Thin films prepared from tungstate glass matrix

Vitreous samples containing high concentrations of WO3 (above 40% M) have been used as a target to prepare thin films. Such films were deposited using the electron beam evaporation method onto soda-lime glass substrates. These films were characterized by X-ray diffraction (XRD), perfilometry, X-ray energy dispersion spectroscopy (EDS), M-Lines and UV-vis absorption spectroscopy. In this work, experimental parameters were established to obtain stable thin films showing a chemical composition close to the glass precursor composition and with a high concentration of WO3. These amorphous thin films of about 4 mu m in thickness exhibit a deep blue coloration but they can be bleached by thermal treatment near the glass transition temperature. Such bleached films show several guided modes in the visible region and have a high refractive index. Controlled crystallization was realized and thus it was possible to obtain WO3 microcrystals in the amorphous phase. (C) 2007 Elsevier B.V. All rights reserved.

Cloning, expression, purification and characterization of recombinant glutathione-S-transferase from Xylella fastidiosa

Xylella fastidiosa is an important pathogen bacterium transmitted by xylem-feedings leafhoppers that colonizes the xylem of plants and causes diseases on several important crops including citrus variegated chlorosis (CVC) in orange and lime trees. Glutathione-S-transferases (GST) form a group of multifunctional isoenzymes that catalyzes both glutathione (GSH)-dependent conjugation and reduction reactions involved in the cellular detoxification of xenobiotic and endobiotic compounds. GSTs are the major detoxification enzymes found in the intracellular space and mainly in the cytosol from prokaryotes to mammals, and may be involved in the regulation of stress-activated signals by suppressing apoptosis signal-regulating kinase 1. In this study, we describe the cloning of the glutathione-S-transferase from X. fastidiosa into pET-28a(+) vector, its expression in Escherichia coli, purification and initial structural characterization. The purification of recombinant xfGST (rxfGST) to near homogeneity was achieved using affinity chromatography and size-exclusion chromatography (SEC). SEC demonstrated that rxfGST is a homodimer in solution. The secondary and tertiary structures of recombinant protein were analyzed by circular dichroism and fluorescence spectroscopy, respectively. The enzyme was assayed for activity and the results taken together indicated that rxfGST is a stable molecule, correctly folded, and highly active. Several members of the GST family have been extensively studied. However, xfGST is part of a less-studied subfamily which yet has not been structurally and biochemically characterized. In addition, these studies should provide a useful basis for future studies and biotechnological approaches of rxfGST. (C) 2008 Elsevier Inc. All rights reserved.

TNF-alpha polymorphisms are associated with obsessive-compulsive disorder

Introduction: Several lines of evidence support an immunologic involvement in obsessive-compulsive disorder (OCD): the increased prevalence of OCD in patients with rheumatic fever (RF), and the aggregation of obsessive-compulsive spectrum disorders among relatives of RF probands. Tumor necrosis factor alpha is a proinflammatory cytokine involved in RF and other autoimmune diseases. Polymorphisms in the promoter region of the TNFA gene have been associated with RE Given the association between OCD and RF, the goal of the present study was to investigate a possible association between polymorphisms within the promoter region of TNFA and OCD. Materials and methods: Two polymorphisms were investigated: -308 G/A and -238 G/A. The allelic and genotypic frequencies of these polymorphisms were examined in 111 patients who fulfilled DSM-IV criteria for OCD and compared with the frequencies in 250 controls. Results: Significant associations were observed between both polymorphisms and OCD. For -238 G/A, an association between the A allele and OCD was observed (X-2 = 12.05, p = 0.0005). A significant association was also observed between the A allele of the -308 G/A polymorphism and OCD (X-2 = 7.09, p = 0.007). Finally, a haplotype consisting of genotypes of these two markers was also examined. Significant association was observed for the A-A haplotype (p = 0.0099 after correcting for multiple testing). Discussion: There is association between the -308 G/A and -238 G/A TNFA polymorphisms and OCD in our Brazilian sample. However, these results need to be replicated in larger samples collected from different populations. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

Proteomic analysis reveals suppression of bark chitinases and proteinase inhibitors in citrus plants affected by the citrus sudden death disease

Citrus sudden death (CSD) is a disease of unknown etiology that greatly affects sweet oranges grafted on Rangpur lime rootstock, the most important rootstock in Brazilian citriculture. We performed a proteomic analysis to generate information related to this plant pathogen interaction. Protein profiles from healthy, CSD-affected and CSD-tolerant stem barks, were generated using two-dimensional gel electrophoresis. The protein spots were well distributed over a pI range of 3.26 to 9.97 and a molecular weight (MW) range from 7.1 to 120 kDa. The patterns of expressed proteins on 2-DE gels made it possible to distinguish healthy barks from CSD-affected barks. Protein spots with MW around 30 kDa and pI values ranging from 4.5 to 5.2 were down-regulated in the CSD-affected rootstock bark. This set of protein spots was identified as chitinases. Another set of proteins, ranging in pI from 6.1 to 9.6 with an MW of about 20 kDa, were also suppressed in CSD-affected rootstock bark; these were identified as miraculin-like proteins, potential trypsin inhibitors. Downregulation of chitinases and proteinase inhibitors in CSD-affected plants is relevant since chitinases are well-known pathogenesis-related protein, and their activity against plant pathogens is largely accepted.

The use of polyacrylamides (PAMs) for removing natural organic matter (NOM) from Paraiba do Sul River (Brazil)

This work assesses the efficiency of polyacrylamides for natural organic matter (NOM) removal from Paraiba do Sul River (Brazil) raw water for drinking purposes. Jar tests were performed following an experimental design protocol. Three kinds of polyacrylamides (anionic, cationic, and non-ionic) at 0.2 mg L(-1) were tested. After coagulation, turbidity, DOC, UVA(254) and SCAN (UV-absorbing material) were determined. Color and pH were also measured. It was found that polyacrylamides did not reduce the amounts of alum and lime needed in the process and that the amount of alum alone for removing UV-absorbing organic matter is significantly higher. Efficiency of the coagulation process decreased as follows: non-ionic -> cationic -> anionic -> no polyacrylamide. Removal efficiencies for the best case were: 100%, 90%, 83%, and 68% for turbidity, DOC, UVA(254), and SCAN, respectively.

Nuclear magnetic resonance characterization of metabolite disorder in orange trees caused by citrus sudden death disease

Citrus sudden death (CSD) is a new disease of sweet orange and mandarin trees grafted on Rangpur lime and Citrus volkameriana rootstocks. It was first seen in Brazil in 1999, and has since been detected in more than four million trees. The CSD causal agent is unknown and the current hypothesis involves a virus similar to Citrus tristeza virus or a new virus named Citrus sudden death-associated virus. CSD symptoms include generalized foliar discoloration, defoliation and root death, and, in most cases, it can cause tree death. One of the unique characteristics of CSD disease is the presence of a yellow stain in the rootstock bark near the bud union. This region also undergoes profound anatomical changes. In this study, we analyse the metabolic disorder caused by CSD in the bark of sweet orange grafted on Rangpur lime by nuclear magnetic resonance (NMR) spectroscopy and imaging. The imaging results show the presence of a large amount of non-functional phloem in the rootstock bark of affected plants. The spectroscopic analysis shows a high content of triacylglyceride and sucrose, which may be related to phloem blockage close to the bud union. We also propose that, without knowing the causal CSD agent, the determination of oil content in rootstock bark by low-resolution NMR can be used as a complementary method for CSD diagnosis, screening about 300 samples per hour.

La carne recordada : un análisis de la atemporalidad mítica y la subversión religiosa en Pedro Páramo

The aim of the present study is to analyze the representation of the mythic timelessness and the religious subversion in the novel Pedro Páramo (1953) by the Mexican writer Juan Rulfo. The novel is analyzed from a narratological perspective based on the concept of focalization proposed by the French theoretician Gérard Genette. According to Genette it is possible to identify different levels of focalization depending on the position of the narrative voices.The key question in this investigation is if any salvation is possible in the universe of Pedro Páramo and how the mythic timelessness and the religious subversion are represented in the textual intentionality through the narrative voices. The main conclusion is that there is no possible salvation in the fictional universe of Pedro Páramo. The collective sin is so vast that the intermediation between God and the inhabitants of Comala does not work anymore. Thus, the only possible ”salvation” consists in the separation of the soul from the body the people of Comala experience after death. In Pedro Páramo the dead body is liberated from remorses, but - contrary to the Catholic concepts - becomes a conscious materia sentenced to remember eternally.Keywords: Juan Rulfo, Pedro Páramo, narratological analysis, Gérard Genette, textual intentionality, focalization, mythic timelessness, religious subversion, narrative voices.

Actitudes hacia la lengua náhuatl : Un estudio sociolingüístico con jóvenes de la Ciudad deMéxico.

Nahuatl is one of the indigenous languages in Mexico that is at risk of falling out of use. The research in the last years have showed that this occurs due to the low amount of Nahuatl speakers and the social stigma and discrimination towards the indigenous peoples, among other factors.The studies of language attitudes are relevant when making attempts to revitalize a language. As Mexico City authorities have considered to implement a revitalization project, we wanted to approach the real situation of Nahuatl in that city. Our purpose was to measure the attitudes of young people towards this language. By using a direct method –a questionnaire- we asked two groups of students if they agreed or disagreed with some items about Nahuatl and its speakers. One group had contact with Nahuatl speakers and but not the other one. We compared the results and they showed that the students who have contact with Nahuatl have more positive attitudes than those who don’t have any contact with it. This study also revealed that the reason why the first group showed positive attitudes was solidarity with the Nahuatl speakers. However, the both groups considered that speaking or learning Nahuatl cannot contribute to raise their status in the Mexican society.

IT­-Forensisk undersökning av flyktigt minne : På Linux och Android enheter

Att kunna gör en effektiv undersökning av det flyktiga minnet är något som blir viktigare ochviktigare i IT-forensiska utredningar. Dels under Linux och Windows baserade PC installationermen också för mobila enheter i form av Android och enheter baserade andra mobila opperativsy-stem.Android använder sig av en modifierad Linux-kärna var modifikationer är för att anpassa kärnantill de speciella krav som gäller för ett mobilt operativsystem. Dessa modifikationer innefattardels meddelandehantering mellan processer men även ändringar till hur internminnet hanteras ochövervakas.Då dessa två kärnor är så pass nära besläktade kan samma grundläggande principer användas föratt dumpa och undersöka minne. Dumpningen sker via en kärn-modul vilket i den här rapportenutgörs av en programvara vid namn LiME vilken kan hantera bägge kärnorna.Analys av minnet kräver att verktygen som används har en förståelse för minneslayouten i fråga.Beroende på vilken metod verktyget använder så kan det även behövas information om olika sym-boler. Verktyget som används i det här examensarbetet heter Volatility och klarar på papperet avatt extrahera all den information som behövs för att kunna göra en korrekt undersökning.Arbetet avsåg att vidareutveckla existerande metoder för analys av det flyktiga minnet på Linux-baserade maskiner (PC) och inbyggda system(Android). Problem uppstod då undersökning avflyktigt minne på Android och satta mål kunde inte uppnås fullt ut. Det visade sig att minnesanalysriktat emot PC-plattformen är både enklare och smidigare än vad det är mot Android.

Kostnader och risker med internationell verksamhet i Mexiko

Detta examensarbetes syfte är att identifiera vilka faktorer som påverkar kostnader och risker som kan finnas vid internationellt företagande i Mexiko utifrån ett perspektiv där företagande samt företagsomgivningar vanligtvis är stöttande och välfungerande. En litteraturstudie utfördes på Internationella verksamheter (International Business), och skapade intervjuunderlaget som användes för att intervjua företagare i Mexiko med erfarenheter av både internationell verksamhet samt USA/EU, och enbart erfarenheter från företagande inom landet. Resultat visar att det finns sex stycken samlingsområden som påverkar kostnader och risker; Politiska faktorer som innebär att drastiska förändringar orsakas i ett lands affärsomgivning, och som negativt påverkar ett företags vinster eller andra mål; Ekonomiska faktorer som innebär att det kostar mer att ha verksamhet på grund av brister inom infrastruktur och stödjande verksamheter; Den ekonomiska utvecklingen; Det finns utbredd korruption hos Mexikanska myndigheter på både lokal- och statsnivå; Ledningsprocesser och metoder kan behöva anpassas enligt kulturellt bestämda arbetsrelaterade värderingar; och Internationella verksamheter bör anställa lokala individer till hjälp att utföra affärer inom en specifik kultur.

Estabilização pozolânica de cinzas volante e pesada com cal dolomítica hidratada e areia, curadas pelos processos de autoclavagem e câmara a temperatura constante

Este trabalho tem como objetivo verificar o comportamento mecânico e físico de pozolanas artifíciais estabilizadas química e granulometricamente, curadas por dois processos de cura denominados de : autoclave(ATC), que utiliza temperaturas na faixa de 149 a 188°C e câmara à temperatura constante(CTC) que utiliza uma temperatura de ± 21°C. Também fez-se análises estatísticas com a finalidade de se determinar o efeito da energia de moldagem, temperatura e tempo de cura sobre os resultados dos ensaios, para cada processo de cura, além de se determinar modelos matemáticos para previsão de resultados de resistência através de regressões múltiplas e simples. As pozolanas artificiais empregadas foram as cinzas volante e pesada da Usina de Candiota, as quais foram estabilizadas com cal dolomítica hidratada e areia do Rio Guaíba. Os ensaios de resistência à compressão simples , absorção e perda de massa basearam-se nas normas da ABNT e DNER e para os estudos de análise estatística, fez-se anteriormente aos ensaios, dois planejamentos experimentais denominados de Split-Splot e Quadrado Latino, que foram utilizados nos processos de autoclavagem e câmara à temperatura constante, representativamente. Os Corpos-de-Prova curados na câmara à temperatura constante, até os 28 dias de cura, apresentaram resultados de resistências inferiores aqueles curados pelo processo de autoclave. Aos 60 dias de cura suas resistências ficaram na faixa dos valores de Corpos-de-Provas curados pela autoclave nas temperaturas de 149 a 188°C, excessão feita na mistura utilizando areia, onde em todos os períodos de cura estudados,os valores de resistência dos Corpos-de-Prova curados pelo câmara à temperatura constante foram inferiores. A proporção da quantidade de cal e cinza na mistura, bem como o valor da superfície específica da cinza influenciam nos resultados de ensaios, independentemente da variação dos fatores principais. Em termos de análise estatística verificou-se que a energia de moldagem e o tempo de cura são os fatores que apresentam os maiores efeitos sobre os resultados da resistência, para os processos de cura ATC e CTC, respectivamente.

Estudo do comportamento de um solo mole tratado com cal, visando seu uso em fundações superficiais

Neste trabalho foi analisada a melhoria nas características de um solo mole quando tratado com cal, bem como a viabilidade técnica de se utilizar este novo material como uma camada suporte de fundações superficiais. O solo estudado classifica-se pedologicamente como Gley Húmico e a jazida localiza-se no município de Canoas/RS, às margens da BR 386. O trabalho teve as seguintes finalidades: realizar um estudo da influência dos diferentes teores de cal sobre as características tensão x deformação do solo tratado; verificar o ganho de resistência com o tempo de cura; modelar o comportamerito tensão x deformação do material tratado; realizar simulações numéricas, através do Método dos Elementos Finitos, do comportamento carga x recalque de fundações continuas flexíveis assentes sobre o novo material. Adotou-se o teor ótimo de cal (obtido pelo método de Eades & Grim, 1966) de 9% e dois valores inferiores de 7% e 5%. Realizaram-se os seguintes ensaios sobre o solo natural e as misturas de solo-cal: limites de Atterberg, compactação, granulometria, difratograma de raio X, permeabilidade (triaxial) e ensaios triaxiais adensados não drenados(CIU). Todos os ensaios foram realizados para três tempos de cura (7, 28 e 90 dias) e os corpos de prova foram curados em câmara úmida. Para modelar o comportamento tensão x deformação do solo melhorado, adotou-se o Modelo Hiperbólico e para o solo natural o Modelo Cam-Clay Modificado. O Modelo Hiperbólico foi implementado no software CRISPSO, desenvolvido na Universidade de Cambridge, Inglaterra. O software foi utilizado em um estudo paramétrico para determinar a influência do processo de estabilização no comportamento carga x recalque de fundações superficiais. Dos resultados obtidos, concluiu-se: que o método de Eades & Grim (1966) não mostrou-se adequado para determinação do teor ótimo de cal; houve, de maneira geral, melhora nas características físicas com o tratamento com cal; não houve ganho de resistência com o tempo de cura; o modelo hiperbólico representou bem o comportamento das misturas de solo cal e a colocação de uma camada de solo tratado apresenta melhoras no comportamento carga x recalque de fundações superficiais contínuas flexíveis.