Low frequency of E-cadherin alterations in familial breast cancer

In order to explore the possible role of E-cadherin in familial cancer, 19 familial breast cancer patients, whose tumours demonstrated loss of heterozygosity (LOH) at the E-cadherin locus, were screened for germline mutations. No pathogenic germline alterations were detected in these individuals. However, a somatic mutation was found (49-2A→C) in one of the tumours. This tumour showed a pattern of both ductal and lobular histology. Another 10 families with cases of breast, gastric and colon cancer were also screened for germline mutations, and no mutations were found. A missense mutation in exon 12 of E-cadherin (1774G→A; Ala592Thr) was previously found in one family with diffuse gastric cancer, and colon and breast cancer. An allelic association study was performed to determine whether the Ala592Thr alteration predisposes to breast cancer. In total, we studied 484 familial breast cancer patients, 614 sporadic breast cancer patients and 497 control individuals. The frequencies of this alteration were similar in these groups. However, a correlation between the Ala592Thr alteration and ductal comedo-type tumour was seen. These results, together with previously reported studies, indicate that germline mutations and, more commonly, somatic mutations in E-cadherin may have an influence on the behaviour of the tumours, rather than predispose to breast cancer.

A mouse model for adenovirus gene delivery

The cellular attachment receptor for adenovirus (Ad), Coxsackie adenovirus receptor (CAR), required for delivery of Ad into primary cells, is not present on all cell types, thus restricting Ad-gene delivery systems. To circumvent this constrain, a transgenic mouse has been generated that expresses a truncated human CAR in all tissues analyzed. These mice allowed efficient in vitro infections at low multiplicities into lymphoid, myeloid, and endothelial cells. Furthermore, in vivo administration of Ad-vectors results in infection of macrophages, lymphocytes, and endothelial cells. In addition, tail vein injection resulted in targeting of virus into previously inaccessible areas, such as the lung and the capillaries of the brain. The CAR transgenic mice will be useful for rapid functional genomic analysis in vivo, for testing the efficacy of gene therapy procedures or as a source of easily transducible cells.

Evidence that two present-day components needed for the genetic code appeared after nucleated cells separated from eubacteria.

The trinucleotide/amino acid relationships of the present-day genetic code are established by the amino-acylation reactions of tRNA synthetases, whereby each of 20 specific amino acids is attached to its cognate tRNAs, which bear anticodon trinucleotides. Because of its universality, the appearance of the modern genetic code is thought to predate the separation of prokaryotic and eukaryotic organisms in the universal phylogenetic tree. In the light of new sequence information, we present here a phylogenetic analysis that shows an unusual picture for tyrosyl- and tryptophanyl-tRNA synthetases. Ij particular, the eukaryotic tyrosyl- and tryptophanyl-tRNA synthetases are more related to each other than to their respective prokaryotic counterparts. In contrast, each of the other 18 eukaryotic synthetases is more related to its prokaryotic counterpart than to any eukaryotic synthetase specific for a different amino acid. Our results raise the possibility that present day tyrosyl- and tryptophanyl-tRNA synthetases appeared after the separation of nucleated cells from eubacteria. The results have implications for the development of the genetic code.

Orchestration of gene expression across the seasons : Hypothalamic gene expression in natural photoperiod throughout the year in the Siberian hamster

Funding for work in the laboratory of PB was supported by Scottish Government Rural and Environment Science and Analytical Services Division, BBSRC (grant BB/M001504/1), British Society for Neuroendocrinology (research visit grant to IP). Work in the laboratory of SS was supported by a grant from the DFG (Ste 331/8-1). We thank Siegried Hilken, Marianne Brüning, Dr. Esther Lipokatic-Takacs and Dr. Frank Scherbarth at UVMH for technical assistance. We thank Graham Horgan of Bioinformatics, Statistics Scotland for assistance with some of statistical tests.

[ Majmūʻat rasāʼil]

li-Maḥmūd Afandī al-Ḥamzāwī.

Jām-i Jam-i Hindūstān

az ʻAlī Khān Ḥijāzī mulaqqab bih Viqār al-Mulk.

Kaside icazeti : manuscript, 1882 or 3

An ijāzah issued by Aḥmad Ḍiyāʼ al-Dīn ibn al-Ḥājj ʻAbd al-Raḥmān-zādah Muṣṭafá to ʻAbd al-Raḥmān ibn Maḥmūd al-Rifqī (?) for Qaṣīdat al-Burdah of al-Būṣīrī. Includes a Turkish section which explains how the Qaṣīdah should be read and for what purposes.

Kitāb Laṭāʼif akhbār al-uwal fī-man taṣarrafa fī Miṣr min al-duwal / taʼlīf sayyidinā wa-mawlānā al-ʻālim al-ʻallāmah Muḥammad ibn ʻAbd al-Muʻṭī ibn Abī al-Fatḥ ibn Aḥmad ibn ʻAbd al-Ghanī ibn ʻAlī al-Isḥāqī al-Shāfiʻī : manuscript, 1733

بسم الله الرحمن الرحيم فهو حبي اكفي الحمد لله الملك العزيز في ملكه واقتذاره الذي ملك الوجود يقوته واوجد بارادته واختياره... :Incipit

Netâicü'l-fünûn / li-Nev'î Efendi : manuscript, undated

Concise encyclopedia on twelve sciences: 1. history; 2. philosophy; 3. astronomy; 4. theology; 5. principles of law; 6. controverted points; 7. exegesis; 8. mysticism; 9. interpretation of dreams; 10. magic, charm, and medecine; 11. agriculture; 12. astrology and divination.

Irtiqāʼ al-siyādah fī ʻilm uṣūl al-naḥw : manuscript, [ca. 1675]

Written in one column, 21 lines per page, in black and red.

Hādhā kitāb ʻIqd al-jawhar al-thamīn fī arbaʻīn ḥadīthan min aḥādīth Sayyid al-mursalīn jamʻ al-Shaykh al-ʻAllāmah al-Imām muḥaddith Bilād al-Shām Ismāʻīl al-Jarrāḥī al-shahīr bi-al-ʻAjlūnī : manuscript, 1849

Written in one column, 19 lines per page, in black rubricated in red.

Thabat al-ʻAṭṭār : manuscript, 1849

1 unbound unsewn quire.

Thabat al-Shaykh Muḥammad al-Bābilī : manuscript, undated

Written in one column, 49 lines per pages, in black.

Risālah : manuscript, [ca. 1918]

Title supplied by cataloger.