Accelerating food safety and quality control: The use of antibody engineering techniques in the detec-tion of low-molecular weight food contaminants

The increased awareness and evolved consumer habits have set more demanding standards for the quality and safety control of food products. The production of foodstuffs which fulfill these standards can be hampered by different low-molecular weight contaminants. Such compounds can consist of, for example residues of antibiotics in animal use or mycotoxins. The extremely small size of the compounds has hindered the development of analytical methods suitable for routine use, and the methods currently in use require expensive instrumentation and qualified personnel to operate them. There is a need for new, cost-efficient and simple assay concepts which can be used for field testing and are capable of processing large sample quantities rapidly. Immunoassays have been considered as the golden standard for such rapid on-site screening methods. The introduction of directed antibody engineering and in vitro display technologies has facilitated the development of novel antibody based methods for the detection of low-molecular weight food contaminants. The primary aim of this study was to generate and engineer antibodies against low-molecular weight compounds found in various foodstuffs. The three antigen groups selected as targets of antibody development cause food safety and quality defects in wide range of products: 1) fluoroquinolones: a family of synthetic broad-spectrum antibacterial drugs used to treat wide range of human and animal infections, 2) deoxynivalenol: type B trichothecene mycotoxin, a widely recognized problem for crops and animal feeds globally, and 3) skatole, or 3-methyindole is one of the two compounds responsible for boar taint, found in the meat of monogastric animals. This study describes the generation and engineering of antibodies with versatile binding properties against low-molecular weight food contaminants, and the consecutive development of immunoassays for the detection of the respective compounds.

Developing asset management in district heating and cooling business in a large energy company

The purpose of this Master´s Thesis is to develop asset management and its practices in case company. District heating and cooling systems operated by case company around Finland, Sweden, Poland and the Baltics form an enormous-sized asset base where some parts are starting to reach their end of life-cycles. Large-sized asset renewal actions are under discussion and maintenance spending is increasing. Financially justified decisions in changing business environment are needed. Asset management is one of the most important concepts for production organization which operates with capital-intensive production assets. Organizations profitability is highly dependent on assets´ performance. Such assets, like district heating and cooling systems, should be utilized as efficiently as possible within their life-cycles but also maintained and renewed optimally. In this qualitative thesis, empirical interview study was conducted to describe the current situation on how the assets are managed in the case company and to examine the readiness to implement a new, risk-based solution. Asset management revealed to be a very well-known concept. From proposed risk-based asset management point of view, several key observations were made. It was seen as a suitable solution, but further development will be needed. Based on the need and findings, several key processes and frameworks were created and also tested with a case study. Assets` condition monitoring should be improved, which would have a positive impact on event probability assessment. Risk acceptance is also a thing to be discussed further. When the evaluation becomes fluent in single investment cases, portfolio-level expansion should be considered and started. As a result, thesis proposes a solution how risk-based asset management could be performed practically in a capital-intensive case company in order to optimize the maintenance spending in a long run. Created practical framework is made universal: similar principles can be applied into multiple cases in case company but also in other energy companies. Risk-based asset management`s benefits could be utilized best in portfolio-level optimization where the capital would be invested to the most important objects from total risk point of view. Eventually, such approach would allow case company to optimize capital spending in a situation where funds are not adequate to cover all the mandatory needs and prioritization between the investment alternatives will truly be needed.

Solving large sparse linear systems over the field GF(2)

Nowadays problem of solving sparse linear systems over the field GF(2) remain as a challenge. The popular approach is to improve existing methods such as the block Lanczos method (the Montgomery method) and the Wiedemann-Coppersmith method. Both these methods are considered in the thesis in details: there are their modifications and computational estimation for each process. It demonstrates the most complicated parts of these methods and gives the idea how to improve computations in software point of view. The research provides the implementation of accelerated binary matrix operations computer library which helps to make the progress steps in the Montgomery and in the Wiedemann-Coppersmith methods faster.

A map or chart of the Gulfs of Finland and Livonia with their respective ports and harbours, together with a large Plan of Kroonstad and St. Petersburg, taken from an original drawing lately sent from St. Petersburg

Sold by M. Senex at the Globe over against St. Dunstans Church in Fleetstreet London.

Preparation and Investigation of Bio-coal Mixture Fuels Used in Large Diesel Engines

Coal slurry was of vital interest during the last century due to its potential as an alternative fuel where liquid fuels were necessary. Recently, environmental impacts of the traditional fuels, similarities of bio-coal to that of coal, and huge bio-coal supply has attracted the attention to prepare bio-coal slurries as a new fuel. Rudolf Diesel who invented the diesel engine on 1895 was of the opinion that diesel engines are capable to use different kinds of fuels due to the special design. He tried some kind of vegetable oil to operate on his IC engine. Recently, due to high energy density and more environmentally friendly fuel, researchers believe that bio-coal slurries could act as a new alternative fuel in large diesel engines. Loads of research on different kinds of bio-coal slurry were done by the other researchers worldwide and a lot of progress to boost slurry’s quality were achieved recently. The present study aims to achieve the ideal condition of different factors affecting on the quality of bio-coal slurry. One charcoal sample and two kinds of torrefied wood were used to investigate and compare the reaction of various factors. The results show a great gap between the quality of slurries made of different samples and more researches are necessary to fully understand the impact of the different parameter and improving the quality.

Suorituskyvyn johtamisen ja mittaamisen kehittäminen suuren yrityksen tutkimus- ja tuotekehitystoiminnassa

Tässä tutkimuksessa selvitettiin, miten suorituskyvyn johtamista ja mittaamista käytännössä toteutettiin suuressa tutkimus- ja tuotekehitystoimintaa Suomessa harjoittavassa elintarvikealan yrityksessä. Tutkimuksessa kartoitettiin tärkeimpiä suorituskykyyn vaikuttavia tekijöitä suorituskyvyn johtamisen ja mittaamisen näkökulmasta. Tutkimuksen tavoitteena oli saada kooste suosituksista tutkimus- ja tuotekehitystoiminnan suorituskyvyn johtamisen ja mittaamisen kehittämiseksi. Tutkimuksen laajuus rajattiin neljään case-yritykseen. Tutkimuksessa tehtiin empiirinen puolistrukturoitu kyselytutkimus, strukturoidut haastattelut sekä tulosten arviointi. Tutkimusote oli lähinnä toiminta-analyyttinen, koska tutkimuksessa käsiteltiin organisaation toimintaa, johtamista, ongelmanratkaisua, päätöksentekoprosesseja sekä kehitys- ja muutosprosesseja. Tutkimustulosten perusteella suorituskyvyn johtamista ja mittaamista case-yrityksissä toteutettiin BSC:n yleisten näkökulmien mukaisesti. Tutkimus- ja tuotekehitystoiminnan mittauksen tavoitteet poikkesivat toisistaan niiden toiminnan luonteesta johtuen. Tutkimustoiminnassa suorituskyvyn mittauksen tavoitteet painottuivat lähinnä innovatiivisuuteen, oppimiseen ja motivointiin. Tuotekehitystoiminnassa mittauksen tavoitteet olivat enemmän diagnostisia. Tuotekehitystoiminnassa tavoitteena olivat projektien seurantaan, toiminnan tulostenarviointiin, talouteen sekä koordinaatioon ja kommunikointiin liittyvät tekijät. Tutkimustulosten perusteella puutteita havaittiin suorituskyvyn viestinnän riittävyydessä ja vaikuttavuudessa, mittaustiedon hyödynnettävyydessä, aineettoman pääoman mittauksessa, projektien arviointikriteerien viestinnässä, prosessien selkeydessä ja toimivuudessa, projektiosaamisessa, suorituskyvyn mittauksessa, vaikutusmahdollisuuksissa, johtamisessa ja esimiestyössä.

Understanding how a spiritual retreat enhances the search for meaning and fulfillment in a large corporate workplace /

This study explored the concept of a spiritual retreat for frontline employees of a large corporate call centre. During a 1 day retreat, 4 call centre employees were introduced to various meditation and retreat activities. Follovsdng the retreat the participants were asked to incorporate the various meditations and activities into their workplace. The participants kept journals throughout the study in an effort to determine what occurred when these practices were transferred from the retreat setting to the workplace. This study examined how a working spirituality enhances one's sense of fulfillment, defined by certain critical elements: relationship, awareness, ritual, internal commitment, and choice. Although the retreat was a successful means of exploring these elements, the degree to which each employee could benefit from them was determined by the extent of their internal commitment not only to themselves, but also to their jobs.

Large amplitude motions in the S1 state of formic acid /

A detailed theoretical investigation of the large amplitude motions in the S, excited electronic state of formic acid (HCOOH) was done. This study focussed on the the S, «- So electronic band system of formic acid (HCOOH). The torsion and wagging large amplitude motions of the S, were considered in detail. The potential surfaces were simulated using RHF/UHF ab-initio calculations for the two electronic states. The energy levels were evaluated by the variational method using free rotor basis functions for the torsional coordinates and harmonic oscillator basis functions for the wagging coordinates. The simulated spectrum was compared to the slit-jet-cooled fluorescence excitation spectrum allowing for the assignment of several vibronic bands. A rotational analysis of certain bands predicted that the individual bands are a mixture of rotational a, b and c-type components.The electronically allowed transition results in the c-type or Franck-Condon band and the electronically forbidden, but vibronically allowed transition creates the a/b-type or Herzberg-Teller components. The inversion splitting between these two band types differs for each band. The analysis was able to predict the ratio of the a, b and c-type components of each band.

Behaviour and life history of a large carpenter bee (Xylocopa virginica) in the northern extent of its range

Large carpenter bees (Hymenoptera: Apidae: Xylocopa) have traditionally been thought of as exhibiting solitary or occasionally communal colony social organization. However, studies have demonstrated more complex fonns of social behaviour in this genus. In this document, I examine elements ofbehaviour and life history in a North American species at the northern extreme of its range. Xylocopa virginica was found to be socially polymorphic with both solitary and meta-social or semi-social nests in the same population. In social nests, there is no apparent benefit from additional females which do not perfonn significant work or guarding. I found that the timing of life-history events varies between years, yet foraging effort only differed in the coldest and wettest year of2004 the study. Finally, I that male X virginica exhibit female defence polygyny, with resident and satellite males. Resident males maintain their territories through greater aggression relative to satellites.