Tourist inns and communications 1939

kuv., 10 x 22 cm

Tourist inns and communications 1938

kuv., 10 x 20 cm

Tourist inns and communications 1937

kuv., 10 x 20 cm

Role of the canonical transient receptor potential channel 2 (TRPC2) in thyroid cell function

Kalciumjonen reglerar flera processer i celler såsom transkribering av gener, celldelning, cellernas rörlighet och celldöd. Därför har cellerna utvecklat många mekanismer för att reglera den intracellulära kalciumkoncentrationen. Kalciumkanaler spelar en viktig roll i denna regleringsprocess. TRPC-kanalerna (eng. canonical transient receptor potential) är en familj av jonkanaler med sju medlemmar (TRPC1-7) vars regleringsmekanismer och fysiologiska roller är varierande. TRPC2-kanalens fysiologiska signifikans, samt hur kanalen regleras, är dåligt karakteriserad. För första gången, rapporterar vi närvaron av TRPC2 kanalen i råttans sköldkörtelceller samt primära sköldkörtelceller från råtta. Hos gnagare har TRPC2 antagits vara exklusivt uttryckt i det vomeronasala organet. För att undersöka den fysiologiska betydelsen av kanalen, har vi utvecklat stabila celler med nedreglerat TRPC2 (shTRPC2) m.h.a. shRNA-teknik. Nedreglering av TRPC2 resulterade i stora skillnader i flera viktiga cellulära funktioner och i regleringen av sköldkörtelcellernas cellsignalering. Nedreglering av TRPC2 orsakade minskad agonist-beroende frigivning av kalcium från det endoplasmatiska nätverket, samt minskat agonist-beroende inflöde av extracellulärt kalcium, men ökade det basala kalciuminflödet. Uttrycket av PKCβ1 och PKCδ, SERCA-aktiviteten och kalciumhalten i det endoplasmatiska nätverket minskade i shTRPC2 celler. Kommunikation mellan kalcium- och cAMP-signalering påvisades vara TRPC2-beroende, vilket visades reglera uttrycket av TSH-receptorn. Vi undersökte också betydelsen av TRPC2 kanalen i reglering av sköldkörtelcellers proliferation, migration, vidhäftning och invasion; processer som alla var dämpade i shTRPC2 celler. Samamnfattningsvis påvisade dessa resultat en ny och viktig fysiologisk betydelse för TRPC2 kanalerna.

The marketing communications industry in flux: Large B2C advertisers’ viewpoint

The recent digitization, fragmentation of the media landscape and consumers’ changing media behavior are all changes that have had drastic effects on creating marketing communications. In order to create effective marketing communications large advertisers are now co-operating with a variety of marketing communications companies. The purpose of the study is to understand how advertisers perceive these different companies and more importantly how do advertisers expect their roles to change in the future as the media landscape continues to evolve. Especially the changing roles of advertising agencies and media agencies are examined as they are at the moment the most relevant partners of the advertisers. However, the research is conducted from a network perspective rather than focusing on single actors of the marketing communications industry network. The research was conducted using a qualitative theme interview method. The empirical data was gathered by interviewing representatives from nine of the 50 largest Finnish advertisers measured by media spending. Thus, the research was conducted solely from large B2C advertisers’ perspective while the views of their other relevant actors of the network were left unexplored. The interviewees were chosen with a focus on variety of points of view. The analytical framework that was used to analyze the gathered data was built the IMP group’s industrial network model that consists of actors, their resources and activities. As technology driven media landscape fragmentation and consumers’ changing media behavior continue to increase the complexity of creating marketing communications, advertisers are going to need to rely on a growing number of partnerships as they see that the current actors of the network will not be able to widen their expertise to answer to these new needs. The advertisers expect to form new partnerships with actors that are more specialized and able to react and produce activities more quickly than at the moment. Thus, new smaller and more agile actors with looser structures are going to appear to fill these new needs. Therefore, the need of co-operation between the actors is going to become more important. These changes pose the biggest threat for traditional advertising agencies as they were seen as being most unable to cope with the ongoing change. Media agencies are in a more favorable position for remaining relevant for the advertisers as they will be able to justify their activities and provided value by leveraging their data handling abilities. In general the advertisers expect to be working with a limited number of close actors and in addition having a network of smaller actors, which are used on a more ad hoc basis.

Análise interactômica da VDAC (voltage-dependent anion selective channel) nos cérebros aviar, bovino e murino

A VDAC é a proteína mais abundante na membrana mitocondrial externa. Exerce o controle da atividade desta organela através da regulação da troca de metabólitos e tem função crucial no mecanismo de apoptose. Em nosso caso, os estudos dos complexos protéicos, das interações entre a VDAC e outras proteínas presentes no interior do neurônio que auxiliam na manutenção das funções das organelas e da célula, fazem parte da chamada interactômica. O presente estudo determinou o interactoma do complexo protéico Hexoquinase-VDAC-ANT presente em cérebros murino, bovino e aviar. Nosso objetivo foi identificar se as expressões diferenciadas da VDAC1 e VDAC2 verificadas nos cérebros murino, aviar e bovino, estão associadas a diferenças nos interactomas dessas proteínas. Este estudo revelou que as espécies aviar e bovina apresentaram o maior número de complexos protéicos contendo VDACs (5) quando comparadas com os neurônios de rato (1), o que é indicativo de uma cinética diferencial de montagem ou desmontagem do complexo. Além disso, a VDAC mitocondrial neuronal aviar também interage com mais proteínas em relação à VDAC mitocondrial neuronal bovina, o que é resultado de uma composição de subunidades diferenciada. Tais resultados indicam diferenças significativas quanto ao metabolismo energético e apoptótico no cérebro aviar, bovino e murino, existindo interações diferenciais da VDAC no cérebro aviar.

Tunable lumped-element bandpass filters for Cognitive Radio application

In this thesis the design of bandpass filters tunable at 400 MHz – 800 MHz was under research. Microwave filters are vital components which provide frequency selectivity in wide variety of electronic systems operating at high frequencies. Due to the occurrence of multi-frequency bands communication and diverse applications of wireless devices, requirement of tunable filters exists. The one of potential implementation of frequency-agile filters is frontends and spectrum sensors in Cognitive Radio (CR). The principle of CR is to detect and operate at a particular available spectrum without interfering with the primary user’s signals. This new method allows improving the efficiency of utilizing allocated spectrum such as TV band (400 MHz – 800 MHz). The focus of this work is development of sufficiently compact, low cost tunable filters with quite narrow bandwidth using currently available lumped-element components and PCB board technology. Filter design, different topologies and methods of tuning of bandpass filters are considered in this work. As a result, three types of topologies of bandpass filter were simulated and realised. They use digitally tunable capacitors (DTCs) for adjusting central frequency at TV "white space" spectrum. Measurements revealed that schematics presented in this work have proper output response and filters are successfully tuned by DTCs.

KAVA:n radio- ja tv-arkisto : ohjelmavirtaa ja talletuksia tutkimuskäyttöön

Teemanumero 1/2012: Arkisto.

Sähköisen markkinoinnin markkinointisuunnitelma metallialan pk-yrityksen kansainvälistymiseen Venäjälle

Suuri osa ihmisten tiedon etsinnästä painottuu internetiin ja siellä käytettäviin hakukoneisiin. Myös yritykset ovat tiedostaneet sähköisen markkinoinnin roolin omassa kansainvälisessä näkyvyydessään. Pienyritysten kansainvälistymisessä sähköisellä markkinoinnilla voidaan parantaa markkinatiedon etsintää, asiakkaiden tavoitettavuutta ja markkinointiviestinnän kohdennettavuutta eri käyttäjäryhmille. Sähköinen markkinointi ei kuitenkaan poista kulttuurisidonnaisia epävarmuuksia, ja sen laadukas toteuttaminen vaatii perehtymistä. Tutkimuksen tavoitteena on selvittää Case-yritykselle sähköisen markkinoinnin potentiaaliset hyödyntämisvaihtoehdot kansainvälistymisprosessissa Venäjälle ja suunnitella resursseihin nähden toteutettava sähköisen markkinoinnin markkinointisuunnitelma. Monimenetelmätekniikalla toteutetussa tutkimuksessa tietolähteenä käytetään asiantuntijahaastatteluja sekä datarekistereitä. Teoriapohjana tarkastellaan pk-yrityksien tiedon vaiheittaista oppimista, verkoston roolia sekä ulkoistamispäätöksen tukena käytettyjä transaktiokustannuksia. Sähköisen markkinoinnin kehitys on ollut Venäjällä kovaa viimeisen kahden vuoden aikana. Tästä syystä venäläisten yritysten sähköisen markkinoinnin taso on osittain korkeampaa kuin suomalaisten. Sähköisellä markkinoinnilla tavoittaa erityisesti kaupungeissa asuvat ihmiset, mutta kaupunkien ulkopuolella verkkotekniikka on vielä osittain alkeellisella tasolla. Vientimuotojen vertailussa transaktiokustannusten avulla nousi esille suoran viennin malli, jossa jälleenmyyjän etsimisen avuksi kehitetään sähköisen markkinoinnin osaamista. Paikallisella jälleenmyyjällä nähdään olevan etuja sähköisen markkinoinnin, paikallistuntemuksen ja kulttuurillisten erityispiirteiden tuntemuksessa.

Power-line-communication-based data transmission concept for an LVDC electricity distribution network – Analysis and implementation

Communications play a key role in modern smart grids. New functionalities that make the grids ‘smart’ require the communication network to function properly. Data transmission between intelligent electric devices (IEDs) in the rectifier and the customer-end inverters (CEIs) used for power conversion is also required in the smart grid concept of the low-voltage direct current (LVDC) distribution network. Smart grid applications, such as smart metering, demand side management (DSM), and grid protection applied with communications are all installed in the LVDC system. Thus, besides remote connection to the databases of the grid operators, a local communication network in the LVDC network is needed. One solution applied to implement the communication medium in power distribution grids is power line communication (PLC). There are power cables in the distribution grids, and hence, they may be applied as a communication channel for the distribution-level data. This doctoral thesis proposes an IP-based high-frequency (HF) band PLC data transmission concept for the LVDC network. A general method to implement the Ethernet-based PLC concept between the public distribution rectifier and the customerend inverters in the LVDC grid is introduced. Low-voltage cables are studied as the communication channel in the frequency band of 100 kHz–30 MHz. The communication channel characteristics and the noise in the channel are described. All individual components in the channel are presented in detail, and a channel model, comprising models for each channel component is developed and verified by measurements. The channel noise is also studied by measurements. Theoretical signalto- noise ratio (SNR) and channel capacity analyses and practical data transmission tests are carried out to evaluate the applicability of the PLC concept against the requirements set by the smart grid applications in the LVDC system. The main results concerning the applicability of the PLC concept and its limitations are presented, and suggestion for future research proposed.

Managing Customer Issues through a Support Channel Network

The importance of after-sales service or service in general can be seen and experienced by customers every day with industrial as well as other non-industrial services or products. This dissertation, drawing on theory and experience, focuses on practical engineering implications, specifically the management of customer issues in the after-sales phase in the mobile phone arena. The main objective of this doctoral dissertation is to investigate customer after-sales issue management, specifically regarding mobile phones. The case studies focus on issue resolution time and the issue of corrective actions. This dissertation consists of a main body and four peer-reviewed journal articles and one manuscript currently under review by a peer-reviewed journal. The main body of this dissertation examines the elements of customer satisfaction, loyalty, and retention with respect to corrective actions to address customer issues and issue resolution time through literature and empirical studies. The five independent works are case studies supporting the thesis research questions. This study examines four questions: 1) What are the factors affecting corrective actions for customers? 2) How can customer issue resolution time be controlled? 3) What are the factors affecting processes in the service chain? and 4) How can communication be measured in a service chain? In this work, both quantitative and qualitative analysis methods are used. The main body of the thesis reviews the literature regarding the elements that bridge the five case studies. The case studies of the articles and surveys lean more toward the methodology of critical positivism and then apply the interpretive approach in interpreting the results. The case study articles employ various statistical methods to analyze and to interpret the empirical and survey data. The statistical methods were used to create a model that is useful for significantly optimizing issue resolution time. Moreover, it was found that samples for verifying issues provided by the customer neither improve the perceived quality of corrective actions nor the perceived quality of issue resolution time. The term “service” in this work is limited to the technical services that are provided by product manufacturers and after-sales authorized service vendors. On the basis of this research work, it has been observed that corrective actions and issue resolution time are associated with customer satisfaction and hence, according to induction theory, to customer loyalty and retention. This thesis utilizes knowledge of marketing and customer relationships to contribute to the existing body of knowledge concerning information and communication technology for after-sales service recovery of mobile terminals. The established models in the thesis contribute to the existing knowledge of the after-sales process of dealing with customer issues in the field of mobile phones. The findings suggest that process managers could focus more on communication and training provided to the staff as new technology evolves rapidly. The study also suggest the managers formulate strategies for how customers can be kept informed on a regular basis of the status of issues that have been escalated for corrective action. The findings also lay the foundation for the comprehensive objective to control the entire product development process, starting with conceptualization. This implies that robust design should be applied to the new products so that problems affecting customer service quality are not repeated. The objective will be achieved when the entire service chain from product development to the final user can be modeled and this model can be used to support the organization at all levels.

Kognitiivinen radio sotilaallisessa käyttöympäristössä

Tutkielmassa arvioidaan kognitiivisen radion sotilaalliseen ympäristöön tuomia mahdollisuuksia. Radion kognitiivisuutta käsitellään kongitiivisten ominaisuuksien, kuten adaptiivisuuden tai tilannetietoisuuden, kautta. Painopisteenä on kognitiivisten ominaisuuksien tuomien vaikutusten tarkastelu operatiivisessa viitekehyksessä. Operatiivisia vaikutuksia tutkitaan uhkalähtöisesti eli arvioimalla, miten tietty kognitiivinen ominaisuus vaikuttaa yleisimpiin tiedonsiirtoverkon uhkiin. Tutkimus on kirjallisuustutkimus, jota on täydennetty asiantuntijahaastatteluin sekä -arvioin. Operatiiviset vaikutukset on tiivistetty taulukoimalla eri kognitiivisten ominaisuuksien ja yleisimpien tiedonsiirtojärjestelmien uhkat. Kognitiiviselle radiolle on useita erilaisia määritelmiä. Tutkielman perusteella ITU-R:n määritelmää voidaan pitää kattavana. Kaikkia kriteerejä täyttävää kognitiivista radiota ei ole vielä olemassa, vaikka kognitiivisia ominaisuuksia radioissa on ollut pitkään. Kognitiivinen radio, tai jo pelkästään ohjelmistoradio kehittyneillä kognitiivisilla ominaisuuksilla, tuo huomattavia mahdollisuuksia parantaa langattoman tiedonsiirron toimintakykyä sotilaallisessa käyttöympäristössä. Kognitiiviset ominaisuudet vaikuttavat merkittävästi niin viestitaktiikkaan kuin uhkaankin. Tiedonsiirtojärjestelmien ylläpito ja rakentaminen vaativat aiempaa enemmän suunnittelua ja ammattitaitoa, mutta toisaalta loppukäyttäjän osaamistasovaatimuksia voidaan laskea sitä mukaan, kun lähestytään 0- konfiguraatiojärjestelmiä. Radioiden kognitiivisuuden myötä voidaan myös passiivisiin uhkiin, esimerkiksi elektroniseen tiedusteluun, varautua aiempaa tehokkaammin. Aktiivisiin uhkiin, kuten häirintään, kognitiiviset ominaisuudet vaikuttavat jopa toiminnan fundamentteja muuttavasti: perinteiset häirintätehtävät voivat vaihtua esimerkiksi tiedustelua tukeviksi tehtäviksi. Kognitiivisia ominaisuuksia voi olla myös häirintäjärjestelmissä, mikä toisaalta tasapainottaa mutta toisaalta vaikeuttaa häirinnän vaikutuksen ennakointia ja arviointia. Tiedonsiirtojärjestelmien ohjelmistopohjaisuus nostaa kyberuhkan merkitystä arvioitaessa eri uhkien kokonaismerkityksiä. Onnistunut kyberoperaatio voi tarkoittaa koko järjestelmän lamautumista tai vakavaa tietovuotoa. Merkittävimpänä esteenä kognitiivisen radion kehittymiselle on taajuusregulaation jäykkyys. Paine taajuushallinnan muutokselle nousee erityisesti siviilipuolen kehittyvien mobiililaitteiden datansiirtomäärien kasvaessa. Lainsäädäntö antaa viranomaisille suuret valtuudet säädellä spektrin käyttöä poikkeusoloissa. Tämä mahdollistaa sotilaallisten järjestelmien suunnittelun ja kehittämisen taajuusriippumattomasti ja toisaalta myös edellyttää sitä.

Social Networking Sites as a part of Medical Device Manufacturer's Marketing Communications

The purpose of the thesis is to examine how a medical device manufacturer can exploit social networking sites as a part of its everyday marketing communications. The ultimate goal is to create an ideal process of developing marketing communications in social networking sites as a medical device manufacturer with the help of theoretical knowledge and hands-on experience. Theoretical part examines the traditional process of developing marketing communications, defines social networking sites and presents marketing activities carried out on these sites as well as introduces the characteristics of healthcare technology industry. Empirical part is collected through participation in medical device manufacturer’s marketing operations and by observing effects of different factors and actions on social media marketing. In addition, completed interviews and a meeting with company’s personnel have been utilized for data collection. This part offers comprehensive information on the examined company’s current marketing operations, industry, and activities carried out on social networking sites. As a result of the thesis a comprehensive process description of integrating and using social networking sites as a part of company’s marketing communications was formed. With the help of the process description factors and actions which have an effect on marketing operations in social networking sites are presented and methods for further developing these activities are introduced.