Methylation of tumor associated genes in tissue and plasma samples from liver disease patients

To investigate whether aberrant hypermethylation in plasma DNA could be used as diagnosis makers for hepatocellular carcinoma (HCC), we performed methylation-specific PCR (MSP) to check the methylation status of five tumor associated genes in 36 cases of

We sincerely thank Ms. Fan (Kunming Institute of Zoology) for cell culture and prepare tissue sections. This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M).