Label-free oligonucleotide biosensor based on dual-peak long period fiber grating
Contribuinte(s) |
Gannot, Israel |
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Data(s) |
07/03/2016
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Resumo |
We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity. |
Formato |
application/pdf |
Identificador |
Chen, Xianfeng; Liu, Chen; Cardoso, Marcos R.; Mendonça, Cleber R.; Nagel, David A.; Hine, Anna V. and Zhang, Lin (2016). Label-free oligonucleotide biosensor based on dual-peak long period fiber grating. IN: Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI. Gannot, Israel (ed.) SPIE Proceedings . Bellingham, WA (US): SPIE. |
Publicador |
SPIE |
Relação |
http://eprints.aston.ac.uk/29709/ |
Tipo |
Book Section NonPeerReviewed |