Age-related changes in mouse bone permeability

The determination of lacunar-canalicular permeability is essential for understanding local fluid flow in bone, which may indicate how bone senses changes in the mechanical environment to regulate mechano-adaptation. The estimates of lacunar-canalicular permeability found in the literature vary by up to eight orders of magnitude, and age-related permeability changes have not been measured in non-osteonal mouse bone. The objective of this study is to use a poroelastic approach based on nanoindentation data to characterize lacunar-canalicular permeability in murine bone as a function of age. Nine wild type C57BL/6 mice of different ages (2, 7 and 12 months) were used. Three tibiae from each age group were embedded in epoxy resin, cut in half and indented in the longitudinal direction in the mid-cortex using two spherical fluid indenter tips (R=238 μm and 500 μm). Results suggest that the lacunar-canalicular intrinsic permeability of mouse bone decreases from 2 to 7 months, with no significant changes from 7 to 12 months. The large indenter tip imposed larger contact sizes and sampled larger ranges of permeabilities, particularly for the old bone. This age-related difference in the distribution was not seen for indents with the smaller radius tip. We conclude that the small tip effectively measured lacunar-canalicular permeability, while larger tip indents were influenced by vascular permeability. Exploring the age-related changes in permeability of bone measured by nanoindentation will lead to a better understanding of the role of fluid flow in mechano-transduction. This understanding may help indicate alterations in bone adaptation and remodeling. © 2013 Elsevier Ltd.

Induction and preliminary characterization of a novel halophage SNJ1 from lysogenic Natrinema sp F5

Halophage SNJ1 was induced with mitomycin C from Natrinema sp. strain F5. The phage produces plaques on Natrinema sp. strain J7 only. The phage has a head of about 67 nm in diameter and a tail of 570 nm in length and belongs morphologically to the family Siphoviridae. The phage is strongly salt dependent; NaCl concentration affects the integrity of SNJ1, phage adsorption, and plaque formation. The optimal NaCl concentration for phage adsorption and plaque formation is 30% and 25%, respectively.

Continuous speech research based on HyperSausage Neuron

In this paper, we presents HyperSausage Neuron based on the High-Dimension Space(HDS), and proposes a new algorithm for speaker independent continuous digit speech recognition. At last, compared to HMM-based method, the recognition rate of HyperSausage Neuron method is higher than that of in HMM-based method.

Whispering-gallery-likel modes in square resonators

The mode frequencies and field distributions of whispering-gallery (WG)-like modes of square resonators are obtained analytically, which agree very well with the numerical results calculated by the FDTD technique and Pade approximation method. In the analysis, a perfect electric wall for the transverse magnetic mode or perfect magnetic wall for the transverse electric mode is assumed at the diagonals of the square resonators, which not only provides the transverse mode confinement, but also requires the longitudinal mode number to be an even integer. The WG-like modes of square resonators are nondegenerate modes with high-quality factors, which make them suitable for fabricating single-mode low-threshold semiconductor microcavity lasers.

Continuous speech research based on HyperSausage Neuron

In this paper, we presents HyperSausage Neuron based on the High-Dimension Space(HDS), and proposes a new algorithm for speaker independent continuous digit speech recognition. At last, compared to HMM-based method, the recognition rate of HyperSausage Neuron method is higher than that of in HMM-based method.

圆斑蝰蛇毒磷脂酶A_2蛋白活性表现和分子进化探讨及菜花烙铁头、烙铁头蛇毒中Jerdonase、TmF的研究

磷脂酶AZ（PLA2）是蛇毒中含量较为丰富的一类作用于梭酷键的酶。迄今为止，己有多种形式的PLA2从不同地域、不同种属的蛇毒中得以纯化并进行了较为系统的研究。其中，以VipoXin为代表的异二聚体形式PLA2较为引人注目，原因在于这种形式不同于此类蛋白家族中的诸多其它个体。目前，己经有许多关于此异二聚体PL凡生物学特性的报道，包括对此类形式存在原因、活性变化、结构表现、系统进化等方面的讨论。然而至今，这种以异二聚体形式存在的PLA2仅发现于几种蛙亚科（ViperinaeSubfamily）蛇种的蛇毒中，其中就包括我国台湾岛的圆斑蜂蛇台湾亚种（Doboiarusselliiformosensis），而蝮亚科（CrotaiinaeSubfamil）蛇种的蛇毒至今却没有此类报道。我国大陆西南端接壤东南亚，存在于云南、福建一带的圆斑蛙蛇隶属圆斑蛙蛇泰国亚种（Daboiarusselliisiamensis），那么这种蛇毒中是否也含有异二聚体形式的PLA2呢？本工作就此疑问对云南产圆斑蛙蛇泰国亚种（D.r.siamensis）蛇毒中的PLA2进行了研究，结果得到三个新的PLAZ，分别命名为DRS-PLA2-I、DRS-PLA2-II和DRS-PLA2-III。其中，DRS-PLA2-I的分子量为13864.06Da，理论pI为4.56，PLA2活性为12.35μmol/mg/min；DRS-PLA2-II的分子量为13635.99Da，理论pI为8.74，PLA2活性为8.76μmol／mg/min；DRS-PLA2-III的分子量为13619.80Da，理论厂为4.61，无PLA2活性。这三个蛋白酶N端的30个氨基酸残基恰好和三个阳性克隆的cDNA序列推导的蛋白序列吻合，结合已经报道的PLA2蛋白家族蛋白序列的保守性表现，我们可以断定它们之间存在对应关系。分子系统学分析表明DRS-PLA2-II和DRS-PLA2-III在进化关系上和蛙亚科的异二聚体PLA2关系较近，并且二者酶活性分别与异二聚体PLA2的Normalchain和Inhibitorchain相一致，只是没有发现类似Vipoxin形式的异二聚体结合蛋白。这些分析表明DRS-PLA2-nORS-PLA2-III类似圆斑蛙蛇台湾亚种（D.r.forlnos翻s沽）中的PV-4/RV-7，是PLA2异二聚体的一种特殊形式，在进化上滞后于VinOXin。另夕卜本工作还相继从云南产菜花烙铁头（Trimeresrusjerdonii）蛇毒和湖南产烙铁头（Trimeresurusmucrosquamatus）蛇毒中分离得到Jerdonase和TmF。前者为一个丝氨酸蛋白酶性质的、具有纤维蛋白原水解作用和激肤释放酶原水解作用双重活性表现的、高分子量的份五brinogenase，其活性表现可以被PMSF彻底抑制，而EDTA对此却没有影响。其它的几种抑制剂如大豆胰蛋白酶抑制剂、l-cysteine、DTT对Jerdonase的活性表现也有不同程度的影响。在Jerdonase的这些生化特性上中，分子量的大小和对纤维蛋白酶水解的特性这两方面有别于蛇毒中诸多其它来源的同类蛋白；后者T淤为一个舒缓激肚增强肤（BradykninPQtentiatingPePtide，BPP），电离质谱分析表明其分子量为1110.7Da。此小肚氨基酸序列为促进舒缓激肚（Bradki垃n，BK）诱导的豚鼠回肠纵行肌收缩的活力单位为（1.13±0.3）（m留L），T妊抑制血管紧张素转化酶（ACE）对BK水解的半数抑制剂量IC50为2μg。比较已报道的从Agkistrodon属和Bothrops属中纯化得到的BPP氨基酸序列发现：BPP的N端都是特征性的pGlu，C端为IIe-Pro-Pro，有高度的保守性。另外，TmF是Trimeresurus属中此类小肤的首次纯化。总之，本研究对国产的几种常见蛇毒中的几种常见蛋白多肤进行了一定程度的探讨和分析，和相同类别的其它蛋白、多肤比较可以看到，有许多相同的地方，也有许多不同的表现，研究结果为相应领域的深入研究提供资料和思路。

HIRFL-CSR实验环电子冷却装置磁场测量

在HIRFL-CSR实验环电子冷却装置上采用了独立的高精度螺线管串联产生纵向磁场的设计,获得很高的冷却段磁场平行度。使用霍尔片测量磁场的分布,使用磁针测磁方法测量冷却段磁场的磁轴偏角,并根据测量及计算结果对单个线圈磁轴进行微调。测量及调试结果表明,在施加电流为额定电流的一半时,冷却段磁感应强度为0.078T,剩余磁场小于2×10-4T,磁场不平行度小于1×10-4,达到了预期的设计目标。

利用GIS和RS确定长白山自然保护区森林景观分布的环境范围

在对遥感数据进行景观分类和对环境因子进行空间表达基础上 ,在地理信息系统的支持下 ,确定长白山自然保护区森林景观分布的环境 (包括年均温、年降水量、坡度和坡向 )范围 .结果表明 ,从苔原、岳桦、云冷杉到阔叶红松林 ,最适海拔高度范围依次为 1780～ 2 2 12、170 5～ 195 6、10 4 2～ 16 2 5、82 3～ 1184m ;最适温度范围分别为 - 4 .75～ - 2 .4 0℃、- 3.4 2～ - 2 .0 7℃、- 1.4 9～ 1.39℃、0 .71～ 2 .37℃ ;最适降水范围分别为 10 34～ 1110、10 14～ 10 6 0、883～ 10 17、82 4～ 92 5mm ;长白山自然保护区的森林景观主要分布在平、缓坡地上 ,并与坡向关系密切 ,苔原在各个坡向上均有分布 ,且在各个坡向上分布面积的变化不大 ;岳桦、云冷杉林、阔叶红松林、山杨白桦林主要呈现北、西北向分布 ,其次为东北、西向分布 ;落叶松林主要为东北向分布 ,其次为东和北向分布 ;疏林主要为西向分布 ,其次为西南、西北和南向分布 ;风倒区主要为西、西南、西北向分布 .

黄土塬区3种豆科牧草对土壤水分的消耗利用研究

在田间完全旱作条件下采用3个密度和2种播种方式观察了3种多年生豆科牧草生长第2年对土壤水分的消耗利用情况。结果表明:苜蓿主要耗水深度在2~3 m,最深可达5 m,其中、高密度处理3 m以上土壤水分含量都在稳定田间持水量之下,已经开始形成土壤下伏干层;沙打旺耗水深度在0~2 m,最低含水量(11.61%)处于80~100 cm,在雨季可以恢复到稳定田间持水量之上;达乌里胡枝子主要耗水深度在1 m以上,最低含水量也在稳定田间持水量之上。单播沙打旺、苜蓿和达乌里胡枝子全生长期内对土壤水分的消耗分别为249.9、180.2和136.6 mm,水分利用效率分别是29.39、26.04和8.91 kg.mm-1.hm-2。混播、加大播种密度都会增加3种牧草土壤水分消耗,降低土壤储水量,提高干草产量和水分利用效率,但影响程度因牧草种类、播种方式以及不同的生长时段而异。

五氯酚的微生物降解与光解

五氯酚的微生物降解与光解张春桂许华夏姜晴楠王江炜１）（中国科学院沈阳应用生态研究所，１１００１５）ＭｉｃｒｏｂｉａｌＤｅｇｒａｄａｔｉｏｎａｎｄＰｈｏｔｏｌｙｓｉｓｏｆＰｅｎｔａｃｈｌｏｒｏｐｈｅｎｏｌ．ＺｈａｎｇＣｈｕｎｇｕｉ，ＸｕＨｕａｘｉａ，Ｊ...

White organic electroluminescence based on a new boron complex

A bright blue boron complex BPh2(pybm) containing 2-(2-pyridyl)benzimidazole ligand was designed and synthesized by using N, N-bidentate ligand instead of N, O-bidentate one such as 8-quinolinol. For three-layer LED devices with the configuration of ITO/NPB/BPh2(pybm)/Alq(3)/LiF/Al, the white light emission covering the whole visible region from 400 to 750 nm with the maximum brightness of 110 cd/m(2) and the luminous efficiency of 0.8 cd/A was observed.