Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus
Data(s) |
22/01/2016
22/01/2016
16/03/2015
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Resumo |
We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (similar to 60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present. |
Identificador |
PLoS ONE 10(3) : (2015) // e0119401 1932-6203 http://hdl.handle.net/10810/16798 10.1371/journal.pone.0119401 |
Idioma(s) |
eng |
Publicador |
Public Library Science |
Relação |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119401 |
Direitos |
© 2015 Canduela et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited info:eu-repo/semantics/openAccess |
Palavras-Chave | #long term depression #TRPV1 channels #immunohistochemical localization #endocannabinoid system #cannabinoid receptor #synaptic plasticity #brain #mice #expression #hypocampus |
Tipo |
info:eu-repo/semantics/article |