Timing of visual stimuli in V1 and V5/MT: fMRI and TMS

Time perception is used in our day-to-day activities. While we understand quite well how our brain processes vision, touch or taste, brain mechanisms subserving time perception remain largely understudied. In this study, we extended an experiment of previous master thesis run by Tatiana Kenel-Pierre. We focused on time perception in the range of milliseconds. Previous studies have demonstrated the involvement of visual areas V1 and V5/MT in the encoding of temporal information of visual stimuli. Based on these previous findings the aim of the present study was to understand if temporal information was encoded in V1 and extrastriate area V5/MT in different spatial frames i.e., head- centered versus eye-centered. To this purpose we asked eleven healthy volunteers to perform a temporal discrimination task of visual stimuli. Stimuli were presented at 4 different spatial positions (i.e., different combinations of retinotopic and spatiotopic position). While participants were engaged in this task we interfered with the activity of the right dorsal V1 and the right V5/MT with transcranial magnetic stimulation (TMS). Our preliminary results showed that TMS over both V1 and V5/MT impaired temporal discrimination of visual stimuli presented at specific spatial coordinates. But whereas TMS over V1 impaired temporal discrimination of stimuli presented in the lower left quadrant, TMS over V5/MT affected temporal discrimination of stimuli presented at the top left quadrant. Although it is always difficult to draw conclusions from preliminary results, we could tentatively say that our data seem to suggest that both V1 and V5/MT encode visual temporal information in specific spatial frames.

Semantic domain and grammatical class effects in the picture-word interference paradigm

The role of grammatical class in lexical access and representation is still not well understood. Grammatical effects obtained in picture-word interference experiments have been argued to show the operation of grammatical constraints during lexicalization when syntactic integration is required by the task. Alternative views hold that the ostensibly grammatical effects actually derive from the coincidence of semantic and grammatical differences between lexical candidates. We present three picture-word interference experiments conducted in Spanish. In the first two, the semantic relatedness (related or unrelated) and the grammatical class (nouns or verbs) of the target and the distracter were manipulated in an infinitive form action naming task in order to disentangle their contributions to verb lexical access. In the third experiment, a possible confound between grammatical class and semantic domain (objects or actions) was eliminated by using action-nouns as distracters. A condition in which participants were asked to name the action pictures using an inflected form of the verb was also included to explore whether the need of syntactic integration modulated the appearance of grammatical effects. Whereas action-words (nouns or verbs), but not object-nouns, produced longer reaction times irrespective of their grammatical class in the infinitive condition, only verbs slowed latencies in the inflected form condition. Our results suggest that speech production relies on the exclusion of candidate responses that do not fulfil task-pertinent criteria like membership in the appropriate semantic domain or grammatical class. Taken together, these findings are explained by a response-exclusion account of speech output. This and alternative hypotheses are discussed.

A conceptual model for grid learning services automatic composition

Peer-reviewed

Eristekourujen ponttauslaitteen suunnittelu

Diplomityössä suunnitellaan eristekourujen ponttauslaite, jolla pystytään ponttaamaan eristekouruja. Pontattavien eristekourujen pituus ja halkaisija muuttuvat. Suunnittelun lähtökohtana on nykyisin käytössä olevien ponttauslaitteiden ergonomia- ja työturvallisuusongelmat. Työssä käydään läpi erilaisia mahdollisuuksia ponttauslaitteen toteutukseen ja kehitellään kaksi periaatteellista ratkaisua toimiviksi kokoonpanoiksi. Kokoonpanoista valitaan perustellusti parempi vaihtoehto jatkokehitykseen ja se kehitellään valmiiksi ponttauslaitteeksi. Työ käsittää laitteen mekaniikkasuunnittelun, automaatiosuunnittelu rajataan työn ulkopuolelle. Työn eteneminen seuraa järjestelmällisen koneensuunnitteluprosessin vaiheita. Lopputuloksena suunniteltiin laite, joka toteuttaa sille asetetut suoritusvaatimukset. Suunniteltu laite on turvallinen ja ergonomiset vaatimukset täyttävä. Lisäksi laitteen rungolle tehtiin värähtelytarkasteluja, joiden perusteella analysoitiin ominais- ja herätetaajuuksien suhteita sekä rungon jäykistämisen vaikutuksia.

Type II aromatic polyketide biosynthetic tailoring enzymes: diversity and adaptation in Streptomyces secondary metabolism.

Members of the bacterial genus Streptomyces are well known for their ability to produce an exceptionally wide selection of diverse secondary metabolites. These include natural bioactive chemical compounds which have potential applications in medicine, agriculture and other fields of commerce. The outstanding biosynthetic capacity derives from the characteristic genetic flexibility of Streptomyces secondary metabolism pathways: i) Clustering of the biosynthetic genes in chromosome regions redundant for vital primary functions, and ii) the presence of numerous genetic elements within these regions which facilitate DNA rearrangement and transfer between non-progeny species. Decades of intensive genetic research on the organization and function of the biosynthetic routes has led to a variety of molecular biology applications, which can be used to expand the diversity of compounds synthesized. These include techniques which, for example, allow modification and artificial construction of novel pathways, and enable gene-level detection of silent secondary metabolite clusters. Over the years the research has expanded to cover molecular-level analysis of the enzymes responsible for the individual catalytic reactions. In vitro studies of the enzymes provide a detailed insight into their catalytic functions, mechanisms, substrate specificities, interactions and stereochemical determinants. These are factors that are essential for the thorough understanding and rational design of novel biosynthetic routes. The current study is a part of a more extensive research project (Antibiotic Biosynthetic Enzymes; www.sci.utu.fi/projects/biokemia/abe), which focuses on the post-PKS tailoring enzymes involved in various type II aromatic polyketide biosynthetic pathways in Streptomyces bacteria. The initiative here was to investigate specific catalytic steps in anthracycline and angucycline biosynthesis through in vitro biochemical enzyme characterization and structural enzymology. The objectives were to elucidate detailed mechanisms and enzyme-level interactions which cannot be resolved by in vivo genetic studies alone. The first part of the experimental work concerns the homologous polyketide cyclases SnoaL and AknH. These catalyze the closure of the last carbon ring of the tetracyclic carbon frame common to all anthracycline-type compounds. The second part of the study primarily deals with tailoring enzymes PgaE (and its homolog CabE) and PgaM, which are responsible for a cascade of sequential modification reactions in angucycline biosynthesis. The results complemented earlier in vivo findings and confirmed the enzyme functions in vitro. Importantly, we were able to identify the amino acid -level determinants that influence AknH and SnoaL stereoselectivity and to determine the complex biosynthetic steps of the angucycline oxygenation cascade of PgaE and PgaM. In addition, the findings revealed interesting cases of enzyme-level adaptation, as some of the catalytic mechanisms did not coincide with those described for characterised homologs or enzymes of known function. Specifically, SnoaL and AknH were shown to employ a novel acid-base mechanism for aldol condenzation, whereas the hydroxylation reaction catalysed by PgaM involved unexpected oxygen chemistry. Owing to a gene-level fusion of two ancestral reading frames, PgaM was also shown to adopt an unusual quaternary sturucture, a non-covalent fusion complex of two alternative forms of the protein. Furthermore, the work highlighted some common themes encountered in polyketide biosynthetic pathways such as enzyme substrate specificity and intermediate reactivity. These are discussed in the final chapters of the work.

Secure logarithmic audio watermarking scheme based on the human auditory system

Peer-reviewed

Ontologia de LinkedIn : El primer pas per inferir informació a partir de les ofertes de feina

Amb aquest treball de final de carrera de la titulació d'enginyeria tècnica en informàtica de gestió es pretén fer una primera aproximació al món de l'anàlisi semàntic de webs. Consisteix, per una banda, en la creació d'una ontologia per emmagatzemar informació provinent de la web de LinkedIn de manera que després pugui ser analitzada i permeti filtrar les dades de manera pràctica evitant l'excés d'informació no útil. Per altra banda, el treball inclou el desenvolupament d'una aplicació per a l'obtenció de la informació de la web de LinkedIn de manera automàtica, i un mètode per a la importació a l'ontologia creada.

Ontologia del LinkedIn : Primer pas per inferir informació a partir de les ofertes de feina

El cas que ens ocupa és la xarxa social LinkedIn. La quantitat d'ofertes i demandes de feina fan a vegades difícil extreure'n la informació necessària. Es vol crear una ontologia per tal de nodrir les dades presents en la web LinkedIn amb informació semàntica. Mitjançant el programari Protégé es pretén crear aquesta ontologia i, mitjançant les API que ofereix LinkedIn, un mitjà per inferir informació respecte a les ofertes i demandes de feina.

El conflicto de Gamonal

En la provincia de Burgos, España, un movimiento vecinal en contra de un proyecto urbanístico acabó derivando en Enero de 2014 en un acontecimiento político con gran seguimiento por parte de los medios de comunicación tradicionales y las redes sociales. La relevancia política de un conflicto en un primer momento local empezó a aumentar y a generar diferentes lecturas e interpretaciones, tanto desde dentro del movimiento como en el resto del Estado español. En este trabajo analizamos las redes de interacciones, los marcos interpretativos del movimiento de protesta y cómo éstos fueron modulándose con base a las distintas fases del conflicto. El análisis sistemático del contenido y las redes sociales de Twitter se ha combinado con siete entrevistas realizadas a participantes en el conflicto. A partir de los datos obtenidos y su análisis, se puede concluir que se dieron cambios importantes en los marcos del movimientohacia perspectivas nacionales y globales en relación con una expansión ydesbordamiento de las redes de interacción que sustentaban el movimiento.

Modelo de comportamiento en sevicio para estructuras reticulares de hormigón. Parte I: Descripción del modelo y ejemplos de aplicación

A general method for instantaneous and time-dependent serviceability analysis of plane concrete frames is presented. The methodology is based in an extension of the classic matrix formulation for bars. The main aspects influencing the behaviour of the structural concrete are considered: cracking, creep, shrinkage or prestress losses. To simulate the effect of cracking a smeared model (developed in Part II) based on the modification of the tensile branch of the concrete stress-strain relationship is adopted. The general approach considered permits the application to different materials and constitutive laws. Sequential construction (sectional and structural), incorporation of reinforcement, consideration of the loads history; placing and removing shores, and restraining or releasing in boundary conditions are considered. Some examples are included to highlight the capabilities of the model

Deformation of Gabor systems

We introduce a new notion for the deformation of Gabor systems. Such deformations are in general nonlinear and, in particular, include the standard jitter error and linear deformations of phase space. With this new notion we prove a strong deformation result for Gabor frames and Gabor Riesz sequences that covers the known perturbation and deformation results. Our proof of the deformation theorem requires a new characterization of Gabor frames and Gabor Riesz sequences. It is in the style of Beurling's characterization of sets of sampling for bandlimited functions and extends significantly the known characterization of Gabor frames 'without inequalities' from lattices to non-uniform sets.

Fourier-based registration for robust forward-looking sonar mosaicing in low-visibility underwater environments

Vehicle operations in underwater environments are often compromised by poor visibility conditions. For instance, the perception range of optical devices is heavily constrained in turbid waters, thus complicating navigation and mapping tasks in environments such as harbors, bays, or rivers. A new generation of high-definition forward-looking sonars providing acoustic imagery at high frame rates has recently emerged as a promising alternative for working under these challenging conditions. However, the characteristics of the sonar data introduce difficulties in image registration, a key step in mosaicing and motion estimation applications. In this work, we propose the use of a Fourier-based registration technique capable of handling the low resolution, noise, and artifacts associated with sonar image formation. When compared to a state-of-the art region-based technique, our approach shows superior performance in the alignment of both consecutive and nonconsecutive views as well as higher robustness in featureless environments. The method is used to compute pose constraints between sonar frames that, integrated inside a global alignment framework, enable the rendering of consistent acoustic mosaics with high detail and increased resolution. An extensive experimental section is reported showing results in relevant field applications, such as ship hull inspection and harbor mapping

Processat de vídeo aplicat a la robòtica

Aquest projecte consisteix en acoblar una càmera web uEye en una de les extremitats d'un robot industrial (ABB Irc5). Aquest robot, es mourà en cas què es detecti una cara a través de la webcam. L'objectiu és intentar que la cara es situï sempre al mig de la imatge captada per la càmera. Podem dividir les tasques d'aquest projecte amb 4 fases diferents. A continuació expliquem les diferents etapes. La Càmera uEye s'encarrega de capturar imatges i passar-les a un PC utilitzant Python. La funció de Detecció de Cares ens indica la posició i dimensions de la cara. Tot seguit, la funció LabJack s'encarrega de canviar els valors digitals de les 5 senyals mitjançant programació amb Python. Per últim el Robot ABB interpreta el valor d'aquestes 5 senyals i es desplaça mitjançant petits increments amb l'objectiu de situar la cara al mig de la imatge capturada.