Label-free oligonucleotide biosensor based on dual-peak long period fiber grating

We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.

Study of KJ-66 micro gas turbine compressor: steady and unsteady Reynolds-averaged Navier-Stokes approach

Numerical study on the compressor stage of a KJ-66 micro gas turbine was conducted in this paper through both steady and unsteady Reynolds-averaged Navier–Stokes. The study was conducted for the numerical prediction of micro gas turbine compressor performance at various operation conditions, with special attention given to the transient flow behaviors during compressor operation. The numerical results showed reasonable agreements with experimental data while providing predictions for the charting of compressor performance map at various operation speeds. The simulation results indicated that the increase of operation speed from 80 k r/min to 117 k r/min would leads to an increased peak total pressure ratio from 1.54 to 1.96, while decreasing the peak adiabatic efficiency from 0.73 to 0.55. This paper also provided discussion on details of transient flow field within the compressor stage as well as demonstrated the smooth flow transition through rotor–stator interactions.