Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus

We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (similar to 60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present.

An investigation into the potential losses of fish on the filter plates at the Hodder Water Treatment Works Stocks Reservoir

Stocks Reservoir is situated amidst the Forest of Bowland in the upper reaches of the old river valley of the Hodder. The reservoir was built in 1927 for the Fylde Water Board who primarily supplied water to Blackpool. The objective of this study is to assess the degree and likelihood of fish ingress onto the fish plates at the present and proposed stocking densities. An additional aim is to evaluate the operational implications, and if necessary suggest methods of alleviating the problem. Three spheres of study have been undertaken to achieve these objectives, these being: 1. To selectively stock the reservoir and monitor the angling club catches in order to assess the total population, relating it to fish plate losses and proposed stocking densities. 2. To monitor the fish taken from the fish plates and assess the reasons for their ingress. 3. To study the draw off tower and fish plates, and suggest ways of ameliorating or halting the loss of fish and consequent operational problems.

One-dimensional potential for image-potential states on graphene

In the framework of dielectric theory, the static non-local self-energy of an electron near an ultra-thin polarizable layer has been calculated and applied to study binding energies of image-potential states near free-standing graphene. The corresponding series of eigenvalues and eigenfunctions have been obtained by numerically solving the one-dimensional Schrodinger equation. The imagepotential state wave functions accumulate most of their probability outside the slab. We find that the random phase approximation (RPA) for the nonlocal dielectric function yields a superior description for the potential inside the slab, but a simple Fermi-Thomas theory can be used to get a reasonable quasi-analytical approximation to the full RPA result that can be computed very economically. Binding energies of the image-potential states follow a pattern close to the Rydberg series for a perfect metal with the addition of intermediate states due to the added symmetry of the potential. The formalism only requires a minimal set of free parameters: the slab width and the electronic density. The theoretical calculations are compared with experimental results for the work function and image-potential states obtained by two-photon photoemission.

Feeding ecology of juvenile Pacific salmon (Oncorhynchus spp.) in a northeast Pacific fjord: diet, availability of zooplankton, selectivity for prey, and potential competition for prey resources

We investigated the feeding ecology of juvenile salmon during the critical early life-history stage of transition from shallow to deep marine waters by sampling two stations (190 m and 60 m deep) in a northeast Pacific fjord (Dabob Bay, WA) between May 1985 and October 1987. Four species of Pacific salmon—Oncorhynchus keta (chum) , O. tshawytscha (Chinook), O. gorbuscha (pink), and O. kisutch (coho)—were examined for stomach contents. Diets of these fishes varied temporally, spatially, and between species, but were dominated by insects, euphausiids, and decapod larvae. Zooplankton assemblages and dry weights differed between stations, and less so between years. Salmon often demonstrated strongly positive or negative selection for specific prey types: copepods were far more abundant in the zooplankton than in the diet, whereas Insecta, Araneae, Cephalapoda, Teleostei, and Ctenophora were more abundant in the diet than in the plankton. Overall diet overlap was highest for Chinook and coho salmon (mean=77.9%)—species that seldom were found together. Chum and Chinook salmon were found together the most frequently, but diet overlap was lower (38.8%) and zooplankton biomass was not correlated with their gut fullness (%body weight). Thus, despite occasional occurrences of significant diet overlap between salmon species, our results indicate that interspecific competition among juvenile salmon does not occur in Dabob Bay.

Spatial and temporal distribution and the potential for estuarine interactions between wild and hatchery chum salmon (Oncorhynchus keta) in Taku Inlet, Alaska

We investigated estuarine spatial and temporal overlap of wild and marked hatchery chum salmon (Oncorhynchus keta) fry; the latter included two distinct size groups released near the Taku River estuary (Taku Inlet) in Southeast Alaska (early May releases of ~ 1.9 g and late May releases of ~ 3.9 g wet weight). Our objectives were to compare abundance, body size, and condition of wild chum salmon fry and hatchery chum salmon fry raised under early and late rearing strategies in different habitats of Taku Inlet and to document environmental factors that could potentially explain the distribution, size, and abundance of these chum salmon fr y. We used a sampling design stratified into inner and outer inlet and neritic and littoral habitats. Hatchery fry were rare in the inner estuary in both years but outnumbered wild fry 20:1 in the outer estuary. Hatchery fry were significantly larger than wild fry in both littoral and neritic samples. Abundances of wild and hatchery fry were positively correlated in the outer inlet, indicating the formation of mixed schools of hatchery and wild fry. Spatial and temporal overlap was greatest between wild and early hatchery fry in the outer inlet in both habitats. The early hatchery release coincided with peak abundances of wild fry in the outer inlet, and the distribution of wild and early hatchery fry overlapped for about three weeks. Our results demonstrate that the timing of release of hatchery fry may affect interactions with wild fry.

Reflex impairment as a measure of vitality and survival potential of Atlantic cod (Gadus morhua)

A ssur ing the v itality and survival potential of live-caught Atlantic cod (Gadus morhua) is important for improving the sorting of fish before net penning operations designed to hold fish for growth and later market. When Atlantic cod are captured by Danish seine, the most commonly used fishing gear for live-caught fish, they undergo stressors such as forced swimming, net abrasion, and air exposure. Laboratory experiments (at an air temperature of 9°C and water temperature of 8°C) were conducted with the aim of constructing a RAMP (reflex action mortality predictor) curve for prediction of vitality and survival potential in Atlantic cod captured in Danish seines, by varying the levels of these stressors. Atlantic cod exposed to increased duration in air (5–20 min) showed increased reflex impairment and mortality, with 75% mortality at 10 minutes of air exposure. Forced swimming in combination with net abrasion and air exposure did not increase reflex impairment or mortality above that associated with air exposure alone. The Atlantic cod RAMP curves indicated that fish with reflex impairment less than 50% would not show mortality and would likely recover from capture stress.

Relationship between abundance of juvenile rockfishes (Sebastes spp.) and environmental variables documented off northern California and potential mechanisms for the covariation

We estimated annual abundance of juvenile blue (Sebastes mystinus), yellowtail (S. f lavidus), and black (S. melanops) rockfish off northern California over 21 years and evaluated the relationship of abundance to oceanographic variables (sea level anomaly, nearshore temperature, and offshore Ekman transport). Although mean annual abundance was highly variable (0.01−181 fish/minute), trends were similar for the three species. Sea level anomaly and nearshore temperature had the strongest relationship with interannual variation in rockfish abundance, and offshore Ekman transport did not correlate with abundance. Oceanographic events occurring in February and March (i.e., during the larval stage) had the strongest relationship with juvenile abundance, which indicates that year-class strength is determined during the larval stage. Also of note, the annual abundance of juvenile yellowtail rockfish was positively correlated with year-class strength of adult yellowtail rockfish; this finding would indicate the importance of studying juvenile abundance surveys for management purposes.

The potential use of time-area closures to reduce catches of bigeye tuna (Thunnus obesus) in the purse-seine fishery of the eastern Pacific Ocean

Skipjack (Katsuwonus pelamis), yellowfin (Thunnus albacares), and bigeye (Thunnus obesus) tunas are caught by purse-seine vessels in the eastern Pacific Ocean (EPO). Although there is no evidence to indicate that current levels of fishing-induced mortality will affect the sustainability of skipjack or yellowfin tunas, fishing mortality on juvenile (younger than 5 years of age) bigeye tuna has increased, and overall fishing mortality is greater than that necessary to produce the maximum sustainable yield of this species. We investigated whether time-area closures have the potential to reduce purse-seine bigeye catches without significantly reducing skipjack catches. Using catch and effort data for 1995–2002, we identified regions where the ratio of bigeye to skipjack tuna catches was high and applied simple closed-area models to investigate the possible benefits of time-area closures. We estimated that the most optimistic and operationally feasible 3-month closures, covering the equatorial region of the EPO during the third quarter of the year, could reduce bigeye catches by 11.5%, while reducing skipjack tuna catches by 4.3%. Because this level of bigeye tuna catch reduction is insufficient to address sustainability concerns, and larger and longer closures would reduce catches of this species signficantly, we recommend that future research be directed toward gear technology solutions because these have been successful in many other fisheries. In particular, because over 50% of purse-seine catches of bigeye tuna are taken in sets in which bigeye tuna are the dominant species, methods to allow the determination of the species composition of aggregations around floating objects may be important.