474 resultados para E3
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12 p.
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6molXZJudd-OfeltEr^3AJJp
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Er^3Yb^3La2O3sYb^3Er^35at05ats=965ms
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Background Ubiquitination is known to regulate physiological neuronal functions as well as to be involved in a number of neuronal diseases. Several ubiquitin proteomic approaches have been developed during the last decade but, as they have been mostly applied to non-neuronal cell culture, very little is yet known about neuronal ubiquitination pathways in vivo. Methodology/Principal Findings Using an in vivo biotinylation strategy we have isolated and identified the ubiquitinated proteome in neurons both for the developing embryonic brain and for the adult eye of Drosophila melanogaster. Bioinformatic comparison of both datasets indicates a significant difference on the ubiquitin substrates, which logically correlates with the processes that are most active at each of the developmental stages. Detection within the isolated material of two ubiquitin E3 ligases, Parkin and Ube3a, indicates their ubiquitinating activity on the studied tissues. Further identification of the proteins that do accumulate upon interference with the proteasomal degradative pathway provides an indication of the proteins that are targeted for clearance in neurons. Last, we report the proof-of-principle validation of two lysine residues required for nSyb ubiquitination. Conclusions/Significance These data cast light on the differential and common ubiquitination pathways between the embryonic and adult neurons, and hence will contribute to the understanding of the mechanisms by which neuronal function is regulated. The in vivo biotinylation methodology described here complements other approaches for ubiquitome study and offers unique advantages, and is poised to provide further insight into disease mechanisms related to the ubiquitin proteasome system.
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Cannabinoid CB1 receptors peripherally modulate energy metabolism. Here, we investigated the role of CB1 receptors in the expression of glucose/pyruvate/tricarboxylic acid (TCA) metabolism in rat abdominal muscle. Dihydrolipoamide dehydrogenase (DLD), a flavoprotein component (E3) of alpha-ketoacid dehydrogenase complexes with diaphorase activity in mitochondria, was specifically analyzed. After assessing the effectiveness of the CB1 receptor antagonist AM251 (3 mg kg(-1), 14 days) on food intake and body weight, we could identified seven key enzymes from either glycolytic pathway or TCA cycle-regulated by both diet and CB1 receptor activity-through comprehensive proteomic approaches involving two-dimensional electrophoresis and MALDI-TOF/LC-ESI trap mass spectrometry. These enzymes were glucose 6-phosphate isomerase (GPI), triosephosphate isomerase (TPI), enolase (Eno3), lactate dehydrogenase (LDHa), glyoxalase-1 (Glo1) and the mitochondrial DLD, whose expressions were modified by AM251 in hypercaloric diet-induced obesity. Specifically, AM251 blocked high-carbohydrate diet (HCD)-induced expression of GPI, TPI, Eno3 and LDHa, suggesting a down-regulation of glucose/pyruvate/lactate pathways under glucose availability. AM251 reversed the HCD-inhibited expression of Glo1 and DLD in the muscle, and the DLD and CB1 receptor expression in the mitochondrial fraction. Interestingly, we identified the presence of CB1 receptors at the membrane of striate muscle mitochondria. DLD over-expression was confirmed in muscle of CB1-/- mice. AM251 increased the pyruvate dehydrogenase and glutathione reductase activity in C2C12 myotubes, and the diaphorase/oxidative activity in the mitochondria fraction. These results indicated an up-regulation of methylglyoxal and TCA cycle activity. Findings suggest that CB1 receptors in muscle modulate glucose/pyruvate/lactate pathways and mitochondrial oxidative activity by targeting DLD.
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[Ipomoea batatm L.]l8Ms2mgl2.4-DIIVDNAIMslVSDS-PAGEIRAPD10G3(GAGCCCTCCA)I IPCVPH2.4-DMSImg/12.4-D2.4-D4-8mgl0.5mg/l2.4-D2.4-D2.4-D2.4-D PH6.0E32200.5mg/1 2.4-D0.5mg/12.4-DImg/16-BA (Bt)PCRBt
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(GCTE)(IGBP)(Terrestrial Transect)GCTEIGBP(Cyclobalanopsisgtauco);Kira;;IGBP;r 1. KoppenBoxPenmanThornthwaite, HoldridgeBudykoKiraKoppenThornthwaiteHoldridge. Kira. Box. Neilson.Woodward, Budyko, PrenticeHoldridgeUvardyMatthews. Olson. BaileyWoodward.Prentice. BoxC02 2. [Cyclobalanopsis glauca (Thunb.) Oerst.]KiraPenmanThomthwaiteHoldridge (TEMP). 1(Tl)7(T7)(TMAX)(TMIN).10(AT)(PREC)KirarHoldridgeC02 3. 1 689EIS7.26,0 KaB.P.2100 mm;; 2KIRA Kira510BWIBCIBK6891 0;; 3 -689826;; 4. Biome()9 5. (TMrN)(TJAN)(MXT)(MIT);(AT);(PJAN)(PJUL)(PSD)(PCV);(VEGET)(VEGED)(NPP)(FLORA)(FA UNA).(EDGENUS)(GENUSSPECIES);(SOILT)(SOILPH)(SOILEXC);(LONG)(LAT)(ALT)(biodomain) -(subbiodomain) -(biome) -(bioregion).571 8 I I A I Al IA2 II II B II Bl II B2 il C II Cl C2 III III D m DI III E III El III E2 I E3 III E4 IV IV F IV Fl IV F2 V vGV GlV G2V G3V G4V GsIGBP (IGBP)(Interproject)(Terrestrial Transect)IGBP1000 km(GCM)IGBPIGBP;;;PAGESPEP 6.(N ECT) -(IGBP):-(NECT)IGBP112013003043030l 600 km3NECT(GCTE)IGBP 7.
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The present study was carried out in order to establish an economical effective diet for the pacific white shrimp in the southern part conditions of Iran. With the consideration of three dietary energy levels (E1=262, E2=312, E3=362 kcal 100 g-1 diet) and six ratios of fish meal (FM) to soybean meal (SBM) [(P1=100%FM+0%SBM, P2=80%FM+20%SBM, P3=60%FM+40%SBM, P4=40%FM+60%SBM, P5=20%FM+80%SBM, P6=0%FM+100%SBM)], 18 experimental diets (with 36% crude protein) were prepared. Completely randomized design was used to assign 54 polyethylene 300 litre round tanks provided by aeration and flow through water system and was stocked by 19 juvenile as 3 replicates to each treatment. Shrimps average weight was about 0.77 grams at the start. After 56 days culture period, maximum growth and nutritional performances were observed in the P6E1 treatment (containing 100% soybean meal and 262 kcal 100 g-1 diet) and P5E1 treatment (containing 80% soybean meal and 262 kcal 100 g-1 diet). Also the highest survival rate of the shrimps was observed in the P1E1, P1E2, P3E3 and P5E3 treatments. Additionally interactive effect of different protein ratios and energy levels had significant difference on body protein, fat, fiber and ash contents (P<0.05). Results of the present study suggest the possibility replacement of at least 80% of dietary fish meal by soybean meal in the diet of pacific white shrimp in the conditions of southern part of Iran.
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--(GC-MS)(OP)(NP)A(BPA)(DES)(E1)17β-(E2)17α-(EE2)(E3)8(65.4±4.0)%(110.0±4.5)%,1.07.5ng/L(5.2%15.6%),(EE2)6.58954.9ng/L;EE2E3,3.22
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Con-elation between nc-Si, Er3+ and nonradiative defects in Er-doped nc-Si/SiO2 films is studied. Upon the 514.5 run laser excitation, the samples exhibit a nanocrystal-related spectrum centered at around 750 nm and an Er3+ luminescence line at 1.54mum. With increasing Er3+ content in the films,the Er3+ emission becomes intense while the photoluminescence at 750 nm decreases. Hydrogen passivation of the samples is shown to result in increases of the two luminescence peaks. However, the effect of hydrogen treatment is different for the samples annealed at different temperatures. The experimental results show that the coupling between Er3+, nc-Si and noradiative centers has a great influence on photoluminescence from nc-Si/SiO2 < Er > films.
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UPSUPS xRNF220-likemRNF220 xRNF220 E3 mRNF220 Sin3B Sin3B UPS mRNF220 Sin3B morpholino xRNF220-like Sin3B mRNF220 Sin3B Sin3/HDAC RNF220 RNF220-like HCA127 HCA127 HCA127 HCA127 RING UPS HCA127 ASPP2 ASPP2 p53 ASPP2 N-tubulin Slug ASPP2
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The deep centers in AlGaAs/GaAs graded index-separate confinement heterostructure single quantum well (GRIN-SCHSQW) laser structures grown by MBE and MOCVD have been investigated using deep level transient spectroscopy (DLTS) technique, The majority and minority carrier DLTS spectra show that the deep (hole and electron) traps (Hi and E3), having large capture cross sections and concentrations, are observed in the graded n-AlxGa1-xAs layer of laser structures in addition to the well-known DX centers. For laser structures grown by MBE, the deep hole trap H1 and the deep electron trap E3 may be spatially localized in the interface regions of discontinuous variation Al mole fraction of the n-AlxGa1-xAs layer with x = 0.20-0.43. For laser structures grown by MOCVD, the deep electron trap E3 may be spatially localized in the n-AlxGa1-xAs layer with x = 0.18-0.30, and the DX center may be spatially localized in the interface regions of discontinuous variation Al mole fraction of the AlxGa1-xAs layer with x = 0.22-0.30.
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6B1B2E3E4 (E5E6B2E4, E625Kb46KDaRNA0. 45Kb E446KDa,120KDapH7.0,29 pH40H_2O_20.4%80uM40uM0.1mM
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(Fraxinus sogdiana)4(E1-(Hebeloma mesophaeusm)E2-(Lactarius insulsns)E3-(Stro-bilomyces floccopus)E4-(Cortinarius russus):,,23.6%;E1E3E2E4,21.0%12.7%,,,E1E3E2E4100%67%81%,,
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Iodothyronine plays a major role in growth, basic metabolism and organ formation. It has an extremely limited source in the body. In this thesis, we designed iodothyronine(T4) as hapten. Then a single chain antibody displayed on phange was obtained from a human phage displaying a single chain antibody library. The specific genes of E3 was subcloned in P-5E vector. According to its amino acid sequences, we simulate its three dimention structure by computer. It has never been reported in PDB.
