Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Green tea extract and its major constituent, epigallocatechin-3-gallate, induce epithelial beta-defensin secretion and prevent beta-defensin degradation by Porphyromonas gingivalis

Background and Objective: Antimicrobial peptides, such as beta-defensins, secreted by gingival epithelial cells, are thought to play a major role in preventing periodontal diseases. In the present study, we investigated the ability of green tea polyphenols to induce human beta-defensin (hBD) secretion in gingival epithelial cells and to protect hBDs from proteolytic degradation by Porphyromonas gingivalis.Material and Methods: Gingival epithelial cells were treated with various amounts (25-200 mu g/mL) of green tea extract or epigallocatechin-3-gallate (EGCG). The secretion of hBD1 and hBD2 was measured using ELISAs, and gene expression was quantified by real-time PCR. The treatments were also carried out in the presence of specific kinase inhibitors to identify the signaling pathways involved in hBD secretion. The ability of green tea extract and EGCG to prevent hBD degradation by proteases of P. gingivalis present in a bacterial culture supernatant was evaluated by ELISA.Results: The secretion of hBD1 and hBD2 was up-regulated, in a dose-dependent manner, following the stimulation of gingival epithelial cells with a green tea extract or EGCG. Expression of the hBD gene in gingival epithelial cells treated with green tea polyphenols was also increased. EGCG-induced secretion of hBD1 and hBD2 appeared to involve extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase. Lastly, green tea extract and EGCG prevented the degradation of recombinant hBD1 and hBD2 by a culture supernatant of P. gingivalis.Conclusion: Green tea extract and EGCG, through their ability to induce hBD secretion by epithelial cells and to protect hBDs from proteolytic degradation by P. gingivalis, have the potential to strengthen the epithelial antimicrobial barrier. Future clinical studies will indicate whether these polyphenols represent a valuable therapeutic agent for treating/preventing periodontal diseases.

Characterization and Quantification of the Compounds of the Ethanolic Extract from Caesalpinia ferrea Stem Bark and Evaluation of Their Mutagenic Activity

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção de cerveja, utilizando méis de diferentes origens botânicas como adjuntos

This study aimed to produce beer, using different botanical origins of honeys (orange, eucalyptus and wild), as malt adjuncts, and their Physical-chemical and sensorial characterizations. The production was made with malt, water, hops and honey. All musts were adjusted to 12 Brix, and the concentration of honey in the formulation was 45% (based on the extract), except for the control (0%). The physical and chemical analysis were performed on malt (extract content), honey (pH, free acidity, lactonic acidity, total acidity, color, turbidity, extract content, moisture content, reducing sugar, total reducing sugar and sucrose) in wort (content extract, pH, color, turbidity, fermentability ,bitterness and total acidity) and beer apparent extract, apparent fermentability, real extract, real fermentability, alcohol content, pH, total acidity, total foam, foam density , bitterness, carbon dioxide, color and turbidity). Sensory analysis was performed by nine-points hedonic scale testing. The attributes evaluated were appearance, aroma, flavor and overall. The results were submitted to ANOVA and means compared by Tukey testing at a 5% of probability. Beer with honey as adjuncts’ had high fermentability and low content of fat, compared to the pure malt beer. The addition of honey as an adjunct did not affect the majority of the physical-chemical parameters, except for turbidity, whereas beer with honey showed the highest value for this feature, in addition, it has also presented their biterness differences the values for beer with honey were lower. The different types of honeys did not affect the acceptability of beer, however, the beer with honey showed greater acceptance between beer with malt and honey

Influência do porta-enxerto, da configuração de extração e do período de maturação no teor de limonina e na aceitação sensorial do suco de laranja Pêra-Rio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Monitoramento do perfil microbiológico de dentes com infecção endodôntica primária durante terapia com diferentes medicações intracanal

Pós-graduação em Odontologia Restauradora - ICT

Antinociceptive, anti-inflammatory and gastroprotective effects of a hydroalcoholic extract from the leaves of Eugenia punicifolia (Kunth) DC. in rodents

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Casearia sylvestris Swartz Extract Release Using Natural Rubber Latex Biomembranes as Carrier

The Natural Rubber Latex (NRL) from Hevea brasiliensis has shown promise in biomedical applications due to its low cost, easy handling, mechanical properties and biocompatibility, being used for bone regeneration and wound healing due to its natural stimulus to angiogenesis. The aim of this work was to incorporate Casearia sylvestris Sw. extract in NRL biomembranes and study its release behavior. The complex membrane-extract has as object of study a new approach of using C. sylvestris extract in the treatment of wounds, for possessing antiseptic activity, anti-inflammatory and analgesic properties. The C. sylvestris species (Salicaceae), popularly known as "guaçatonga", presents great distribution and is used in folk medicine as antiulcer, wound healing, anti-snake venom, properties which have been proven and related to clerodane diterpenes (casearins A-X). The release rate of C. sylvestris compounds from extract-membrane complex was monitored and analyzed using the method of optical spectroscopy (UV-VIS). The release varied with temperature ranging from 14 to 33 days, releasing more than 90%, with an interesting and promising biomedical application, such as wound healing and burns.

Configurando um AVO (Ambiente Virtual Anise de Abreu Gonçalves D'Orange Ferreira de Orientação): instrumentação de um professor em proposta de incentivo ao uso de TIC e AVA no nível de pós-graduação

In this paper, I will present a proposal for teachers’instrumentation in ICT by the configuration of a virtual learningenvironment (MOODLE) for advising Linguistics graduate students’projects on teacher education and language teaching mediated by digitaltechnologies. The context of the proposal is teaching as work,occasioned by an announcement from the IHE to encourage the use ofICT. Following the theoretical assumptions adopted by the ALTERresearch group, and confronted with the goals set in the text of theannouncement, the instrumentation involved the reorganization ofcontents provided by a graduate course in the research process steps:planning, implementation and dissemination, and of the artifactsaffordances: information, communication and production.

Natural latex films as carrier for casearia sylvestris swartz extract associated with ciprofloxacin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of allelopathic potential of leaf extract of kielmeyera coriacea on lactuca sativa l

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Phytotoxicity of Solanum aculeatissimum Jacq: leaves extract

The objective of this study was to evaluate the phytotoxicity of Solanum aculeatissimum Jacq. leaves ethanolic extract in seeds germination, development and fixation of Lactuca sativa seedlings. The same study also aimed to assess the mitotic index of lettuce roots meristematic cells, quantification of phenols and total flavonoids and triage by mean of phytochemical testing of the main secondary metabolites classes. Bioassays of germination, development of root and hypocotyl were carried out in Petri dishes using achenes of Lactuca sativa L. cv. 'Grand Rapids' (lettuce). Concomitantly, were evaluated the physico-chemical characteristics (pH, osmotic potential and electrical conductivity), mitotic index, quantification of total phenols and flavonoids and determination of phytochemical profile of the treatments extract. The results obtained in the bioassays demonstrate that the ethanol extract of S. aculeatissimuma presents phytotoxic potential in the development of lettuce seedlings, given that the concentration of 20 mg/ml showed greater inhibition (41% of germination). The extract contains significant amounts of antioxidants, total flavonoid and phenols, where the concentration 1000µg/mL showed higher values (86.50%). Furthermore, it was possible to observe the presence of compounds with allelopathic activity in the phytochemical screening test as coumarins, tannins, terpenes, flavonoids and alkaloids. Given the above it is clear that the ethanolic extract of S. aculeatissimum presents allelopathic substances with phytotoxic activity that can affect the germination and development of other plant species in their natural environment.

In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)