988 resultados para 192-1185B
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The conformation of phenyl rings in the side groups of the helical chain polymer poly(tripenyl-methyl methacrylate) (1) in solution was studied by spectroscopic methods. According to the Raman spectrum the phenyl rings of 1 and triphenylmethyl methacrylate in solution have the same depolarization ratio at 1002 cm-1. The electronic spectra (ultraviolet and fluorescence) of 1 are similar to those of model substances, except for the "red shift" of the spectra of about 5 nm. It was concluded that the phenyl rings can rotate around the phenyl-C bond.
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(eta-5-C5H5)2YbCl.THF reacts with an equivalent molecular quantity of K(2,4-C7H11) (2,4-dimethyl pentadienyl potassium), and treatment of the product with DME yields (eta-5-C5H5)2Yb.DME. The crystal of (eta-5-C5H5)2Yb.DME belongs to the orthorhombic space group Fdd2 with a = 13.678(4) angstrom, b = 23.255(6) angstrom, c = 9.192(2) angstrom and Z = 8. The crystal parameters are found to differ from previously reported data.
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The toughening effect of the separate phases of ethylene/propylene block copolymers and their blends was studied by scanning electron microscopy (SEM). The results obtained show that the interfacial adhesion between separate phases and the isotactic polypropene (iPP) matrix in ethylene/propylene block copolymers is strong at room temperature, but poor at low temperature; specimens exhibit tearing of separate phases during fracture at room temperature, but interfacial fracture between separate phases and the iPP matrix at low temperature. From the characteristics of fractographs of ethylene/propylene block copolymers and their blends, it could be concluded that the separate phases improve the toughness of specimens in several ways: they promote the plastic deformation of the iPP, and they can be deformed and fractured themselves during the fracture process. However, it was shown that the plastic deformation processes, such as multiple-crazing, shear yielding, etc. of the matrix are the dominant mechanisms of energy absorption in highly toughened ethylene/propylene block copolymers and their blends. The deformation and fracture of separate phases are only of secondary importance.
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The feasibility of applying the method of factor analysis to X-ray diffraction diagrams of binary blends of polypropylene and ethylene-propylene-diene terpolymer (PP/EPDM) was examined. The result of mathematical treatment was satisfactory. The number of scattering species and their concentrations in six kinds of PP/EPDM blends were determined. The separation of the spectral peaks of each species in the blends, contributing spectral intensities, was carried out.
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用透射电子显微镜观察了高密度辐照聚乙烯的形态结构,并通过统计方法定量地分析了其结构与辐照剂量的关系。发现室温辐照聚乙烯的片晶形态不随辐照剂量而变化。若将室温辐照聚乙烯重新熔融,然后再于125℃下等温结晶4h后,其片晶厚度则随辐照剂量的增加而变薄,长周期亦随之变短。小角X射线散射的测试结果与上述结果符合得很好。室温辐照聚乙烯及其125℃重结晶试样的电子显微镜数据从又一直观角度验证了辐照聚乙烯“片晶内部破坏机理”的正确性。
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本文研究了聚合溶液中酸浓度对苯胺的化学聚合行为及所得产物导电性能的影响。并用FTIR、元素分析及XPS等手段表征了不同条件下获得的聚合物分子链结构。
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A new rearranged chamigrane sesquiterpene, named laurenokamurin, was isolated from the marine red alga Laurencia okamurai. Its structure was determined on the basis of spectroscopic methods. (C) 2008 Bin Gui Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
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In invertebrates, C-type lectins play crucial roles in innate immunity responses by mediating the recognition of host cells to pathogens and clearing microinvaders, which interact with carbohydrates and function as pattern recognition receptors (PRRs). A novel C-type lectin gene (LvLec) cDNA was cloned from hemocytes of Litopenaeus vannamei by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of LvLec was of 618 bp, consisting of a 5'-terminal untranslated region (UTR) of 60 bp and a 3'-UTR of 87 bp with a poly (A) tail. The deduced amino acid sequence of LvLec possessed all conserved features critical for the fundamental structure, such as the four cysteine residues (Cys(53), Cys(128), Cys(144), Cys(152)) involved in the formation of disulfides bridges and the potential Ca2+/carbohydrate-binding sites. The high similarity and the close phylogenetic relationship of LvLec shared with C-type lectins from vertebrates and invertebrates. The structural features of LvLec indicated that it was an invertebrate counterpart of the C-type lectin family. The cDNA fragment encoding the mature peptide of LvLec was recombined and expressed in Escherichia coli BL21(DE3)-pLysS. The recombinant protein (rLvLec) could agglutinate bacteria E. coli JM109 depending on Ca2+, and the agglutination could be inhibited by mannose and EDTA. These results indicated that LvLec was a new member of C-type lectin family and involved in the immune defence response to Gram negative bacteria in Litopenaeus vannamei. (C) 2008 Elsevier Ltd. All rights reserved.
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Cyanobacteria are the oldest life form making important contributions to global CO2 fixation on the Earth. Phycobilisomes (PBSs) are the major light harvesting systems of most cyanobacteria species. Recent availability of the whole genome database of cyanobacteria provides us a global and further view on the complex structural PBSs. A PBSs linker family is crucial in structure and function of major light-harvesting PBSs complexes. Linker polypeptides are considered to have the same ancestor with other phycobiliproteins (PBPs), and might have been diverged and evolved under particularly selective forces together. In this paper, a total of 192 putative linkers including 167 putative PBSs-associated linker genes and 25 Ferredoxin-NADP oxidoreductase (FNR) genes were detected through whole genome analysis of all 25 cyanobacterial genomes (20 finished and 5 in draft state). We compared the PBSs linker family of cyanobacteria in terms of gene structure, chromosome location, conservation domain, and polymorphic variants, and discussed the features and functions of the PBSs linker family. Most of PBSs-associated linkers in PBSs linker family are assembled into gene clusters with PBPs. A phylogenetic analysis based on protein data demonstrates a possibility of six classes of the linker family in cyanobacteria. Emergence, divergence, and disappearance of PBSs linkers among cyanobacterial species were due to speciation, gene duplication, gene transfer, or gene loss, and acclimation to various environmental selective pressures especially light.
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In order to investigate the possible effects of the ecological environment on photosynthetic activity and the major light harvesting complex, the oxygen evolution rates and composition of phycobilisome from marine red alga Porphyra yezoensis Ueda and freshwater red alga Compsopogon coeruleus (Balbis) Montagne, which could grow and reproduce under salinity up to 35 ppt, were studied. The results showed that the oxygen evolution rate of P. yezoensis in seawater was significantly higher than that of C. coeruleus in freshwater, and P. yezoensis tolerated inorganic ions at a relatively higher concentration than C. coeruleus. Moreover, the phycoerythrin (PE) of P yezoensis was R-phycoerythrin containing alpha, beta, and gamma subunits comprised phycoerythrobilin and phycourobilin. In contrast, the PE from C. coeruleus consisted of alpha, beta, and gamma subunits comprised only phycoerythrobilin but not phycourobilin, suggesting that the PE from C. coeruleus was of a new type.
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Diatom data of 192 surface sediment samples from the marginal seas in the western Pacific together with modern summer and winter sea surface temperature and salinity data were analyzed. The results of canonical correspondence analysis show that summer sea-surface salinity (SSS) is highly positively correlated with winter SSS and so is summer sea-surface temperature (SST) with winter SST. The correlations between SSSs and SSTs are less positively correlated, which may be due to interactions of regional current pattern and monsoon climate. The correlations between diatom species, sample sites and environmental variables concur with known diatom ecology and regional oceanographic characters. The results of forward selection of the environmental variables and associated Monte Carlo permutation tests of the statistical significance of each variable suggest that summer SSS and winter SST are the main environmental factors affecting the diatom distribution in the area and therefore preserved diatom data from down core could be used for reconstructions of summer SSS and winter SST in the region.
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Based on high-resolution 3D seismic data, we document the polygonal faults within the Miocene Meishan (sic) Formation and Huangliu (sic) Formation of the Qiongdongnan (sic) basin, northern South China Sea. Within the seismic section and time coherent slice, densely distributed extensional faults with small throw and polygonal shape were identified in map view. The orientation of the polygonal faults is almost isotropic, indicating a non-tectonic origin. The deformation is clearly layer-bounded, with horizontal extension of 11.2% to 16%, and 13.2% on average. The distribution of polygonal faults shows a negative correlation with that of gas chimneys. The development of polygonal faults may be triggered by over-pressure pore fluid which is restricted in the fine-grained sediments of bathyal facies when the sediments is compacted by the burden above. The polygonal faults developed to balance the volumetric contraction and restricted extension. The product of hydrocarbon in the Meishan Formation may have contributed to the development of the polygonal faults. In the study area, it was thought that the petroleum system of the Neogene post-rift sequence is disadvantageous because of poor migration pathway. However, the discovery of polygonal faults in the Miocene strata, which may play an important role on the fluid migration, may change this view. A new model of the petroleum system for the study area is proposed.
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浮游动物作为海洋食物链中承上启下的一环,其群落结构和动态变化直接影响着海洋生态系统能流、物流的方向和效率。胶州湾作为一个受自然环境和人类活动双重影响的海湾,是研究人为活动对自然生态系统影响的模式港湾。因此,深入研究胶州湾浮游动物的群落特征及时空分布特点对了解该海湾生态系统的现状与历史变化趋势以及生态系统对自然条件变化和人类干扰的响应具有重要意义,也可为其他海区的研究提供参考资料。 本文根据2004年1月-12月用浅水I型和浅水II型浮游生物网在胶州湾每月一次的垂直拖网采集的浮游动物样品,分析了胶州湾浮游动物的物种组成和优势种的时空分布特征。2004年胶州湾浮游动物共74个种,分属于暖温带近岸种和暖温带近海种。浮游动物丰度周年波动范围1006-15571 ind./m3,全年平均5187 ind./m3。在胶州湾体长<1mm的浮游动物丰度和生物量最大。浮游动物的丰度和物种多样性季节变化明显,春夏大(高),秋冬小(低)。多数月份浮游动物丰度在湾内大于湾外。物种多样性在湾北和湾南较低,在湾中心和湾外较高。 胶州湾浮游动物优势种为双刺纺锤水蚤、小拟哲水蚤、太平洋纺锤水蚤、中华哲水蚤、拟长腹剑水蚤、近缘大眼剑水蚤、长尾住囊虫、强壮箭虫、短角长腹剑水蚤、八斑芮氏水母和小介穗水母等。双刺纺锤水蚤全年都是优势种,小拟哲水蚤除了4月其余月份都是优势种。其他优势种季节更替明显,冬、春季(12月、1-5月)优势种为中华哲水蚤、拟长腹剑水蚤、近缘大眼剑水蚤、长尾住囊虫等;夏季(6-8月)太平洋纺锤水蚤代替中华哲水蚤、拟长腹剑水蚤成为优势种;秋季(9-11月)优势种更替频繁,9月为短角长腹剑水蚤和太平洋纺锤水蚤,10月为太平洋纺锤水蚤、拟长腹剑水蚤和长尾住囊虫,11月为拟长腹剑水蚤、长尾住囊虫、近缘大眼剑水蚤和强壮箭虫。 双刺纺锤水蚤与中华哲水蚤在4月达到数量高峰,丰度分别为5604ind./m3和493 ind./m3。长尾住囊虫6月丰度1809 ind./m3为全年最大。小拟哲水蚤、太平洋纺锤水蚤和近缘大眼剑水蚤在7月达到数量高峰,丰度分别为1667 ind./m3、4000 ind./m3、775 ind./m3。短角长腹剑水蚤和强壮箭虫在8月丰度最大,分别为389ind./m3和192 ind./m3。八斑芮氏水母和小介穗水母分别在3、8月大量出现,丰度分别为194 ind./m3、358 ind./m3。拟长腹剑水蚤全年有7、10月两个高峰。双刺纺锤水蚤、小拟哲水蚤、太平洋纺锤水蚤、短角长腹剑水蚤、八斑芮氏水母和小介穗水母主要在湾北密集;中华哲水蚤密集区趋向于湾中心和湾外;近缘大眼剑水蚤、拟长腹剑水蚤、异体住囊虫和强壮箭虫等分布广泛,不同月份密集区不同。 从目前我们所掌握的资料来看,近30年来,胶州湾浮游动物的种类组成和优势种群没有发生大的变化。与70年代相比,2004年的物种数基本不变,但物种组成稍有不同;优势种丰度大大降低。与90年代相比,2004年物种数有所增加,其中水母类增加了15种,八斑芮氏水母成为冬季优势种;浮游动物的丰度和优势种的水平分布格局基本不变。胶州湾浮游动物的生态类型与渤海相似,但与黄海有所差异;胶州湾优势种的丰度大于渤海,且高峰期比渤海提前一个月。
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趋磁细菌(Magnetotactic bacteria)的研究是国际微生物学研究热点之一。趋磁细菌体内含有纳米单磁畴的氧化铁/硫化铁(Fe3O4或Fe3S4)晶体,称为磁小体。由于趋磁细菌营养条件要求苛刻,在环境中需要微好氧条件,且营养类型属于化能自养,使得培养趋磁细菌时常遇到问题。 本研究首先通过正交试验优化趋磁细菌AMB-1菌株培养条件,在培养条件铁源为奎尼酸铁0.02 mmol/L,装瓶量75% ,pH值6.7,温度25 ℃时,AMB-1 OD600达到0.440(1.166×109 cells/ml)。同时运用磁收集传代法,使带有磁小体的AMB-1细胞比例占95%以上(Cmag值稳定在1.9-2.0)。 在AMB-1具有较好的生物量,同时又具有较好的含磁小体细胞比例后,研究磁小体的变化过程。通过透射电镜观察磁小体变化过程,发现培养24 h细菌体内已有较小晶体形成(平均27 nm,n=188)且沿长轴分布;48 h晶体长大(平均43 nm,n=203)且形成分段链沿长轴排列;72 h晶体进一步成熟(平均50 nm,n=191)仍以分段链沿长轴排列;随后细菌逐渐衰亡磁小体变小,168 h可见部分自溶细菌中仍有磁小体链(平均37 nm,n=186);192 h细菌自溶磁小体链(平均33 nm,n=184)分散到环境中。 通过透射电镜在细胞水平上研究趋磁细菌细胞分裂时发现,磁小体在细菌分裂时采用两种分离方式:一种为磁小体分配到两个子细胞;另一种为磁小体只分配到一个子细胞。无磁小体的子细胞,在随后的生长过程又分为两种情况:一种为细胞逐渐产生磁小体,另一种为不再产生磁小体。这种现象的发现,解释了随着传代次数的增多,细菌磁性有所下降的原因(Cmag值降低)。 在对趋磁细菌磁小体合成机制的研究中,常使用基因敲除的办法获得缺陷型,并与野生型对比进行研究。但是,利用基因敲除获得缺陷型不仅操作繁琐并且所得缺陷型不稳定。本研究利用特殊的磁富集传代法,先将带有磁小体的菌体收集并连续传代,筛选获得了高磁菌株;利用这种方法,收集不含磁小体的菌体并连续传代,筛选获得了无磁菌株。 趋磁细菌磁小体在医疗、环保等领域具有广阔应用价值,但是目前由于趋磁细菌难以大规模培养,并且磁小体纯化存在成本高等原因,将磁小体真正实际应用尚有一段距离。通过研究磁小体在趋磁细菌中的变化过程发现,AMB-1菌株在培养192 h后自溶,并且磁小体随着细胞的破碎释放到环境中去。
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能量代谢指动物在进行生理活动(如摄食、消化以及动物的活动等)时所消耗能量的总和,一般以动物的呼吸率利排泄率来估计动物的能量代谢。其主要研究内容是闸明生物能量代谢的基木规律以及与环境闪子的关系。菲律宾蛤仔(Ruditapesphil ippmarum)是我国一种重要的养殖贝类,关于其能量代谢的研究却较少,这种状况妨碍了菲律宾蛤仔养殖生态理论的完善和养殖技术的提高。本研究主要对菲律宾蛤仔呼吸率和排泄率的基本规律(能量代谢与体重的关系、能量代谢的昼夜变化)及其与环境因子(饵料浓度、水温、栖息底质环境)的关系进行探讨。研究结果如下:1.不同体重菲律宾蛤仔代谢率小同。实验川菲律宾蛤仔分三种大小:l(干肉重为0.07-0.14g)、ll(干肉重0.27-0.34g)、III(干肉重0.45~0.63g)。温度包括:26℃(八月)、20℃(十月)、1 5℃(十二月)、9℃(一月)。实验共设四个饵料浓度:2.28±0.25,6.454±0.44,10.284±0.82,15.414±1.56mgTPM/L(TPM,总颗粒物),饵料中POM(颗粒有机物)含量都为4.68±1.64 mg/L。常温下菲律宾蛤仔代谢率随着体重的增大而增大。15℃、20~C、26℃时蛤仔呼吸率与干肉重呈明显的幂函数关系R=aW~b,a值变动范围为0.1076-0.3309;b值变动范围为0.239l~0.8381;蛤仔排泄率与干肉重也呈明显的幂函数关系N=aW~b,a值变动范围为14.213~68.362:b值变动范围为0.3673-1.1 532。9℃(饵料浓度为2.28±0.25mgTPM/L)、20℃(饵料浓度为10.284-0.82mgTPM/L)、26℃(饵料浓度为6.454±0.44mgTPM/L)时不同体重蛤仔氧氮比差异显著,其它情况下不同体重蛤仔氧氮比差异不显著。2.常温下菲律宾蛤仔代谢率受饵料浓度的影响,不同大小蛤仔受饵料浓度的影响程度不同。I组蛤仔呼吸率受饵料浓度的显著影响,II组III组蛤仔呼吸率只在9℃(一月)和26~C(八月)时受饵料浓度的显著影响。26℃时影响最显著,26℃时I组蛤仔在饵料浓度为2.28±0.25,6.45±0.44,l0.28±0.82,15.4l±1.56mgTPM/L时呼吸率分别是O.086,0.146,0.073,0.093(mlO_2/h);ll组蛤仔在上述浓度饵料中呼吸率分别是0.138,0.214,0.J 26,0.12l(mlO_2/h);III组蛤仔在上述浓度饵料中呼吸率分别是0.129,0.266,0.186,0.192(mlO_2/h)。菲律宾蛤仔呼吸率在饵料浓度为6.45±0.44 mgTPM/L时最高,蛤仔呼吸率在其它饵料浓度时都会降低。菲律宾蛤仔排泄率在饵料浓度为10.28±0.82 mgTPM/L和15.4l士1.56mgTPM/L时显著高于其它浓度组,9℃时这种趋势更明显,9℃时饵料浓度为2.28±0.25,6.454±044,lO.284±0.82,15.41±1.56mgTPM/L中I组蛤仔排泄率分别是4.297,2.874,8.003,6.658(μgNH_3-N/h);II组蛤仔在上述浓度饵料中排泄率分别是4.011,3.609,10.427,12.732(μgNH_3-N/h);III组蛤仔在上述浓度饵料中排泄率分别是2.28 l,6.452,10.283,15.417(μgNH_3-N/h)。3.菲律宾蛤仔代谢率受自然温度的显著影Ⅱ向。I组蛤仔在9℃、15℃、20℃、26℃时呼吸率平均为0.057,0.085,0.039,O.099;II组蛤仔在上述四个温度中呼吸率平均为0.08,O.128,0.089,0.149(mlO_2/h),I组和II组蛤仔在9℃和20~C时呼吸率较低,在26℃时呼吸率最高。III组蛤仔在上述四个温度中呼吸率平均为0.09,O.1 59,O.143,O.193(mlO_2/h),在9℃时llI组蛤仔呼吸率显著低于其它温度组。温度为9℃、15℃、20℃、26℃时l组蛤仔排泄率平均为5.458,13.169,4.946,11.138(μgNH_3-N/h):II组蛤仔在上述温度中排泄率平均为7.695,23.578,8.319,23.90l(μgNH_3-N/h);III组蛤仔在上述温度中排泄率平均为11.738,27.443,15.658,35.407(μgNH_3-N/h),蛤仔排泄率在15℃和26℃时均高于9℃和20℃。4.摄食状态与饥饿状态菲律宾蛤仔代谢率有明显不同。26℃时蛤仔静止状态呼吸率平均为0.336(m102/g干重.h),摄食状态呼吸率平均为0.656(ml0_2干重.h),摄食状态呼吸率比静止状态平均升高了0 32(ml0_2/g干重.h);26℃时蛤仔静止状态排泄率平均为39.471(μgNH_3-N/g干重.h),摄食状态排泄率平均为88.08(μgNH_3-N/g干重.h),摄食状态排泄率比静止状态排泄率平均升高了48.6(μgNH_3-N/g干重.h)。摄食状态代谢率平均是静止状态的2~3倍。根据摄食引起的呼吸率和排泄率升高量得出每氧化产生lμgNH_3-N需0_2量平均为7.05μl。5.人工控制温度对菲律宾蛤仔代谢率有明显影响。不同大小蛤仔受温度的影响程度不同。在温度5℃、10℃、l 5℃、20℃、26℃,I组和II组蛤仔呼吸率都随着温度的升高而升高,在10℃~l5℃和20℃~26℃这二个温度变化范围内呼吸率变化最大,在20℃~26℃时I组蛤仔呼吸率变动范围为O.85~1.04(m10_2/g干重.h)、II组蛤仔变动范围为0.57~0.86(ml0_2/g干重.h)。III组蛤仔呼吸率只在5℃~l0℃时明显增高,变动范围为0.09~0.5l(m10_2/g干重.h),在10℃~26℃范围内变化不大。I组和II组蛤仔排泄率随着温度的升高而升高,变动幅度较大,在5℃~26℃范围内其排泄率变动范围为10.32~81.53(μgNH_3-N/g干重.h);而 III组蛤仔排泄率只在5℃~15℃时随着温度的升高而升高,其排泄率变动范围为6.75~23.77(μgNH_3-N/g干重.h),在15℃~26℃范围内几乎不变。III组蛤仔的适温范围比I组和II组蛤仔广。菲律宾蛤仔在5℃和10℃时氧氮比变化明显,变动范围为2.76~11.44,在15~26℃时变化不大。6.菲律宾蛤仔代谢率有明显的日节律性,呈正弦曲线型变化。蛤仔夜问代谢率明显升高。I组蛤仔夜间呼吸率平均为0.867(m10_2/g干重.h),白天呼吸率平均为O.504(m10_2/g干重.h);II组蛤仔夜间呼吸率平均为0.438(m10_2/g干重.h),白天呼吸率平均为0.36l(m102/g干重.h);III组蛤仔夜间呼吸率平均为0.409(m10_2/g干重.h),白天呼吸率平均为0.252(m102/g干重.h)。在22:00-23:00菲律宾蛤仔呼吸率最高。7.底质环境对菲律宾蛤仔的代谢率有明显影响。在饥饿状态下菲律宾蛤仔在泥沙底质中呼吸率平均为l 406(m10_2/g干重h),在无泥沙环境中呼吸率平均为O.963(ml0_2/g干重.h);摄食状态下菲律宾蛤仔在泥沙底质中呼吸率平均为1.59l(m102/g干重.h),在无泥沙环境中呼吸率平均为1.115(m10_2/g干重.h)。在饥饿状态下菲律宾蛤仔在泥沙底质中排泄率平均为78.934(μgNH_3-N/g 干重.h),在无泥沙环境巾排泄率平均为45.043(μgNH_3-N/g干重.h);摄食状态下菲律宾蛤仔在泥沙底质中排泄率平均为87.12l(μgNH_3-N/g干重.h),在无泥沙底质中排泄率平均为58.354(μgNH_3-N/g干重.h)。蛤仔在泥沙环境中呼吸率和排泄率都明显升高。