Diagnóstico socioambiental da bacia do Ribeirão Água-Fria, Município de Bofete - SP

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Análise da presença de vírus em alho semente da segunda e quarta gerações, produzidos por termoterapia e cultura de tecido

The garlic (Allium sativum L.) can be naturally infected by a complex of filamentous viruses belonging to the genera Potyvirus, Carlavirus and Allexivirus. Accumulation of these viruses occurs especially by vegetative propagation through cloves. As the cultivated garlic plant does not produce true seed worldwide, virus-free plants can only be obtained by tissue culture of stem apices and thermotherapy. Using these techniques, garlic seeds were produced at the School of Agricultural Sciences - UNESP, Botucatu, and evaluated by RT-PCR for the presence of potyvirus, carlavirus and allexivirus. In the second generation of microcloves propagated in a greenhouse, 6.6% infection was detected, only by allexivirus. In the fourth generation, however, there was 60% incidence by allexivirus, 35% by potyvirus and all negative by carlavirus. The high rate of infection by allexivirus may be related to the greater difficulty of removing the species of viruses belonging to this genus, as observed by other authors, and also based on the infection and transmission of the virus by the mite, Aceria tulipae, during the storage of bulbs from one year to the other. The garlic at the fourth generation corresponds to cloves weighed less than 1 gram and not selected for commercial multiplication. Selection for the size of cloves has a positive effect on the choice of cloves with lower rates of viral infection, as the technique of thermotherapy and tissue culture do not eliminate the virus completely. Results also emphasize the need of fumigation for the garlic seed stored from one year to the other in order to prevent the transmission of allexivirus during storage.

Assessment of chemokine serum levels in epithelial ovarian cancer patients

Aims and background. The study was undertaken to investigate CCL2/MCP-1, CCL3/MIP-l alpha, CCL4/MIP-1 beta, CCL5/RANTES and CXCL8/IL-8 women with epithelial ovarian cancer.Methods and study design. Sixteen patients diagnosed with epithelial ovarian cancer and 18 healthy women with no evidence of malign neoplasia (control group) aged from 23 to 89 years (mean +/- SEM, 58.7 +/- 2.3) were included. The epithelial ovarian cancer patients underwent laparotomy and debulking surgery Chemokines serum levels were measured by cytometric bead array. Statistical analysis was performed using Mann-Whitney and Kendall's tau. P <0.05 was considered statistically significant for all analyses.Results. The tumor staging (FIGO) was classified into: I in 4 cases (25%), III in 5 cases (31.3%) and stage IV in 7 cases (43.8%). Sera chemokine dosages of CCL2 /MCP-1 and CCL4/MIP-1 beta were lower in epithelial ovarian cancer patients than in the control group (P = 0.021 and P = 0.030, respectively). No significant difference between groups was observed in the levels of CCL3/MIP-l alpha, CCL5/RANTES and CXCL8/IL-8. No association between the chemokines analyzed and tumor stage was found. The serum level of CCL4/MIP-1 beta was correlated with CA-125.Conclusions. The study of serum levels of CCL2/MCP-1, CCL3/MIP-l alpha, CCL4/MIP-1 beta, CCL5/RANTES and CXCL8/IL-8 chemokines in epithelial ovarian cancer patients identified a down-regulation in CCL2/MCP-1 and CCL4/MIP-1 beta, which suggests that the two chemokines may play an important role in the pathophysiology of ovarian cancer.

Determinação do nível de controle econômico de Enneothrips flavens Moulton, 1941 (Thysanoptera: Thripidae) em cultivar de amendoim de porte ereto

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Proliferating cell nuclear antigen immunoreaction in adrenal-tumors

Aims mid background: We studied, retrospectively, 33 cases of adrenal tumors of children at the Pediatric Endocrinology Unit, Children's Institute, Sao Paulo State University Medical School, from 1975 to 1993. Ail patients had at least 2 years of follow-up with a few exceptions. Methods: Clinical follow-up data were correlated with histopathologic review, laboratory data and cell kinetic evaluation (based on detection of proliferating cell nuclear antigens). Results: With one exception, all the patients had presented signs of androgen production and had high levels of dehydro-epiandrosterone-sulfate. Tumor weight evaluation represented a good parameter of neoplasm evolution: of 19 cases weighing less than 250 g, 17 had no evidence of disease after surgery, and 2 had an unfavorable prognosis. Of 14 cases weighing more than 250 g, only 1 had no evidence of disease and 13 had an unfavorable evolution. Conclusions: Proliferating cell nuclear antigen (PCNA) was not helpful to evaluate adrenal neoplasm evolution: our study did not show any correlation between PCNA score and prognosis.

Bulbilhos aéreos de alho, provenientes de escapes florais, são infectados por vírus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise morfométrica e simulação das áreas de preservação permanente de uma bacia hidrográfica

The morphometric characterization of watersheds is of great importance in assisting the planning of these areas to preserve the environment and maintain the quantity and quality of water production. The aim of this study was to characterize the morphology and simulate the areas of permanent preservation according to the Brazilian Forest Code of watershed of the Água-Fria stream. The studied area is located in the municipality of Bofete-SP, between the geographic coordinates: 48° 09' 30" to 48° 18' 30" longitude (WGr) and 22° 58' 30" to 23° 04' 30" latitude S. The results showed a 5th order micro watershed with an area of 152.43 km2 and low drainage density of 1.04 km/km2. Circularity was 0.51 and form factor was 0.41, which is considered low, and therefore with an oblong/oval shape. The sinuosity index of 1.29 revealed a tendency of rectilinear channels with compactness coefficient value of 1.38 and distance of runoff flow of 520m. Simulation of areas of permanent preservation shows an ideal model as the way springs and watercourses should be protected according to the Brazilian Forest Code, amounting to an area of 10.02 km2.

Caracterização do uso da terra em diferentes épocas na bacia hidrográfica do ribeirão paraíso- são manuel sp, por meio de sistemas de informações geográficas

This study aimed to evaluate a period of 38 years, the use and soil occupation of the Paradise River watershed, inserted in the citys of São Manuel and Areiópolis-SP using aerial photographs for the year 1972 and TM image (Thematic Mapper) obtained by the Landsat-5 satellite, in 2010, using geoprocessing techniques. The watershed in question is very important for the city of São Manuel-SP, because its urban area is inserted in its divisors which part of it belongs to the Environmental Protection Area (APA) Perimeter Botucatu-SP, considered a recharge area of the aquifer Guarani. Today, the development of agriculture faces challenges, which is to produce more food without impacting the environment. Allied to this concern, research institutions have sought new technologies that allow the detection and quantification of human actions, enabling interventions in order to minimize possible damage to the environment. Among these technologies can be cited Geographic Information Systems (GIS), which a large volume of data and information stored in a region at different times can be evaluated in the same time, suggesting different approaches to the planning of land use. The results of the mapping of areas of use and soil occupation result nine classes in 1972, and the coffee culture showed the biggest occupation (37.94%) of the total area. The 2010 mapping formulated twelve classes of use, which demonstrated the predominance of sugar cane (37.94%), on the areas occupied by coffee and pasture before. The land use maps of 1972 and 2010 showed results that show intense human activity in the modification of natural landscape.

SIG aplicado na caracterização morfométrica da microbacia do Ribeirão Morro Grande - Bofete, SP

Esse trabalho objetivou a aplicação de geoprocessamento na caracterização morfométrica da microbacia do Ribeirão Morro Grande – Bofete (SP) através do Sistema de Informação Geográfica – Selva, visando à preservação, racionalização do seu uso e recuperação ambiental. A microbacia apresenta uma área de 4049ha e está localizada entre os paralelos 22o 50' 05" a 22o 54' 26" de latitude S e 48o 22' 29" a 48o 26' 36" de longitude W Gr. A base cartográfica utilizada foi a carta planialtimétrica de Bofete (SP), em escala 1:50.000 (IBGE, 1968) na extração das curvas de nível, da hidrografia e da topografia, em ambiente de Sistema de Informações Geográficas - Idrisi Selva, para determinação dos índices morfométricos. Os resultados mostram que os baixos valores da densidade de drenagem, associados à presença de rochas permeáveis, facilitam a infiltração da água no solo, diminuindo o escoamento superficial e o risco de erosão e da degradação ambiental, bem como o baixo valor do fator de forma amparado pelo índice de circularidade indica que a microbacia tende a ser mais alongada com menor susceptibilidade à ocorrência de enchentes mais acentuadas. O parâmetro ambiental coeficiente de rugosidade permitiu classificar a microbacia para vocação com floresta e reflorestamento.

Avaliação das áreas de conflito de uso em APP da microbacia do alto água fria, Bofete (SP)

The misuse of land by man and is causing serious problems to the environment, resulting in ecological imbalance and depletion of natural resources, especially when it comes to areas of permanent preservation, that ecosystems are essential for balance and water conservation importance . Conflicts of land use is a major cause of silting of rivers and water bodies, causing significant losses in soil and decaying natural water resources both in quantity and in quality. Within this scenario, the planning becomes essential for the control of environmental impacts to the environment factor. This study aimed to evaluate land use conflicts in permanent preservation areas of the stream Água Fria watershed, Bofete (SP) using Geographic Information System and satellite image of LANDSAT - 5 of 2010, scale 1:50000. The results show that 51.16% of the permanent preservation areas of the watershed are conflicting, especially for pasture (88.94%) and reforestation with eucalyptus (11.06%). The GIS IDRISI Selva and Cartalinx along with GIS techniques demonstrated speed and efficiency in identifying, measuring and editing maps of land use, preservation and ongoing conflicts in areas of permanent preservation.

Adequabilidade das áreas de vegetação ripária da microbacia do Córrego Comur - Botucatu (SP) em função da legislação ambiental

The inadequate soil use is an aggravating factor of the environmental degradation and ecological unbalance. The analysis of the use and covering of the soil, by information of Remote Sensing, constitutes a technique of great usefulness to the planning and administration of the ordered occupation and rational of the physical middle, besides making possible to evaluate and to monitor the preservation of areas of natural vegetation. This work sought to evaluate the conflicts of soil use in permanent preservation areas (PPA) in Stream Comur watershed - Botucatu (SP) through Geographical Information System and satellite image of 2009. The study area is located among the geographical coordinates 48o 23’ 04” to 48o 25’ 54” of longitude WGr. and 22o 44’ 42” to 22o 48” 12” of latitude S with an area of 1,719.6 ha. The results allowed to verify that the geoprocessing techniques were of fundamental importance in the identification of the areas of soil use, of APP and of conflicts among use and PPA where it leaves of the areas of APP is being used inadequately. In terms of environmental sustainability, it can be deduced that the watershed is very unfavorable, once it presents 70.67% of area used inadequately with sugarcane and pasture.

Avaliação das áreas de conflito de uso em APP na microbacia do ribeirão Morro Grande

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Diagnóstico de potenciliade das unidades ambientais na bacia do Rio Capivara, Botucatu - SP

This work has the objective to elaborate the potentialities of the farming expand diagnosis of the Capivara River Hydrographic basin, in the Botucatu – SP municipal district, to obtain an interpretation of the system actual situation through the historical trajectory and possible evolution with the assistance of the SIG Ilwis 3.4 and orbital images CBERS 2B, in the pancromatics bands, obtained in 12/07/2008. The potentialities of the farming expand diagnosis was obtained through the physical mean modeling, of the farming expand presents in the studied environment and shows possible line actions to minimize the impacts e reach a sustainable development to the region. The Capivara river basin presents more than 70% of your areas occupied by the anthropic expanding, with the extensive farming predominance, forestall planting, orange and sugar cane. In the potentiality diagnostic analyses notes the good quality of the basin to the farming expand, except the region comprehended by the Botucatu Cuesta and the meadows area. The basin until has a good environmental quality and this diagnosis must serve for a start point to the realization of the territorial ordination as quick as possible to the environment conditions finds remains the same, granting the recovery capability.

BK virus associated meningoencephalitis in an AIDS patient treated with HAART

A severely immune-suppressed AIDS patient was suspected of suffering from BK virus (BKV) meningoencephalitis, after being studied for common causes of neurological complications of co-infectious origin. Polymerase chain reaction (PCR) and sequence analysis of cerebrospinal fluid and brain samples, confirmed the presence of BKV. His clinical condition improved along with the regression of brain lesions, after modifications on his antiretroviral regime. Five months after discharge, the patient was readmitted because of frequent headaches, and a marked inflammatory reaction was evidenced by a new magnetic resonance imaging (MRI). The symptoms paralleled a rising CD4+ lymphocyte count, and immune reconstitution syndrome was suspected. This is the first non-postmortem report of BKV meningoencephalitis in an AIDS patient, showing clinical and radiographic improvement solely under HAART.

Le neoplasie del gatto. Ricerche istologiche e immunoistochimiche

Il contributo di ricerca di questo lavoro è consistito nel rivalutare da un punto di vista diagnostico, classificativo e statistico, una casistica di neoplasie del gatto archiviate in 23 anni (1984-2006). Nei primi 20 anni sono state diagnosticate 1696 neoplasie su un totale di 3682 campioni prevenuti nello stesso periodo, con una prevalenza delle patologie di tipo neoplastico del 46,06%. Nei tre anni successivi sono state individuate 382 neoplasie su un totale di 601 campioni archiviati, con una prevalenza del 61,02%. Le neoplasie avevano per l'81,8% un comportamento maligno, e questo dato risulta coerente con il dato più recente sulla malignità (85,6%) e con quello fornito da altri autori. L'età dei soggetti affetti era simile nei due studi, compresa fra pochi mesi e 22 anni, con il maggior numero di casi fra 9 e 13 anni. Le due casistiche presentano inoltre dati simili riguardo gli organi coinvolti anche se i tumori della mammella sono notevolmente diminuiti negli ultimi anni, con un aumento corrispondente di neoplasie cutanee. I fibrosarcomi, che hanno subito un drastico aumento tra il 1993 e il 1994 si sono mantenuti costanti in tutti gli anni successivi, dato ancora oggi quasi certamente correlabile alle pratiche vaccinali. I linfomi sono risultati piuttosto infrequenti in entrambe le casistiche, comparate con i dati della letteratura in cui vengono riportate percentuali nettamente maggiori. I tumori del cavo orale e dell'intestino hanno mantenuto circa le stesse percentuali di insorgenza nel corso degli anni. Tra i tumori presenti in questo studio, sono stati approfonditi i tumori a carico delle sierose (mesoteliomi) e del polmone attraverso metodiche immunoistochimiche e ultrastrutturali. Nei 23 anni sono stati diagnosticati 10 casi di mesotelioma, di cui 8 coinvolgenti la pleura e due il peritoneo. Su questi casi è stata applicata una metodica immunoistochimica con un pannello anticorpale utilizzato di routine in patologia umana per la diagnosi di mesotelioma e per la diagnosi differenziale con i carcinomi polmonari. Tutti i casi presentavano una doppia positività per vimentina e citocheratine ad ampio spettro; anticorpi specifici come CK5/6 e HBME-1 hanno presentato una buona specificità. Anche la microscopia elettronica si è rivelata di ausilio, mettendo in evidenza la presenza di tonofilamenti citoplasmatici e di microvilli, tipici delle cellule mesoteliali. Le neoplasie polmonari, analogamente a quelle umane hanno, sono notevolmente aumentate negli ultimi anni. Dei 24 casi raccolti tra il materiale di archivio, 14 sono stati diagnosticati negli ultimi 5 anni. Si è rilevata una significativa predisposizione nei gatti di razza Persiana, e le metastasi più frequenti sono state a carico dei linfonodi, del miocardio e dei cuscinetti plantari, analogamente a quanto segnalato in letteratura. L’istotipo più rappresentato è stato il carcinoma squamoso, seguito da adenocarcinomi papillari e bronchioloalveolari. Il pannello immunoistochimico applicato, finalizzato a individuare l’origine broncogena o ghiandolare della neoplasia, è risultato utile per la diagnosi delle forme squamose poco differenziate (CK 5/6 e CK 14). Infine è stato testato l’anticorpo di elezione nella diagnosi differenziale fra le neoplasie primitive e secondarie del polmone, il TTF-1, che però non ha cross-reagito con tessuti di gatto; la diagnosi di neoplasia primitiva polmonare si è dunque basata sull’assenza, anche nei dati anamnestici, di masse in altri distretti corporei.