Characterization of monocarboxylate transporters (MCTs) expression in soft tissue sarcomas: distinct prognostic impact of MCT1 sub-cellular localization


Autoria(s): Pinheiro, Celine; Penna, Valter; Morais-Santos, Filipa; Abrahao-Machado, Lucas F.; Ribeiro, Guilherme; Curcelli, Emilio Carlos; Olivieri, Marcus V.; Morini, Sandra; Valenca, Isabel; Ribeiro, Daniela; Schmitt, Fernando C.; Reis, Rui M.; Baltazar, Fatima
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

03/12/2014

03/12/2014

09/05/2014

Resumo

Background: Soft tissue sarcomas (STSs) are a group of neoplasms, which, despite current therapeutic advances, still confer a poor outcome to half of the patients. As other solid tumors, STSs exhibit high glucose consumption rates, associated with worse prognosis and therapeutic response. As highly glycolytic tumors, we hypothesized that sarcomas should present an increased expression of lactate transporters (MCTs).Methods: Immunohistochemical expression of MCT1, MCT2, MCT4 and CD147 was assessed in a series of 86 STSs and the expression profiles were associated with patients' clinical-pathological parameters.Results: MCT1, MCT4 and CD147 were mainly observed in the plasma membrane of cancer cells (around 60% for MCTs and 40% for CD147), while MCT2 was conspicuously found in the cytoplasm (94.2%). Importantly, we observed MCT1 nuclear expression (32.6%). MCT1 and MCT4, alone or co-expressed with CD147 in the plasma membrane, were associated with poor prognostic variables including high tumor grade, disease progression and shorter overall survival. Conversely, we found MCT1 nuclear expression to be associated with low grade tumors and longer overall survival.Conclusions: The present work represents the first report of MCTs characterization in STSs. We showed the original finding of MCT1 expression in the nucleus. Importantly, opposite biological roles should be behind the dual sub-cellular localization of MCT1, as plasma membrane expression of MCT1 is associated with worse patients' prognosis, while nuclear expression is associated with better prognosis.

Formato

11

Identificador

http://dx.doi.org/10.1186/1479-5876-12-118

Journal Of Translational Medicine. London: Biomed Central Ltd, v. 12, 11 p., 2014.

1479-5876

http://hdl.handle.net/11449/111609

10.1186/1479-5876-12-118

WOS:000336931400001

WOS000336931400001.pdf

Idioma(s)

eng

Publicador

Biomed Central Ltd.

Relação

Journal of Translational Medicine

Direitos

openAccess

Palavras-Chave #Monocarboxylate transporters #CD147/EMMPRIN #Soft tissue sarcoma #Prognosis
Tipo

info:eu-repo/semantics/article