Two-year follow-op of cognitive functions in Schizophrenia spectrum disorders of adolescent patients treated with electroconvulsive therapy

Objective: The aim of the current study was to investigate the long-term cognitive effects of electroconvulsive therapy (ECT) in a sample of adolescent patients in whom schizophrenia spectrum disorders were diagnosed. Methods: The sample was composed of nine adolescent subjects in whom schizophrenia or schizoaffective disorder was diagnosed according to DSM-IV-TR criteria on whom ECT was conducted (ECT group) and nine adolescent subjects matched by age, socioeconomic status, and diagnostic and Positive and Negative Syndrome Scale (PANSS) total score at baseline on whom ECT was not conducted (NECT group). Clinical and neuropsychological assessments were carried out at baseline before ECT treatment and at 2-year follow-up. Results: Significant differences were found between groups in the number of unsuccessful medication trials. No statistically significant differences were found between the ECT group and theNECT group in either severity as assessed by the PANSS, or in any cognitive variables at baseline.At follow-up, both groups showed significant improvement in clinical variables (subscales of positive, general, and total scores of PANSS and Clinical Global Impressions-Improvement). In the cognitive assessment at follow-up, significant improvement was found in both groups in the semantic category of verbal fluency task and digits forward. However, no significant differences were found between groups in any clinical or cognitive variable at follow-up. Repeated measures analysis found no significant interaction of time · group in any clinical or neuropsychological measures. Conclusions: The current study showed no significant differences in change over time in clinical or neuropsychological variables between the ECT group and the NECT group at 2-year follow-up. Thus, ECT did not show any negative influence on long-term neuropsychological variables in our sample.

Gibberellic acid signaling is required for ambient temperature-mediated induction of flowering in Arabidopsis thaliana.

Distinct molecular mechanisms integrate changes in ambient temperature into the genetic pathways that govern flowering time in Arabidopsis thaliana. Temperature-dependent eviction of the histone variant H2A.Z from nucleosomes has been suggested to facilitate the expression of FT by PIF4 at elevated ambient temperatures. Here we show that, in addition to PIF4, PIF3 and PIF5, but not PIF1 and PIF6, can promote flowering when expressed specifically in phloem companion cells (PCC), where they can induce FT and its close paralog, TSF. However, despite their strong potential to promote flowering, genetic analyses suggest that the PIF genes seem to have only a minor role in adjusting flowering in response to photoperiod or high ambient temperature. In addition, loss of PIF function only partially suppressed the early flowering phenotype and FT expression of the arp6 mutant, which is defective in H2A.Z deposition. In contrast, the chemical inhibition of gibberellic acid (GA) biosynthesis resulted in a strong attenuation of early flowering and FT expression in arp6. Furthermore, GA was able to induce flowering at low temperature (15°C) independently of FT, TSF, and the PIF genes, probably directly at the shoot apical meristem. Together, our results suggest that the timing of the floral transition in response to ambient temperature is more complex than previously thought and that GA signaling might play a crucial role in this process.

AMP-activated protein kinase plays an important evolutionary conserved role in the regulation of glucose metabolism in fish skeletal muscle cells

AMPK, a master metabolic switch, mediates the observed increase of glucose uptake in locomotory muscle of mammals during exercise. AMPK is activated by changes in the intracellular AMP:ATP ratio when ATP consumption is stimulated by contractile activity but also by AICAR and metformin, compounds that increase glucose transport in mammalian muscle cells. However, the possible role of AMPK in the regulation of glucose metabolism in skeletal muscle has not been investigated in other vertebrates, including fish. In this study, we investigated the effects of AMPK activators on glucose uptake, AMPK activity, cell surface levels of trout GLUT4 and expression of GLUT1 and GLUT4 as well as the expression of enzymes regulating glucose disposal and PGC1α in trout myotubes derived from a primary muscle cell culture. We show that AICAR and metformin significantly stimulated glucose uptake (1.6 and 1.3 fold, respectively) and that Compound C completely abrogated the stimulatory effects of the AMPK activators on glucose uptake. The combination of insulin and AMPK activators did not result in additive nor synergistic effects on glucose uptake. Moreover, exposure of trout myotubes to AICAR and metformin resulted in an increase in AMPK activity (3.8 and 3 fold, respectively). We also provide evidence suggesting that stimulation of glucose uptake by AMPK activators in trout myotubes may take place, at least in part, by increasing the cell surface and mRNA levels of trout GLUT4. Finally, AICAR increased the mRNA levels of genes involved in glucose disposal (hexokinase, 6-phosphofructokinase, pyruvate kinase and citrate synthase) and mitochondrial biogenesis (PGC-1α) and did not affect glycogen content or glycogen synthase mRNA levels in trout myotubes. Therefore, we provide evidence, for the first time in non-mammalian vertebrates, suggesting a potentially important role of AMPK in stimulating glucose uptake and utilization in the skeletal muscle of fish.

A single-blind, randomised controlled trial on the effects of lithium and quetiapine monotherapy on the trajectory of cognitive functioning in first episode mania: A 12-month follow-up study.

BACKGROUND: Cognitive deficits have been reported during the early stages of bipolar disorder; however, the role of medication on such deficits remains unclear. The aim of this study was to compare the effects of lithium and quetiapine monotherapy on cognitive performance in people following first episode mania. METHODS: The design was a single-blind, randomised controlled trial on a cohort of 61 participants following first episode mania. Participants received either lithium or quetiapine monotherapy as maintenance treatment over a 12-month follow-up period. The groups were compared on performance outcomes using an extensive cognitive assessment battery conducted at baseline, month 3 and month 12 follow-up time-points. RESULTS: There was a significant interaction between group and time in phonemic fluency at the 3-month and 12-month endpoints, reflecting greater improvements in performance in lithium-treated participants relative to quetiapine-treated participants. After controlling for multiple comparisons, there were no other significant interactions between group and time for other measures of cognition. CONCLUSION: Although the effects of lithium and quetiapine treatment were similar for most cognitive domains, the findings imply that early initiation of lithium treatment may benefit the trajectory of cognition, specifically verbal fluency in young people with bipolar disorder. Given that cognition is a major symptomatic domain of bipolar disorder and has substantive effects on general functioning, the ability to influence the trajectory of cognitive change is of considerable clinical importance.

Evidence for hypothalamic ketone body sensing: impact on food intake and peripheral metabolic responses in mice.

Monocarboxylates have been implicated in the control of energy homeostasis. Among them, the putative role of ketone bodies produced notably during high-fat diet (HFD) has not been thoroughly explored. In this study, we aimed to determine the impact of a specific rise in cerebral ketone bodies on food intake and energy homeostasis regulation. A carotid infusion of ketone bodies was performed on mice to stimulate sensitive brain areas for 6 or 12 h. At each time point, food intake and different markers of energy homeostasis were analyzed to reveal the consequences of cerebral increase in ketone body level detection. First, an increase in food intake appeared over a 12-h period of brain ketone body perfusion. This stimulated food intake was associated with an increased expression of the hypothalamic neuropeptides NPY and AgRP as well as phosphorylated AMPK and is due to ketone bodies sensed by the brain, as blood ketone body levels did not change at that time. In parallel, gluconeogenesis and insulin sensitivity were transiently altered. Indeed, a dysregulation of glucose production and insulin secretion was observed after 6 h of ketone body perfusion, which reversed to normal at 12 h of perfusion. Altogether, these results suggest that an increase in brain ketone body concentration leads to hyperphagia and a transient perturbation of peripheral metabolic homeostasis.

Metodología de la investigación y cine comercial: claves de una experiencia docente

Introducción: El cine se ha configurado, ya desde sus inicios, como una de las recreaciones humanas más extraordinarias que existen desde la perspectiva de la comunicación. Objetivo: El objetivo de este texto es presentar una experiencia docente en la que se empleó cine comercial (CC) en el desarrollo de la asignatura optativa"Investigación en salud: métodos y técnicas", que se imparte en la Escuela de Enfermería de la Universitat de Barcelona. Desarrollo: Los contenidos de esta asignatura son los habituales en los cursos de investigación, y lo más interesante fue el empleo del CC, que se convirtió en el material (objeto) de estudio. En el transcurso de la asignatura, el alumno debía realizar una serie de actividades: revisión bibliográfica, preparación de un cuestionario, selección y visualización de una película de la que debía elaborar la correspondiente ficha técnica y un informe sobre los aspectos referidos a la enfermedad, el paciente, los profesionales y los valores, sentimientos y emociones asociados al problema de salud. Conclusiones: La experiencia puso de manifiesto la importancia de la observación atenta de las escenas para captar los mensajes no verbales relacionados con el problema de salud; la necesidad de adquirir habilidades para el manejo de las bases de datos bibliográfi cas (Medline, CINAHL, etc.), y la conveniencia de una mayor formación en el lenguaje cinematográfico para un mejor aprovechamiento didáctico del CC.

¿Tenemos imágenes literarias de cine?

El cine bebe de la literatura y la tiene en cuenta para desarrollar su versión de unos relatos que antes fueron escritos en forma de novela, poema, obra teatral o cuento. Los personajes literarios se materializan por medio de unos avatares1 humanos o por los que un artista crea en forma de dibujos animados. Se han visto y se ven en las pantallas multitud de versiones de relatos populares o modernos que espectadores de todas las edades reciben expectantes.

'NEDS' ('Non-Educated Delinquent')

Su título, Neds, muestra el hundimiento en el fango de la violencia de John McGill, en un arrabal del Glasgow, capital de Escocia, de los años setenta. Ned es un término aplicado a ciertas personas en Escocia y responde a las siglas de «no educados y delincuentes». La visión estereotipada de un ned es un adolescente varón (aunque este término no excluye a las mujeres), blanco, que lleva ropa de marca, joyas de oro y participa en actos vandálicos vinculados con la pequeña delincuencia; los neds tienen un comportamiento grosero, generan constantes peleas, abusan del consumo de alcohol y del tabaco, etc. En resumen, se caracterizan por su comportamiento antisocial.

Metodología de la investigación y cine comercial: claves de una experiencia docente

Introducción: El cine se ha configurado, ya desde sus inicios, como una de las recreaciones humanas más extraordinarias que existen desde la perspectiva de la comunicación. Objetivo: El objetivo de este texto es presentar una experiencia docente en la que se empleó cine comercial (CC) en el desarrollo de la asignatura optativa"Investigación en salud: métodos y técnicas", que se imparte en la Escuela de Enfermería de la Universitat de Barcelona. Desarrollo: Los contenidos de esta asignatura son los habituales en los cursos de investigación, y lo más interesante fue el empleo del CC, que se convirtió en el material (objeto) de estudio. En el transcurso de la asignatura, el alumno debía realizar una serie de actividades: revisión bibliográfica, preparación de un cuestionario, selección y visualización de una película de la que debía elaborar la correspondiente ficha técnica y un informe sobre los aspectos referidos a la enfermedad, el paciente, los profesionales y los valores, sentimientos y emociones asociados al problema de salud. Conclusiones: La experiencia puso de manifiesto la importancia de la observación atenta de las escenas para captar los mensajes no verbales relacionados con el problema de salud; la necesidad de adquirir habilidades para el manejo de las bases de datos bibliográfi cas (Medline, CINAHL, etc.), y la conveniencia de una mayor formación en el lenguaje cinematográfico para un mejor aprovechamiento didáctico del CC.

XX Setmana de Cinema Formatiu. La Música i el Cinema Formatiu

Seguint la trajectòria iniciada l’any 1995 amb la I Setmana de Cinema Formatiu, enaquesta ocasió es posaran en comú les reflexions al voltant dels aspectes relacionatsamb la Música i el Cinema Formatiu.Aquest novembre tornem amb la XX Setmana del Cinema Formatiu, una activitatconsolidada del Campus Mundet i en la que acostumen a participar any rera any alvoltant d’uns mil estudiants i una trentena de professors i professores.La música ens uneix, ens emociona, ens tranquilitza, ens anima, ens entristeix,... ensacompanya al llarg de tota la nostra vida. Hi ha cultures sense escriptura, però notrobem cultures sense música. La música està present en les nostres vides i també en elcinema. La música il·luminaqualsevol celebració de la nostra vida i es troba en totes lescultures al llarg de la història.És per aquest fet que aquest curs que celebrem la XX Setmana de Cinema Formatiu enel marc del Seminari de Cinema Formatiu dins el Grup Consolidat de Recerca GIAD1389-SGR.2014, que ho volem celebrar amb música i volem que la música sigui elnostre eix central de treball, celebració i trobada...

Chronic postsurgical pain in Europe: An observational study.

BACKGROUND: Chronic postsurgical pain (CPSP) is an important clinical problem. Prospective studies of the incidence, characteristics and risk factors of CPSP are needed. OBJECTIVES: The objective of this study is to evaluate the incidence and risk factors of CPSP. DESIGN: A multicentre, prospective, observational trial. SETTING: Twenty-one hospitals in 11 European countries. PATIENTS: Three thousand one hundred and twenty patients undergoing surgery and enrolled in the European registry PAIN OUT. MAIN OUTCOME MEASURES: Pain-related outcome was evaluated on the first postoperative day (D1) using a standardised pain outcome questionnaire. Review at 6 and 12 months via e-mail or telephonic interview used the Brief Pain Inventory (BPI) and the DN4 (Douleur Neuropathique four questions). Primary endpoint was the incidence of moderate to severe CPSP (numeric rating scale, NRS ≥3/10) at 12 months. RESULTS: For 1044 and 889 patients, complete data were available at 6 and 12 months. At 12 months, the incidence of moderate to severe CPSP was 11.8% (95% CI 9.7 to 13.9) and of severe pain (NRS ≥6) 2.2% (95% CI 1.2 to 3.3). Signs of neuropathic pain were recorded in 35.4% (95% CI 23.9 to 48.3) and 57.1% (95% CI 30.7 to 83.4) of patients with moderate and severe CPSP, respectively. Functional impairment (BPI) at 6 and 12 months increased with the severity of CPSP (P < 0.01) and presence of neuropathic characteristics (P < 0.001). Multivariate analysis identified orthopaedic surgery, preoperative chronic pain and percentage of time in severe pain on D1 as risk factors. A 10% increase in percentage of time in severe pain was associated with a 30% increase of CPSP incidence at 12 months. CONCLUSION: The collection of data on CPSP was feasible within the European registry PAIN OUT. The incidence of moderate to severe CPSP at 12 months was 11.8%. Functional impairment was associated with CPSP severity and neuropathic characteristics. Risk factors for CPSP in the present study were chronic preoperative pain, orthopaedic surgery and percentage of time in severe pain on D1. TRIAL REGISTRATION: Clinicaltrials.gov identifier: NCT01467102.

Detectings low introgression of invasive alleles in an extensively restocked game bird

Interbreeding of two species in the wild implies introgression of alleles from one species into the other only when admixed individuals survive and successfully backcross with the parental species. Consequently, estimating the proportion of first generation hybrids in a population may not inform about the evolutionary impact of hybridization. Samples obtained over a long time span may offer a more accurate view of the spreading of introgressed alleles in a species" gene pool. Common quail (Coturnix coturnix) populations in Europe have been restocked extensively with farm quails of hybrid origin (crosses with Japanese quails, C. japonica). We genetically monitored a common quail population over 15 years to investigate whether genetic introgression is occurring and used simulations to investigate our power to detect it. Our results revealed that some introgression has occurred, but we did not observe a significant increase over time in the proportion of admixed individuals. However, simulations showed that the degree of admixture may be larger than anticipated due to the limited power of analyses over a short time span, and that observed data was compatible with a low rate of introgression, probably resulting from reduced fitness of admixed individuals. Simulations predicted this could result in extensive admixture in the near future.

Tasolohkolinjan läpäisyaikojen tarkastelu ja kehittäminen Lean management periaatteita soveltaen

Diplomityön tavoitteena oli Aker Yardsin Turun telakan tasolohkolinjan läpäisyajan parantaminen. Työssä selvitettiin lohkotehtaan nykyinen läpimenoaika ja pyrittiin parantamaan sitä Lean management periaatteita soveltaen. Työssä suoritettiin perusteellinen työntutkimus yhdessä työnjohdon kanssa, jossa selvitettiin eri työvaiheiden läpimenoajat ja työtunnit. Tuloksista löydettiin kolme selvää kehityskohdetta, joilla saadaan nykyistä läpimenoaikaa ja alihankintakustannuksia pienemmäksi. Nämä kehityskohteet olivat jäykkääjien asennuksen ja hitsauksen parantaminen, myynnin jälkeisten töiden minimointi sekä nostotukien asennuksen tehostaminen. Lisäksi tutkimustyön ohella havaittiin neljäs kehityskohde, osavalmistuksen ajoittaminen oikeaan aikaan. Tämä ei tullut ilmi tutkimustuloksista vaan ihmisiä haastattelemalla. Kehityskohtien tunnistamisen perusteella luotiin investointiehdotus jäykkääjien toiminnan tehostamiseen, sekä uusia toimintatapoja myynnin jälkeisten töiden minimoimiseksi ja nostotukien asennuksen tehostamiseksi. Osavalmistuksen ajoittamisella oikeaan aikaan, pyritään tehostamaan toimintaa lohkonkoontipaikalla.

Individual differences in control of language interference in late bilinguals are mainly related to general executive abilities

Background: Recent research based on comparisons between bilinguals and monolinguals postulates that bilingualism enhances cognitive control functions, because the parallel activation of languages necessitates control of interference. In a novel approach we investigated two groups of bilinguals, distinguished by their susceptibility to cross-language interference, asking whether bilinguals with strong language control abilities ('non-switchers") have an advantage in executive functions (inhibition of irrelevant information, problem solving, planning efficiency, generative fluency and self-monitoring) compared to those bilinguals showing weaker language control abilities ('switchers"). Methods: 29 late bilinguals (21 women) were evaluated using various cognitive control neuropsychological tests [e.g., Tower of Hanoi, Ruff Figural Fluency Task, Divided Attention, Go/noGo] tapping executive functions as well as four subtests of the Wechsler Adult Intelligence Scale. The analysis involved t-tests (two independent samples). Non-switchers (n = 16) were distinguished from switchers (n = 13) by their performance observed in a bilingual picture-naming task. Results: The non-switcher group demonstrated a better performance on the Tower of Hanoi and Ruff Figural Fluency task, faster reaction time in a Go/noGo and Divided Attention task, and produced significantly fewer errors in the Tower of Hanoi, Go/noGo, and Divided Attention tasks when compared to the switchers. Non-switchers performed significantly better on two verbal subtests of the Wechsler Adult Intelligence Scale (Information and Similarity), but not on the Performance subtests (Picture Completion, Block Design). Conclusions: The present results suggest that bilinguals with stronger language control have indeed a cognitive advantage in the administered tests involving executive functions, in particular inhibition, self-monitoring, problem solving, and generative fluency, and in two of the intelligence tests. What remains unclear is the direction of the relationship between executive functions and language control abilities.

Public and Private Production in a Mixed Delivery System: Regulation, Competition and Costs

Academics and policy makers are increasingly shifting the debate concerning the best form of public service provision beyond the traditional dilemma between pure public and pure private delivery modes, because, among other reasons, there is a growing body of evidence that casts doubt on the existence of systematic cost savings from privatization, while any competition seems to be eroded over time. In this paper we compare the relative merits of public and private delivery within a mixed delivery system. We study the role played by ownership, transaction costs, and competition on local public service delivery within the same jurisdiction. Using a stochastic cost frontier, we analyze the public-private urban bus system in the Barcelona Metropolitan Area. Our results suggest that private firms tendering the service have higher delivery costs than those incurred by the public firm, especially when transaction costs are taken into account. Tenders, therefore, do not help to reduce delivery costs. Our results suggest that under a mixed delivery scheme, which permits the co-existence of public and private production, the metropolitan government and the regulator can use private delivery to contain costs in the public firm and, at the same time, benefit from the greater flexibility of private firms for dealing with events not provided for under contract.