First detection of Leishmania infantum DNA within the brain of naturally infected dogs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

2 '-Deoxyguanosine, 2 '-deoxycytidine, and 2 '-deoxyadenosine adducts resulting from the reaction of tetrahydrofuran with DNA bases

A recent study showed that tetrahydrofuran (THF), a widely used solvent, is carcinogenic in experimental animals. Despite its carcinogenic activity, there is a paucity of information regarding cellular toxicity, biomolecular damage, and genotoxicity induced by THF. We describe here the structural characterization of adducts produced by the reaction of oxidized THF with 2'-deoxyguanosine (dGuo-THF 1 and dGuo-THF 2), 2'-deoxyadenosine (dAdo-THF), and 2'-deoxycytidine (dCyd-THF). Adducts were isolated from in vitro reactions by reverse-phase HPLC and fully characterized on the basis of spectroscopic measurements. The stable derivatives obtained by the reduction of adducts with NaBH4 ( the case of dGuo-THF 1, dCyd-THF, and dAdo-THF) and the stable adduct dGuo-THF 2 were used as standards for optimization of chromatographic separations for adduct detection in DNA through HPLC/ESI/MSMS. Using this methodology, we successfully detected the four adducts in calf thymus DNA reacted with oxidized THF. The present study also provides evidence that rat liver microsomal monooxigenases oxidize THF to the reactive electrophilic compounds that are able to damage the DNA molecule, as indicated by a significant increase in adduct dGuo-THF 1 level when NADPH was added to the THF/ microsomes/dGuo incubation mixtures. Our data point to DNA-THF adducts as possible contributing factors to the toxicological effects of THF exposure.

Origin and Evolution of B Chromosomes in the Cichlid Fish Astatotilapia latifasciata Based on Integrated Genomic Analyses

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Single Origin of Sex Chromosomes and Multiple Origins of B Chromosomes in Fish Genus Characidium

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Possible interspecific origin of the B chromosome of Hypsiboas albopunctatus (Spix, 1824) (Anura, Hylidae), revealed by microdissection, chromosome painting, and reverse hybridisation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção de DNA de micobactérias dos complexos M. tuberculosis e M. avium em amostras parafinadas de pacientes transplantados renais

Introduction: Tuberculosis (TB) is a granulomatous disease caused by Mycobacterium tuberculosis. The genus Mycobacteriumhas two different complexes: M. tuberculosis Complex and M. avium Complex. This is a global health epidemic and remains a major global health problem, besides, the clinical severity of TB is significantly higher in transplanted patients. The detection of these mycobacteria complexes in transplanted patients, by molecular methods, is fundamental for quick treatment of patients and can contribute for rapid and accuracy of diagnosis. Objective: To detect mycobacteria DNA of M. tuberculosis and M. avium Complexes in formalin fixed paraffin-embedded samples (FFPE) of two patients groups: non transplanted and transplanted. Materials and Methods: The study includes 40 FFPE biopsies separated in four groups: NTP – presence of epithelioid granuloma and positive ZN, non-transplanted patients – 9 samples; NTN - presence of epithelioid granuloma and negative ZN, non-transplanted patients – 10 samples; TP – positive ZN, transplanted patients – 9 samples; TN – negative ZN, transplanted patients – 7 samples. Sections were cut for DNA extraction. Samples were submitted to PCR for amplification of: a) β-actin, b) IS6110 insertion and c) IS1245 insertion. DNA evaluation was made by spectrophotometry and efficiency and PCR analysis was made by agarose gels under UV light. Results: In all samples processed, 97.1% were positive for human β-actin gene. In22.2% of NTP group were found the IS6110 insertion sequencebut the IS1245 wasn´t. In the NTN group was not found any sequence. In theTP group, 11.1% of the samples were positive for IS6110 and also 11,1% werepositive for IS1245. In the TN group, 14.3% of the samples were positive forIS6110 and for IS1245, 14.3% was also positive. Conclusion: Although factors such as DNA degradation after formalin fixation and paraffin embedding, were possible to detect DNA from the human gene ...

Diversidade gênica no gênero Astyanax (Teleostei : Characidae) através da análise de PCR-RFLP de DNA mitocondrial

O gênero Astyanax é um dos mais abundantes e diversificados da família Characidae (subfamília Tetragonopterinae), com mais de 100 espécies nominais, estando amplamente distribuído na bacia do Alto rio Paraná. Esse gênero é caracterizado pela similaridade quanto à forma do corpo, além da alta variabilidade citogenética intra e interpopulações, sendo comum a ocorrência de espécies crípticas ou complexos de espécies. Devido à escassez de dados sobre genética molecular referentes a esse gênero, e a dificuldade na identificação taxonômica, fazse necessário um estudo utilizando marcadores moleculares que visem desenvolver métodos rápidos e eficientes para caracterização de espécies de Astyanax com base em análises de DNA. Em vista dessa necessidade, o presente trabalho teve como objetivo avaliar a eficiência da técnica de PCR-RFLP do gene mitocondrial Citocromo b na identificação e caracterização da variabilidade genética de cinco espécies do gênero Astyanax que ocorrem na bacia do Alto rio Paraná. O mtDNA das espécies alvo foi totalmente amplificado, num total de cerca de 1140 pares de bases (pb). As análises foram obtidas através dos haplótipos gerados com a digestão por três enzimas de restrição que clivam estes genes, sendo estas ALUI, BAMHI, e HPAII. Foram analisadas 2 populações de Astyanax paranae, A. altiparanae, A. fasciatus, A. bockmanni, e uma população de A. biotae, com amostragens variando entre 5 e 10 indivíduos de cada população. Como grupo externo foram analisadas duas populações de Astyanax ribeirae da bacia hidrográfica do rio Ribeira de Iguape. Ao final do trabalho foi possível a identificação de 4 das 6 espécies analisadas, sendo que as espécies mais divergentes são A. altiparanae e A. ribeirae, as espécies A. paranae + A. bockmanni e A. fasciatus + A. ribeirae são as mais fortemente relacionadas...(Resumo completo, clicar acesso eletrônico abaixo)

Análise da relação filogenética entre Triatoma carcavalloi Jurberg, Rocha; Lent, 1998; Triatoma circummaculata Stal, 1859; Triatoma klugi Carcavallo, Jurberg, Lent; Galvão, 2001 e Triatoma rubrovaria Blanchard, 1843 (Hemiptera, Reduviidae) baseada no sequenciamento de genes do DNA mitocondrial e nuclear

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Oxidative stress and nuclear dna damage in hyperglycemic pregnancies

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)