De um falsário a outro, de patranhas viageiras a legados críveis (século XV)

In the 15th and early 16th centuries, when traveling eastward and westward no longer proved extraordinary, travel writings, such as those of Marco Polo or Jean de Mandeville, were printed and reprinted and have been in the world of exchanges and acquisitions both in Portugal and in other parts of Europe. However, although they have played a key role in defining foreign worlds for Europe, reflecting the aspirations of their time and providing news about the universe to be discovered, these reports do not always necessarily tell of trips that were actually taken. Several of them, on the contrary, do no more than draw together, for contemporary readers, passages of interest taken from other writings; passages which, based on their regularity and frequency, would allow for a narrative staged as travel to be taken as truth for contemporaries and immediate successors. In the Iberian Peninsula of the late 15th century, an account written by an author about whom nothing is known, Gomez de Santisteban, who defines himself as a companion of Prince Pedro on a supposed trip to the Holy Land, was among those reports integrated into the description and the perception of the land being discovered. The driving question of this paper is, therefore, how Santisteban, though he wrote memories of trips that he did not take, achieved credibility like those travelers whose trips have been recognized as authentic.

Purification of coagulation factor VIII using chromatographic methods. Direct chromatography of plasma in anion exchange resins

Coagulation factor VIII (FVIII) concentrates are used in the treatment of patients with Hemophilia A. Human FVIII was purified directly from plasma using anion exchange chromatography followed by gel filtration. Three Q-Sepharose resins were tested, resulting in 40% recovery of FVIII activity using Q-Sepharose XL resin, about 80% using Q-Sepharose Fast Flow and 70% using the Q-Sepharose Big Beads. The vitamin K-dependent coagulation factors co-eluted with FVIII from the anion exchange columns. In the second step of purification, when Sepharose 6FF was used, 70% of FVIII activity was recovered free from vitamin K-dependent factors.

Production of human factor VIII-FL in 293T cells using the bicistronic MGMT(P140K)-retroviral vector

Hemophilia A is the most common X-linked bleeding disorder; it is caused by deficiency of coagulation factor VIII (FVIII). Replacement therapy with rFVIII produced from human cell line is a major goal for treating hemophilia patients. We prepared a full-length recombinant FVIII (FVIII-FL), using the pMFG-P140K retroviral vector. The IRES DNA fragment was cloned upstream to the P140K gene, providing a 9.34-kb bicistronic vector. FVIII-FL cDNA was then cloned upstream to IRES, resulting in a 16.6-kb construct. In parallel, an eGFP control vector was generated, resulting in a 10.1-kb construct. The 293T cells were transfected with these constructs, generating the 293T-FVIII-FL/P140K and 293T-eGFP/P140K cell lines. In 293T-FVIII-FL/P140K cells, FVIII and P140K mRNAs levels were 4,410 (+/- 931.7)- and 295,400 (+/- 75,769)-fold higher than in virgin cells. In 293T-eGFP/P140K cells, the eGFP and P140K mRNAs levels were 1,501,000 (+/- 493,700)- and 308,000 (+/- 139,300)-fold higher than in virgin cells. The amount of FVIII-FL was 0.2 IU/mL and 45 ng/mL FVIII cells or 4.4 IU/mu g protein. These data demonstrate the efficacy of the bicistronic retroviral vector expressing FVIII-FL and MGMT(P140K), showing that it could be used for producing the FVIII-FL protein in a human cell line.

SK-HEP cells and lentiviral vector for production of human recombinant factor VIII

Hemophilia A is caused by a deficiency in coagulation factor VIII. Recombinant factor VIII can be used as an alternative although it is unavailable for most patients. Here, we describe the production of a human recombinant B-domain-deleted FVIII (rBDDFVIII) by the human cell line SK-HEP-1, modified by a lentiviral vector rBDDFVIII was produced by recombinant SK-HEP cells (rSK-HEP) at 1.5-2.1 IU/10(6) in 24 h. The recombinant factor had increased in vitro stability when compared to commercial pdFVIII. The functionality of rBDDFVIII was shown by its biological activity and by tail-clip challenge in hemophilia A mice. The rSK-HEP cells grew in a scalable system and produced active rBDDFVIII, indicating that this platform production can be optimized to meet the commercial production scale needs.

Abastecimiento y distribución de tabacos en el marco del estanco general español (siglos XVII y XVIII)

[ES]El presente estudio pretende introducir una serie de propuestas cuyo contraste empírico resulta dificultoso en ocasiones por encontrarse relacionado con actividades económicas que ofrecen un escaso rastro documental, como son la gestión de rentas bajo la fórmula de arriendo o la influencia del contrabando sobre el consumo real de un producto como el tabaco. El propósito en este sentido es diverso: profundizar en el conocimiento de las fórmulas y géneros que abastecen el monopolio tabaquero durante el siglo XVII; analizar los cambios introducidos en la gestión de la renta durante el primer tercio del XVIII dirigidos a obtener un mayor control sobre el monopolio tabaquero; y, finalmente, abordar la importancia creciente durante la segunda mitad de la centuria dieciochesca de las adquisiciones de tabaco de humo con respecto al inicial dominio del consumo de tabaco polvo

Curanderos, sanadores y santiguadoras en Lanzarote entre los siglos XVI y XIX

[ES] Curanderos y sanadores nacieron ante la necesidad de hacer frente a los escasos medios que tenía la isla para curar sus enfermedades, la poca confianza que les daban los conquistadores y sobre todo ante la aparición de nuevas epidemias y plagas, que año tras año dejaban los visitantes y transeúntes. Partiendo de la premisa de que la “magia” popular es utilitaria, un medio para lograr un fin.