Indigestible cellulose and lignin in determining feces production and apparent digestibility in horses

Four crossbred geldings were used in a randomized blocks experimental design. The objective was to study the use of the internal markers indigestible cellulose (iCEL) and indigestible lignin (iLIG), obtained in situ (cattle) or in vivo (equine) to predict nutrient apparent digestibility in horses. Treatments consisted of different methodologies to determine digestibility: direct method with total feces collection (TC), and indirect method using internal markers iCEL and iLIG obtained either by in situ incubation in bovine rumen or in vivo (IV) using the mobile nylon bag (MNB) technique in horses. Feces production was 2.80 kg in DM, and average recovery rate (p > 0.05) was 101%. Nutrient digestibility coefficient (p > 0.05) estimates were adequately predicted by iCEL and iLIG, obtained in situ or in vivo, with average values of 52.63, 54.17, 64.90, 43.73 and 98.28% for dry matter, organic matter, crude protein, neutral detergent fiber and starch, respectively. It can be concluded that iCEL and iLIG may be obtained in vivo by MNB in horses consuming a forage-concentrate diet, to predict nutrient digestibility coefficients.

In vitro study on radiographic gray levels of biomaterials using two digital image methods

PURPOSE: To compare the direct and indirect radiographic methods for assessing the gray levels of biomaterials employing the Digora for Windows and the Adobe Photoshop CS2 systems. METHODS: Specimens of biomaterials were made following manusfacturer's instructions and placed on phosphor storage plates (PSP) and on radiographic film for subsequent gray level assessment using the direct and indirect radiographic method, respectively. The radiographic density of each biomaterial was analyzed using Adobe Photoshop CS2 and Digora for Windows software. RESULTS: The distribution of gray levels found using the direct and indirect methods suggests that higher exposure times are correlated to lower reproducibility rates between groups. CONCLUSION: The indirect method is a feasible alternative to the direct method in assessing the radiographic gray levels of biomaterials, insofar as significant reproducibility was observed between groups for the exposure times of 0.2 to 0.5 seconds.

L'inerbimento nell'ecosistema vigneto: confronto di metodologie di misura del consumo idrico del prato e risposta allo sfalcio

Water resources management will become increasingly important in agriculture as global warming takes place. Cover crop is largely used in viticultural areas based on the many positive agronomic and environmental benefits it provides. However, it is not clear what effect the cover crop can have on water use in the vineyard. This study is designed to develop a further understanding of the role cover crops play in total water use in the vineyard and develop our understanding of the potential use of cover crops as a water management tool. Two techniques were used to measure cover crop water use, the mini-lysimeters and a portable open chamber and data from both was compared to reference evapotranspiration (ETo) (FAO guidelines). While the mini-lysimeters seemed to be limited in their ability to accurately represent the water use of the surrounding soil, the open chamber method is a reliable and suitable instrument to be used for the accurate measurement of evapotranspiration. Further, the relationship between vineyard grass water use and the contributing environmental factors thought to influence water use were analyzed. A strong relationship between total available radiation and cover crop evapotranspiration was found suggesting the possibility of an indirect method of evapotranspiration measurement in a vineyard grass cover crop. Mowing the cover crop was determined to significantly effect transpiration as shown by both the mini-lysimeter and open chamber, however, the reduction was largely dependent on the growth rate of the grass.

Estimation of male-to-female ratios of mutation rate in primates and rodents

Haldane (1935) developed a method for estimating the male-to-female ratio of mutation rate ($\alpha$) by using sex-linked recessive genetic disease, but in six different studies using hemophilia A data the estimates of $\alpha$ varied from 1.2 to 29.3. Direct genomic sequencing is a better approach, but it is laborious and not readily applicable to non-human organisms. To study the sex ratios of mutation rate in various mammals, I used an indirect method proposed by Miyata et al. (1987). This method takes advantage of the fact that different chromosomes segregate differently between males and females, and uses the ratios of mutation rate in sequences on different chromosomes to estimate the male-to-female ratio of mutation rate. I sequenced the last intron of ZFX and ZFY genes in 6 species of primates and 2 species of rodents; I also sequenced the partial genomic sequence of the Ube1x and Ube1y genes of mice and rats. The purposes of my study in addition to estimation of $\alpha$'s in different mammalian species, are to test the hypothesis that most mutations are replication dependent and to examine the generation-time effect on $\alpha$. The $\alpha$ value estimated from the ZFX and ZFY introns of the six primate specise is ${\sim}$6. This estimate is the same as an earlier estimate using only 4 species of primates, but the 95% confidence interval has been reduced from (2, 84) to (2, 33). The estimate of $\alpha$ in the rodents obtained from Zfx and Zfy introns is ${\sim}$1.9, and that deriving from Ube1x and Ube1y introns is ${\sim}$2. Both estimates have a 95% confidence interval from 1 to 3. These two estimates are very close to each other, but are only one-third of that of the primates, suggesting a generation-time effect on $\alpha$. An $\alpha$ of 6 in primates and 2 in rodents are close to the estimates of the male-to-female ratio of the number of germ-cell divisions per generation in humans and mice, which are 6 and 2, respectively, assuming the generation time in humans is 20 years and that in mice is 5 months. These findings suggest that errors during germ-cell DNA replication are the primary source of mutation and that $\alpha$ decreases with decreasing length of generation time. ^

Noble gases sampled from three cruises in the eastern tropical Atlantic in September 2005 and June/July 2006

Upwelling velocities w in the equatorial band are too small to be directly observed. Here, we apply a recently proposed indirect method, using the observed helium isotope (3He or 4He) disequilibria in the mixed layer. The helium data were sampled from three cruises in the eastern tropical Atlantic in September 2005 and June/July 2006. A one-dimensional two-box model was applied, where the helium air-sea gas exchange is balanced by upwelling from 3He-rich water below the mixed layer and by vertical mixing. The mixing coefficients Kv were estimated from microstructure measurements, and on two of the cruises, Kv exceeded 1 x 10**-4 m**2/s, making the vertical mixing term of the same order of magnitude as the gas exchange and the upwelling term. In total, helium disequilibrium was observed on 54 stations. Of the calculated upwelling velocities, 48% were smaller than 1.0 x 10**-5 m/s, 19% were between 1.0 and 2.0 x 10**-5 m/s, 22% were between 2.0 and 4.0 x 10**-5 m/s, and on 11% of upwelling velocities exceeded this limit. The highest upwelling velocities were found in late June 2006. Meridional upwelling distribution indicated an equatorial asymmetry with higher vertical velocities between the equator and 1° to 2° south compared to north of the equator, particularly at 10°W. Associated heat flux into the mixed layer could be as high as 138 W/m**2, but this depends strongly on the chosen depths where the upwelled water comes from. By combining upwelling velocities with sea surface temperature and productivity distributions, a mean monthly equatorial upwelling rate of 19 Sv was estimated for June 2006 and a biweekly mean of 24 Sv was estimated for September 2005.

Experimental and numerical investigation of effect of sawn timber dimensions in ultrasonic velocity measurements of Spanish softwoods

Ultrasonic sound velocity measurements with hand-held equipment remain due to their simplicity among the most used methods for non-destructive grading of sawn woods, yet a dedicated normalization effort with respect to strength classes for Spanish species is still required. As part of an ongoing project with the aim of definition of standard testing methods, the effect of the dimensions of commonly tested Scots pine (Pinus sylvestris L.) timbers and equipment testing frequency on ultrasonic velocity were investigated. A dedicated full-wave finite-difference time-domain software allowed simulation of pulse propagation through timbers of representative length and section combinations. Sound velocity measurements vL were performed along the grain with the indirect method at 22 kHz and 45 kHz for grids of measurement points at specific distances. For sample sections larger than the cross-sectional wavelength ?RT, the simulated sound velocity vL converges to vL = (CL/?)0.5. For smaller square sections the sound velocity drops down to vL = (EL/?)0.5, where CL, EL and ? are the stiffness, E-modul and density, respectively. The experiments confirm a linear regression between time of flight and measurement distance even at less than two wavelength menor que2?L distance, the fitted sound speed values increased by 15% between the two tested frequencies.

Electrodynamic Tethers For Planetary And De-orbiting Missions

Nuevas aplicaciones tecnológicas y científicas mediante amarras electrodinámicas son analizadas para misiones planetarias. i) Primero, se considera un conjunto de amarras cilíndricas en paralelo (veleros electrosolares) para una misión interplanetaria. Los iones provenientes del viento solar son repelidos por el alto potencial de dichas amarras generando empuje sobre el velero. Para conocer el intercambio de momento que provocan los iones sobre las amarras se ha considerado un modelo de potencial estacionario. Se ha analizado la transferencia orbital de la Tierra a Júpiter siguiendo un método de optimización de trayectoria indirecto. ii) Una vez que el velero se encuentra cerca de Júpiter, se ha considerado el despliegue de una amarra para diferentes objetivos científicos. iia) Una amarra podría ser utilizada para diagnóstico de plasmas, al ser una fuente efectiva de ondas, y también como un generador de auroras artificiales. Una amarra conductora que orbite en la magnetosfera jovial es capaz de producir ondas. Se han analizado las diferentes ondas radiadas por un conductor por el que circula una corriente constante que sigue una órbita polar de alta excentricidad y bajo apoápside, como ocurre en la misión Juno de la NASA. iib) Además, se ha estudiado una misión tentativa que sigue una órbita ecuatorial (LJO) por debajo de los intensos cinturones de radiación. Ambas misiones requiren potencia eléctrica para los sistemas de comunicación e instrumentos científicos. Las amarras pueden generar potencia de manera más eficiente que otros sistemas que utlizan paneles solares o sistemas de potencia de radioisótopos (RPS). La impedancia de radiación es necesaria para determinar la corriente que circula por todo el circuito de la amarra. En un modelo de plasma frío, la radiación ocurre principalmente en los modos de Alfven y magnetosónica rápida, mostrando un elevado índice de refracción. Se ha estudiado la impedancia de radiación en amarras con recubrimiento aislante para los dos modos de radiación y cada una de las misiones. A diferencia del caso ionosférico terrestre, la baja densidad y el intenso campo magnético que aparecen en el entorno de Júpiter consiguen que la girofrecuencia de los electrones sea mucho mayor que la frecuencia del plasma; esto hace que el espectro de potencia para cada modo se modifique substancialmente, aumentando la velocidad de Alfven. Se ha estimado también la impedancia de radiación para amarras sin aislante conductor. En la misión LJO, un vehículo espacial bajando lentamente la altitud de su órbita permitiría estudiar la estructura del campo magnético y composición atmosférica para entender la formación, evolución, y estructura de Júpiter. Adicionalmente, si el contactor (cátodo) se apaga, se dice que la amarra flota eléctricamente, permitiendo emisión de haz de electrones que generan auroras. El continuo apagado y encendido produce pulsos de corriente dando lugar a emisiones de señales, que pueden ser utilizadas para diagnóstico del plasma jovial. En Órbita Baja Jovial, los iones que impactan contra una amarra polarizada negativamente producen electrones secundarios, que, viajando helicoidalmente sobre las líneas de campo magnético de Júpiter, son capaces de alcanzar su atmósfera más alta, y, de esta manera, generar auroras. Se han identificado cuáles son las regiones donde la amarra sería más eficiente para producir auroras. iic) Otra aplicación científica sugerida para la misión LJO es la detección de granos cargados que orbitan cerca de Júpiter. Los electrones de alta energía en este ambiente pueden ser modelados por una distribucción no Maxwelliana conocida como distribución kappa. En escenarios con plasmas complejos, donde los campos eléctricos en Júpiter pueden acelerar las cargas hasta velocidades que superen la velocidad térmica, este tipo de distribuciones son muy útiles. En este caso las colas de las distribuciones de electrones siguen una ley de potencias. Se han estudiado las fluctuaciones de granos cargados para funciones de distribución kappa. iii) La tesis concluye con el análisis para deorbitar satélites con amarras electrodinámicas que siguen una Órbita Baja Terrestre (LEO). Una amarra debe presentar una baja probabilidad de corte por pequeño debris y además debe ser suficientemente ligero para que el cociente entre la masa de la amarra y el satélite sea muy pequeño. En este trabajo se estiman las medidas de la longitud, anchura y espesor que debe tener una amarra para minimizar el producto de la probabilidad de corte por el cociente entre las masas de la amarra y el satélite. Se presentan resultados preliminares del diseño de una amarra con forma de cinta para deorbitar satélites relativamente ligeros como Cryosat y pesados como Envisat. Las misiones espaciales a planetas exteriores y en el ámbito terrestre plantean importantes retos científico-tecnológicos que deben ser abordados y solucionados. Por ello, desde el inicio de la era espacial se han diseñando novedosos métodos propulsivos, sistemas de guiado, navegación y control más robustos, y nuevos materiales para mejorar el rendimiento de los vehículos espaciales (SC). En un gran número de misiones interplanetarias y en todas las misiones a planetas exteriores se han empleado sistemas de radioisótopos (RPS) para generar potencia eléctrica en los vehículos espaciales y en los rovers de exploración. Estos sistemas emplean como fuente de energía el escaso y costoso plutonio-238. La NASA, por medio de un informe de la National Academy of Science (5 de Mayo del 2009), expresó una profunda preocupación por la baja cantidad de plutonio almacenado, insuficiente para desarrollar todas las misiones de exploración planetaria planeadas en el futuro [81, 91]. Esta circustancia ha llevado a dicha Agencia tomar la decisión de limitar el uso de estos sistemas RPS en algunas misiones de especial interés científico y una recomendación de alta prioridad para que el Congreso de los EEUU apruebe el reestablecimiento de la producción de plutonio-238, -son necesarios cerca de 5 kg de este material radiactivo al año-, para salvaguardar las misiones que requieran dichos sistemas de potencia a partir del año 2018. Por otro lado, la Agencia estadounidense ha estado considerando el uso de fuentes de energía alternativa; como la fisión nuclear a través del ambicioso proyecto Prometheus, para llevar a cabo una misión de exploración en el sistema jovial (JIMO). Finalmente, dicha misión fue desestimada por su elevado coste. Recientemente se han estado desarrollando sistemas que consigan energía a través de los recursos naturales que nos aporta el Sol, mediante paneles solares -poco eficientes para misiones a planetas alejados de la luz solar-. En este contexto, la misión JUNO del programa Nuevas Fronteras de la NASA, cuyo lanzamiento fue realizado con éxito en Agosto de 2011, va a ser la primera misión equipada con paneles solares que sobrevolará Júpiter en el 2015 siguiendo una órbita polar. Anteriormente se habían empleado los antes mencionados RPS para las misiones Pioneer 10,11, Voyager 1,2, Ulysses, Cassini-Huygens y Galileo (todas sobrevuelos excepto Galileo). Dicha misión seguirá una órbita elíptica de alta excentricidad con un periápside muy cercano a Júpiter, y apoápside lejano, evitando que los intensos cinturones de radiación puedan dañar los instrumentos de navegación y científicos. Un tether o amarra electrodinámica es capaz de operar como sistema propulsivo o generador de potencia, pero también puede ser considerado como solución científicotecnológica en misiones espaciales tanto en LEO (Órbita Baja Terrestre) como en planetas exteriores. Siguiendo una perspectiva histórica, durante las misiones terrestres TSS-1 (1992) y TSS-1R (1996) se emplearon amarras estandard con recubrimiento aislante en toda su longitud, aplicando como terminal anódico pasivo un colector esférico para captar electrones. En una geometría alternativa, propuesta por J. R. Sanmartín et al. (1993) [93], se consideró dejar la amarra sin recubrimiento aislante (“bare tether”), y sin colector anódico esférico, de forma que recogiera electrones a lo largo del segmento que resulta polarizado positivo, como si se tratara de una sonda de Langmuir de gran longitud. A diferencia de la amarra estandard, el “bare tether” es capaz de recoger electrones a lo largo de una superficie grande ya que este segmento es de varios kilómetros de longitud. Como el radio de la amarra es del orden de la longitud de Debye y pequeño comparado con el radio de Larmor de los electrones, permite una recolección eficiente de electrones en el régimen OML (Orbital Motion Limited) de sondas de Langmuir. La corriente dada por la teoría OML varía en función del perímetro y la longitud. En el caso de una cinta delgada, el perímetro depende de la anchura, que debe ser suficientemente grande para evitar cortes producidos por debris y micrometeoritos, y suficientemente pequeño para que la amarra funcione en dicho régimen [95]. En el experimento espacial TSS-1R mencionado anteriormente, se identificó una recolección de corriente más elevada que la que predecía el modelo teórico de Parker- Murphy, debido posiblemente a que se utilizaba un colector esférico de radio bastante mayor que la longitud de Debye [79]. En el caso de una amarra “bare”, que recoge electrones a lo largo de gran parte de su longitud, se puede producir un fenómeno conocido como atrapamiento adiabático de electrones (adiabatic electron trapping) [25, 40, 60, 73, 74, 97]. En el caso terrestre (LEO) se da la condición mesotérmica en la que la amarra se mueve con una velocidad muy superior a la velocidad térmica de los iones del ambiente y muy inferior a la velocidad térmica de los electrones. J. Laframboise y L. Parker [57] mostraron que, para una función de distribución quasi-isotrópica, la densidad de electrones debe entonces ser necesariamente inferior a la densidad ambiente. Por otra parte, debido a su flujo hipersónico y a la alta polarización positiva de la amarra, la densidad de los iones es mayor que la densidad ambiente en una vasta región de la parte “ram” del flujo, violando la condición de cuasi-neutralidad,-en una región de dimensión mayor que la longitud de Debye-. La solución a esta paradoja podría basarse en el atrapamiento adiabático de electrones ambiente en órbitas acotadas entorno al tether. ABSTRACT New technological and scientific applications by electrodynamic tethers for planetary missions are analyzed: i) A set of cylindrical, parallel tethers (electric solar sail or e-sail) is considered for an interplanetary mission; ions from the solar wind are repelled by the high potential of the tether, providing momentum to the e-sail. An approximated model of a stationary potential for a high solar wind flow is considered. With the force provided by a negative biased tether, an indirect method for the optimization trajectory of an Earth-to-Jupiter orbit transfer is analyzed. ii) The deployment of a tether from the e-sail allows several scientific applications in Jupiter. iia) It might be used as a source of radiative waves for plasma diagnostics and artificial aurora generator. A conductive tether orbiting in the Jovian magnetosphere produces waves. Wave radiation by a conductor carrying a steady current in both a polar, highly eccentric, low perijove orbit, as in NASA’s Juno mission, and an equatorial low Jovian orbit (LJO) mission below the intense radiation belts, is considered. Both missions will need electric power generation for scientific instruments and communication systems. Tethers generate power more efficiently than solar panels or radioisotope power systems (RPS). The radiation impedance is required to determine the current in the overall tether circuit. In a cold plasma model, radiation occurs mainly in the Alfven and fast magnetosonic modes, exhibiting a large refraction index. The radiation impedance of insulated tethers is determined for both modes and either mission. Unlike the Earth ionospheric case, the low-density, highly magnetized Jovian plasma makes the electron gyrofrequency much larger than the plasma frequency; this substantially modifies the power spectrum for either mode by increasing the Alfven velocity. An estimation of the radiation impedance of bare tethers is also considered. iib) In LJO, a spacecraft orbiting in a slow downward spiral under the radiation belts would allow determining magnetic field structure and atmospheric composition for understanding the formation, evolution, and structure of Jupiter. Additionally, if the cathodic contactor is switched off, a tether floats electrically, allowing e-beam emission that generate auroras. On/off switching produces bias/current pulses and signal emission, which might be used for Jovian plasma diagnostics. In LJO, the ions impacting against the negative-biased tether do produce secondary electrons, which racing down Jupiter’s magnetic field lines, reach the upper atmosphere. The energetic electrons there generate auroral effects. Regions where the tether efficiently should produce secondary electrons are analyzed. iic) Other scientific application suggested in LJO is the in-situ detection of charged grains. Charged grains naturally orbit near Jupiter. High-energy electrons in the Jovian ambient may be modeled by the kappa distribution function. In complex plasma scenarios, where the Jovian high electric field may accelerate charges up superthermal velocities, the use of non-Maxwellian distributions should be considered. In these cases, the distribution tails fit well to a power-law dependence for electrons. Fluctuations of the charged grains for non-Mawellian distribution function are here studied. iii) The present thesis is concluded with the analysis for de-orbiting satellites at end of mission by electrodynamic tethers. A de-orbit tether system must present very small tether-to-satellite mass ratio and small probability of a tether cut by small debris too. The present work shows how to select tape dimensions so as to minimize the product of those two magnitudes. Preliminary results of tape-tether design are here discussed to minimize that function. Results for de-orbiting Cryosat and Envisat are also presented.

Imaging of H217O distribution in the brain of a live rat by using proton-detected 17O MRI

Imaging of H217O has a number of important applications. Mapping the distribution of H217O produced by oxidative metabolism of 17O-enriched oxygen gas may lead to a new method of metabolic functional imaging; regional cerebral blood flow also can be measured by measuring the H217O distribution after the injection of 17O-enriched physiological saline solution. Previous studies have proposed a method for indirect detection of 17O. The method is based on the shortening of the proton T2 in H217O solutions, caused by the residual 17O-1H scalar coupling and transferred to the bulk water via fast chemical exchange. It has been shown that the proton T2 of H217O solutions can be restored to that of H216O by irradiating the resonance frequency of the 17O nucleus. The indirect 17O image thus is obtained by taking the difference between two T2-weighted spin-echo images: one acquired after irradiation of the 17O resonance and one acquired without irradiation. It also has been established that, at relatively low concentrations of H217O, the indirect method yields an image that quantitatively reflects the H217O distribution in the sample. The method is referred to as PRIMO (proton imaging of oxygen). In this work, we show in vivo proton images of the H217O distribution in a rat brain after an i.v. injection of H217O-enriched physiological saline solution. Implementing the indirect detection method in an echo-planar imaging sequence enabled obtaining H217O images with good spatial and temporal resolution of few seconds.

Antichagásicos potenciais: síntese e modelagem molecular de híbridos de hidrazonas e liberadores de óxido nítrico

A doença de Chagas é uma parasitose extremamente negligenciada, cujo agente etiológico é o protozoário Trypanosoma cruzi. Atualmente, 21 países da América Latina são considerados regiões endêmicas, onde 75-90 milhões de pessoas estão expostas à infecção, 6-7 milhões estão infectadas e mais de 41 mil novos casos surgem por ano. Entretanto, apenas os fármacos nifurtimox e benznidazol estão disponíveis no mercado. Estes, além da baixa eficácia na fase crônica da parasitose, apresentam diversos efeitos adversos, sendo que no Brasil apenas o benznidazol é utilizado. Este fato mostra a importância de se ampliar o número de fármacos disponíveis e propor quimioterapia mais eficaz para o tratamento da doença de Chagas. Como forma de contribuir para essa busca, este trabalho objetiva a síntese de compostos híbridos bioisostéricos N-acilidrazônicos e sulfonilidrazônicos, contendo grupo liberador de óxido nítrico, com potencial de interação com cisteíno-proteases parasitárias, tais como a cruzaína. Nestes derivados, os grupos liberadores de óxido nítrico utilizados foram os grupos furoxano (contendo substituinte metílico e fenílico) e éster nitrato. Propôs-se a variação de anéis aromáticos substituídos e não-substituídos, com o intuito de avaliar a possível relação estrutura-atividade (REA) desses análogos. Até o momento, somente os compostos da série N-acilidrazônica tiveram avaliação biológica realizada. Os valores de IC50 dos compostos na forma amastigota do parasita variaram entre >100 a 2,88 µM, sendo este último valor comparável ao fármaco de referência. A atividade inibitória frente à cruzaína foi de 25,2 µM a 2,2 µM. Já a liberação de óxido nítrico foi avaliada pelo método indireto de detecção de nitrato e os valores variaram entre 52,0 µM e 4.232,0 µM. Estes são bem inferiores ao composto padrão, além de não se identificar correlação direta entre a atividade biológica e a liberação de NO. Na sequência, os dois compostos mais ativos (6 e 14) foram submetidos a estudos de permeabilidade e de citotoxicidade. O composto 6 foi considerado o de maior permeabilidade segundo o Sistema de Classificação Biofarmacêutica (SCB) e todos os compostos apresentaram a taxa de fluxo menor que 2, indicando a ausência de mecanismo de efluxo. Na avaliação do potencial citotóxico desses compostos em células humanas, o derivado 6 apresentou índice de seletividade superior ao do benznidazol. Em estudos de modelagem molecular usando análise exploratória de dados (HCA e PCA), propriedades estéricas/geométricas e eletrônicas foram consideradas as mais relevantes para a atividade biológica. Além disso, estudos de docking mostraram que a posição do grupo nitro no anel aromático é importante para a interação com a cruzaína. Ademais o composto 6 não provocou mudanças significativas no ciclo celular e na fragmentação de DNA em células humanas, mostrando-se como líder promissor para futuros estudos in vivo. Atividade tripanomicida, citotoxicidade, potencial de liberação de NO e estudos de permeabilidade dos 23 derivados sulfonilidrazônicos e ésteres nitrato estão sendo avaliados.

Rivalidad en el mercado español de depósitos bancarios: la teoría de los grupos estratégicos

El objetivo de este trabajo consiste en examinar la rivalidad intra y entre grupos estratégicos de una industria. La literatura sobre la materia sugiere que las evidencias empíricas detectadas pueden verse afectadas por problemas operativos relacionados con la medición de la rivalidad. Como novedad, la metodología aplicada propone la utilización de dos enfoques de medición de la rivalidad, uno indirecto mediante la estimación de la variación conjetural, y una evaluación directa a través de las noticias sobre acciones y reacciones estratégicas detectadas en las publicaciones. La aplicación empírica realizada en el mercado español de depósitos bancarios no permite asegurar nada acerca de la mayor o menor rivalidad intra que entre grupos; pero los grupos estratégicos permiten predecir la forma en que cada entidad compite con las demás.

Chemical kinetic investigation of a commercial batch reactor process

The aim of this investigation was to study the chemical reactions occurring during the batchwise production of a butylated melamine-formaldehyde resin, in order to optimise the efficiency and economics of the batch processes. The batch process models are largely empirical in nature as the reaction mechanism is unknown. The process chemistry and the commercial manufacturing method are described. A small scale system was established in glass and the ability to produce laboratory resins with the required quality was demonstrated, simulating the full scale plant. During further experiments the chemical reactions of methylolation, condensation and butylation were studied. The important process stages were identified and studied separately. The effects of variation of certain process parameters on the chemical reactions were also studied. A published model of methylolation was modified and used to simulate the methylolation stage. A major result of this project was the development of an indirect method for studying the condensation and butylation reactions occurring during the dehydration and acid reaction stages, as direct quantitative methods were not available. A mass balance method was devised for this purpose and used to collect experimental data. The reaction scheme was verified using this data. The reactions stages were simulated using an empirical model. This has revealed new information regarding the mechanism and kinetics of the reactions. Laboratory results were shown to be comparable with plant scale results. This work has improved the understanding of the batch process, which can be used to improve product consistency. Future work has been identified and recommended to produce an optimum process and plant design to reduce the batch time.

Multi-frequency segmental bio-impedance device:design, development and applications

Bio-impedance analysis (BIA) provides a rapid, non-invasive technique for body composition estimation. BIA offers a convenient alternative to standard techniques such as MRI, CT scan or DEXA scan for selected types of body composition analysis. The accuracy of BIA is limited because it is an indirect method of composition analysis. It relies on linear relationships between measured impedance and morphological parameters such as height and weight to derive estimates. To overcome these underlying limitations of BIA, a multi-frequency segmental bio-impedance device was constructed through a series of iterative enhancements and improvements of existing BIA instrumentation. Key features of the design included an easy to construct current-source and compact PCB design. The final device was trialled with 22 human volunteers and measured impedance was compared against body composition estimates obtained by DEXA scan. This enabled the development of newer techniques to make BIA predictions. To add a ‘visual aspect’ to BIA, volunteers were scanned in 3D using an inexpensive scattered light gadget (Xbox Kinect controller) and 3D volumes of their limbs were compared with BIA measurements to further improve BIA predictions. A three-stage digital filtering scheme was also implemented to enable extraction of heart-rate data from recorded bio-electrical signals. Additionally modifications have been introduced to measure change in bio-impedance with motion, this could be adapted to further improve accuracy and veracity for limb composition analysis. The findings in this thesis aim to give new direction to the prediction of body composition using BIA. The design development and refinement applied to BIA in this research programme suggest new opportunities to enhance the accuracy and clinical utility of BIA for the prediction of body composition analysis. In particular, the use of bio-impedance to predict limb volumes which would provide an additional metric for body composition measurement and help distinguish between fat and muscle content.

Análise de tendência e caracterização sazonal e interanual da evapotranspiração de referência para o sudoeste da amazônia brasileira: Acre, Brasil

The objective of this research was to investigate monthly climatological, seasonal, annual and interdecadal of the reference evapotranspiration (ETo) in Acre state in order to better understand its spatial and temporal variability and identify possible trends in the region. The study was conducted with data from Rio Branco municipalities, the state capital, Tarauacá and Cruzeiro do Sul considering a 30-year period (1985-2014), from monthly data from weather stations surface of the National Institute of Meteorology. The methodology was held, first, the consistency of meteorological data. Thus, it was made the gap filling in the time series by means of multivariate techniques. Subsequently were performed statistical tests trend (Mann-Kendall) and homogeneity, by Sen's estimator of the magnitude of this trend is estimated, as well as computational algorithms containing parametric and non-parametric tests for two samples to identify from that year the trend has become significant. Finally, analysis of variance technique (ANOVA) was adopted in order to verify whether there were significant differences in average annual evapotranspiration between locations. The indirect method of Penman-Montheith parameterized by FAO was used to calculate the ETo. The results of this work through examination of the descriptive statistics showed that the ETo the annual average was 3.80, 2.92 and 2.86 mm day-1 year, to Rio Branco, Tarauacá and Cruzeiro do Sul, respectively. Featuring quite remarkable seasonal pattern with a minimum in June and a maximum in October, with Rio Branco to town one with the strongest signal (amplitudes) on the other hand, the Southern Cross presented the highest variability among the studied locations. By ANOVA it was found that the average annual statistically different for a significance level of 1% between locations, but the annual average between Cruzeiro do Sul and Tarauacá no statistically significant differences. For the three locations, the 2000s was the one with the highest ETo values associated with warmer waters of the North Atlantic basin and the 80s to lower values, associated with cooler waters of this basin. By analyzing the Mann-kendall and Sen estimator test, there was a trend of increasing the seasonal reference evapotranspiration (fall, winter and spring) on the order of 0.11 mm per decade and that from the years of 1990, 1996 and 2001 became statistically significant to the localities of Cruzeiro do Sul Tarauacá and Rio Branco, respectively. For trend analysis of meteorological parameters was observed positive trend in the 5% level of significance, for average temperature, minimum temperature and solar radiation.

Design construction and testing of a heat loop for study of fouling and its interrelationship to corrosion in heat exchanger tubes

A heat loop suitable for the study of thermal fouling and its relationship to corrosion processes was designed, constructed and tested. The design adopted was an improvement over those used by such investigators as Hopkins and the Heat Transfer Research Institute in that very low levels of fouling could be detected accurately, the heat transfer surface could be readily removed for examination and the chemistry of the environment could be carefully monitored and controlled. In addition, an indirect method of electrical heating of the heat transfer surface was employed to eliminate magnetic and electric effects which result when direct resistance heating is employed to a test section. The testing of the loop was done using a 316 stainless steel test section and a suspension of ferric oxide and water in an attempt to duplicate the results obtained by Hopkins. Two types of thermal ·fouling resistance versus time curves were obtained . (i) Asymptotic type fouling curve, similar to the fouling behaviour described by Kern and Seaton and other investigators, was the most frequent type of fouling curve obtained. Thermal fouling occurred at a steadily decreasing rate before reaching a final asymptotic value. (ii) If an asymptotically fouled tube was cooled with rapid cir- ·culation for periods up to eight hours at zero heat flux, and heating restarted, fouling recommenced at a high linear rate. The fouling results obtained were observed to be similar and 1n agreement with the fouling behaviour reported previously by Hopkins and it was possible to duplicate quite closely the previous results . This supports the contention of Hopkins that the fouling results obtained were due to a crevice corrosion process and not an artifact of that heat loop which might have caused electrical and magnetic effects influencing the fouling. The effects of Reynolds number and heat flux on the asymptotic fouling resistance have been determined. A single experiment to study the effect of oxygen concentration has been carried out. The ferric oxide concentration for most of the fouling trials was standardized at 2400 ppM and the range of Reynolds number and heat flux for the study was 11000-29500 and 89-121 KW/M², respectively.

Paisagem, um patrimônio esquecido: subsídios para a construção de uma metodologia de valoração e proteção do cenário natural litorâneo

The so-called "residential tourism" came to intensify the previous occupation of the coastal zones, characterized by traditional beach houses, and brought significant consequences for their spatial configuration, and especially for its scenic value of the landscape. Although there is the intention to regulate the activities of enterprising groups by some legal instruments to control the use and occupation of land, and to contain some negative effects, the actions of government are still inefficient in trying to follow the implications on the landscape from the accelerated growth of the real estate and touristic sectors. Supported in the speech of economic development and income generation, public managers prioritize areas to attract tourists to the detriment of preserving important physical attributes of the natural environment that contribute significantly to the quality of life. The result can be noticed in the use of natural elements as one of the major components in the land valorization, and in the immediate attraction of investors and enterprising. Therefore, the objective of this work is to contribute to the debate on the landscape preservation a little detailed thematic in view of their relevance in the current context - by indicating subsidies to the creation of a methodology for the evaluation and protection of coastal zones that may assist the government in creating new instruments, and better prepare it in control of the occupation of these areas. For such, was followed two ways to analysis: the indirect method and the direct method. The first is grounded in the evaluation of landscape attributes, which is based on the work of Raquel Tardin (2008) and Eduardo Cuesta, Encarnación Algarra and Isabel Pastor (2001). The second, based on the research of Leticia and Carlos Hardt (2010) and on the concepts of phenomenology - expressed by Antonio Christofoletti (1985) and Yi-Fu Tuan (1983) - considers the population perspective on the quality of the natural scenery. Developed through cartographic materials, photographic collections and quantitative tables, this dissertation utilized as a case study the beaches of Barra de Tabatinga and Camurupim, located in the city of Nísia Floresta/RN. Despite already being sighted spaces of advanced stage of landscape degradation in these locations, areas of remarkable scenic value can still be found, what reinforce the urgency in adopting preservationists actions. The absence of laws focused on the management and protection of the landscape singularities associated with the inefficiency of the government to invigilate the land occupation in coastal zones, encourage the excessive action of the real estate-tourism, and consequently make the government the main responsible for the environmental and landscape impacts in these areas - by its omission or by their connivance. Therefore, the legislation permeates this entire process and constitutes itself as the most effective way to guarantee the right to the landscape to present and future generations. Are also pointed some important considerations to build a methodology, especially concerning possibilities of improvements and adaptations of its applicability in each case