WATER-SOLUBLE AND AMPHIPHILIC POLYMERS .7. SYNTHESIS AND CHARACTERIZATION OF A NOVEL COMB-TYPE AMPHIPHILIC POLYMER PAMC16S

A novel comb-like amphiphilic polymer, poly (2-acrylamidohexadecylsulfonic acid) (PAMC16S), was synthesized by free radical polymerization of the corresponding amphiphilic monomer in 1,4-dioxane-water mixed solvents. Depending on the ratio of water/dioxane in the solvent, the reaction proceeded by either precipitation polymerization or micellar polymerization. The molecular weight of the polymer obtained under similar conditions decreased and subsequently increased with the increase of water content in the mixed solvent. The polyion nature of PAMC16S was confirmed by viscosity data of ethanolic solutions. In addition, the polymer was characterized by solubility, IR, TG and wide angle X-ray diffraction methods.

AMPEROMETRIC DETECTION AT A CARBON-FIBER ARRAY RING GLASSY-CARBON DISK ELECTRODE IN A WALL-JET CELL

A wall-jet cell incorporating a carbon fibre array ring/glassy-carbon disk electrode has been constructed, and characterized by the cyclic voltammetry and flow-injection techniques. The ring (composed of several microdisks) and glassy-carbon disk electrode, can be used separately for different purposes, e.g., detection in solution without a supporting electrolyte, collection/shielding detection with dual-electrode and voltammetric/amperometric detection with series dual-electrode. The electrode shows better collection and shielding effects than usual ring-disk electrode in quiescent solution and the series dual-electrode in a thin-layer flow-through cell. The detection limit at the ring electrode is comparable with that at a conventional-size electrode, and has been used in the mobile phase without a supporting electrolyte, proving to be a promising detector for normal-phase liquid chromatography.

SPECTROELECTROCHEMISTRY WITH CYLINDRICAL CARBON-FIBER MICROELECTRODES

Transmittance spectroelectrochemistry can be performed using a group of cylindrical microelectrodes. A dependence of absorbance on electrolytic charge during the potential step was derived. The rate constant of catalytic reaction of the ferrocyanide-ascorbic acid system was determined using single potential step-open circuit relaxation chronoabsorptometry. This is the first report that the reaction can still be considered as a pseudo-first-order reaction when the concentration of ascorbic acid is close to and even slightly lower than the concentration of ferrocyanide. The determined rate constant is in agreement with the reported value. The reason is that the diffusion of ascorbic acid toward electrode surface is contractive and the diffusion of the electrogenerated ferricyanide from the electrode surface to the bulk of solution is expansive.

A NEW KIND OF FLOW-THROUGH ELECTROCHEMICAL DETECTOR USING CARBON-FIBER ELECTRODE FOR LIQUID-CHROMATOGRAPHY

An electrochemical detector which was constracted by using a carbon fibre electrode in a flow-through cell was connected with a liquid chromatographic column. Thus a sensitive,

SOLUBLE POLY-(O,M)-TOLUIDINE

The soluble poly-o-toluidine (POT) and poly-m-toluidine (PMT) have been success- fully prepared under controlled conditions of polymerization and post-treatment, and characterized by IR, VPO and elemental analysis. It is found that the soluble and insol- uble polymers obtained with two different methods (Ⅰ and Ⅱ) have the similar conduc- tivity and molecular chain structure. VPO measurement shows that the number average molecular weight (Mn) of soluble polymers is about 3000. The mole ratio o...

Highly soluble and stable recombinant holo-phycocyanin alpha subunit expressed in Escherichia coli

C-phycocyanin (Cpc) is one of the phycobiliproteins with highly fluorescent and various pharmacological activities Holo-Cpc-alpha Subunit (holo-CpcA) expressed in Escherichia colt resulted in low yield and tended to aggregate after purification in this Study, we constructed a new plasmid coding holo-CpcA fused with hexahistidine and maltose-binding protein tag, which designated as HMCpcA. to Improve Its Solubility and stability without the Impairment of its spectra anti fluorescent properties HMCpcA was significantly more stable over time and a wider range of pH as compared to holo-CpcA. In addition. both the solubility and yields of HMCpcA increase significantly We here provided an example to demonstrate that MBP could also Improve the stability of the protein it fused while it has been reported as a soluble fusion partner before. This novel fluorescent protein will facilitate the large-scale production and be potentially applicable for the development Of fluorescent probes, as well as antioxidant agents (C) 2009 Elsevier B V. All rights reserved