Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus

Argonaute 2 gene plays a pivotal role in RNAi in many species. Herein is the first report of the cloning and characterization of Argonaute 2 gene in fish. The full-length cDNA of Gobiocypris rarus Argonaute 2 (GrAgo2) consisted of 3073 nucleotides encoding 869 amino acid residues with a calculated molecular weight of 98.499 kDa and an estimated isoelectric point of 9.18. Analysis of the deduced amino acid sequence showed the presence of two signature domains, PAZ and Piwi. RT-PCR analysis indicated that GrAgo2 mRNA expression could be detected in widespread tissues. After infection with grass carp reovirus, GrAgo2 expression was up-regulated from 12 h post-injection (p < 0.05) and returned to control levels at 48 h post-injection (p > 0.05). These data imply that GrAgo2 is involved in antiviral defense in rare minnow. (C) 2008 Published by Elsevier Ltd.

Light and scanning electron microscopic study of Balantidium ctenopharyngodoni Chen, 1955 (Class : Litostomatea) from China

Redescription of Balantidium ctenopharyngodoni "Chen (Acta Hydrobiol Sin 1:123-164, 1955)", collected from the hindgut of grass carp (Ctenopharyngodon idella), especially the segment of 6-10 cm upstream from the anus, from Honghu Lake, Hubei Province, central China in November 2005, is presented in this paper to complete Chen's description at both light and scanning electron microscopic levels. Some revisions were done: the vestibulum is fairly symmetrical, with compactly arranged cilia rather than assembled membrane bordering on the left vestibular side; four contractile vacuoles actually exist in the latter body, three of which surround the posterior portion of the macronucleus, whereas the fourth lies antero-left to it. Somatic monokinetids were compared among the species of genus Balantidium. The cysts were described, and possible infection routes of B. ctenopharyngodoni were also discussed.

A non-linear perturbation model considering catchment wetness and its application in river flow forecasting

A non-linear perturbation model for river flow forecasting is developed, based on consideration of catchment wetness using an antecedent precipitation index (API). Catchment seasonality, of the form accounted for in the linear perturbation model (the LPM), and non-linear behaviour both in the runoff generation mechanism and in the flow routing processes are represented by a constrained nan-linear model, the NLPM-API. A total of ten catchments, across a range of climatic conditions and catchment area magnitudes, located in China and in other countries, were selected for testing daily rainfall-runoff forecasting with this model. It was found that the NLPM-API model was significantly more efficient than the original linear perturbation model (the LPM). However, restric tion of explicit nan-linearity to the runoff generation process, in the simpler LPM-API form of the model, did not produce a significantly lower value of the efficiency in flood forecasting, in terms of the model efficiency index R-2. (C) 1997 Elsevier Science B.V.

Study on text-dependent speaker recognition based on Biomimetic Pattern Recognition

We studied the application of Biomimetic Pattern Recognition to speaker recognition. A speaker recognition neural network using network matching degree as criterion is proposed. It has been used in the system of text-dependent speaker recognition. Experimental results show that good effect could be obtained even with lesser samples. Furthermore, the misrecognition caused by untrained speakers occurring in testing could be controlled effectively. In addition, the basic idea "cognition" of Biomimetic Pattern Recognition results in no requirement of retraining the old system for enrolling new speakers.

A 2x2 optical switch based on plasma dispersion effect in silicon-on-insulator

Based on Mach-Zehnder interferometer (MZI) structure, a 2 x 2 optical switch is fabricated on SOI wafer. Modulation of the refractive index of MZI arms is achieved through free carriers plasma dispersion effect of silicon. The device presents an insertion loss as low as 3.44 dB and a response time as small as 300 ns. The crosstalk and extinction ratio are -15.54 and 14.9 dB, respectively. Detailed analysis and explanation of the operating behaviors are also presented. (C) 2005 Elsevier B.V. All rights reserved.

Optical properties of self-assembled ternary In(GA/Al)As quantum dots on (100) and (N 1 1)B InP substrates

In this paper, we investigated the self-assembled quantum dots formed on (100) and (N11)B (N = 2, 3, 4, 5) InP substrates by molecular beam epitaxy (MBE). Two kinds of ternary QDs (In0.9Ga0.1As and In0.9Al0.1As QDs) are grown on the above substrates; Transmission electron microscopy (TEM) and photoluminescence (PL) results confirm QDs formation for all samples. The PL spectra reveal obvious differences in integral luminescence, peak position, full-width at half-maximum and peak shape between different oriented surfaces. Highest PL integral intensity is observed from QDs on (411)B surfaces, which shows a potential for improving the optical properties of QDs by using high-index surface. (C) 2000 Elsevier Science B.V. All rights reserved.

Multi channel micro neural probe fabricated with SOI

Extracellular neural recording requires neural probes having more recording sites as well as limited volumes. With its mechanical characteristic and abundant process method, Silicon is a kind of material fit for producing neural probe. Silicon on insulator (SOI) is adopted in this paper to fabricate neural probes. The uniformity and manufacturability are improved. The fabricating process and testing results of a series of Multi channel micro neural probes were reported. The thickness of the probe is 15 mu m-30 mu m. The typical impedance characteristics of the record sites are around 2M Omega at 1k Hz. The performance of the neural probe in-vivo was tested on anesthetic rat. The recorded neural spike was typically around 140 mu V. Spike recorded from individual site could exceed 700 mu V. The average signal noise ratio was 7 or more.

Study on text-dependent speaker recognition based on Biomimetic Pattern Recognition

We studied the application of Biomimetic Pattern Recognition to speaker recognition. A speaker recognition neural network using network matching degree as criterion is proposed. It has been used in the system of text-dependent speaker recognition. Experimental results show that good effect could be obtained even with lesser samples. Furthermore, the misrecognition caused by untrained speakers occurring in testing could be controlled effectively. In addition, the basic idea "cognition" of Biomimetic Pattern Recognition results in no requirement of retraining the old system for enrolling new speakers.

Performance of an embedded optical vector matrix multiplication processor architecture

An embedded architecture of optical vector matrix multiplier (OVMM) is presented. The embedded architecture is aimed at optimising the data flow of vector matrix multiplier (VMM) to promote its performance. Data dependence is discussed when the OVMM is connected to a cluster system. A simulator is built to analyse the performance according to the architecture. According to the simulation, Amdahl's law is used to analyse the hybrid opto-electronic system. It is found that the electronic part and its interaction with optical part form the bottleneck of system.

一种改进的BMH模式匹配算法

分析了目前网络上最流行的BM算法及其改进算法BMH,在此基础上提出了BMH算法的改进算法BMH2。考虑了模式串自身的特征,在原有移动距离数组的基础上增加一个新的移动数组,从而充分利用模式串特征进行更大距离的移动,使算法获得更高的效率。实验证明,改进后的算法能够增加"坏字符"方法的右移量,有效地提高匹配速率。

一种多业务宽带接入交换机的设计与实现

在接入网中提供多业务可以有效地降低运营维护成本和网络铺设费用,同时提高网络利用率.采用组播技术和具有多项服务质量(QoS)选择的多层以太网交换机,可以实现能提供QoS保证的多业务宽带接入网.该文介绍了一种多业务宽带接入交换机的设计与实现,按功能模块分述并讨论了其中的难点和要点.

reducing symmetries to generate easier sat instances

Finding countermodels is an effective way of disproving false conjectures. In first-order predicate logic, model finding is an undecidable problem. But if a finite model exists, it can be found by exhaustive search. The finite model generation problem in the first-order logic can also be translated to the satisfiability problem in the propositional logic. But a direct translation may not be very efficient. This paper discusses how to take the symmetries into account so as to make the resulting problem easier. A static method for adding constraints is presented, which can be thought of as an approximation of the least number heuristic (LNH). Also described is a dynamic method, which asks a model searcher like SEM to generate a set of partial models, and then gives each partial model to a propositional prover. The two methods are analyzed, and compared with each other.

基于OSGi的服务协作总线的设计与实现

网络技术的快速发展和多变的业务需求使得软件功能日益复杂、规模日益庞大。面向服务架构作为一种新的计算范型，利用服务作为基本的构造单元，支持异构环境下分布式应用的快速、低成本开发。但是现有的面向服务架构实现方案在服务动态部署、动态生命周期管理，以及模块化方面均存在不足。OSGi（Open Service Gateway Initiative）提供了一种面向服务、基于组件的软件框架，易于构建模块化、动态可扩展的软件系统，在服务动态部署、生命周期管理，以及模块化方面具有优势。 论文首先分析了面向服务架构和OSGi技术的特点，以及相互之间的互补因素，在此基础上建立了一个服务协作总线概念架构，支持服务的动态部署、动态生命周期管理和透明协作。在该概念架构的基础上，设计了一种服务描述模型，以此为基础实现透明的OSGi服务发布与发现；扩展了OSGi服务部署和访问机制，实现分布透明的OSGi服务访问；通过声明式远程服务组件模型，实现了服务动态生命周期管理。在这些基础上，论文给出了服务协作总线系统OnceSCB的设计与实现。并通过应用案例和实验对OnceSCB的功能和非功能方面进行了评估。该服务协作总线实现远程服务的透明化访问以及远程服务与本地OSGi服务统一管理，提供透明的服务协作，形成一个基于服务协作的动态服务计算生态环境，为服务计算环境下服务开发、部署、管理提供支持。

Real-Time SQL的设计与实现

实时数据库的结构化查询语言RTSQL(Real-Time SQL)是实时数据库研究的一项重要内容.论文详细论述了RTSQL的一种设计方法,即扩展SQL92标准以支持实时数据库的要求,构建RTSQL语言的方法.文章还介绍了RTSQL在Agilor实时数据库系统中的实现方式.在文章最后给出了RTSQL进一步研究的思路和建议。