Characterization of monocarboxylate transporters (MCTs) expression in soft tissue sarcomas: distinct prognostic impact of MCT1 sub-cellular localization

Background: Soft tissue sarcomas (STSs) are a group of neoplasms, which, despite current therapeutic advances, still confer a poor outcome to half of the patients. As other solid tumors, STSs exhibit high glucose consumption rates, associated with worse prognosis and therapeutic response. As highly glycolytic tumors, we hypothesized that sarcomas should present an increased expression of lactate transporters (MCTs).Methods: Immunohistochemical expression of MCT1, MCT2, MCT4 and CD147 was assessed in a series of 86 STSs and the expression profiles were associated with patients' clinical-pathological parameters.Results: MCT1, MCT4 and CD147 were mainly observed in the plasma membrane of cancer cells (around 60% for MCTs and 40% for CD147), while MCT2 was conspicuously found in the cytoplasm (94.2%). Importantly, we observed MCT1 nuclear expression (32.6%). MCT1 and MCT4, alone or co-expressed with CD147 in the plasma membrane, were associated with poor prognostic variables including high tumor grade, disease progression and shorter overall survival. Conversely, we found MCT1 nuclear expression to be associated with low grade tumors and longer overall survival.Conclusions: The present work represents the first report of MCTs characterization in STSs. We showed the original finding of MCT1 expression in the nucleus. Importantly, opposite biological roles should be behind the dual sub-cellular localization of MCT1, as plasma membrane expression of MCT1 is associated with worse patients' prognosis, while nuclear expression is associated with better prognosis.

Cellular and Molecular Tissue Response to Triple Antibiotic Intracanal Dressing

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cellular behavior as a dynamic field for exploring bone bioengineering: A closer look at cell-biomaterial interface

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

DNA Damage and Its Cellular Response in Mother and Fetus Exposed to Hyperglycemic Environment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Spectrum Distribution in Cognitive Radio: Error Correcting Codes Perspective

Cognitive radio is a growing zone in wireless communication which offers an opening in complete utilization of incompetently used frequency spectrum: deprived of crafting interference for the primary (authorized) user, the secondary user is indorsed to use the frequency band. Though, scheming a model with the least interference produced by the secondary user for primary user is a perplexing job. In this study we proposed a transmission model based on error correcting codes dealing with a countable number of pairs of primary and secondary users. However, we obtain an effective utilization of spectrum by the transmission of the pairs of primary and secondary users' data through the linear codes with different given lengths. Due to the techniques of error correcting codes we developed a number of schemes regarding an appropriate bandwidth distribution in cognitive radio.

A new transmission model in cognitive radio based on cyclic generalized polynomial codes for bandwidth reduction

The frequency spectrums are inefficiently utilized and cognitive radio has been proposed for full utilization of these spectrums. The central idea of cognitive radio is to allow the secondary user to use the spectrum concurrently with the primary user with the compulsion of minimum interference. However, designing a model with minimum interference is a challenging task. In this paper, a transmission model based on cyclic generalized polynomial codes discussed in [2] and [15], is proposed for the improvement in utilization of spectrum. The proposed model assures a non interference data transmission of the primary and secondary users. Furthermore, analytical results are presented to show that the proposed model utilizes spectrum more efficiently as compared to traditional models.

Key participants of the tumor microenvironment of the prostate: An approach of the structural dynamic of cellular elements and extracellular matrix components during epithelial-stromal transition

Cancer is a multistep process that begins with the transformation of normal epithelial cells and continues with tumor growth, stromal invasion and metastasis. The remodeling of the peritumoral environment is decisive for the onset of tumor invasiveness. This event is dependent on epithelial–stromal interactions, degradation of extracellular matrix components and reorganization of fibrillar components. Our research group has studied in a new proposed rodent model the participation of cellular and molecular components in the prostate microenvironment that contributes to cancer progression. Our group adopted the gerbil Meriones unguiculatus as an alternative experimental model for prostate cancer study. This model has presented significant responses to hormonal treatments and to development of spontaneous and induced neoplasias. The data obtained indicate reorganization of type I collagen fibers and reticular fibers, synthesis of new components such as tenascin and proteoglycans, degradation of basement membrane components and elastic fibers and increased expression of metalloproteinases. Fibroblasts that border the region, apparently participate in the stromal reaction. The roles of each of these events, as well as some signaling molecules, participants of neoplastic progression and factors that promote genetic reprogramming during epithelial–stromal transition are also discussed.

New ligands of the cellular retinoic acid-binding protein 2 (CRABP2) suggest a role for this protein in chromatin remodeling

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Duas poéticas da leitura: radição e invenção de precursores nos projetos literários de Jorge Luis Borges e Haroldo de Campos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Neonatal anoxia in rats: hippocampal cellular and subcellular changes related to cell death and spatial memory

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Leaf colleters in Tabernaemontana catharinensis (Apocynaceae, Rauvolfioideae): structure, ontogenesis, and cellular secretion

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Epigenetic and oncogenic regulation of SLC16A7 (MCT2) results in protein over-expression, impacting on signalling and cellular phenotypes in prostate cancer

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)