Methylation of tumor associated genes in tissue and plasma samples from liver disease patients

To investigate whether aberrant hypermethylation in plasma DNA could be used as diagnosis makers for hepatocellular carcinoma (HCC), we performed methylation-specific PCR (MSP) to check the methylation status of five tumor associated genes in 36 cases of

Cathodic protection of aluminium sheathed wooden fishing boats with ternary aluminium anodes

Results of laboratory and field trials on cathodic protection of aluminium sheathing in fishing boats by ternary aluminium anodes are presented. The high negative potential of 1.06 V with respect to saturated calomel electrode, its appreciably low anodic polarization and high current output are favourable factors for using the ternary aluminium anodes. The low rate of consumption of the anode material under service trials attests its economic viability.

Cathodic protection of the hulls of fishing trawlers in India

Nearly 10,000 mechanised fishing trawlers mostly built of wood and about 100 trawlers built of steel besides a few fiberglass reinforced plastic and a couple of ferro-cement boats constitute the modern fishing fleet of India at present. Metallic corrosion in sea water is a very well-known phenomenon in all ships and various other marine structures; the exact financial loss and the material breakdowns have never been fully realized among the trawler owners in India. The Central Institute of Fisheries Technology at Cochin has been studying these problems for some years and has been able to assess the significance of underwater corrosion particularly of the hull below water line in the trawlers and suitable remedial measures have been suggested in this paper.

Optical emission spectroscopy of the plasma during sputter deposition of YBCO films for microwave applications

YBCO thin films are currently used in several HTS-based electronics applications. The performance of devices, which may include microwave passive components (filters, resonators), grain boundary junctions or spintronic multilayer structures, is determined by film quality, which in turn depends on the deposition technology used and growth parameters. We report on results from nonintrusive Optical Emission Spectroscopy of the plasma during YBCO thin film deposition in a high-pressure on-axis sputtering system under different conditions, including small trace gas additions to the sputtering gas. We correlate these results with the compositional and structural changes which affect the DC and microwave properties of YBCO films. Film morphology, composition, structure and in- and out-of-plane orientation were assessed; T, and microwave surface resistance measurements were made using inductive and resonator techniques. Comparison was made with films sputtered in an off-axis 2-opposing magnetron system.

On the development of a mercury-free ternary aluminium anode for cathodic protection

The development of a new mercury-free ternary aluminum anode (CIFTAL) for cathodic protection of marine structures is described. The new anode demonstrated a current efficiency of 83.5% to 85.4% in a current density range of 5.6 to 166.7 mAdmˉ². The current efficiency remained practically stable at 1.4 mAdmˉ² over a test period of 300 days. The service trials of the anode on steel trawlers and aluminum (Indal M 57 S) sheathed wooden boats have shown satisfactory performance in terms of uniform dissolution, current efficiency and driving voltage. In the wake of legislations restricting the use of anodes containing mercury in an endeavor to control the mercury pollution of the near shore aquatic environment, the new anode (CIFTAL) with its stable current output and high current efficiency merits significance in marine cathodic protection.

Integrated cage-cum-pond culture systems with high valued climbing perch (Anabas testudineus) in cages and low-valued carps in open ponds

An on-farm trial was conducted over 150 days to determine appropriate stocking ratio, growth and production of climbing perch (Anabas testudineus) in cages and carps in open water of ponds in eighteen farmers' ponds from Haluaghat Upazila at Mymensingh district of Bangladesh. One or two 1 m super(3) cage was suspended in each of 12 earthen ponds and other 6 ponds served as control without cages. Climbing perch of 2-3 g in size were stocked in cages while fingerlings of silver carp (Hypophthalmicthys molitrix), catla (Catla catla), rohu (Labeo rohita), mirgal (Cirhinus cirrhosus), rajputi (Puntius sarana) and common carp (Cyprinus carpio) were stocked at 1 fish/m super(2) with a species ratio of 5:4:4:4:2:1 in open water of all ponds to give cage to open-pond fish ratios of 1:1 (T sub(1:1)) and 2:1 (T sub(2:1)) and 0:1 (T sub(0:1)) as three treatments with six replicates each. Survival of climbing perch was higher in T sub(1:1) (61.67%) than that of T sub(2:1) (29.5%) and was significantly different (p>0.05) between the treatments. Stocking of small size climbing perch fry increased the mortality rate in cages. The net yields of Thai koi were 0.13±0.01 (t/ha) and 0.10±0.01 (t/ha) in treatments T sub(1:1) and T sub(2:1), respectively and both were significantly different (p>0.05). Survival of-open-pond carps was high, ranging from 50 to 91.67% with significantly lower in T sub(0:1) than that of T sub(1:1) and T sub(2:1) treatment. Net and gross yield of each carp species were significantly higher in the T sub(1:1) and T sub(2:1) treatment than that in T sub(0:1) treatment. Net revenues were positive but low in all treatments. Therefore, bigger size climbing perch with lower stocking ratio (T sub(1:1)) is suitable for integrated cage-pond culture of climbing perch and carps. However, more on-farm trials in different ecosystem with scientific interventions are necessary to develop the technology for further dissemination among the rural farmers.

Socioeconomic effects of intensive cage culture

The paper discusses the socioeconomic effects of intensive aquaculture which is in conflict with other forms of coastal development, such as industrial development, natural fisheries, tourism and recreation, and nature conservation.

The basics of cage and pen culture

The article discusses the basics of cage and pen aquaculture. Factors to consider are: site selection; choice of nets, floats, frames, and anchors; and the choice of culture method.

Cage culture of sea bass in Malaysia

The article discusses the cage culture practice of sea bass (Lates calcarifer) in Malaysia. Problems on feed and seed supply and overcrowding are also discussed. Despite these problems, seabass cage culture still continuously booms.

Technical report on the environmental monitoring of the cage area at the Source of the Nile (SON) Fish Farm for quarter 4: October-December 2011

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm requested for technical assistance of NaFIRRI to undertake regular environment monitoring of the cage site as is mandatory under the NEMA conditions. As the SON is a key collaborator/client of the institute, NAFIRRI agreed to undertake the assignment subject to facilitation by the client. The institute agreed to conduct quarterly surveys of key environmental parameters at the site including selected physical-chemical and biological factors, nutrient status, column depth, water transparency and sedimentation. Samples and field measurements were to be taken at 3 sites: within and/or close to the fish cages (WIC), upstream (USC) and downstream (DSC) of the cages. The first environmental monitoring survey was undertaken in February 2011; the second in May 2011 and the third in September 2011. The surveys cover physical-chemical parameters, nutrient status, invertebrate and fish communities. The present report presents field observations made for the fourth quarter survey undertaken in November 2011 and provides a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and the different aquatic biota at and around the cage site including natural fish communities.