A preliminary study on the use of the local variety of ipil-ipil Leucaena leucocephala as a protein source for prawn feed

A study was carried out to determine the effect of 10 or 20% leaves or seeds in the diet of Penaeus monodon , and the extent to which local ipil-ipil (Leucaena leucocephala ) could replace head shrimp meal. A brief description is given of the experimental methodology, and details of composition of the diet, proximate chemical composition of the diets, mean weight gain and survival of Penaeus monodon larvae fed shrimp head meal and ipil-ipil as protein sources, are presented. Mean weight gains for all groups were poor and not statistically significant. Survival rates for those fed 10% ipil-ipil were significantly higher than those fed 20% diets. Wherever the survival rate was high, mean weight gain was low and vice versa. The presence of the toxic alkaloids mimosine in ipil-ipil could have caused the low survival rate.

Fishwater and agar as binders in a prawn diet

The objectives of the study were to find out the proportion of water to fish fins, skin and bones that would give a good gel and to determine the effect of a combination of fish water and commercial crude agar on the water stability of the prawn diet. Under the conditions of the experiments it was concluded: (1) Fish water and commercial agar or agar bar gave the most stable pellet, 65% water stability; (2) a strong gel is obtained when one part shark fin is boiled in 1.5 parts water; (3) more fish water can be obtained from guitar fish than from shark fish.

Ipil-ipil (Leucaena leucocephala) leaves as a plant protein source in prawn diets

Penaeus monodon juveniles were fed diets containing fish meal, shrimp head meal and ipil-ipil leaves soaked and unsoaked, local and peruvian varieties. Mean weight gain at the end of 8 wk was significantly highest among those given the diet containing commercial ipil-ipil leaves. Gain in length followed the same pattern as mean weight gains. Among the diets containing ipil-ipil leaves there was a direct relationship in the amount of mimosine in the diet and the survival rate, the lower the amount of mimosine (due to soaking) the higher the survival rate. The Results thus indicate the beneficial effect of the addition of commercial ipil-ipil leaves to the diets of prawns, providing the mimosine content is kept low by soaking. A reduce in costs is also obtained, since 1kg of shrimp head meal or fish costs more than 2 or 4 tons, respectively, than that of ipil-ipil foliage.

Evaluation of milkfish (Chanos chanos Forskal) and prawn (Penaeus monodon Fabricius) in polyculture systems

The growth, survival and profitability of C. chanos and P. monodon grown in 5 different combinations for 100 days in 500m2 brackish water ponds were assessed. Differences in the growth and production of prawns cultured singly or in combination with milkfish at increasing stocking density strongly suggests that the presence of milkfish exerts some negative effect on prawn. However, growth production and competition index data suggest that the presence of prawn do not significantly affect milkfish. While the maximum production of prawn can be attained in monoculture, its polyculture with 2000 milkfish/ha is also economically feasible.

Polyculture of milkfish (Chanos chanos) and tiger prawn (Penaeus monodon Fab.) with and without supplemental feeding

Twelve 1,000 m super(2) earthen ponds were used to compare the growth rates, survival and production of milkfish (Chanos chanos)) and prawn (Penaeus monodon ) in monoculture and polyculture systems in shallow brackishwater ponds and without supplemental feeding. The low production and survival rates obtained were attributed to the lack of natural food; the high salinity during the first month of culture could be one of the causes of the high mortality of prawn observed in both mono and polyculture systems. Although the results of the trial were not ecouraging, it is possible that high yields may be obtained from the combination of the 2 species given enough natural food and favourable water conditions.

Gum arabic, carrageenan of various types and sago palm starch as binders in prawn diets

An experiment was undertaken in order to investigate the use of sago palm starch, gum arabic and carrageenans as binders in prawn diets. Water stability data are presented; EPT-2 carrageenan was found to be the best binder for both steamed and unsteamed pellets.

Notes on the construction of 12 cu m ferrocement maturation tank for prawn broodstock

The study aimed to develop an economical, rational and easy method of constructing ferrocement tank suitable for P. monodon maturation.

Effect of chitin and prawn shell on the growth of albino rat

The effect of addition of pure chitin from prawn shell, deproteinised prawn shell, demineralized prawn shell and dry prawn shell in casein based control diet on albino rats was studied. The diets contained 0.5% chitin and 10% protein. The results obtained in the studies show that the weight gain and feed conversion were maximum in the control diet. While addition of pure chitin slightly brought down the weight gain, addition of deproteinsed prawn shell have the minimum weight gain showing that presence of minerals adversely affects both feed consumption and weight gain in the case of albino rats. Although it was reported that addition of pure chitin at 0.5% in the commercial feed of broiler chicken gave increased weight, in the case of albino rats the weight gain was slightly reduced compared to control diet.

Sequencing, annotation and comparative analysis of nine BACs of giant panda (Ailuropoda melanoleuca)

A 10-fold BAC library for giant panda was constructed and nine BACs were selected to generate finish sequences. These BACs could be used as a validation resource for the de novo assembly accuracy of the whole genome shotgun sequencing reads of giant panda newly generated by the Illumina GA sequencing technology. Complete sanger sequencing, assembly, annotation and comparative analysis were carried out on the selected BACs of a joint length 878 kb. Homologue search and de novo prediction methods were used to annotate genes and repeats. Twelve protein coding genes were predicted, seven of which could be functionally annotated. The seven genes have an average gene size of about 41 kb, an average coding size of about 1.2 kb and an average exon number of 6 per gene. Besides, seven tRNA genes were found. About 27 percent of the BAC sequence is composed of repeats. A phylogenetic tree was constructed using neighbor-join algorithm across five species, including giant panda, human, dog, cat and mouse, which reconfirms dog as the most related species to giant panda. Our results provide detailed sequence and structure information for new genes and repeats of giant panda, which will be helpful for further studies on the giant panda.

The sequence and de novo assembly of the giant panda genome

Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes.

Effects of control of C/N ratio by low-cost carbohydrate addition on water quality and pond ecology in freshwater prawn Macrobrachium rosenbergii post-larvae nursing system

An experiment was conducted to evaluate the effects of control of carbon/nitrogen ratio (C/N ratio) by addition of low cost carbohydrate to the water column on water quality and pond ecology in freshwater prawn Macrobrachium rosenbergii post-larvae nursing system. In this experiment, two level of dietary protein 20% and 35% without carbohydrate addition (‘P20' and ‘P35') and with carbohydrate addition (‘P20+CH' and ‘P35+CH') were compared in small ponds of 40 m² area stocked with 20 post-larvae (0.021 ± 0.001g) per m² . Maize flour was used as low cost carbohydrate and applied to the water column followed by the first feeding during the day. The addition of carbohydrate significantly reduced (p< 0.05) ammonia-nitrogen (NH sub(3)-N) and nitrite-nitrogen (NO sub(2) - N) of water in P20 + CH and P35 + CH treatments. It significantly increased (p< 0.05) the total heterotrophic bacteria (THB) population both in water and sediment. Fifty nine genera of plankton were identified belonging to the Bacillariophyceae (11), Chlorophyceae (21), Cyanophyceae (7), Dinophyceae (1), Rotifera (7) and Crustacea (9) without any significant difference (p>0.05) of total phytoplankton and zooplankton among the treatments. Survival rate of prawn was significantly lowest (p<0.05) in P20 and no significant difference (p>0.05) was observed between P20+CH and P35 treatments. Control of C/N ratio by the addition of low-cost carbohydrate to the pond water column benefited the freshwater prawn nursing practices in three ways (1) increased heterotrophic bacterial growth supplying bacterial protein augment the prawn post-larvae growth performances, (2) reduced demand for supplemental feed protein and subsequent reduction in feed cost and (3) reduced toxic NH sub(3)-N and NO sub(2)-N levels in pond nursing system.

Year-round and rotational freshwater prawn, Macrobrachium rosenbergii and paddy farming: soil quality, production and economics

A study was carried out at five upazillas (Dumuria, Fakirhat, Pirojpur sadar, Gopalgonj sadar and Kalia) of five southern districts of Khulna, Bagerhat, Pirojpur, Gopalgonj and Narail to understand the comparative production performance and any effect on soil quality in case of year-round golda (Macrobrachium rosenbergii), alternate prawn-paddy and year-round paddy farming. A direct interview of 55 farmers was taken, using pretested questionnaire, and soil samples were taken from selected farms before and after each crop. Among the five upazillas, farmers in Gopalgonj sadar and Kalia are not practicing year-round golda. Rotational golda-paddy farming has been recorded to result in maximum profit, with the highest of Tk. 310,912/ha/year in 2003 at Dumuria. Only paddy farming is less profitable than other two cropping patterns, irrespective of study sites. The nutrient status of soil in rotational golda and paddy farming has been found improved, compared to that of only golda or paddy farming.

Effects of fish and prawn culture on physico-chemical parameters of water and rice yield in rice fields

An experiment was conducted with five treatments i.e. rice combined with fish having regular urea fertilization (T1), rice combined with prawn having regular urea fertilization (T2), rice combined with fish with supplementary feeding (T3), rice combined with prawn with supplementary feeding (T4) and without fish and prawn (T5) was kept as control. The dissolved oxygen values obtained in treatments with fish both in morning and afternoon were lower than the values of prawn containing treatments and control. The values of nitrate-N, ammonia-N, phosphate-P and chlorophyll-a were higher in fish containing treatments than the prawn containing treatments and control. Between the two fish containing treatments the higher gross (539.44 kg/ha) and net (440.14 kg/ha) yield were obtained in T3 with supplementary feeding and the lower gross (424.88 kg/ha) and net (314.32 kg/ha) yield were recorded in T1 without supplementary feeding. Again, between two prawn containing treatments the higher gross (108.69 kg/ha) and net (81.92 kg/ha) yield were obtained in T4 with supplementary feeding and lower gross (64.32 kg/ha) and net (30.98 kg/ha) yield were recorded in T2 without supplementary feeding. The highest yield of rice grain (3.45 mt/ha) and straw (6.37 mt/ha) were obtained in T1 with fish having urea fertilization without feeding.

Benthic fauna and water quality in Gorganrood River, Iran

This present work study on water quality and benthic Macro invertebrate in Gorganrood, river. The research was carried out at 6 sampling site and the abundance and diversity of benthos were monitored along the length of river between 2007_2008_14 families were recorded in phyla, namely Annelidae, Mollusca,.... The greatest number of species was at 1 st station and the least number was at 6th station. The upper section supported more diverse community then the lower section. A low macro invertebrate abundance was observed during spring as a result of heavy rainfall and flood, and generally in all lowest section because of high valve of nitrogen and other nutrients. Water physiochemical parameters such as Phosphate, Nitrate,TPS and others were measured and water quality were studied through different indices such as saprobic system, Helsinhoff (FBI), BMWP and the results were compared and evaluated by physical chemical and parameters. The result indicated that the water quality in the up stream and the middle were good to fair, but the down stream qualities were pour at all sites.

The study of ecological condition on productivity of Tajan River estuary in Mazandaran Province Caspian Sea

This project was done during a one-year period (2006-2007) with the aim of assessing and evaluating the susceptible and vulnerable habitat of Tajan River estuarine region as well as identifying its ecological features. This region consists of Tajan estuarine region as one of the sub-basins of the Caspian Sea basin which covers a surface of 2km2. In this assessment, 6 riverine estuarine and marine stations were chosen in which non-biotic parameters such as temperature, salinity, dissolved oxygen, pH and nutrients, and biotic parameters such as variation, density, plankton, primary production by chlorophyll-a. Benthos variation density, silt and the organic materials of the sediments were sampled and measured monthly. The amount of chlorophyll-a concentration and primary production showed a lot of seasonal changes at these stations which ranged from0.3 to 96 mg/m3. The results from the primary productions indicated that the eastern station of the estuary had high concentrations of chlorophyll-a during all seasons (96mg/m3). The most important and dominant planktonic groups in this region included Bacillariophyta from plankton and copepoda from zooplankton. The most important Benthos communities consisted of Driessena polymorpha.Cerastoderma lamarki in estuarine region,Chironomus plumosus in riverine region and Hypaniola sp. In marine region. Assessing the annual variation in these three riverine, estuarine and marine regions, phytoplankton with 3.1, Zooplankton with 2.7 and Benthos with 1.9 Showed the most density in the estuarine region. Assessing the annual density, phytoplanktonic (6118967 no . in m3) and zooplanktonic (7272 no . in m3) communities showed the most density in the marine region. Assessing the statistical tests showed that the estuarine and riverine regions had a significant difference in planktonic density (p<0.005) compared with the marine region. Moreover, The zeoplanktonic density in the marine region had a significant difference (p<0.005) with estuarine and riverine regions. Tooki test and one-way variance Analysis showed that in assessing the planktonic groups (p<0.005) and Benthos (p<0.005), there was a significant difference in variation index between river with estuary, and estuary with the sea. The amount of the total annual live biomass of the Benthos resource in Tajan river estuarine region was estimated 757.66 g/m2.