Faecal indicator organisms in frozen prawn products. Pt. 2. Survival of the organisms during freezing and frozen storage

The rate of survival of different types of faecal indicator organisms like Escherichia coli, enterococci etc. during freezing and frozen storage has been studied. Peeled and deveined prawns inoculated with a mixed culture of the above organisms were subjected to freezing and storage at -10̊F and examined for over four months.

Faecal indicator organisms in frozen prawn products. Pt. 1. Incidence and general distribution

A general survey carried out on several brands of frozen prawn products has shown that along with the standard plate count (SPC), the numbers of pathogenic organisms like Escherichia coli, enterococci and coagulase positive staphylococci have also to be taken into consideration for the evaluation of the quality of these products. No correlation could be established between the total plate count and the number of E. coli, enterococci or staphylococci. Enumeration of enterococci, however, is advocated as a better index of faecal contamination of the products than E. coli.

Some observations on the problem of marine timber destroying organisms of Indian coasts

In India the chief marine timber boring organisms are 2 species of Martesia, 28 species of shipworms, 4 species and a variety of Sphaeroma and 9 species of Limnoria besides bacteria and fungi. The occurrence, abundance and activity of the various species of borers show remarkable variations and fluctuations in the different harbours of India, each harbour or area having its own dominant set of species and an assemblage of less important forms. These species have their own characteristic preferences, life history and seasons of attachment and a scheme evolved for one locality may prove ineffective for another. Through a delicate and complex ecological adjustment the borers occurring in a locality have reached an interrelationship reducing interspecific and intraspecific competition. The seasons of settlement of the dominant borers in the different harbours of India are indicated. The need for a detailed biological enquiry is stressed.

Effect of different media on the determination of the number of micro organisms in frozen prawns (shrimp)

The Indian standards Institution have recently laid down specifications for the grading of frozen shrimp of which the bacterial examination of the product forms a significant part. Work carried out in this regard in our laboratories have shown that various improvements could be made in the composition of the medium to obtain a more true picture of the total bacterial count. Changes in the medium for total bacterial count have, therefore, been suggested and the reasons for the same discussed.

Post-larval production of Macrobrachium rosenbergii using mass-cultured live food organisms

Macrobrachium rosenbergii post-larvae were produced in 1992 and 1993 using Artemia nauplii and cultured zooplankton Brachionus plicatilis (rotifier), Apocyclops dengizicus (copepod) and Moina sp. (cladoceran) supplemented with chopped Tubifex worms. In 1992 (first trial) two experiments were carried out under water temperature range of 24.5 to 28°C and 26.0 to 28.5 °C respectively and corresponding post-larval production was 5.6% and 86.3%. The duration of experiments was 58 and 40 days. During second trial in 1993 water temperature varied between 25.0 to 27.0°C. At the end of 59 days the post-larvae were found to be 44% of the total number of larvae stocked on the first day.

Embankment damaging marine organisms and their control measures using calcium carbide

Five species of crabs and three species of fishes were the main marine organisms found damaging embankments owing to their burrowing nature. Among crabs, Scylla serrata was in the highest percentage, while among fishes, Boleopthalamus dussumieri was recorded in maximum percentage calcium carbide 10-15 per burrow was effective in eradicating the crabs.

Modified Kanerva model. Results for real time word recognition

This paper describes results obtained using the modified Kanerva model to perform word recognition in continuous speech after being trained on the multi-speaker Alvey 'Hotel' speech corpus. Theoretical discoveries have recently enabled us to increase the speed of execution of part of the model by two orders of magnitude over that previously reported by Prager & Fallside. The memory required for the operation of the model has been similarly reduced. The recognition accuracy reaches 95% without syntactic constraints when tested on different data from seven trained speakers. Real time simulation of a model with 9,734 active units is now possible in both training and recognition modes using the Alvey PARSIFAL transputer array. The modified Kanerva model is a static network consisting of a fixed nonlinear mapping (location matching) followed by a single layer of conventional adaptive links. A section of preprocessed speech is transformed by the non-linear mapping to a high dimensional representation. From this intermediate representation a simple linear mapping is able to perform complex pattern discrimination to form the output, indicating the nature of the speech features present in the input window.

The modified Kanerva model for automatic speech recognition

A parallel processing network derived from Kanerva's associative memory theory Kanerva 1984 is shown to be able to train rapidly on connected speech data and recognize further speech data with a label error rate of 0·68%. This modified Kanerva model can be trained substantially faster than other networks with comparable pattern discrimination properties. Kanerva presented his theory of a self-propagating search in 1984, and showed theoretically that large-scale versions of his model would have powerful pattern matching properties. This paper describes how the design for the modified Kanerva model is derived from Kanerva's original theory. Several designs are tested to discover which form may be implemented fastest while still maintaining versatile recognition performance. A method is developed to deal with the time varying nature of the speech signal by recognizing static patterns together with a fixed quantity of contextual information. In order to recognize speech features in different contexts it is necessary for a network to be able to model disjoint pattern classes. This type of modelling cannot be performed by a single layer of links. Network research was once held back by the inability of single-layer networks to solve this sort of problem, and the lack of a training algorithm for multi-layer networks. Rumelhart, Hinton & Williams 1985 provided one solution by demonstrating the "back propagation" training algorithm for multi-layer networks. A second alternative is used in the modified Kanerva model. A non-linear fixed transformation maps the pattern space into a space of higher dimensionality in which the speech features are linearly separable. A single-layer network may then be used to perform the recognition. The advantage of this solution over the other using multi-layer networks lies in the greater power and speed of the single-layer network training algorithm. © 1989.

Breeding biology and monosex male seed production of Genetically Improved Farmed Tilapia (GIFT) strain of Oreochromis niloticus L. in Bangladesh

A study on the breeding biology of the GIFT strain of Nile tilapia, Oreochromis niloticus, was conducted for a period of five months. The sex ratio of the parent individuals was optimized for performance in spawn production, where the best results were obtained with a sex ratio of female to male of 4:1 compared to those of 3:1 and 2:1. The diameter of eggs obtained from the GIFT stock had major and minor axes of 2.19±0.09 and 1.72±0.07 mm, respectively, with no significant differences between the treatments. The average number of eggs produced was 392±22 per female, with fertilization and hatching rates ranging between 94-96% and 85-88%, respectively. No significant variation was observed between the treatments. Breeding frequencies per female in the three treatment groups ranged between 10-40 days and the highest value was obtained at a female to male sex ratio of 4: l. In an other experiment, l7 aMethyltestosterone (MT) was applied orally to the fry at their first feeding stage with treatments- 1, 2, 3 and 4 at the dosage of 100, 80, 60 and 40 mg/kg feed respectively, for the period of 28 days. The mean percentage of males obtained in treatments, 1, 2, 3 and 4 were 98, 97, 95 and 68, respectively. Treatments-1, 2 and 3 did not differ significantly (P>0.05) from each other but treatment 4 showed significant variation (P<0.05) from other treatments. The results showed that MT-100, 80 and 60 mg/kg feed administered for 28 days produced close to cent percent male population of the GIFT strains in aquaria.

Establishment of satellite nucleus of genetically improved farmed tilapia (GIFT strain) in Bangladesh

The aim of this study was to evaluate growth performance of the GIFT (Genetically Improved Farmed Tilapia) strain after one generation of selection for increased body weight at Bangladesh Fisheries Research Institute. Founder stock comprised of 30 families having 300 individuals of the GIFT strain were introduced from Malaysia through \X!orldFish Center in March 2005. The founder stock was reared in 100 m2 hapa for three months and then individually tagged using Passive Integrated Transponder (PIT) at the weight between 30 and 40 g. After tagging, all the fish were communally grown out in pond until harvest. Breeding value for body weight was estimated using SAS and ASREML ranging from 4.17 to 9.70 g for males and 4.24 to 9.36 g for females. The best 40 females and 40 males from the founder stock were then selected to produce progeny of the first generation (F-1). From each family 25 female and 25 male fingerlings were sampled and tagged using PIT. A total of 2,000 tagged fish from 40 families were stocked in a pond (1000 m2) for a continuation of the selection program. In addition, surplus fish after tagging were also reared together with progeny of the founder stock in cistern ecology for growth evaluation. The mean weight of the F-1 generation of GIFT fish was 7.2% greater than that of the founder population (non selected population).

Performance of genetically improved farmed tilapia (GIFT) under mono and mixed cui ture with silver barb (Barbodes gonionotus) in south-west Bangladesh

A participatory on-farm trial was carried out to evaluate the production performance of GIFT (genetically improved farmed tilapia) strain of Oreochmis sp., either alone or with silver barb (Barbodes gonionotus), in six rain-fed freshwater ponds of coastal area. There vvere two treatments; (i) GIFT alone at a stocking density of 24,700/ha (T1) and (ii) 1:1 combination of GIFT and silver barb (T1). Each of the treatments had three replications. A significantly (p

Effect of modified starch on the shelf life of frozen fish Kamaboko with prepared spinach

Fish kamaboko with spinach was prepared by mixing fish kamaboko with spinach prepared with different combinations of ordinary starch (OS) and modified starch (MS), viz., 40:60, 50:50, 60:40, 100%) MS and 100% OS. The products were frozen at -40°C and stored at -20°C, and subjected to biochemical (peroxide value, total titratable acidity, pH and moisture) organoleptic (appearance, taste and colour), microbiological (total plate count) and physical (folding and expressible water) tests at monthly intervals. Among the different combinations tried, it was observed that fish kamaboko with spinach prepared with 50:50 and 60:40 combinations of OS and MS could be stored for six months at-20°C.

Seed production and culture techniques of Genetically Improved Farmed Tilapia (GIFT) in brackishwater environment

Effects of different levels of salinity on survival, growth and gonadal development of Genetically Improved Farmed Tilapia (GIFT) were studied under laboratory conditions in glass aquarium, for a period of ten weeks. The initial individual size of the GIFT was 20.23±4.45 and the salinity levels tested were 0, 5, 10, 15 and 20 ppt. The highest survival of 87.5% was found in 0 ppt and the lowest 60.5% in 20 ppt. Though the survival decreased progressively with increased salinity, there were no significant differences (P>0.05) among 0, 5, and 10 ppt. Similar to what has been observed in survival, the specific growth rate (SGR %/day) also decreased as of 1.30, 1.24, 1.08, 0.90 and 0.71, respectively, with the increased salinity of 0, 5, 10, 15 and 20 ppt. The gonadal development was highest in 0 ppt with a GSI value of 3.75 and lowest of 2.01 in 20 ppt. In the second experiment, gonadal development and seed production performance of GIFT in brackishwater condition were investigated for a period of three months. Each of the three fine meshed hapas of 20 square meters made from nylon net was placed in a freshwater (0 ppt) and in a brackish water (10-15 ppt) pond of the Brackishwater Station (BS). GIFT of 65 g average weight from a single cohort were stocked into three hapas at a rate of 2 per m. The male vs female ratio was 1:3. The development of gonad was faster with the higher gonadosomatic index (GSI %) of 3.85 % in freshwater condition than that of 2.73 % in brackish water. Within three months of the study period, a total of 70,510 and 44,250 GIFT fry were produced respectively, in freshwater and brackishwater conditions. Finally under third experiment, a participatory on-farm trial was carried out to evaluate the production performance of GIFT in monoculture and in polyculture with silver barb in coastal freshwater pond conditions. Nine ponds were selected for three treatment combinations of GIFT monoculture (T1), GIFT and silver barb polyculture (T2), and silver barb monoculture (T3). The ponds have been stocked in April, 05 at a density of 25,000 fry per ha. Fishes were fed with rice bran at the rate of 6% bw per day. In one month culture period, GIFT attained an average weight of 16.27 g in monoculture and 17.23 g in polyculture, against an average stocking weight of 0.37 g. Silver barb reached an average weight of 16.62 g in polyculture with GIFT and 10.01 g in monoculture, against an average stocking weight of 3.79 g.

Effects of black Bengal goat manure on growth and production of Genetically Improved Farmed Tilapia (GIFT) starin [sic], Oreochromis niloticus

In a goat-tilapia integrated farming system, the effect of Black Bengal goat manure on the growth and production of Oreochromis niloticus was studied at the Freshwater Substation, Shantahar, Bogra for 4.5 months. The stocking density used in three treatments were, 200 goats and 15,000 GIFT strain/ha (T1); 300 goats and 15,000 GIFT strain/ha (T2); and only 15,000 GIFT strain/ha (T3). The initial individual total length and weight of stocked tilapia were 7.6 cm and 11.34 g, respectively. Twelve ponds each having an area of 40 square meters were used for this trail [sic]. On one side of each pond goat shed was constructed and the space allocated for each goat was 0.75 m x 1.5 m. Newly weaned black Bengal goats of average weight 8.45 kg was used in the trial. Every morning the goats manure was swiped out in ponds through the fixed opening of bamboo made floor of goat shed. Water depth of the ponds was maintained at 0.75 m. The highest fish production was in treatment 1 (1,750 kg/ha) followed by treatment 2 (1,455 kg/ha) and treatment 3 (621 kg/ha). The difference in individual goat weight was not significant (P>0.05) among treatments 1 and 2.

Experiments with fibre-glass sheathing as a protection against marine wood-boring organisms

Wooden test panels of different species of wood sheathed with fibre-glass reinforced plastic were subjected to immersion tests along with corresponding controls in Cochin Port area and the effect of the protective coating studied.