Algunos aspectos de la reformulación del discurso científico

Fil: Móccero, María Leticia. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.

Impacto de la crisis socioeconómica de 2001 sobre los patrones de producción y difusión de los conocimientos del sistema científico argentino

En los últimos años varios trabajos han abordado el tema la crisis económica argentina de 2001, y sus principales repercusiones sobre el sistema político, social, institucional, e incluso el científico. Sin embargo, no hemos encontrado estudios que hayan analizado sus efectos desde una perspectiva cienciométrica. Este trabajo tiene por objeto mostrar el impacto de la crisis socioeconómica argentina de 2001 sobre el sistema científico, a partir de un conjunto de indicadores cienciométricos relativos al esfuerzo económico, los recursos humanos dedicados a la investigación, las publicaciones, las relaciones de colaboración y la visibilidad internacional de las contribuciones científicas

Las normativas del CONICET y la UNLP sobre la mención de la afiliación institucional en las publicaciones científicas. Problemas recurrentes y nuevas tensiones en el sistema científico y tecnológico argentino

Recientes normativas sobre el contenido y forma de mencionarla afiliación institucional de los investigadores de dos de las principales instituciones públicas de investigación de Argentina (la Universidad Nacional de La Plata y el Consejo Nacional de Investigaciones Científicas y Técnicas) obligan a reflexionar sobre problemas recurrentes y nuevas tensiones en torno a las relaciones institucionales del sistema científico-tecnológico argentino. Los objetivos de esta contribución son: 1) analizar las normativas de ambas instituciones; 2) señalar sus principales virtudes y falencias; y 3) alertar sobre los peligros de promulgar reglamentaciones no consensuadas. Las diferentes y hasta contradictorias normativas referidas a cómo los científicos deben registrar su afiliación institucional en las publicaciones están basadas en miradas aisladas, que colocan a numerosos investigadores con doble dependencia institucional en una encrucijada cuando deben cumplirlas. Los ejemplos analizados exponen la falta de coordinación inter-institucional y la necesidad de tener una mirada que contémplelas relaciones de cooperación nacional, regional e internacional en un escenario científico y tecnológico cada vez más globalizado. No parece razonable promulgar normativas que pongan a las instituciones estatales en una suerte de competencia por apropiarse de la producción científica de los investigadores, canibalizándose unas a otras. A contramano de la historia y del espíritu de colaboración que ha primado dentro del sistema argentino de ciencia y tecnología, estas visiones sesgadas ponen en serio riesgo el lugar privilegiado que ha logrado la Argentina entre los principales países productores de ciencia de América Latina, así como el reconocimiento internacional de sus instituciones científico-tecnológicas

Divulgação Científica na Revista Ciência Hoje das Crianças

Esta pesquisa trata da revista Ciência Hoje das Crianças, editada pela Sociedade Brasileira para o Progresso da Ciência (SBPC), e coloca em questão as especificidades da linguagem utilizada pela revista para a divulgação científica. A amostra utilizada é composta pelas edições publicadas no ano de 2008. O objetivo da pesquisa é identificar os recursos utilizados pela revista para adequação da linguagem científica para a linguagem coloquial, acessível ao público leigo. A metodologia utilizada para desenvolvimento da pesquisa tem viés qualitativo e adota os recursos da pesquisa bibliográfica, documental, entrevistas e análise de discurso da Escola Francesa. Constata-se que a revista busca contemplar com seus artigos todas as áreas do conhecimento, o que garante ao leitor a possibilidade de ampliar a visão sobre o mundo científico. Para construção da mensagem em linguagem acessível, identifica-se que a revista recorre à utilização de desenhos, fotos, cores, tipografia, metáforas, analogias e estrutura textual organizada, principalmente, em forma de narração.(AU)

TÁTICAS DE PROFESSORES: UMA REFLEXÃO SOBRE O CENÁRIO AVALIATIVO NO REGIME DE PROGRESSÃO CONTINUADA.

Este estudo qualitativo foi elaborado por meio de pesquisa bibliográfica de estudiosos do tema da avaliação do rendimento de alunos, como Luckesi (2005), Hoffmann (2003), Paro (2003), Hadji (1994) e Figari (1996), e de pesquisa de campo, com o objetivo de investigar como está ocorrendo a avaliação do ensino fundamental da rede pública paulista, na vigência do regime de progressão continuada. Considerando que os fundamentos da prática avaliativa distam quase sempre dos apregoados pela política educacional instituída e com o propósito de verificar se isto está ocorrendo com a política de progressão continuada, participei, durante um ano letivo, das atividades educacionais de uma escola pública da periferia da Grande São Paulo, observando, colhendo depoimentos e anotando cuidadosamente tudo o que vivenciei nesse período. Para empreender uma leitura dos dados coletados, busquei também o apoio no construto teórico de Michel de Certeau, para quem aos "produtos impostos" códigos, leis, políticas culturais, etc. rigorosamente organizados de forma a atribuir um lugar, um papel ao homem ordinário, contrapõem-se práticas construídas no processo de apropriação desses produtos. Por meio de suas "artes de fazer", os usuários reinventam o cotidiano. Utilizando-se de "táticas astuciosas", o usuário da cultura reapropria-se dos espaços, altera-lhes os códigos e deles faz uso "a seu jeito". Nesta pesquisa, que enfoca as relações dos protagonistas de processos avaliativos escolares, confirmam-se os postulados de Certeau, que não conferem ao consumidor da política um lugar passivo. Como já foi caracterizado por este instigante pensador, o usuário da política (o instituinte), enquanto portador de astúcias, move-se no campo espacial do outro (o instituído) e, taticamente, fazendo uso de práticas não previstas, escreve uma "outra história". O principal objetivo deste trabalho foi contribuir com reflexões que mostrem a importância de se analisar as tensões geradas por políticas educacionais impostas, desprovidas de sentido para aqueles que não participam de sua elaboração, o que tem provocado movimentos táticos de seus usuários, neste caso, os docentes, os quais, sem as condições objetivas necessárias para promover a política instituída, enunciada no discurso, utilizam-se do seu potencial instituinte de transformação, escrevendo uma "outra história".

Relatório de estágio realizado na Escola Secundária de Francisco Franco

O Estágio Pedagógico, realizado na Escola Secundária de Francisco Franco, constituiu um processo de formação, onde o professor estagiário teve a oportunidade de aplicar os seus conhecimentos, adquiridos no âmbito da licenciatura e do mestrado, no contexto escolar. Este relatório apresenta uma descrição e reflexão sobre o processo de Estágio, apresentando todos os procedimentos adotados e opções tomadas, relativamente às linhas programáticas do Estágio Pedagógico, isto é: a prática letiva, as atividades de intervenção na comunidade escolar, as atividades de integração no meio e as atividades de natureza científico-pedagógica. A prática letiva englobou a Gestão do processo de Ensino-Aprendizagem, onde adquiriram-se capacidades de planeamento, de realização e de controlo das aulas, procurando sempre promover um ambiente de aprendizagem favorável para a progressão dos alunos. Também contemplou as assistências às aulas de outros professores, onde foi possível observar e refletir relativamente a diferentes estratégias e metodologias de ensino. As atividades de intervenção na comunidade escolar abarcaram o projeto “O Franquinho”, que envolveu toda a comunidade escolar (professores, alunos, funcionários e encarregados de educação), e consistiu num evento pontual onde realizaram-se atividades relacionadas com a Educação Física e com as diferentes áreas do conhecimento, estabelecendo-se fenómenos de transdisciplinaridade. As atividades de integração no meio, relacionadas com a direção de turma, envolveram a caraterização da turma e um estudo de caso a um aluno com problemas cardíacos. Quanto à ação de extensão curricular realizou-se uma visita de estudo relacionada com a modalidade do Golfe. Na atividade de natureza científico-pedagógica individual explorou-se a importância do desenvolvimento da aptidão física nas aulas de Educação Física e na vida quotidiana. Na atividade de natureza científico-pedagógica coletiva abordaram-se temáticas relacionadas com a Educação Física e Desporto, nomeadamente as ideias e perspetivas do professor João Mateus e a sua aplicabilidade neste contexto.

Relatório de estágio de biologia e geologia realizado na Escola Básica e Secundária Gonçalves Zarco

Este Relatório, integrado no Mestrado em Ensino de Biologia e de Geologia no 3º Ciclo do Ensino Básico e no Secundário, descreve as atividades planeadas e desenvolvidas no âmbito do Estágio Pedagógico que decorreu no ano letivo de 2012/2013 na Escola Básica e Secundária Gonçalves Zarco. As atividades estão organizadas segundo o delineado para o Estágio Pedagógico, interligam-se entre si e evidenciam uma intervenção abrangente do professor estagiário enquanto futuro professor de Biologia e Geologia. Assim, no primeiro capítulo consta a contextualização das atividades pedagógicas desenvolvidas, uma breve descrição dos intervenientes neste Estágio e ainda a caracterização da Escola de acolhimento, com vista a conhecê-la não só do ponto de vista estrutural e organizacional, como também compreender as orientações educativas definidas pela Escola, de modo a rentabilizar os espaços físicos e os materiais didáticos proporcionados pela instituição. No capítulo seguinte, referente à prática letiva, é abordada toda a ação pedagógica, a gestão do processo de aprendizagem, bem como a reflexão do professor sobre a sua progressão e aquisição de competências ao longo do Estágio. São ainda incluídas as atividades de integração no meio, com o intuito de conhecer as turmas que lecionávamos, as quais incluem a caracterização de uma das turmas lecionadas (9º ano) e a realização de um estudo de caso. Por último, são relatadas outras atividades realizadas no decorrer deste Estágio Pedagógico que tiveram uma expressão individual e coletiva, nomeadamente a atividade de intervenção na comunidade escolar e a atividade de natureza científico-pedagógica. A realização das várias atividades pedagógicas apresentadas, as vivências experienciadas, a partilha e o trabalho conjuntos, foram importantes para nos consciencializar de todo o processo de construção e desenvolvimento da nossa identidade profissional e das motivações pessoais e, claro, do modo como estas estão relacionadas com a nossa ação no contexto real.

Estudo de um secador híbrido (solar-elétrico) para desidratação de frutas

The growing need for food is something that worries the world, which has a population that is growing at a geometric progression while their resources grows at an arithmetic progression. To alleviate this problem there are some proposals, including increased food production or reduce waste thereof. Many studies have been conducted in the world in order to reduce food waste that can reach 40% of production, depending on the region. For this purpose techniques are used to retard degradation of foods, including drying. This paper presents a design of a hybrid fruit dryer that uses solar energy and electric energy with automation of the process. To accomplish drying tests were chosen Typical fruits with good acceptability as processed fruits. During the experiments were measured temperature values at different points. Were also measured humidity values, solar radiation and mass. A data acquisition system was built using a Arduino for obtaining temperatures. The data were sent to a program named Secador de Frutas, done in this work, to plot the same. The volume of the drying chamber was 423 liters and despite the unusual size test using mirrors to increase the incidence of direct radiation, showed that the drier is competitive when compared with other solar dryers produced in Hydraulic Machines and Solar Energy Laboratory (LMHES ) UFRN. The drier has been built at a cost of 3 to 5 times smaller than industrial dryers that operate with the same load of fruit. And the energy cost to produce dried fruits was more feasible compared with such dryers that use LPG as an energy source. However, the drying time was longer.

Estudo da correlação das características clínico-patológicas do câncer colorretal com a expressão imunohistoquímica de proteínas da progressão tumoral

Except the non-melanoma skin tumors, colorectal cancer is the second most common in the Southeastern Region of Brazil, the third most common in the Southern and Central Regions. It is also the forth most common in the Northern Region and it is the fifth one in the Northeastern. To assess pathological and clinical variables of colorectal Cancer is crucial to know the possible conclusions for the survival of patients and point out the characteristics in the progress of tumor, such as the profile of tumor invasion and its angiogenesis. This work focuses on analyzing clinically and pathologically some settings in colorectal cancer patients (CRC) in the city of Natal and its countryside through those variables as parameters of prognosis and determine the level of protein expression, for instance: E-cadherin (E-cad), beta- -catenin (β-cat), galectin-3 (gal-3), matrix metalloproteinases (MMP) 2 and 9 and vascular-endothelial growth factor alpha (α VEGF) in the tumor tissues. A retrospective study was done in colorectal cancer cases in the regions of Rio Grande do Norte state from 1995 to 2005, specifically in Natal city/RN/Brazil. The pathological and clinical variables, such as: age, gender, ethnicity, lifestyle, family history, the location of the primary tumor, level of differentiation, TDM staging, modified Dukes’, treatment and survival were analyzed. The pathological and clinical data were collected from medical records through a specific form and were filed on Excel. A total of 534 patients were selected from the Pathology Department file in this institution, however, 176 patients were excluded. 358 patients were included for Epidemiological analysis and its clinical and pathological correlations were selected. 180 patients were also selected for histological and immunohistochemical studies. The tumor progression of these selected proteins mentioned before were analyzed. The Paraffin blocks of these samples were treated by Microarray Tissue technique and its blades subjected to immunohistochemistry to test the intensity of these proteins in tumor tissues. The results of this analysis were correlated with clinicopathologic variables of patients. Statistical analysis using the chi-frame Pearson test and analysis of midlife by Kaplan-Meier curve was also utilized. P values < 0.05 were considered statistically significant. The average age of our sample was 58.8 years and 51.7 % were female. Alcohol consumption has increased by 1.71 time the risk of death by CCR (p = 0.034) and tobacco consumption increased 2.7 times the chance of developing tumors of high TNM stage (p = 0.001). Cancer patients had a family history of 3,833 times the chance of developing the CCR (p = 0.002). The expression of MMP-2 showed a significant association with tumors of high TNM stage (p <0.046) and mortality (p = 0.041). The α VEGF expression had statistically significant correlation with high TNM stage (p <0.009), degree of cell indifferentiation (p <0.025) and mortality (p <0.035). Expressions of E-cadherin and beta-catetina demonstrated tumor linked to high TNM stage (p = 0.0001) and Dukes› modified (p = 0.05), lesions in the rectum (p = 0.03 and p = 0.007, respectively), smoking (p = 0.05) and indifferentiation (p = 0.001). The expression of Gal-3 showed statistical significance with high TNM stage of patients (p = 0.01), smokers (p = 0.01), alcohol drinking (p = 0.03), indifferentiation (p = 0.0001) and mortality (p = 0.0001). Based on the results, therefore, we could realize that lifestyle and family history had correlation in the CCR prognosis, as well as MMP-2 expression, MMP-9, VEGF alpha, E-cadherin, Beta-catenin and Galectin-3 were important prognostic markers in tumor progression in colorectal cancer.

Correlação da imunoexpressão do fator de choque térmico 1 (hsf1) com aspectos clinicopatológicos em carcinomas de células escamosas de língua oral

Squamous cell carcinoma of oral tongue shows high rates of morbidity and mortality in the population, therefore, great efforts are being made to classify morphological changes and identify biomarkers that have prognostic value and that are able to group patients in individualized therapeutic options. From this perspective, there is the heat shock factor 1 (HSF1), which is a heat shock factor transcription protein (HSPs) that allows the cancer to deal with stressors associated with malignancy, acting differently in tumor progression. This research aimed to perform a clinico-pathological analysis of 70 cases of oral tongue squamous cell carcinoma (OTSCC) and immunohistochemical study of the expression of HSF1 protein in OTSCC, comparing it with 30 specimens of normal oral mucosa (NOM), and correlating this immunostaining with clinico-pathological aspects of OTSCC. To analyze the association between immunoexpression of HSF1 and clinicophatoloical aspects, the cases were categorized in minor and major overexpression, based in the median immunostaining score. Regarding the cases of OTSCC, 57.1% showed clinical stage III or IV, 82.9% were graded as high grade according to Bryne (1998) and 47.1% as high risk of malignancy according to Brandwein-Gensler et al., (2005). A disease free survival rate of 47.84% and overall survival rate of 68.20% was observed in the analyzed cases, and the high degree of malignancy according to Bryne’s system (1998) (p=0.05), tumor size T3 or T4 (p=0.04), local recurrence (p=0.02), and perineural invasion (p=0.02) determined negative impacts in survival time. We observed also a statistically significant result (p<0.01) when comparing the immunoreactivity of HSF1 between NOM and OTSCC. This significantly increased expression of HSF1 in cases of OTSCC suggests that this protein acts, indeed, in the pathogenesis of this disease. However, there were no statistically significant associations between this overexpression and the clinico-pathological parameters analyzed. This finding may reflect the influence of epigenetic events on HSF1 gene or a possible stability of this protein expression throughout disease progression.

Correlação da imunoexpressão do fator de choque térmico 1 (hsf1) com aspectos clinicopatológicos em carcinomas de células escamosas de língua oral

Squamous cell carcinoma of oral tongue shows high rates of morbidity and mortality in the population, therefore, great efforts are being made to classify morphological changes and identify biomarkers that have prognostic value and that are able to group patients in individualized therapeutic options. From this perspective, there is the heat shock factor 1 (HSF1), which is a heat shock factor transcription protein (HSPs) that allows the cancer to deal with stressors associated with malignancy, acting differently in tumor progression. This research aimed to perform a clinico-pathological analysis of 70 cases of oral tongue squamous cell carcinoma (OTSCC) and immunohistochemical study of the expression of HSF1 protein in OTSCC, comparing it with 30 specimens of normal oral mucosa (NOM), and correlating this immunostaining with clinico-pathological aspects of OTSCC. To analyze the association between immunoexpression of HSF1 and clinicophatoloical aspects, the cases were categorized in minor and major overexpression, based in the median immunostaining score. Regarding the cases of OTSCC, 57.1% showed clinical stage III or IV, 82.9% were graded as high grade according to Bryne (1998) and 47.1% as high risk of malignancy according to Brandwein-Gensler et al., (2005). A disease free survival rate of 47.84% and overall survival rate of 68.20% was observed in the analyzed cases, and the high degree of malignancy according to Bryne’s system (1998) (p=0.05), tumor size T3 or T4 (p=0.04), local recurrence (p=0.02), and perineural invasion (p=0.02) determined negative impacts in survival time. We observed also a statistically significant result (p<0.01) when comparing the immunoreactivity of HSF1 between NOM and OTSCC. This significantly increased expression of HSF1 in cases of OTSCC suggests that this protein acts, indeed, in the pathogenesis of this disease. However, there were no statistically significant associations between this overexpression and the clinico-pathological parameters analyzed. This finding may reflect the influence of epigenetic events on HSF1 gene or a possible stability of this protein expression throughout disease progression.

Análise do perfil imunológico e citohistológico durante a transmissão vertical de Trypanosoma cruzi em diferentes estágios da infecção experimental

Trypanosma cruzi is the causative agent of Chagas disease. This trypanosomiasis has become a global public health problem due to migration of Latin Americans to non-endemic countries. In Latin America with the succesful implementation of control domiciliated vector infestation and blood transfusion, the importance of congenital transmission has recently increased. Considering the tight regulation of immune system during gestation, we aimed to investigate the changes in the immune system caused by T.cruzi infection in the gestation outcome. T cruzi G and Y strain were used to infect female BALB/c mice before or after mating with non-infected male mice. The presence of vaginal plug was used as indicative of mating. Females were euthanized 8 days after confirmation of vaginal plug. We used three female control groups, only infected, only infected and non-infected and non-pregnant females. Two groups were infected before mating and other two were infected 4 days after confirmation of vaginal plug. The uterus and spleen were collected to immunochemistry, qPCR, immunofluorescence and cytokine analysis. Our results showed that despite the MMP’s identification being similarly among groups, T.cruzi higher virulent strain can impaire gestation outcome prior mating; the infection also increased cytokines like IFN-γ, IL-1β and IL-4; and leucocytes in uterine environment was altered, responding locally to systemic changes caused by T.cruzi infection. In conclusion this work suggests that T.cruzi infection can impaire gestation outcome and local response to sistemic infection was able to control the infection allowing pregnancy development in some conditions.

Preparação e caracterização de biomateriais poliméricos para avaliação da viabilidade de uso como phantom biológico

The theoretical and experimental developments in the biomaterials area have been directly applied to different fields of Medicine (odontology, regenerative medicine and radiotherapy). These advances have focused both for diagnosing diseases such as for quantifying degrees of progression. From the perspective of these studies, biomaterials are being designed and manufactured for application in various areas of science, provided advances in diagnostic radiology, radiotherapy dosimetry and calibration of radiotherapy equipment. Develop a phantom from a biomaterial has become a great ally of medicine in the treat patients with oncological diseases, allowing better performance of the equipment in order to reduce damage to healthy tissue due to excessive exposure to radiation. This work used polymers: chitosan and gelatin, for making the polymeric structures and controlled for different types of production and processing, characterizing and evaluating the biopolymer by physical techniques (STL, SEM and DEI) and therefore analyze applicability as phantom mouse lung. It was possible to evaluate the morphology of biomaterials quantitatively by scanning electron microscopy associated with imaging technique. The relevance of this work focuses on developing a phantom from polymeric biomaterials that can act as phantom providing high image contrast when subjected to analysis. Thus, the choice of DEI technique is satisfactory since it is an imaging technique of X-ray high resolution. The images obtained by DEI have shown the details of the internal microstructure of the biomaterial produced which have ≈ 10 μm dimension. The phantoms had made density ranging from 0.08 a 0.13 g/cm3.

Atividade antiangiogênica da forma recombinante da proteína 21 de Trypanosoma cruzi

Chagas disease, caused by the parasite Trypanosoma cruzi, is the cause of Chronic chagasic cardiomyopathy (CCC). The prospection of innovative therapeutic agents against CCC is a major task. The recombinant form of 21 (rP21), a secreted T. cruzi protein involved in host cell invasion and on progression of chronic inflammatory processes have been studied as a potential novel therapeutic target. Our present work aimed to verify and investigate the impact of rP21 in the formation of blood vessels in vitro and in vivo. First, tEnd cells were treated with different concentrations of rP21 or bacterial extract and viability and cellular adhesion were evaluated by MTT and angiogenesis inhibition by Matrigel tube formation assay and murine model. To verify the proteolytic activity of rP21 on extracellular matrix (ECM) components, fibrinogen, matrigel and fibronectin was incubated with rP21 or not. In addition, we performed proliferation assays and cell cycle analysis. Furthermore, the accumulation and distribution of F-actin was determined by Phalloidin staining using ImageJ software. Finally, tEnd cells were incubated with rP21 and the mRNA levels were analyzed by real-time PCR. Our results showed that rP21 did not alter cell viability and adhesion, but strongly inhibited vessel formation in vitro and in vivo. Tube formation assay showed that angiogenesis inhibition was dependent of the CXCR4-rP21 binding. In addition to these results, we observed that the rP21 was able to inhibit cell proliferation and promoted a significant reduction in the number of 4n cells (G2/M phase). Moreover, we found that rP21 significantly increased F-actin levels and this protein was able to modulate expression of genes related to angiogenesis and actin cytoskeleton. However, rP21 showed no significant activity on the matrix components. In this sense, we conclude that the rP21-endothelial cells (ECs) interaction via CXCR4 promotes inhibition of vessel formation through a cascade of intracellular events, such as inhibition of ECs proliferation and modulation of the expression of molecules associated with angiogenic processes and actin cytoskeleton.