Relatório de estágio em educação física realizado na Escola Secundária Jaime Moniz

Este documento reflete o ano de estágio pedagógico que realizámos na Escola Secundária Jaime Moniz no ano letivo 2012/2013. Através do relatório apresentamos as nossas principais dificuldades, estratégias aplicadas e reflexões sobre todas as atividades que o estágio envolveu e como todo o processo contribuiu para o nosso desenvolvimento profissional e pessoal. Após a nossa perspetiva fundamentada sobre o que representa o estágio pedagógico, caraterizamos a escola que nos acolheu e estruturamos as atividades que realizámos ao longo do ano letivo. Estas encontram-se organizadas por diferentes capítulos, correspondentes às linhas programáticas do estágio, nos quais procuramos refletir sobre os aspetos do planeamento, da operacionalização e da gestão de cada atividade. O relatório inclui igualmente as nossas considerações finais e recomendações, que surgem após a reflexão sobre todas as experiências vividas no ano letivo. Finalizamos o documento com as respetivas referências bibliográficas que serviram todo o seu constructo teórico.

Relatório de estágio de educação física realizado na Escola Secundária Jaime Moniz

O presente relatório científico de estágio em educação física, que se realizou na Escola Secundária Jaime Moniz no ano letivo 2012/2013, visa refletir sobre todo o processo formativo de estágio, tendo por base uma fundamentação científica. Com este relatório pretendemos expor as experiencias vividas, as competências adquiridas, a gestão de todo o processo de ensino-aprendizagem, estratégias utilizadas e a reflexão, num processo de transformação tanto para os professores, como para os alunos. Ao longo do estágio, foram por nós desenvolvidas atividades em diferentes âmbitos, com diferentes intencionalidades, sendo estas curriculares, extracurriculares e também de cariz cientifico-pedagógico. Numa fase inicial, serão introduzidos assuntos como o enquadramento e a importância do estágio pedagógico, os objetivos e propósitos, seguida da caraterização da escola de acolhimento e as atividades desenvolvidas ao longo do ano letivo. Posteriormente iremos apresentar, analisar os processos utilizados em cada uma das atividades desenvolvidas no estágio, refletindo as vantagens e desvantagens dos mesmos, bem como as principais ilações e ensinamentos retirados a nível profissional e pessoal. Por fim, para concluir o presente relatório, serão expostas algumas considerações finais e sugestões futuras relativamente ao estágio, seguido das referências bibliográficas que fizeram parte do suporte científico deste trabalho.

Relatório de estágio de biologia e geologia realizado na Escola Secundária Jaime Moniz

O estágio pedagógico é uma etapa fundamental na formação inicial pois promove a transição do aluno a professor, permitindo a primeira experiência com a profissão docente e a aplicação prática num contexto real de ensino dos conhecimentos adquiridos durante o curso. O presente relatório visa descrever de forma contextualizada, as atividades de ensino e aprendizagem desenvolvidas durante o estágio pedagógico realizado no ano letivo 2012/2013 na Escola Secundária Jaime Moniz (ESJM), no âmbito do Mestrado em Ensino de Biologia e Geologia no 3º Ciclo do Ensino Básico e no Ensino Secundário. Pretende também refletir criticamente sobre as experiências de ensino-aprendizagem realizadas e sobre as vivências, dificuldades sentidas, competências adquiridas e aspetos a melhorar. Este relatório descreve os intervenientes neste processo e as atividades de ensino aprendizagem desenvolvidas. Uma das atividades de maior importância durante o estágio pedagógico foi a Prática Letiva (PL), pois permitiu a aquisição de várias competências necessárias à intervenção pedagógica, através do Processo de Ensino-Aprendizagem e da Assistência às Aulas. Por sua vez, as Atividades de Integração no Meio (AIM), compostas pela Caracterização da Turma e pelo Estudo de Caso foram particularmente importantes para conhecer os alunos da turma, e adequar a intervenção pedagógica às suas características. As Atividades de Intervenção na Comunidade Escolar (AICE) proporcionaram a oportunidade de desenvolver competências na área da organização de eventos e promoveram a integração na Escola. A Atividade de Natureza Científico-Pedagógica (ANCP) foi importante sobretudo no desenvolvimento de competências investigativas. O estágio pedagógico constitui para o professor em formação um importante processo de aprendizagem, pois possibilita a tomada de consciência das funções que este deve desempenhar na escola, e a aquisição e desenvolvimento de competências pessoais e profissionais necessárias para o desempenho da profissão.

Gold nanoparticles decorated with oligo(ethylene glycol) thiols:enhanced Hofmeister effects in colloid−protein mixtures

Oligo(ethylene glycol) (OEG) thiol self-assembled monolayer (SAM) decorated gold nanoparticles (AuNPs) have potential applications in bionanotechnology due to their unique property of preventing the nonspecific absorption of protein on the colloidal surface. For colloid-protein mixtures, a previous study (Zhang et al. J. Phys. Chem. A 2007, 111, 12229) has shown that the OEG SAM-coated AuNPs become unstable upon addition of proteins (BSA) above a critical concentration, c*. This has been explained as a depletion effect in the two-component system. Adding salt (NaCl) can reduce the value of c*; that is, reduce the stability of the mixture. In the present work, we study the influence of the nature of the added salt on the stability of this two-component colloid-protein system. It is shown that the addition of various salts does not change the stability of either protein or colloid in solution in the experimental conditions of this work, except that sodium sulfate can destabilize the colloidal solutions. In the binary mixtures, however, the stability of colloid-protein mixtures shows significant dependence on the nature of the salt: chaotropic salts (NaSCN, NaClO4, NaNO3, MgCl2) stabilize the system with increasing salt concentration, while kosmotropic salts (NaCl, Na2SO4, NH4Cl) lead to the aggregation of colloids with increasing salt concentration. These observations indicate that the Hofmeister effect can be enhanced in two-component systems; that is, the modification of the colloidal interface by ions changes significantly the effective depletive interaction via proteins. Real time SAXS measurements confirm in all cases that the aggregates are in an amorphous state.

The influence of composition on the optical properties of electrodeposited gold

In industry the colour of a gold alloy electrodeposit is checked by visual comparison with standard panels. The aims of the present work have been to access the application of spectrophotmetric techniques to the measurement of the colour of gold alloy electrodeposits and to examine the factors that influence the colour of thin deposits. The minimum thickness of deposit required to produce its final colour and completely hide the underlying substrate was measured and found to depend on the nature of the substrate, the plating solution and the operating conditions. Bright and matt electrodeposits were studied. The influence of alloying gold by adding copper, silver and indium to the plating solution were investigated. CIE chromaticity coordinates were calculated from spectrophotometric data using a computer programme written for the purpose. The addition of silver to a simple gold bath caused the colour of the deposit to change from yellow through green to near white in a smooth progression as the amount of silver in solid solution steadily increased. The colour of deposits formed when additions of copper were made was complicated by the formation of intermediate phases. À colour in the blue region of the spectrum was obtained in a few experiments investigating the influence of indium additions to the gold bath.

Gold mineralisation in the Caledonides of the British Isles with special reference to the Dolgellau Gold-belt, North Wales and the Southern Uplands, Scotland. Available in 2 volumes.

Two aspects of gold mineralisation in the Caledonides of the British Isles have been investigated: gold-telluride mineralisation at Clogau Mine, North Wales; and placer gold mineralisation in the Southern Uplands, Scotland. The primary ore assemblage at Clogau Mine is pyrite, arsenopyrite, cobaltite, pyrrhotine, chalcopyrite, galena, tellurbismuth, tetradymite, altaite, hessite, native gold, wehrlite, hedleyite, native bismuth, bismuthunite and various sulphosalts. The generalised paragenesis is early Fe, Co, Cu, As and S species, and later minerals of Pb, Bi, Ag, Au, Te, Sb. Electron probe micro-analysis (EPMA) of complex telluride-sulphide intergrowths suggests that these intergrowths formed by co-crystallisation/replacement processes and not exsolution. Minor element chemical variation, in the sulphides and tellurides, indicates that antimony and cadmium are preferentially partitioned into telluride minerals. Mineral stability diagrams suggest that during gold deposition log bf aTe2 was between -7.9 and -9.7 and log bf aS2 between -12.4 and -13.8. Co-existing mineral assemblages indicate that the final stages of telluride mineralisation were between c. 250 - 275oC. It is suggested that the high-grade telluride ore shoot was the result of remobilisation of Au, Bi, Ag and Te from low grade mineralisation elsewhere within the vein system, and that gold deposition was brought about by destabilisation of gold chloride complexes by interaction with graphite, sulphides and tellurbismuth. Scanning electron microscopy of planer gold grains from the Southern Uplands, Scotland, indicates that detailed studies on the morphology of placer gold can be used to elucidate the history of gold in the placer environment. In total 18 different morphological characteristics were identified. These were divided on an empirical basis, using the relative degree of mechanical attrition, into proximal and distal characteristics. One morphological characteristic (a porous/spongy surface at high magnification) is considered to be chemical in origin and represent the growth of `new' gold in the placer environment. The geographical distribution of morphological characteristics has been examined and suggests that proximal placer gold is spatially associated with the Loch Doon, Cairsphairn and Fleet granitoids. Quantitative EPMA of the placer gold reveals two compositional populations of placer gold. Examination of the geographical distribution of fineness suggests a loose spatial association between granitoids and low fineness placer gold. Also identified was chemically heterogeneous placer gold. EPMA studies of these heterogeneities allowed estimation of annealing history limits, which suggest that the heterogeneities formed between 150 and 235oC. It is concluded, on the basis of relationships between morphology and composition, that there are two types of placer gold in the Southern Uplands: (i) placer gold which is directly inherited from a hypogene source probably spatially associated with granitoids; and (ii) placer gold that has formed during supergene processes.

Analysis of radicals and radical reaction products in cell signalling and biomolecular damage:the long hard road to gold-standard measures

The field of free radical biology and medicine continues to move at a tremendous pace, with a constant flow of ground-breaking discoveries. The following collection of papers in this issue of Biochemical Society Transactions highlights several key areas of topical interest, including the crucial role of validated measurements of radicals and reactive oxygen species in underpinning nearly all research in the field, the important advances being made as a result of the overlap of free radical research with the reinvigorated field of lipidomics (driven in part by innovations in MS-based analysis), the acceleration of new insights into the role of oxidative protein modifications (particularly to cysteine residues) in modulating cell signalling, and the effects of free radicals on the functions of mitochondria, extracellular matrix and the immune system. In the present article, we provide a brief overview of these research areas, but, throughout this discussion, it must be remembered that it is the availability of reliable analytical methodologies that will be a key factor in facilitating continuing developments in this exciting research area.

Gold nanoparticles decorated with oligo(ethylene glycol) thiols:kinetics of colloid aggregation driven by depletion forces

We have studied the kinetics of the phase-separation process of mixtures of colloid and protein in solutions by real-time UV-vis spectroscopy. Complementary small-angle X-ray scattering (SAXS) was employed to determine the structures involved. The colloids used are gold nanoparticles functionalized with protein resistant oligo(ethylene glycol) (OEG) thiol, HS(CH(2))(11)(OCH(2)CH(2))(6)OMe (EG6OMe). After mixing with protein solution above a critical concentration, c*, SAXS measurements show that a scattering maximum appears after a short induction time at q = 0.0322 angstrom(-1) stop, which increases its intensity with time but the peak position does not change with time, protein concentration and salt addition. The peak corresponds to the distance of the nearest neighbor in the aggregates. The upturn of scattering intensities in the low q-range developed with time indicating the formation of aggregates. No Bragg peaks corresponding to the formation of colloidal crystallites could be observed before the clusters dropped out from the solution. The growth kinetics of aggregates is followed in detail by real-time UV-vis spectroscopy, using the flocculation parameter defined as the integral of the absorption in the range of 600-800 nm wavelengths. At low salt addition (<0.5 M), a kinetic crossover from reaction-limited cluster aggregation (RLCA) to diffusion-limited cluster aggregation (DLCA) growth model is observed, and interpreted as being due to the effective repulsive interaction barrier between colloids within the depletion potential. Above 0.5 M NaCl, the surface charge of proteins is screened significantly, and the repulsive potential barrier disappeared, thus the growth kinetics can be described by a DLCA model only.