os adolescentes com deficiência enfrentam desafios adicionais: quando comparados com os seus pares, têm mais dificuldades em encontrar um percurso de vida adulta, por exemplo no emprego ou no prosseguimento de estudos. Por outro lado, a baixa participação social é um dos fatores que mais afeta a qualidade de vida. Percebendo-se que o processo de transição é multifacetado, as seis investigações apresentadas neste trabalho colocam o enfoque no próprio indivíduo, mas também em contextos importantes para a transição como a família, através da seguinte sequência de estudos: (1) dois estudos preliminares que caracterizam adolescentes e jovens com deficiência no que se refere a comportamentos ligados à saúde, estilos de vida e respetivas interacções com a satisfação com a vida; (2) dois estudos qualitativos que confirmam a problemática da transição e aferem os fatores críticos envolvidos, através das percepções de pais e jovens; e (3) dois estudos que fazem a análise do comportamento destes factores numa amostra de adolescentes e jovens com deficiência. Os resultados revelam um padrão de saúde e sintomático mais negativo nos jovens com deficiência quando comparados com os seus pares, e salientam a importância da relação com os outros significativos, em especial os pais. Os vários estudos mostram ainda que os contextos de vida devem proporcionar suportes adequados e atempados à transição e apelam para a importância do desenvolvimento de aptidões críticas, tais como a promoção de competências de autodeterminação, autoeficácia, e promoção da resiliência. Os resultados dos estudos são discutidos de forma integrada. São também feitas recomendações futuras desde a perspectiva individual até à das políticas e planeamento. Finalmente, é proposto um modelo do planeamento e desenvolvimento da transição que pretende ser orientador para todos os atores envolvidos no processo.
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This paper aims at studying how circular dance can afford to sight-disabled peoples movement and how they can learn to cope with the deep movement of relation, consciousness, appropriation and communion with the world. Inside circular dance, a cosmic metaphor, is inscribed the movement of the world, which tells and changes amorously the human history. In the works of Paulo Freire and Maurice Merleau-Ponty one can find the necessary support to discuss, as long as possible, movement and existence. Research-action is used as a methodological approach whose empirical center is placed on the Institute of Education and Rehabilitation of Blind, in Natal, which shelters eight sightdisabled adults. The research s data reveal that the practice of circular dance concurs to enlarge the movement of the research s subjects, to develop a more accurate perception of their selves and of their own capacities, as well as improve the relations Me/Others, Me/World, which require a context of differences. The study has revealed that the practice of dance develops a better perception of the limits and surpasses as a human condition and, in consequence, the discovery of one s own body and the other s body as a resource of lessons and representations of the self and of the world. It lets out the development of a new way of thinking and coping with discrimination surrounding the disabled persons. In movement, in circular dance, the barrier between sight disablement and vision loses force.
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Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Humanas, Departamento de Serviço Social, Programa de Pós-Graduação em Política Social, 2016.
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Haematological changes in ascorbic acid deficient fishes, observed by some authors, awoked the interest in the haematological response of pacu, Piaractus mesopotamicus. The present work studied the influence of different levels of ascorbic acid (vitamin C) in the ration on the haematological parameters of pacu. Fishes at the beginning of the experiment, presented average body weight 8.64±1.62g and 6.15±0.33cm standard length, were fed with diets containing 0. 50, 100 and 200mg ascorbic acid/kg dry ration. The present work was developed for a period of 24 weeks at the Fish Nutrition Laboratory of Aquaculture Center of Universidade Estadual Paulista (Unesp), Jaboticabal (São Paulo). The fishes were distributed by the completely randomized design in 20 aquaria of 100 liters capacity with six animals in each and five fold treatment. Condutivity, alcalinity. pH and oxigen were measured weekly and temperature daily. The blood colect was done in 60 anesthetized fishes with 50mg MS-222/1. The results suggests that 50mg ascorbic acid/kg dry ration improved the mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and decreased haematocril in unsupplemented fishes, although not differing significantly (P>0.05). The erytrocyte diameter indicated the presence of macrocytic cells in unsupplemented fishes (P<0.05) and the regression analysis showed too reduced neutrophils in unsupplemenled ones, 50mg ascorbic acid/kg dry ration supplementation was enough to development and haematological responses. Furthtermore, the optimum level to the growth is 139mg vitamin C/kg dry ration (P<0.05).