994 resultados para germination experiment


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The spore germination in Thelypteris dentata was studied and the optimum temperature of 25 C was determined by the maximum percentage germination and the highest germination index. The necessity of continuous white light irradiation and reversion of spore germination by subsequent far-red light irradiation indicate the involvement of phytochrome in the control of the process. The dependence of induction of spore germination to the continuous white light low irradiance confirms the phytochrome control through the low fluence response.

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T. micrantha (L.) Blume (Ulmaceae), a common pioneer tree species in Brazil, is used in the restoration of degraded areas. The fruits are fleshy and indehiscent, with only one water impermeable seed. During the fruiting period, fruits of different colours are found at the same time on the same branch. This research aimed to correlate fruit colour with other physical indicators of seed maturity and to Verify the effect of temperature regime on seed germination. Collected fruits were separated in to green, green-red and red colour and for each of these maturation stages, size, moisture content and dry matter of both fruits and seeds were determined. Seeds were scarified with sulphuric acid and submitted to a germination test conducted at constant (20 degreesC, 30 degreesC and 40 degreesC) and alternating (20-30 degreesC, 30-40 degreesC and 20-40 degreesC) temperatures for 15 weeks. Seed germination percentage and speed were analysed after five weeks and the fmal percentage of germinated and Viable seeds after 15 weeks. Seed maturity is attained when the fruits are green-red. At this stage, moisture content was about 64% for fruits and 10% for seeds. Alternating temperature was required for seed germination and 20-30 degreesC was the best option. Most seeds had germinated after five weeks, providing mature seeds, acid scarification and alternating temperature were used.

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The Syagrus romanzoffiana is a specie of the Arecaccae family, native of Brazil, frequently used in landscape architecture. Its propagation is by seeds, however, there is little information in the literature about seed germination of this ornamental palm. The objective of this work was to study the effects of temperatures on seed germination of Syagrus romanzoffliana. The experimental design used was entirely randomized, with six temperatures (20 degrees C, 25 degrees C, 30 degrees C and 35 degrees C, constant and 20-30 degrees C and 25-35 degrees C alternated), photoperiod of 12 hours, and five replications of 20 seeds each. The seeds were placed in plastic boxes with sand, counting daily germination until 43(th) day. The percentage of seed germination (43(th) day) and the speed germination index (SGI) were evaluated. The conclusion was that there was no germination at a constant temperature of 20 degrees C and in the alternated temperature of 20-30 degrees C; the higher germination percentages were verified in the constant temperatures of 30 degrees C and 35 degrees C that didn't differ statistically from the temperature of 25-35 degrees C. The germination was faster at a constant temperature of 30 degrees C.

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Although drought and defoliation stress have been shown to reduce soybean [Glycine max (L.). Merr.] yield, little information has been published regarding their effects on soybean seed quality. Field experiments were conducted in 1986, 1987, and 1989 to evaluate the effect of drought and defoliation (1989 only) stress during soybean seed development on seed germination and vigor. Essex (MG [maturity group] V) and Union (MG III) were grown in 1986 and 1987, and Harper (MG III) and McCall (MG 00) in 1989. Moisture treatments were either well watered or drought stressed during seed development (R5 to R7). In 1989, a total defoliation treatment was also imposed at R6 as an additional stress factor. There were significant reductions in yield and yield components following drought stress in all 3 yr and following defoliation in 1989. Leaf conductance and transpiration also decreased in the drought stress treatments. There was no effect of drought stress on seed germination or seed vigor as measured by accelerated aging germination and the cold test across the four cultivars (determinate and indeterminate) and 3 yr. In 1989 slight changes in 3-d germination and conductivity occurred for some drought stress treatments. Most of this response, however, was related to increased occurrence of hard seed, which does not represent an indication of a change in vigor. Seed germination and vigor were significantly reduced for small, flat, shriveled, and underdeveloped seeds that only occurred following defoliation. These seeds represented a small portion of the seed lot that would normally be removed during conditioning. The data suggest that drought stress would have no effect on seed germination or vigor, unless the stress was severe enough to produce shriveled, flat, underdeveloped seeds.

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The effect of light and temperature on germination of Brachiaria decumbens as well as the action of some dormancy breaking chemicals were tested. Two seed batches stored different times were used. The results show that seeds failed to respond to alternating temperature regimes and different light qualities. Seeds were indifferent to white light at 25 degrees C. KNO3, ethanol and H2SO4 failed to break seed dormancy, whereas KCN and H2O2 partially reduced dormancy of two month stored seeds. The results suggest a metabolic character of dormancy in ''new'' (freshly collected) seeds and confirm the occurence of two types of dormancy in B. decumbens seeds.

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Comm indica L. is an herbaceous species with ornamental and medicinal value, having seeds with a hard seed coat. This study aimed to test the influence of constant temperatures ranging from 5 to 45 C, at 5 C intervals, on the germination of scarified seeds. Data obtained were analyzed through the model of enthalpy of activation in order to obtain the optimum temperature range for germination. The species showed seed germinability in a wide temperature range (10-40 degrees C) being the optimal temperature range between 13.84 and 34.41 degrees C, determined by the enthalpy of activation.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The objective of this study was to investigate the effect of the application of N fertilizer and the cutting age on the dry biomass production of elephant grass. The experiment was performed with the variety Paraiso and planted in a Ferralsol in 2008 in the district of Gurupi (State of Tocantins). Four different rates of urea application were tested (0, 50, 100 and 150 kg ha(-1)) and harvests were made at 120, 150 or 180 days after germination (DAG) of the setts. The dry matter and total N accumulation were evaluated. Dry matter production increased with dose of N, the greatest effect being observed at 180 DAG. There was a linear increase in dry matter (R-2 = 0.75**) and N accumulation (R-2 = 0.96**) permitting a productivity of 34 t ha(-1) of dry matter and an accumulation of 471 kg N ha(-1). The N utilization efficiency (biomass production per unit of applied N) increased with plant age. The higher efficiency of N use favored the quality of biomass production for energy production owing to the higher fibre content.

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The objective of this research was to study the accelerated aging test to evaluate the okra vigor seed. Were used four okra seed lots cv. "Santa Cruz". The initial seed lot quality was evaluate by the moisture content, germination, first germination count, speed of germination index and seedling emergence. The experiment was carried out in two phases: the first one the seeds were submitted to the accelerate aging with and without NaCl salt solution aging periods of 48, 72, 96 and 120 hours, at 41 and 45(0) C temperature. The second phase the best procedures as for as accelerated aging and temperatures were repeated. The period of 72h and 41 C with and without NaCl saturated solution was the most adequate for the lot classification in distint vigor levels.

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The caruncle is a structure present in the micropylar region of Euphorbiaceae seeds. This structure has the ecological function of promoting seed dispersal by ants (myrmecochory), but it is debated whether it also has an agronomical importance influencing seed germination. The influence of the caruncle on castor (Ricinus communis) seed germination was evaluated under low soil water content and high soil salinity. Seeds were germinated at soil water storage capacities varying from 22 to 50% and salinities (NaCl) varying from 0 to 10 dS m(-1) The germination (%) increased following the increments in soil moisture. hut the caruncle had no influence on this process at any moisture level. In one genotype. more root dry mass was produced when caruncle was excised. Increasing salinity reduced the percentage and speed of germination of castor seeds, but no influence of caruncle was detected. No evidence of caruncle influencing castor seed germination was found under low soil water content and high salinity.

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The aim of this paper was to evaluate the effects of the combinations of substrata on germination and development of seedlings of lettuce cultivars. The experiment was carried out in a nursery, in Mato Grosso do Sul State University. There wend compared the following combinations of substrata: washed sand + earthworm humus (2: 1); manure bovine + earthworm humus (2: 1); Plantmax(R) + earthworm humus (2: 1); washed sand + Plantmax(R) (2: 1) and manure bovine + Plantmax(R) (2: 1), and three lettuce cultivars: Crespa sem cabeca, Americana Julia and Baba de Verao. The experimental design was entirely at random, in a 5x3 factorial outline, with four replications and 16 seeds perplot. There were considered for evaluation 10 central seedlings per plot. The characteristics evaluated were: germination percentage (%), index of germination speed (IVE) and dry mass of the whole seedling (g). For all the analyzed variables, the cultivars Baba de Verao and Crespa sem cabeca presented the best results. Manure + humus was the best substratum to obtain seedling with larger development (vigor), althout it did not increase IVE and germination percentage.

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With the advance of agriculture, there was consequent rising in environment degradation. Therefore, It is interesting the use of plants which have in their chemical composition substances capable of helping in the control of weeds to minimize the use of pesticides. Hence, the objective of this paper was to evaluate solutions of leucena extract on weeds that are frequent on soybean crop. The treatments were leucena extracts at 0, 20, 40, 60, 80 and 100% at room temperature water and water heated to 80 degrees C. The extracts were obtained by crushing 200g of leucena leaves and mixing them with one liter of distilled water with the help of a blender. Seeds were kept in B.O.D. at a temperature of 25 degrees C, with a photoperiod of 12 hours of light. Evaluations were done oil a daily basis, from the first to the last day of the experiment, varying according to the species. Results show a negative interference of the extracts, both hot and cold, in the root length of Ipomoea grandifolia, in the percentage of germination and in the root length of Arrowleaf sida and Hair beggarticks. There was no negative interference in the analyzed parameters for soybean seeds. Thus, the results indicate allelopatic potential of Leucaena leuucocephala as an alternative for the management of weeds without interfering on the development of the soybean crop.

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The present study had the aim of testing the effect of different fermentation periods on the germination and vigour of pitomba seeds. The experiment was carried out in the greenhouse of the Seed Section of the Agrarian Sciences Center of the Federal University of Paraiba, Areia, PB. The fruits were picked directly from maternal trees located in the same municipal district, peeled manually and fermented for 24, 48, 72, 96 and 120 hours, in addition to seeds with pulp (without fermentation). The experiment was entirely randomized with four replications of 25 seeds per treatment. After each fermentation period, the seeds were washed in tap water and left in the laboratory environment for 24 hours on paper towels. Water content, germination and vigor (germination velocity index, seedling length and dry mass., relative frequency and medium time of germination) were measured. Less water content was shown in the seeds fermented for 96 hours (38.5%), while the largest germination percentages were observed after 76 hours of fermentation (93%). In relation to vigour, the best values occurred with 86 and 105 hours of fermentation. Fermentation is recommended for up to 105 hours as appropriate to removal of the pitomba seed aril.

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In negatively photoblastic seeds of Cucumis anguria L. the scarification of micropyle overcame the inhibitory effect of white light , although far-red radiation inhibited the germination in both intact and scarified seeds. The moistened testa powder transmitted more far-red than red radiation. Thus the testa could act as a filter of radiation and maintain a high far-red/red ratio at the photosensitive site of the embryo, which must be localized near the micropyle. © 1988 Academia.