Investigação da ação da proteína anexina A1 sobre o processo de angiogênese induzido pelo fator de crescimento do endotélio vascular: modelos experimentais in vivo e in vitro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tumor mamário canino: estudo in vitro, imunomarcação e ação da doxorrubicina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Diabetes reduces mesenchymal stem cells in fracture healing through a TNF alpha-mediated mechanism

Diabetes interferes with bone formation and impairs fracture healing, an important complication in humans and animal models. The aim of this study was to examine the impact of diabetes on mesenchymal stem cells (MSCs) during fracture repair.Fracture of the long bones was induced in a streptozotocin-induced type 1 diabetic mouse model with or without insulin or a specific TNF alpha inhibitor, pegsunercept. MSCs were detected with cluster designation-271 (also known as p75 neurotrophin receptor) or stem cell antigen-1 (Sca-1) antibodies in areas of new endochondral bone formation in the calluses. MSC apoptosis was measured by TUNEL assay and proliferation was measured by Ki67 antibody. In vitro apoptosis and proliferation were examined in C3H10T1/2 and human-bone-marrow-derived MSCs following transfection with FOXO1 small interfering (si)RNA.Diabetes significantly increased TNF alpha levels and reduced MSC numbers in new bone area. MSC numbers were restored to normal levels with insulin or pegsunercept treatment. Inhibition of TNF alpha significantly reduced MSC loss by increasing MSC proliferation and decreasing MSC apoptosis in diabetic animals, but had no effect on MSCs in normoglycaemic animals. In vitro experiments established that TNF alpha alone was sufficient to induce apoptosis and inhibit proliferation of MSCs. Furthermore, silencing forkhead box protein O1 (FOXO1) prevented TNF alpha-induced MSC apoptosis and reduced proliferation by regulating apoptotic and cell cycle genes.Diabetes-enhanced TNF alpha significantly reduced MSC numbers in new bone areas during fracture healing. Mechanistically, diabetes-enhanced TNF alpha reduced MSC proliferation and increased MSC apoptosis. Reducing the activity of TNF alpha in vivo may help to preserve endogenous MSCs and maximise regenerative potential in diabetic patients.

In vitro mucus transportability, cytogenotoxicity, and hematological changes as non-destructive physiological biomarkers in fish chronically exposed to metals

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The cosmetic dye quinoline yellow causes DNA damage in vitro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fibroblast growth factor 17 and bone morphogenetic protein 15 enhance cumulus expansion and improve quality of in vitro-produced embryos in cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Androgen receptors and experimental bone loss - an in vivo and in vitro study

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Protective effects of the galectin-1 protein on in vivo and in vitro models of ocular inflammation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Osteoblast response to porous titanium and biomimetic surface: In vitro analysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

In vitro embryos production after oocytes treatment with forskolin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Titanium-35niobium alloy as a potential material for biomedical implants: in vitro study

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Migração e invasão celular in vitro de células humanas expressando variantes da oncoproteína LMP1 do vírus de Epstein-Barr (EBV)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

In vitro reduction of Streptococcus mutans biofilm on silver nanoparticle-modified resin composite

Introduction: Currently, new methods to reduce biofilm formation on biomaterials are very studied, for example the use of silver nanoparticles, which were bactericidal. However, there are few studies investigating the benefits of these particles in dental restorative materials. Objective: This study aimed to compare in vitro the Streptococcus mutans biofilm formation on conventional light-cured composite resin with that on experimental light-cured composite resin, modified with silver nanoparticles. Material and methods: Discs were produced with either conventional resin (control group) and resin modified with different concentrations of silver nanoparticles, 0.1%, 0.3% and 0.6 % wt. (groups 1, 2 and 3, respectively). The samples were incubated in bacterial suspension (S. mutans) enriched with 20% sucrose to promote biofilm growth on the surfaces. Incubation times were 1, 4 and 7 days. After each period, adherent biofilms were disaggregated by ultrasound. Then, the numbers of viable cells recovered from the biofilms were counted through the serial dilution method. A morphological analysis of biofilm was also performed by Scanning Electron Microscopy. The data were subjected to Anova and Tukey’s test (α = 0.05). Results: The number of viable cells was statistically lower in groups 2 and 3 than in group 1 and control group, after the three incubation periods, without statistical difference between groups 2 and 3. The number of viable cells was statistically lower in group 1 than in control group, after 4 and 7 days of incubation. Conclusion: Resins modified with silver presented reduction of S. mutans biofilm on their surfaces, according to the conditions of this study.

In Vitro Safety Evaluation of Caffeic Acid

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção de progesterona pelas células luteínicas esteroidogênicas bovina cultivadas e co-cultivadas in vitro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)