Transcription rates of yeast genes are influenced by the distribution of introns

A comparative analysis on the intron sequence oligonucleotide usages in two sets of yeast genes with higher and lower transcription frequencies, respectively, has shown that the intron sequence structures of the two sets of genes are different. There are more potential binding sites for transcription factors in the introns of the genes with high transcription frequencies. So it is speculated that introns regulate the transcription of genes. But more evidences are needed to favor this speculation. The detailed comparative analyses on the distribution ( length and position) of introns and exons in the two sets of gene sequences also show that there is an obvious boundary between the lengths of the two sets of introns. There is no boundary between the lengths of the two sets of exons, although the means of their lengths are of discrepancy. The situation of the gene lengths ( length of intron and exon) is similar to exon lengths. As far as the relative position, the introns in two sets of genes all have a bias toward the 5' ends of genes. But as the actual position is considered, more introns in high transcription genes have a tendency to be located toward the 5' ends of genes, some even located at 5'-UTR. These results suggest that the gene transcription rates are related to the length of intron, but not to the lengths of exons and genes sequences. The positions of introns may also influence the transcription rates. The transcriptional regulation of introns may be correlative with the transcriptional regulation of the upstream of genes, or be its continuous action.

Detection of Construction Workers in Video Frames for Automatic Initialization of Vision Trackers

Vision based tracking can provide the spatial location of construction entities such as equipment, workers, and materials in large scale, congested construction sites. It tracks entities in video streams by inferring their locations based on the entities’ visual features and motion histories. To initiate the process, it is necessary to determine the pixel areas corresponding to the construction entities to be tracked in the following consecutive video frames. In order to fully automate the process, an automated way of initialization is needed. This paper presents the method for construction worker detection which can automatically recognize and localize construction workers in video frames. The method first finds the foreground areas of moving objects using a background subtraction method. Within these foreground areas, construction workers are recognized based on the histogram of oriented gradients (HOG) and histogram of the HSV colors. HOG’s have proved to work effectively for detection of people, and the histogram of HSV colors helps differentiate between pedestrians and construction workers wearing safety vests. Preliminary experiments show that the proposed method has the potential to automate the initialization process of vision based tracking.

柠檬酸对高岭石的溶解作用

采用间歇法(batch method)研究柠檬酸对高岭石溶解的影响。结果表明:低浓度(0.02mmolL-1)柠檬酸可促进高岭石中Ca2+、Mg2+、Na+少量的释放。当柠檬酸浓度分别为1.0mmolL-1和8mmolL-1时,Ca2+,Mg2+,K+,Na+,Si,Al3+阳离子的释放量也相应地增加。释放的Ca2+、Mg2+主要来自高岭石表面的吸附,而Na+、K+则来自高岭石的溶解。

A trichordal temporal approach to digital coordination: the sociomaterial mangling of the CERN grid

This paper develops a sociomaterial perspective on digital coordination. It extends Pickering’s mangle of practice by using a trichordal approach to temporal emergence. We provide new understanding as to how the nonhuman and human agencies involved in coordination are embedded in the past, present, and future. We draw on an in-depth field study conducted between 2006 and 2010 of the development, introduction, and use of a computing grid infrastructure by the CERN particle physics community. Three coordination tensions are identified at different temporal dimensions, namelyobtaining adequate transparency in the present, modeling a future infrastructure, and the historical disciplining of social and material inertias. We propose and develop the concept of digital coordination, and contribute a trichordal temporal approach to understanding the development and use of digital infrastructure as being orientated to the past and future while emerging in the present.

Analysis of heavy metals of muscle and intestine tissue in fish - in Banan section of Chongqing from Three Gorges Reservoir, China

The present study was carried out to investigate contamination of heavy metals in 19 fish species from the Banan section of Chongqing in the Three Gorges, Yangtze River. The results showed that the mean concentrations of heavy metals were higher in intestine than muscle, except zinc in upper strata. In the fish inhabiting the upper strata, there were significant differences between mean concentrations of As, Cr, Cu and Hg in muscle and intestine (P <0.05). There were also significant differences between mean concentrations of Cr and Cu in muscle and intestine in the fish inhabiting middle strata. However, significant differences between mean concentrations of As, Cd, Hg, Pb and Zn were measured in fish inhabiting bottom strata in both intestine and muscle tissues (P <0.05). For the fish inhabiting different strata, the concentrations of As, Cd, Cr, Cu, Hg and Ph in muscle and intestine of the fish from bottom strata (BS) were higher than those in both upper strata (US) and middle strata (MS); whereas a higher concentration of Zn was measured in muscle and intestine from fish inhabiting upper strata. Mean metal concentrations were found to be higher in age 11 than those in age I in Coreius heterodon (2- and 1-year odl fish respectively). The overall results indicated that fish muscle in the Banan section were slightly contaminated by heavy metals, but did not exceed Chinese food standards.

Graphene saturable absorbers for VECSELs

We report mode-locking of an optically pumped VECSEL using a graphene-based saturable absorber mirror (GSAM). Self-starting and stable modelocked operation is demonstrated with 473 fs pulses at 1.5 GHz repetition rate and 949 nm center wavelength. Wavelength tuning is achieved over a 46 nm bandwidth. We discuss the mirror design, the fabrication of the GSAMs, and give an outlook on further optimization of the design, including dielectric top coatings to protect the graphene and to increase the flexibility in the design. © 2014 SPIE.

Water solubility enhancement of phthalates by cetyltrimethylammonium bromide and beta-cyclodextrin

Water solubility enhancements of six phthalates (five aliphatic phthalates and one phenyl phthalate) by cetyltrimethylammonium bromide (CTAB) and beta-cyclodextrin (beta-CD) were studied at 25 degreesC. The solubilities of these plithalates are remarkably enhanced by CTAB solutions above the critical micelle concentration (cmc). Only marginal enhancement of phthalate solubility was observed in solutions containing CTAB below its cmc and beta-CD at low concentrations (less than 5 mM). The solubility enhancements of the plithalates are proportional to the added amount of CTAB and beta-CD. Partition coefficients of the plithalates between monomeric CTAB surfactant and water (K-MN) and between CTAB micelle and water K-MC) were estimated from the experimental data. The mechanisms of solubility enhancements by CTAB and beta-CD were discussed. A log-linear equation was proposed and evaluated for the solubilization by CTAB below cmc, while the previously proposed linear partitioning model was questioned. The structures of the complexes formed between plithalates and beta-CD were proposed, and the formation constants were estimated. The values of log K-MC, log K-MN, and log Kbeta-CD of the plithalates were found to correlate linearly with the log K-OW of plithalates, with the exception of the solid phenyl phthalate.

Electronic structure of self-assembled InAs quantum discs in a magnetic field with varying orientation and two-electron quantum-disc qubits

We have studied the electronic structure of vertically assembled quantum discs in a magnetic field with varying orientation using the effective mass approximation. We calculate the four energy levels of single-electron quantum discs and the two lowest energy levels of two-electron quantum discs in a magnetic field with varying orientation. The change of the magnetic field as an effective potential strongly modifies the electronic structure, leading to splittings of the levels and anticrossings between the levels. The calculated results also demonstrate the switching between the ground states with the total spin S = 0 and 1. The switching induces a qubit controlled by varying the orientation of the magnetic field.

中药处方配伍监控系统的设计与实现

医院信息系统(hospital information system,HIS)作为医院信息化建设的重要组成部分越来越受到社会的重视,已经进入一个高速发展的阶段。但是作为诊疗过程的一个重要组成部分-药物处方治疗,在HIS中却缺少必要的监控与指导。在HIS标准化推进的同时,更应该对其深度和广度进行研究,结合软件工程的设计思想,基于面向对象的开发方法,设计开发了“中药处方配伍”监控系统,提供了临床用药指导功能,使其作为HIS的一个子系统能够实时的对医生处方进行监控,使临床医药人员记得更多、更准、更细,以降低药源性疾病,提高用药质量,加强用药管理,避免医疗纠纷。

Petri网可达性的综合判定法

Petri网标识的可达性判定问题是进行Petri网分析的基础,而传统的判定方法并不能确保所得结果的可靠性.在揭示Petri网可达性问题的实质之后,讨论了在标识图的同一连通域内标识可达性的判定问题,进而在分析相关原理的基础上提出了一种有效判定Petri网标识可达性的综合判定法.此判定方法综合多种传统判定方法的优点,结合Gr鯾ner基理论,确保了对Petri网标识可达性进行判定所得结果的可靠性.

处理二维定常流中交界面的座标分裂解法

本文通过建立座标分裂的概念，成功地解决了二维非定常流中交界面的问题，该方法同时具有简单、精度高和计算速度快等优点。本文给出了气波增压器转子内部流场的计算结果、并和现有的其它计算结果作了比较。

闪耀光栅外腔反馈压窄半导体激光器线宽技术的研究

在讨论半导体激光线宽压窄理论的基础上,利用闪耀光栅作为外部反馈元件,介绍了由中心波长为949.6nm、原始线宽为1.2THz的单管半导体激光器构成的反馈外腔,它能够很好的改善半导体激光器的性能.实验得到了中心波长稳定的、单纵模的高质量激光输出,边模抑制比大于30dB,线宽优于1.2MHz (Δλ＜3.6×10~(-6)nm).实验证实了强反馈能够很好地改善外腔半导体激光器的动态特性.