932 resultados para Shimura varieties Torelli locus
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2014
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La maladie de Hirschsprung est une affection congnitale de la motilit intestinale caractrise par un segment aganglionnaire dans le clon terminal. Un criblage gntique par mutation insertionnelle alatoire chez la souris nous a permis didentifier la ligne transgnique Spot dont les homozygotes souffrent de mgaclon aganglionnaire. Lanalyse dintestins dembryons mutants a rvl une baisse de prolifration et un dlai de migration des cellules de la crte neurale entriques (CCNe) prognitrices dus leur diffrenciation gliale prcoce, entrainant un dfaut de colonisation de lintestin et une aganglionose du clon. Le squenage du gnome Spot indique que le transgne sest insr lintrieur du locus K12-Nr2f1 sur le chromosome 13, une rgion dpourvue de gnes pralablement associs la maladie, perturbant galement une squence non-codante trs conserve dans lvolution. K12 est un gne dARN long non codant (ARNlnc) et antisens du gne Nr2f1, lui-mme impliqu dans la gliognse du systme nerveux central. Le squenage du transcriptome des CCN a montr une surexpression de Nr2f1 et des formes courtes de K12 chez Spot et des essais lucifrase ont rvl lactivit rpressive de llment conserv. Nous avons observ lexpression de K12 dans les CCNe et sa localisation subcellulaire dans des zones transcriptionnellement actives du noyau. Avec lmergence des ARNlnc rgulateurs, ces donnes nous permettent de pointer deux nouveaux gnes candidats associs une diffrenciation gliale prmature du SNE menant au mgaclon aganglionnaire, en supposant que la rgulation de Nr2f1 se fait par son antisens, K12.
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Regulatory Focus Theory predicts that the motivation to self-regulate goal-directed thought and behavior depends on two distinct regulation strategies: a promotion focus based on attaining gains and a prevention focus based on avoiding losses. This study took a social-cognitive approach predicting that regulatory focus has an impact on how family startups (several family related founders) explore new ideas, exploit old certainties and achieve the balance of both (ambidexterity), compared to lone founder startups (only one founder present). It was proposed that the social context of family ties among founders leads them to a prevention focus concerned with avoiding the loss of the socio-emotional benefits of those ties. In order to avoid such a loss, family founders were expected to increase their risk perceptions and thus, explore less than lone founders, who lack such socio-emotional ties. It was also proposed that two commonly used psychological traits in entrepreneurship research --achievement motivation and internal locus of control, predispose entrepreneurs to a promotion focus. Founders with a promotion focus, in turn, were hypothesized to lead startups to more risk-seeking behaviors and to more explorative orientation. The previous argument was used as a springboard to derive hypotheses about ambidexterity (the ability to exploit and explore simultaneously) and survival hazards. Using Regulatory Focus Theory, exploitative orientation, conceptualized as the motivational strength to continue on previous paths of action, was hypothesized to be not significantly different from that of lone founder startups. Taking previous arguments together, lone founder startups were hypothesized to be more ambidextrous than family startups. Finally, ambidexterity and internal locus of control were hypothesized to reduce survival hazards in family startups. The findings suggested that family startups explore less than lone founder startups even after controlling for group effects. Interesting but contradictory findings revealed that internal locus of control have both a positive direct effect and a positive interaction that increases the explorative and ambidextrous orientation gap of family startups over lone founder startups. As expected, ambidexterity and internal locus of control reduced survival hazards on family startups. Implications for practitioners were derived based on a sample of 470 nascent entrepreneurs.
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Brucellosis is endemic in most parts of Egypt, where it is caused mainly by Brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant Brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. For this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 Brucella spp. isolates recovered from domestic ruminants (cattle, buffalo, sheep, and goat) from four governorates during a period of five years (2002-2007) was carried out using microbiological tests and molecular biology techniques (PCR, MLVA-15, and sequencing). Thirteen strains were identified as B. melitensis biovar 3 while all phenotypic and genetic techniques classified the remaining isolates as B. abortus (n = 2) and B. suis biovar 1 (n = 2). MLVA-15 yielded a high discriminatory power (h = 0.801), indicating a high genetic diversity among the B. melitensis strains circulating among domestic ruminants in Egypt. This is the first report of the isolation of B. suis from cattle in Egypt which, coupled with the finding of B. abortus, suggests a potential role of livestock as reservoirs of several zoonotic Brucella species in the region.
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High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.
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La maladie de Hirschsprung est une affection congnitale de la motilit intestinale caractrise par un segment aganglionnaire dans le clon terminal. Un criblage gntique par mutation insertionnelle alatoire chez la souris nous a permis didentifier la ligne transgnique Spot dont les homozygotes souffrent de mgaclon aganglionnaire. Lanalyse dintestins dembryons mutants a rvl une baisse de prolifration et un dlai de migration des cellules de la crte neurale entriques (CCNe) prognitrices dus leur diffrenciation gliale prcoce, entrainant un dfaut de colonisation de lintestin et une aganglionose du clon. Le squenage du gnome Spot indique que le transgne sest insr lintrieur du locus K12-Nr2f1 sur le chromosome 13, une rgion dpourvue de gnes pralablement associs la maladie, perturbant galement une squence non-codante trs conserve dans lvolution. K12 est un gne dARN long non codant (ARNlnc) et antisens du gne Nr2f1, lui-mme impliqu dans la gliognse du systme nerveux central. Le squenage du transcriptome des CCN a montr une surexpression de Nr2f1 et des formes courtes de K12 chez Spot et des essais lucifrase ont rvl lactivit rpressive de llment conserv. Nous avons observ lexpression de K12 dans les CCNe et sa localisation subcellulaire dans des zones transcriptionnellement actives du noyau. Avec lmergence des ARNlnc rgulateurs, ces donnes nous permettent de pointer deux nouveaux gnes candidats associs une diffrenciation gliale prmature du SNE menant au mgaclon aganglionnaire, en supposant que la rgulation de Nr2f1 se fait par son antisens, K12.
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We compute the E-polynomials of the moduli spaces of representations of the fundamental group of a complex curve of genus g = 3 into SL(2, C), and also of the moduli space of twisted representations. The case of genus g = 1, 2 has already been done in [12]. We follow the geometric technique introduced in [12], based on stratifying the space of representations, and on the analysis of the behaviour of the E-polynomial under fibrations.
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In this study 15 hazelnut varieties existing in a collection of Viseu Agricultural Station were evaluated. The nuts were studied in respect of their morphological characteristics, such as fruit and kernel weight, index of compression and of shape and shell thickness. The study was complemented with analysis of physical properties such as colour and texture, and the determination of moisture content and water activity, given the importance that these parameters take in the conservation capacity of the fruits. All experiments followed standard methods, being also used the following equipment: texturometer, colorimeter and hygrometer. The results obtained allowed to know the expectable ranges for each color parameters in the shell, film and kernels: L*, a*, b* chroma and hue, having been found statistically significant differences among the cultivars studied. As regards the textural parameters evaluated by crust crushing and crumb cutting tests (hardness, friability and resilience) there were also significant differences. Evaluation of moisture was of great importance because confirmed that the solar drying, used to extract the excess of moisture from the fruits, was sufficient to reach low values, between 1.66% and 4.52%, being so a guarantee of preservation.
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At Mediterranean regions and particularly in southern Portugal, it is imperative to identify grape varieties more adapted to warm and dry climates in order to overcome future climatic changes. Two Vitis vinifera genotypes, Aragonez (syn. Tempranillo) and Trincadeira, were selected to assess their physiological responses to soil water stress. Vines were subjected to four irrigation regimes: irrigated during all phenological cycle, non-irrigated during all phenological cycle, non irrigated until veraison, irrigated after veraison. Predawn leaf water potential was much higher in Trincadeira than Aragonez in non- irrigated plants. This result is in accordance with its higher stomatal control efficiency in this variety (Trincadeira). Photosynthetic capacity (Amax at saturating light intensity) decreased due to stomatal and biochemical limitations under water stress. However, recovery capacity of leaf water status after irrigation was faster in Trincadeira. Yield and yield x Brix increased when irrigation occurred after veraison, particularly in Trincadeira. These results show that Trincadeira presents a drought adaptation than Aragonez. Ratio of variable to maximum fluorescence Fv/Fm and total leaf chlorophyll related with leaf water potential for both species. Reflectance Normalized Difference Vegetation Index (NDVI705), Red Edge Inflexion Point Index and Photochemical Reflectance Index were related with irrigation treatment. Relative water content and specific leaf area were similar between varieties. In conclusion, we suggested that there is variation among the genotypes and the main physiological parameters for variety selection, for drought, were leaf water potential, stomatal conductance and reflectance indexes.
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The flavonoids (including anthocyanins) are wine compounds with important anti-oxidant activity, protecting the cells against oxidative processes, preventing cardiovascular and neurodegenerative diseases, cancer, among others (Antoniolli et al. 2015; Castaeda-Ovando et al. 2009; Hosu et al. 2014; Huang et al. 2009; Kong et al. 2003). Anthocyanins in grapes at harvest are determinant to red wine quality and their development in the grape must be characterised in order to determine the most suitable date for the harvest. Thus the aim of this research is the evaluation of anthocyanins composition in two red wine grape varieties from vraison continuing through ripening. Anthocyanins were quantified by high resolution liquid chromatography (HPLC-DAD). Additionally, the total phenols content were quantified by UV-Vis Spectrometry. The anthocyanins profile evolution may be dependent on the variety and ripening phase. During ripening grape samples have shown an increase of coumaryl derivatives. This information may lead us to understand the anthocyanins biosynthesis pathway in different grape varieties. The development of anthocyanins from the vraison seems to follow a pattern that coincides with the increasing accumulation of soluble sugars.
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A praxis em teatro e comunidade opera e mobiliza diferentes cincias e metodologias em vis transversal. Seu movimento busca contribuir para projetos artsticos e sociais que agregam fenmenos que instauram a compreenso de quadros de referncias - estticas e comunitrias. Para que se efetivem, h a necessidade de determinao dos espaos nos quais ocorre o ato teatral. A proposta desse artigo , a partir da compreenso fenomenolgica, investigar o uso de espaos pblicos e comunitrios como lcus para a ao teatral em contextos especficos. Nos exemplos das pesquisas no Brasil e Portugal d-se conta de como essas prticas dialogam com seus grupos de interesse. A partir dos resultados, indicam-se possveis contribuies metodolgicas na formao de Artistas Pedagogos e sua atuao em contextos socio-educacionais.
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A cultura da banana tem baixa diversidade gentica, tornando a espcie susceptvel a doenas dizimadoras como a Sigatoka negra. No entanto, a adoo de novas variedades necessita de avaliaes agronmicas e fsico-qumicas. Neste estudo, as variedades de banana, resistentes Sigatoka negra, foram caracterizadas e comparadas com a variedade tradicional (Grand Naine). Cada variedade foi avaliada considerando-se critrios relevantes para a agroindstria, como pH, slidos solveis totais, acidez total titulvel, relao SST/ATT, acares totais, acares redutores e no redutores, umidade, slidos totais e rendimento no processamento. A variedade Thap Maeo apresentou-se como a variedade mais potencial para substituio da Gran Naine na indstria, com altos teores de slidos solveis totais, acares redutores, acares totais e umidade. As variedades Caipira e FHIA 2 tambm podem substituir a Grand Naine. Na anlise de agrupamentos, verificou-se que a variedade Grand Naine esteve muito prxima das variedades do subgrupo Gros Michel (Bucaneiro, Ambroisa e Calipso) e tambm da variedade Caipira, apresentando no seu genoma o grupo AAA. Conclui-se que h opes de variedades resistentes para substituio da variedade tradicional, nas regies afetadas pela Sigatoka-negra.
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Based upon qualitative parameters experiments, this study aims to investigate how the elements of the environment, where the coffee is produced, contribute to the final quality of the product. For the analyses, it was used approximately one kilogram of coffee cherry samples collected in 14municipalities previously chosen on the East side of the Minas Gerais State, Brazil. The coffee cherry samples were collected and analyzed for each of the two varieties in four levels of altitude for each exposure side of the mountain in relation to the Sun. The quality of the coffee was evaluated through the analysis of its physical characteristics and sensory analysis, popularly known as "Test of drink or Cupping" carried out by three tasters that belonging to the group of Q -Graders, according to the rules of national and international competitions of the Brazilian Association of Special Coffees (BSCA). Were performed analysis by means descriptive statistics, analysis of variance and multivariate analysis, all of them aiming to study the individual sensor y characteristics of quality of the coffee beverage from the ?Matas de Minas? region. Path coefficient analysis also was carried out for the partition of the phenotypic correlation coefficients into measures of direct and indirect effects, in order to determine the individual sensory characteristics that playeda major role in the beverage final score. The results demonstrat e that it is not possible to concl usively establish the differences among coffees evaluated with basis on varieties and environmental factors previously cited. It can be concluded that it is not recommended to associate the quality of coffee only to a specific factor whether from the environment or being it a specific of the culture of coffee. However, the cafes that were evaluated had intrinsic quality, which were derived from the specific characteristics of the ?Matas de Minas? region where they were grown.
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The tissue kallikreins are serine proteases encoded by highly conserved multigene families. The rodent kallikrein (KLK) families are particularly large, consisting of 13 26 genes clustered in one chromosomal locus. It has been recently recognised that the human KLK gene family is of a similar size (15 genes) with the identification of another 12 related genes (KLK4-KLK15) within and adjacent to the original human KLK locus (KLK1-3) on chromosome 19q13.4. The structural organisation and size of these new genes is similar to that of other KLK genes except for additional exons encoding 5 or 3 untranslated regions. Moreover, many of these genes have multiple mRNA transcripts, a trait not observed with rodent genes. Unlike all other kallikreins, the KLK4-KLK15 encoded proteases are less related (2544%) and do not contain a conventional kallikrein loop. Clusters of genes exhibit high prostatic (KLK2-4, KLK15) or pancreatic (KLK6-13) expression, suggesting evolutionary conservation of elements conferring tissue specificity. These genes are also expressed, to varying degrees, in a wider range of tissues suggesting a functional involvement of these newer human kallikrein proteases in a diverse range of physiological processes.
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This article presents the findings of a study of the psychological variables that discriminate between high and low omitters on a high-stakes achievement test using a short-response format. Data were obtained from a questionnaire administered to a random sample (N = 1,908) of students prior to sitting the 1997 Queensland Core Skills (QCS) Test (N = 29,273). Fourteen psychological variables were measured including test anxiety (four subscales), emotional stability, achievement motivation, self-esteem, academic self-concept, self-estimate of ability, locus of control (three subscales), and approaches to learning (two subscales). The results were analyzed using descriptive discriminant analysis and suggested that the psychological predictors of the propensity to omit short-response items include test-irrelevant thinking and academic self-concept, with sex of candidate being a mediating variable.
