Enzymatic and structural characterization of new PLA2 isoform isolated from white venom of Crotalus durissus ruruima

This work reports the structural and enzymatic characterization of a new sPLA2 from the white venom of Crotalus durissus ruruima, nominated PLA2A. The homogeneity of the PLA2A fraction and its molecular mass were initially evaluated by SDS-PAGE and confirmed by MALDI-TOF spectrometry, indicating a molecular mass of 14,299.34 Da. Structural investigation, through circular dichroism spectroscopy, revealed that PLA2A has a high content of alpha helix and beta-turn structures, 45.7% and 35.6% respectively. Its amino acid sequence, determined by Edman degradation and de novo amino acid sequencing, exhibited high identity to PLA2 Cdt F15 from Crotalus durissus terrificus. The enzymatic investigation, conducted using the synthetic substrate 4-nitre-3-(octanoyloxy)benzoic acid, determined its V(max) (7.56 nmoles/min) and K(M) (2.76 mM).Moreover, PLA2A showed an allosteric behavior and its enzymatic activity was dependent on Ca(2+). Intrinsic fluorescence measurements suggested that Ca(2+) induced a significant increase of PLA2A fluorescence, whereas its replacement for Mg(2+), Mn(2+), Sn(2+) and Cd(2+) apparently induced no structural modifications. The optimal pH and temperature for the enzymatic activity of PLA2A were 8.4 and 40 degrees C, respectively, and the minimal concentration of p-BPB and crotapotin that significantly inhibited such activity was 0.75 mM and 0.4 mu M, respectively. In addition, PLA2A showed a significant antibacterial effect that was not strictly dependent on the enzymatic activity of such sPLA2. (c) 2008 Elsevier Ltd. All rights reserved.

Challenges and Opportunities in Transforming Laser System Industry to Deliver Integrated Product and Service Offers

Part 4: Transition Towards Product-Service Systems

Del videojuego a la realidad : sistema interactivo para la seguridad vial

El vertiginoso crecimiento de los centros urbanos, las tecnologías emergentes y la demanda de nuevos servicios por parte de la población plantea encaminar esfuerzos hacia el desarrollo de las ciudades inteligentes. Éste concepto ha tomado fuerza entre los sectores político, económico, social, académico, ambiental y civil; de forma paralela, se han generado iniciativas que conducen hacia la integración de la infraestructura, la tecnología y los servicios para los ciudadanos. En éste contexto, una de las problemáticas con mayor impacto en la sociedad es la seguridad vial. Es necesario contar con mecanismos que disminuyan la accidentalidad, mejoren la atención a incidentes, optimicen la movilidad urbana y planeación municipal, ayuden a reducir el consumo de combustible y la emisión de gases de efecto de invernadero, así como ofrecer información dinámica y efectiva a los viajeros. En este artículo se describen dos (2) enfoques que contribuyen de manera eficiente dicho problema: los videojuegos como juegos serios y los sistemas de transporte inteligente. Ambos enfoques están encaminados a evitar colisiones y su diseño e implementación requieren componentes altamente tecnológicos (e.g. sistemas telemáticos e informáticos, inteligencia artificial, procesamiento de imágenes y modelado 3D).

Smart and sustainable public transport systems through improving level and quality of service

Buses are considered a slow, low comfort and low reliability transport system, thus its negative and por image. In the framework of the 3iBS project (2012), several examples of innovative and/or effective solutions regarding the Level of Service (LoS) were analysed aiming to provide operators, practitioners and policy makers with a set of Good Practice Guidelines to strengthen the competitiveness of the bus in the urban environment. The identification of the key indicators regarding vehicles, infrastructure and operation was possible through the analysis of a set of case studies -among which Barcelona (Spain), Cagliari (Italy), London (United Kingdom), Paris and Nantes (France). A cross comparison between the case studies was carried out for contrasting the level of achievement of the different criteria considered. The information provided on Regulatory, Financial and Technical issues allows the identification of a number of specific factors influencing the implementation of a high quality transport scheme, and set the basis for the elaboration of a set of Guidelines for the implementation of an intelligent, innovative and integrated bus system, including the main barriers to be tackled.

Vacant parking area estimation through background subtraction and transience map analysis

A method for estimating the dimensions of non-delimited free parking areas by using a static surveillance camera is proposed. The proposed method is specially designed to tackle the main challenges of urban scenarios (multiple moving objects, outdoor illumination conditions and occlusions between vehicles) with no training. The core of this work is the temporal analysis of the video frames to detect the occupancy variation of the parking areas. Two techniques are combined: background subtraction using a mixture of Gaussians to detect and track vehicles and the creation of a transience map to detect the parking and leaving of vehicles. The authors demonstrate that the proposed method yields satisfactory estimates on three real scenarios while being a low computational cost solution that can be applied in any kind of parking area covered by a single camera.

Parasitoses internas e frequência de desparasitação em cães do concelho de Vila Franca de Xira, Portugal

Dissertação de Mestrado Integrado em Medicina Veterinária

Aproximación al desarrollo de un sistema de transporte masivo a través de la dinámica de sistemas

El porvenir social y económico de una ciudad depende, en gran medida, de la eficiencia de su sistema de transporte; esto se ve reflejado en la capacidad de transportar personas y bienes de una forma sostenible, con los recursos disponibles. En la actualidad se encuentran en desarrollo sistemas de transporte masivo tipo Bus Rapid Transit [BRT] en siete ciudades colombianas, situación que genera la necesidad de dar seguimiento a su progreso y al crecimiento de su participación en la demanda de viajes unipersonales. El siguiente trabajo busca, a través de una simulación en dinámica de sistemas, describir el desarrollo de un sistema de transporte masivo, con el fin de otorgar una visión acerca del impacto de los parámetros operativos y la reinversión en el sistema y en el desarrollo e incremento de su demanda. Se plantean tres escenarios para evaluar diferentes políticas operativas y de reinversión en el sistema, analizando el comportamiento en su desarrollo.

Simultaneous optimization of colloidal stability and interfacial charge transfer efficiency in photocatalytic Pt/CdS nanocrystals

Colloidal stability and efficient interfacial charge transfer in semiconductor nanocrystals are of great importance for photocatalytic applications in aqueous solution since they provide long-term functionality and high photocatalytic activity, respectively. However, colloidal stability and interfacial charge transfer efficiency are difficult to optimize simultaneously since the ligand layer often acts as both a shell stabilizing the nanocrystals in colloidal suspension and a barrier reducing the efficiency of interfacial charge transfer. Here, we show that, for cysteine-coated, Pt-decorated CdS nanocrystals and Na2SO3 as hole scavenger, triethanolamine (TEOA) replaces the original cysteine ligands in situ and prolongs the highly efficient and steady H2 evolution period by more than a factor of 10. It is shown that Na2SO3 is consumed during H2 generation while TEOA makes no significant contribution to the H2 generation. An apparent quantum yield of 31.5%, a turnover frequency of 0.11 H2/Pt/s, and an interfacial charge transfer rate faster than 0.3 ps were achieved in the TEOA stabilized system. The short length, branched structure and weak binding of TEOA to CdS as well as sufficient free TEOA in the solution are the keys to enhancing colloidal stability and maintaining efficient interfacial charge transfer at the same time. Additionally, TEOA is commercially available and cheap, and we anticipate that this approach can be widely applied in many photocatalytic applications involving colloidal nanocrystals.

Perceived Accessibility of Public Transport as a Potential Indicator of Social Inclusion

Perceived accessibility has been acknowledged as an important aspect of transport policy since the 70s. Nevertheless, very few empirical studies have been conducted in this field. When aiming to improve social inclusion, by making sus-tainable transport modes accessible to all, it is important to understand the factors driving perceived accessibility. Un-like conventional accessibility measures, perceived accessibility focuses on the perceived possibilities and ease of en-gaging in preferred activities using different transport modes. We define perceived accessibility in terms of how easy it is to live a satisfactory life with the help of the transport system, which is not necessarily the same thing as the objec-tive standard of the system. According to previous research, perceived accessibility varies with the subjectively-rated quality of the mode of transport. Thus, improvements in quality (e.g. trip planning, comfort, or safety) increase the per-ceived accessibility and make life easier to live using the chosen mode of transport. This study (n=750) focuses on the perceived accessibility of public transport, captured using the Perceived Accessibility Scale PAC (Lättman, Olsson, & Fri-man, 2015). More specifically, this study aims to determine how level of quality affects the perceived accessibility in public transport. A Conditional Process Model shows that, in addition to quality, feeling safe and frequency of travel are important predictors of perceived accessibility. Furthermore, elderly and those in their thirties report a lower level of perceived accessibility to their day-to-day activities using public transport. The basic premise of this study is that sub-jective experiences may be as important as objective indicators when planning and designing for socially inclusive transport systems.

'Reserve effect' within a temperate marine protected area in the north-eastern Atlantic (Arrabida Marine Park, Portugal)

Benefits of marine protected areas depend on local ecological and socio-cultural aspects which are critical to the success of the protection measures. In particular, before-after comparisons are indispensable to disentangle the effects of protection from those of different physical and ecological characteristics among areas. Using underwater visual surveys, we assessed whether biomass and abundance of temperate reef fish assemblages and target invertebrates increased inside a no-take area in the Arrabida Marine Park (Portugal) 3 to 4 yr after its establishment. Data were compared to a previous study, conducted 10 yr before protection was effective. Control-effect comparisons after reserve establishment showed a positive response of legal-size demersal fish and below legal-size target invertebrates. The first evidence of protection was found in biomass but not in numbers. Non-target groups and below legal-size demersal fish had a significant interaction among reserve and habitat complexity indices for either density or biomass, suggesting a lack of a reserve effect. Before-after comparisons revealed non-significant patterns of increase in numbers of target species compared to non-target ones. The most important commercial species showed the largest increase in density after protection was established. Significantly higher abundances and proportionally heavier individuals of these species were also found inside the reserve in the control-effect comparisons. These findings are reinforced by an increasing trend in landings which are consistent with the early detection of a reserve effect.

Evaluación técnica y económica para analizar la viabilidad del montacargas eléctrico en el sector productivo

Esta tesis pretende demostrar y describir las diferentes características de los montacargas que existen en el mercado, teniendo en cuenta sus ventajas y desventajas, logrando como objetivo analizar la viabilidad de la implementación de los montacargas eléctricos en la industria colombiana a partir de la información brindada sobre las características esenciales de los montacargas eléctricos que hay en el mercado. Así mismo, se quiere mostrar que los montacargas eléctricos generan un ahorro sustancial en comparación con los montacargas convencionales que hay hoy en día en el mercado, logrando así que la industria colombiana reduzca sus gastos en un porcentaje mediante la implementación de las nuevas referencias de montacargas eléctricos los cuales serian una alternativa para las compañías colombianas. Mediante una detallada revisión conceptual, se mostrara la viabilidad de los montacargas eléctricos frente a los otros tipos de montacargas, teniendo como premisa el conocimiento de lo que hoy en día requieren las industrias colombianas, y cumpliendo así con una detallada comparación en donde se enfatice la diferenciación de los montacargas eléctricos con otros tipos que existen y se logre brindar una clara percepción de estos junto con las ventajas de estos vehículos para lograr así brindar mayor información a la industria colombiana sobre las ventajas de la implementación de los montacargas eléctricos en la industria colombiana.

Planning Processes for Transport Solutions An Application To the City of Bologna

Mathematical models and the involved methods applied to real contexts are essential tools for designing and evaluating solutions concerning physical elements and/or organizational components of transportation systems. To deal with this, the systems engineering approach is used, which considers the relationships among the transportation system elements and their performances. This approach allows quantifying the effects of transportation projects by taking into account the intrinsic complexity of the transportation system and then assessing the effects of solutions to solve – or mitigate – transportation problems. This thesis focuses on the application of the transport system engineering approach to a real city – Bologna, in northern Italy – in order to: 1. simulate the current transportation system conditions (status quo); 2. compare and assess the results obtained by two different approaches for simulating the link traffic flows on the road transportation network and their related impacts (externalities) 3. identify potential solutions to solve critical aspects, particularly in terms of traffic flow congestion and related environmental impacts (findings)

Sicurezza di rete, analisi del traffico e monitoraggio.

Il lavoro è stato suddiviso in tre macro-aree. Una prima riguardante un'analisi teorica di come funzionano le intrusioni, di quali software vengono utilizzati per compierle, e di come proteggersi (usando i dispositivi che in termine generico si possono riconoscere come i firewall). Una seconda macro-area che analizza un'intrusione avvenuta dall'esterno verso dei server sensibili di una rete LAN. Questa analisi viene condotta sui file catturati dalle due interfacce di rete configurate in modalità promiscua su una sonda presente nella LAN. Le interfacce sono due per potersi interfacciare a due segmenti di LAN aventi due maschere di sotto-rete differenti. L'attacco viene analizzato mediante vari software. Si può infatti definire una terza parte del lavoro, la parte dove vengono analizzati i file catturati dalle due interfacce con i software che prima si occupano di analizzare i dati di contenuto completo, come Wireshark, poi dei software che si occupano di analizzare i dati di sessione che sono stati trattati con Argus, e infine i dati di tipo statistico che sono stati trattati con Ntop. Il penultimo capitolo, quello prima delle conclusioni, invece tratta l'installazione di Nagios, e la sua configurazione per il monitoraggio attraverso plugin dello spazio di disco rimanente su una macchina agent remota, e sui servizi MySql e DNS. Ovviamente Nagios può essere configurato per monitorare ogni tipo di servizio offerto sulla rete.

Studio preliminare di un sistema di monitoraggio e controllo del traffico di superficie nell’area di manovra dell’aeroporto di Fiumicino

Questo lavoro ha analizzato il modulo di localizzazione ed identificazione dei mezzi circolanti in area di manovra e la tecnologia da impiegare per ottenere lo scambio dei dati tra le unità imbarcate ed il server centrale. L’attività è stata svolta in collaborazione con ADR-Aeroporti di Roma, gestore aeroportuale di Fiumicino. L’obiettivo è quello di ottenere, in tempo reale, la conoscenza della posizione e della traiettoria di tutti i mezzi circolanti in area di manovra per creare un sistema automatizzato di monitoraggio, in grado di controllare sia eventuali invasioni delle piste e dei raccordi principali sia rischi di potenziali collisioni tra velivoli e mezzi, garantendo la minima separazione richiesta dalle normative e fornendo agli operatori l’indicazione del rischio rilevato. La prima parte della tesi espone lo scenario europeo per quanto riguarda le tecnologie utilizzate per la sorveglianza di velivoli in area di manovra, la seconda descrive la tecnologia più diffusa e promettente a livello internazionale, proposta per l’aeroporto di Fiumicino, mentre nella terza parte tale tecnologia viene analizzata e sviluppata appunto per lo scenario preso in considerazione.