State of logistics and transport in the Baltic Sea region in 2025

The world’s pace of change is accelerating and new innovations, inventions and technologies come about every day. Change is unavoidable. It is difficult to keep up and even more difficult to prepare for the future. Even though it is not possible to know exactly what will happen in the future, by studying futures people can better anticipate what might lie ahead. By making decisions and realizing the consequences of their choices today, people and governments are able to actively decide how they will act in the future. Both opportunities and pitfalls lie ahead, which encourages actors to make more farsighted decisions. The Baltic Sea region is an interesting area for futures studies. It comprises 11 nations and more than 100 million inhabitants and entails countries with advanced, high-income economies, like Finland, Germany and Denmark, and developing economies, like Russia, Latvia and Lithuania. The western, eastern, northern and southern parts of the region are separated by the Baltic Sea, which at the same time represents a barrier and a facility for trade and travel between the countries belonging to the region The purpose of this study was to uncover the most probable future of transport and logistics in the Baltic Sea region in 2025 by using the Delphi method. Altogether 109 responses were collected in two separate instances from experts in all the Baltic Sea region countries, 56 of whom were defined as academic respondents and 53 of whom business respondents. Only minor differences in the opinions of academic and business experts were discovered, and the larger differences lie between eastern and western response groups. The Baltic Sea region is a very heterogeneous region and the division is clearest between East and West, which differ in political, economic, social, technological and environmental aspects. The probable future of the Baltic Sea region presented in this study is coherent with previous studies on the same subject. The future of the Baltic Sea region in terms of logistics and transport looks quite bright according to the experts who participated in the study. Trade volumes will grow and the importance of logistics and transport to the competitiveness of the region will increase. Respondents from eastern countries seemed to be more optimistic about the future in general. Most differences between opinions could be explained by the gap in technological and infrastructural development between the East and West. As eastern countries are less-developed in some parts of their economies, it is easier for them to improve the technical condition of infrastructure by merely catching up with the western countries.

Weed Phytosociological and Floristic Survey in Agricultural Areas of Southwestern Goiás Region

The use of the Roundup Ready(r) technology and the cultivation of a second crop influence the floristic composition of weed communities in Brazilian Central-West region cropping systems. This study has aimed to diagnose the dominant weed species in southwestern Goiás in areas of genetically-modified and conventional soybeans, using phytosociological and floristic surveys. Weed sampling was obtained by collecting all the plants present within a 0.5 m hollow frame, randomly thrown 20 times in each of thirty-five agricultural areas in the 2012/2013 harvest. Field survey was carried out in three periods: before desiccation for soybean sowing, before postemergence herbicide in soybean first application and before postemergence herbicide application in late harvest. A total of 525 m2 was inventoried and 3,219 weeds were collected, which included 79 species, 58 genera and 28 families. Families Poaceae, Asteraceae, Euphorbiaceae, Fabaceae, Amaranthaceae, were the most representative in the survey. Species Cenchrus echinatus, Glycine max, Chamaesyce hirta, Commelina benghalensis, and Alternanthera tenella stood out in importance. The RR+millet soybean treatment had the highest number of species (44), while the conventional soybean + sorghum treatment had the lowest number of species (18). The highest number of species was recorded in first sampling period. Treatments conventional soybean + maize and conventional soybean + millet showed higher similarity (70%), while treatments RR soybean + millet and conventional soybean + sorghum showed the least (51%). Species of difficult control were recorded in all cultivation systems analyzed.

Leaching of Sulfentrazone in Soils from the Sugarcane Region in the Northeast Region of Brazil

Sulfentrazone leaching potential is dependent on soil properties such as strength and type of clay, organic matter content and pH, and may result in ineffectiveness of the product and contamination of groundwater. The objective of this study was to evaluate sulfentrazone leaching in five soils of the sugarcane region in the Northeast Region of Brazil, with different physical and chemical properties, by means of bioassay and high-performance liquid chromatography (HPLC) resolution. The experiment was conducted in a split plot in a completely randomized design. The plots had PVC columns with a 10 cm diameter and being 50 cm deep, filled with five different soil classes (quartzarenic neosol, haplic cambisol, yellowish-red latosol, yellowish-red acrisol, and haplic gleysol), and subplots for 10 depths in columns, 5 cm intervals. On top of the columns, sulfentrazone application was conducted and 12 hours later there was a simulated rainfall of 60 mm. After 72 hours, the columns were horizontally placed and longitudinally open, divided into sections of 5.0 cm. In the center of each section of the columns, soil samples were collected for chromatographic analyses and sorghum sowing was carried out as an indicator plant. The bioassay method was more sensitive to detect the presence of sulfentrazone in an assessment for chromatography soil, having provided greater herbicide mobility in quartzarenic neosol and yellowish-red latosol, whose presence was detected by the indicator plant to a depth of 45 and 35 cm, respectively. In the other soils, sulfentrazone was detected up to 20 cm deep. The intense mobility of sulfentrazone in quartzarenic neosol may result in herbicide efficiency loss in the soil because the symptoms of intoxication and the amount of herbicide detected via silica were highest between 15 cm and 35 cm depth regarding the soil surface layer (0-10 cm), indicating that sulfentrazone should be avoided in soils with such characteristics.

Fedora 4 as a Shared Linked Data repository for the Art Institute of Chicago Collections

Presentation at Open Repositories 2014, Helsinki, Finland, June 9-13, 2014

Spatial genetic structure within natural populations of Machaerium villosum Vog. (Leguminosae) at Moji-Guaçu region, SP, Brazil

A spatial autocorrelation study of enzyme loci detected by starch gel electrophoresis was performed to verify the occurrence of spatial genetic structure within two natural populations of Machaerium villosum Vog. The sampled populations were termed "Antropic Model (MA)" and "Natural Model (MN)" and they are situated in Campininha Farm areas, at Moji-Guaçu municipality, 22°10'43''-22°18'19'' S and 47°8'5"-47°11'34" W, in the state of São Paulo. Ten polymorphic loci in the MA population and nine polymorphic loci in the MN population were assessed by Moran's I autocorrelation statistic. No spatial autocorrelation was detected among individuals within sampled populations. Results are in line with other studies in woody species from tropical rain forest.

Biomass and chemical composition of Nitella furcata subsp. mucronata var. mucronata f. oligospira (A. Braun) R. D. Wood (Chlorophyta, Characeae) in the littoral region of Ninféias Pond, São Paulo, Southeast Brazil

Temporal variation of Nitella furcata (Roxburgh ex Bruzelius) C. Agardh emend. R. D. Wood subsp. mucronata (A. Braun) R. D. Wood var. mucronata f. oligospira (A. Braun) R. D. Wood biomass and chemical composition were studied at the Ninféias Pond (23°38'18.9" S, 46°37'16.3" W), a mesotrophic reservoir located in the Parque Estadual das Fontes do Ipiranga Biological Reserve, Municipality of São Paulo, Southeast Brazil. Plants were collected monthly from October 1996 to October 1997 at three fixed stations of reservoir's littoral region. Charophyte biomass spatial distribution pattern did not vary significantly throughout the study period at all sampling stations. As to seasonal variation, the highest average values of the total alga biomass (98.35-266.06 g m-2 DW) were registered during the rainy season, whereas lowest values (48.86-170.56 g m-2 DW) were in the dry season. P values varied from 23.8 to 225.2 mg m-2 and C from 139 to 353 mg m-2. During the rainy season, greatest air and water temperature, rain precipitation, turbidity and dissolved inorganic nitrogen values were measured, constituting the best conditions for charophyte growth. Water temperature and nutrient availability in the reservoir played a decisive role towards growth and accumulation of algal biomass.

Corticolous green algae from tropical forest remnants in the northwest region of São Paulo State, Brazil

The algae inhabit a wide variety of terrestrial environments and substrates; however the taxonomic knowledge for tropical regions is still scarce. This survey was conducted in ten forest remnants in São Paulo State where visible growths of algae and bryophytes were collected and studied for the main algal components of the communities. Results reveal the occurrence of nine species of green algae, distributed through the class Trebouxiophyceae (one species), Charophyceae (one species) and Ulvophyceae (seven species). Desmococcus olivaceus (Persoon ex Archerson) J. R. Laundon and Printzina effusa (Krempelhüber) Thompson & Wujek are new records for Brazil. The most frequent organisms found in the areas pertain to Trentepohliales that is mainly represented by Trentepohlia species. On the basis of results found, it is recommended that such communities receive more attention in future investigations to improve the knowledge about this important group of primary producers.

The genus Stachybotrys (anamorphic fungi) in the semi-arid region of Brazil

(The genus Stachybotrys (anamorphic fungi) in the semi-arid region of Brazil). Stachybotrys is characterized by macronematous, mononematous, unbranched or branched conidiophores, with discrete terminal and phialidic conidiogenous cells, and aseptate, reniform, ellipsoidal to spherical, smooth or verrucose conidia, which are produced in a slimy mass. Eight species have been reported from Brazil, occurring in the soil, air and leaf litter. During investigation of conidial fungi on decaying leaf litter in semi-arid areas of Brazil nine species were found: S. bisbyi (Sriniv.) G.L. Barron, S. chartarum (Ehrenb.) S. Hughes, S. globosa P. C. Misra & S. K. Srivast., S. kampalensis Hansf., S. longispora Matsush., S. nephrospora Hansf., S. nilagirica Subram., Stachybotrys parvispora S. Hughes and S. verrucispora Matsush. Stachybotrys nilagirica is a new record from Brazil. Descriptions, comments, geographic distribution and illustrations are presented for above mentioned species. A key for all species recorded in semi-arid region of Brazil is presented.

Floristic diversity of two crystalline rocky outcrops in the Brazilian northeast semi-arid region

Floristic composition and structure of vegetation were studied in two rocky outcrop areas in the semi-arid region of northeastern Brazil. From April 2007 to September 2008, 18 monthly field trips were carried out. Vascular plants were randomly collected throughout the outcrop areas. For structural analysis, 30 plots of 1 × 1 m were set in the vegetation islands. The checklist presented combines 211 species (69 families and 168 genera), although only 56 species were collected in the plots. Fabaceae (18 spp.; 8.5%), Asteraceae (17 spp.; 8%), Orchidaceae (13 spp.; 6.1%), Euphorbiaceae (13 spp.; 6.1%), Bromeliaceae (10 spp.; 4.7%), and Poaceae (eight spp.; 3.8%) are the richest families. Overall, 1,792 shrub and herbaceous specimens were counted in the plots. The Shannon-Wiener (H) diversity index values were 2.572 and 2.547 nats individual-1. The species that presented the highest absolute abundance values (number of plants) had low frequencies in the plots and vice-versa. The biological spectrum had a high proportion of phanerophytes and therophytes, followed by cryptophytes, chamaephytes, and hemicryptophytes. The studied flora shares floristic components similar to other rocky outcrop areas of the semi-arid region in northeastern Brazil, including in relation to dominant groups in the vegetation structure.

Pigmented Euglenophytes in a natural and shallow lake in the semiarid region of Paraíba State, Brazil

A floristic survey of the class Euglenophyceae in Panati Lake (7º11'14" S and 36º49'52" W) is presented. The samples were collected between November 2005 and April 2007 along the margins of the lake, and a total of 29 taxa were collected, described, and illustrated, comprising 11 specifics and 18 infraspecifics, with five typical and 13 non-typical varieties. The genus Trachelomonas showed the greatest richness (13 taxa), followed by the genus Lepocinclis (seven taxa). All of the 29 taxa cataloged had been previously reported for Brazil, most with wide geographical distributions, but all were new records for Paraíba State, and 25 new for northeastern Brazil - reflecting the need for more taxonomic studies of the Euglenophyceae in these locations.

Spatial variations at different observational scales and the seasonal distributions of stream macroalgae in a Brazilian subtropical region

We examined the relationships between environmental variations in lotic ecosystems with the seasonal dynamics of macroalgae communities at different spatial scales: drainage basin of two rivers (Rio das Pedras and Rio Marrecas), shading (open and shaded stream segments), mesohabitat (riffles and pools), and microhabitats. Data collections were made on a monthly basis between January and December/2007. A total of 16 taxa were encountered (13 species and 3 vegetative groups). All of the biotic parameters (richness, abundance, diversity, equitability, and dominance) were found to be highly variable at all of the spatial scales evaluated. On the other hand, abiotic variables demonstrated differences only at mesohabitat (in terms of current velocity) and shaded habitat (in terms of irradiance) scales. The seasonality of the macroalgae community structure was strongly influenced by microhabitat variables (current velocity, substrate H', and irradiance), demonstrating their importance over time and at different scales. Regional variables (temperature, oxygen saturation, specific conductance, pH, and turbidity) were found to have little influence on the temporal dynamics of the macroalgae communities evaluated.

A novel polymorphism in the coding region of the vasopressin type 2 receptor gene

Nephrogenic diabetes insipidus (NDI) is a rare disease characterized by renal inability to respond properly to arginine vasopressin due to mutations in the vasopressin type 2 receptor (V2(R)) gene in affected kindreds. In most kindreds thus far reported, the mode of inheritance follows an X chromosome-linked recessive pattern although autosomal-dominant and autosomal-recessive modes of inheritance have also been described. Studies demonstrating mutations in the V2(R) gene in affected kindreds that modify the receptor structure, resulting in a dys- or nonfunctional receptor have been described, but phenotypically indistinguishable NDI patients with a structurally normal V2(R) gene have also been reported. In the present study, we analyzed exon 3 of the V2(R) gene in 20 unrelated individuals by direct sequencing. A C®T alteration in the third position of codon 331 (AGC®AGT), which did not alter the encoded amino acid, was found in nine individuals, including two unrelated patients with NDI. Taken together, these observations emphasize the molecular heterogeneity of a phenotypically homogeneous syndrome

Study of a region on yeast chromosome XIII that complements pet G199 mutants (COX7) and carries a new non-essential gene

The mutants of Saccharomyces cerevisiae assigned to complementation group G199 are deficient in mitochondrial respiration and lack a functional cytochrome oxidase complex. Recombinant plasmids capable of restoring respiration were cloned by transformation of mutants of this group with a yeast genomic library. Sequencing indicated that a 2.1-kb subclone encompasses the very end (last 11 amino acids) of the PET111 gene, the COX7 gene and a new gene (YMR255W) of unknown function that potentially codes for a polypeptide of 188 amino acids (about 21.5 kDa) without significant homology to any known protein. We have shown that the respiratory defect corresponding to group G199 is complemented by plasmids carrying only the COX7 gene. The gene YMR255W was inactivated by one-step gene replacement and the disrupted strain was viable and unaffected in its ability to grow in a variety of different test media such as minimal or complete media using eight distinct carbon sources at three pH values and temperatures. Inactivation of this gene also did not affect mating or sporulation

Chicago

Erip.: Kyläkirjasto.

Fingerprinting of cell lines by directed amplification of minisatellite-region DNA (DAMD)

The development of in vitro propagation of cells has been an extraordinary technical advance for several biological studies. The correct identification of the cell line used, however, is crucial, as a mistaken identity or the presence of another contaminating cell may lead to invalid and/or erroneous conclusions. We report here the application of a DNA fingerprinting procedure (directed amplification of minisatellite-region DNA), developed by Heath et al. [Nucleic Acids Research (1993) 21: 5782-5785], to the characterization of cell lines. Genomic DNA of cells in culture was extracted and amplified by PCR in the presence of VNTR core sequences, and the amplicons were separated by agarose gel electrophoresis. After image capture with a digital camera, the banding profiles obtained were analyzed using a software (AnaGel) specially developed for the storage and analysis of electrophoretic fingerprints. The fingerprints are useful for construction of a data base for identification of cell lines by comparison to reference profiles as well as comparison of similar lines from different sources and periodic follow-up of cells in culture.