Acondicionamiento y mejora de la N-III, carretera convencional. Tramo desde pk. 35+000 hasta pk. 41+008, enlace con la A-3. Perales de Tajuña (Madrid)

En este Proyecto de Construcción se trata el acondicionamiento y mejora de un tramo de la N-III, entre el p.k. 35+000 y su enlace con la A-3, situado en la localidad madrileña de Perales de Tajuña. Esta carretera, que en otra época fue la principal vía de comunicación entre la capital de España y la costa levantina, ha visto reducida su importancia hasta convertirse en la práctica en una carretera de orden local. Se han estudiado, por tanto, distintas alternativas para adaptar las características de la vía a la demanda que actualmente presenta, prestando atención a todos los condicionantes que presenta, y se ha desarrollado la solución que se ha considerado más adecuada

Moderately High Temperatures Inhibit Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (Rubisco) Activase-Mediated Activation of Rubisco1

We tested the hypothesis that light activation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is inhibited by moderately elevated temperature through an effect on Rubisco activase. When cotton (Gossypium hirsutum L.) or wheat (Triticum aestivum L.) leaf tissue was exposed to increasing temperatures in the light, activation of Rubisco was inhibited above 35 and 30°C, respectively, and the relative inhibition was greater for wheat than for cotton. The temperature-induced inhibition of Rubisco activation was fully reversible at temperatures below 40°C. In contrast to activation state, total Rubisco activity was not affected by temperatures as high as 45°C. Nonphotochemical fluorescence quenching increased at temperatures that inhibited Rubisco activation, consistent with inhibition of Calvin cycle activity. Initial and maximal chlorophyll fluorescence were not significantly altered until temperatures exceeded 40°C. Thus, electron transport, as measured by Chl fluorescence, appeared to be more stable to moderately elevated temperatures than Rubisco activation. Western-blot analysis revealed the formation of high-molecular-weight aggregates of activase at temperatures above 40°C for both wheat and cotton when inhibition of Rubisco activation was irreversible. Physical perturbation of other soluble stromal enzymes, including Rubisco, phosphoribulokinase, and glutamine synthetase, was not detected at the elevated temperatures. Our evidence indicates that moderately elevated temperatures inhibit light activation of Rubisco via a direct effect on Rubisco activase.