Amphibia, anura, hylidae, hypsiboas raniceps cope, 1862: Distribution extension

Hypsiboas raniceps is a large treefrog typically found in the Cerrado and Caatinga biomes. Here we provide information about new records of this species in the Atlantic Forest and raise some hypotheses that could explain the late record of this species in this biome. © 2010 Check List and Authors.

Existe utilização efetiva dos recursos vegetais conhecidos em comunidades caiçaras da Ilha do Cardoso, estado de São Paulo, Brasil?

This study aims to investigate if plant resources are effective used by caiçaras' communities of the Cardoso Island. We also want to verify if the biogeografic origin of plants, their uses and socioeconomic factors influence the locals' knowledge. We carried out 51 semi-structured interviews with people who have been living there for at least 5 years, who were older than 18. We concluded that the interviewees' knowledge is still diverse and that most of the plants known are used by them (82%). Native plants are largely known, while the exotic ones are the most used (95%). Knowledge and use vary according to the use of plants, although they were very similar, considering the interviewees' age and gender. We also realized that housewives use a large quantity of medicinal plants, which are mainly the exotic ones. Fishermen know and use native plants, usually for handicraft purposes. The environmental guides and people, whose job is tourism related, have a similar knowledge, especially about medicinal plants, which are less used when compared with the other professional activities.

Dinámica del suelo y estoque de carbono después de diez años de la restauración de tierras degradadas usando especies arbóreas del bosque atlántico

Brazil's Atlantic Forest ecosystem has been greatly affected by land use changes, with only 11.26% of its original vegetation cover remaining. Currently, Atlantic Forest restoration is receiving increasing attention because of its potential for carbon sequestration and the important role of soil carbon in the global carbon balance. Soil organic matter is also essential for physical, chemical and biological components of soil fertility and forest sustainability. This study evaluated the potential for soil recovery in contrasting restoration models using indigenous Atlantic Forest tree species ten years after their establishment. The study site is located in Botucatu municipality, São Paulo State-Brazil, in a loamy dystrophic Red-Yellow Argisol site (Typic Hapludult). Four treatments were compared: i) Control (Spontaneous Restoration); ii) Low Diversity (five fast-growing tree species established by direct seeding); iii) High Diversity (mixed plantings of 41 species established with seedlings) and; iv) Native Forest (well conserved neighboring forest fragment). The following soil properties were evaluated: (1) physical-texture, density and porosity; (2) chemical-C, N, P, S, K, Ca, Mg, Al and pH; (3) biological-microbial biomass. Litter nutrient concentrations (P, S, K, Ca and Mg) and C and N litter stocks were determined. Within ten years the litter C and N stocks of the Low Diversity treatment area were higher than Control and similar to those in both the High Diversity treatment and the Native Forest. Soil C stocks increased through time for both models and in the Control plots, but remained highest in the Native Forest. The methods of restoration were shown to have different effects on soil dynamics, mainly on chemical properties. These results show that, at least in the short-term, changes in soil properties are more rapid in a less complex system like the Low Diversity model than in the a High Species Diversity model. For both mixed plantation systems, carbon soil cycling can be reestablished, resulting in increases in carbon stocks in both soil and litter.

Phytoseiidae (Acari: Mesostigmata) on plants of the central region of the Brazilian cerrado

Four surveys of Phytoseiidae species associated with native plants were conducted in ten fragments of Cerrado sensu stricto to determine the species associated with the plants of this region. Twelve of the most common plant species were sampled in each fragment. Twenty six phytoseiid species were recorded during the surveys. Stryphnodendron adstringens was the host plant that had the highest number of species (eight species). Amblyseius neochiapensis Lofego, Moraes and McMurtry 2000 and Neoseiulus tunus (De Leon) 1967 were the most common phytoseiid species. These species occurred on 24 and 20 host species, respectively. Approximately 76 % of the phytoseiids found belonged to the subfamily Amblyseiinae Muma, 1961. Almost 27 % of phytoseiid species recorded were associated with only one plant species. In addition, approximately two thirds of species found in this study also occur in the Atlantic Forest. The comparison of our results from the central areas with previous surveys in peripheral areas of the Cerrado showed that only 12 phytoseiid species were common to both areas.

Differential seed germination of a keystone palm (Euterpe edulis) dispersed by avian frugivores

The effectiveness of seed dispersal by vertebrates has been analysed by examining both quantitative and qualitative components (Jordano & Schupp 2000, Schupp et al. 2010). While the quantitative component is relatively easily assessed in the field (e.g. visitation rate, number of fruits eaten per visit), the qualitative component (e.g. fate of dispersed seeds, seed treatment in the digestive system of the disperser) is rarely studied under natural conditions, because it is difficult to measure the effects on seeds once ingested by the dispersers (Cortes et al. 2009). © Cambridge University Press 2012.

Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing

The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity was assessed in 21 individuals from a population (Reserva Ecológica de Guapiaçu, REGUA) locatedin the central region of the Rio de Janeiro State, in Brazil. The mean number of alleles per locus ranged from 3 to 12. Observed and expected heterozygosities ranged from 0.095 to 0.905 and from 0.094 to 0.904, respectively. After using the Bonferroni correction for multiple tests, there was no evidence of linkage disequilibrium between pairs of loci but deviations for Hardy-Weinberg equilibrium were found in 4 loci. We found no evidence for allele dropouts or stuttering, but we detected the presence of null alleles at loci Eb10 and Eb36. These markers will be useful for analyses of fine-scale population structure and determination of relative effects of habitat loss and fragmentation on population genetic variability within species. © FUNPEC-RP.

Functional Redundancy and Complementarities of Seed Dispersal by the Last Neotropical Megafrugivores

Background: Functional redundancy has been debated largely in ecology and conservation, yet we lack detailed empirical studies on the roles of functionally similar species in ecosystem function. Large bodied frugivores may disperse similar plant species and have strong impact on plant recruitment in tropical forests. The two largest frugivores in the neotropics, tapirs (Tapirus terrestris) and muriquis (Brachyteles arachnoides) are potential candidates for functional redundancy on seed dispersal effectiveness. Here we provide a comparison of the quantitative, qualitative and spatial effects on seed dispersal by these megafrugivores in a continuous Brazilian Atlantic forest. Methodology/Principal Findings: We found a low overlap of plant species dispersed by both muriquis and tapirs. A group of 35 muriquis occupied an area of 850 ha and dispersed 5 times more plant species, and 13 times more seeds than 22 tapirs living in the same area. Muriquis dispersed 2.4 times more seeds in any random position than tapirs. This can be explained mainly because seed deposition by muriquis leaves less empty space than tapirs. However, tapirs are able to disperse larger seeds than muriquis and move them into sites not reached by primates, such as large forest gaps, open areas and fragments nearby. Based on published information we found 302 plant species that are dispersed by at least one of these megafrugivores in the Brazilian Atlantic forest. Conclusions/Significance: Our study showed that both megafrugivores play complementary rather than redundant roles as seed dispersers. Although tapirs disperse fewer seeds and species than muriquis, they disperse larger-seeded species and in places not used by primates. The selective extinction of these megafrugivores will change the spatial seed rain they generate and may have negative effects on the recruitment of several plant species, particularly those with large seeds that have muriquis and tapirs as the last living seed dispersers. © 2013 Bueno et al.

Phenotypic variation of leptodactylus cupreus caramaschi, são-pedro and feio, 2008 (Anura, Leptodactylidae)

This study describes for the first time the female of Leptodactylus cupreus and provides new information concerning its geographical distribution, male's morphology and bioacustics. Leptodactylus cupreus, a poorly known species from the Brazilian Atlantic Forest, was originally allocated in the L. mystaceus complex of the L. fuscus species group. Based on morphological observations, we infer that L. cupreus should be in fact related to L. mystacinus, a species that, although assigned to the L. fuscus species group, is not assigned to the L. mystaceus complex. Therefore, we comment the phylogenetic relationships concerning L. cupreus, L. mystaceus and L. mystacinus. © 2013 Magnolia Press.

Avian assemblages in bamboo and non-bamboo habitats in a tropical rainforest

Some species of bird are closely associated with bamboos (bamboo specialists) but community-wide studies comparing the avian assemblages in bamboo and non-bamboo habitats are lacking. Using point counts, we compared the species richness, abundance and composition of the avian assemblages in bamboo and non-bamboo habitats in the Brazilian Atlantic forest. Apart from considering bamboo specialists and non-specialist species, we contrasted birds from different categories of forest dependence, forest strata and diet. We recorded a total of 81 species of birds (74 in bamboo, 55 in non-bamboo habitats), including 15 bamboo specialists. Species richness was greater in bamboo habitats in all categories of diet and forest dependence. Bamboo and non-bamboo habitats had a similar number of canopy species, but bamboo habitats had a greater number of non-canopy species. The abundance of the whole avian community or of each of the dietary categories did not differ between habitats. The overall species composition differed between habitats, with a more homogeneous composition in non-bamboo habitats. A great number of species use bamboo habitats, even if they are not bamboo specialists. The initial expansion of bamboos, forming discrete patches of bamboo within mature forest, represents an intermediate-level disturbance that enhances forest heterogeneity and promotes the diversity of avian communities. © BirdLife Australia 2013.

Leaf anatomical and molecular studies in Bulbophyllum section Micranthae (Orchidaceae) and their implications for systematics

Bulbophyllum section Micranthae comprises 12 species of rupicolous or epiphytic orchids occurring in forests or in open rocky fields in Cerrado/Atlantic Forest ecotones throughout South America. We examined the leaf anatomy of 14 species and compared them with molecular data (nrITS) in phylogenetic analyses. The leaves of Bulbophyllum section Micranthae are characterised by uniseriate epidermis, with periclinal external cell wall thicker than the internal, presence of epicuticular wax, stomata present only on the abaxial surface with suprastomatic chambers, and collateral vascular bundles associated with sclerenchyma fibres. Some of these characters are shared with other rupicolous Orchidaceae species, demonstrating adaptive convergence in xeromorphic habitats. We found some anatomical characteristics with phylogenetic value. Bulbophyllum section Micranthae can be separated into two lineages: those with needle-like leaves, or flat leaves. The analyses show that anatomical characters as well as molecular data may contribute to the development of phylogenetic hypotheses. © 2013 Botanical Society of Sao Paulo.

New Neotropical species of Hygronemobius Hebard, 1913 (Orthoptera: Grylloidea: Nemobiinae), including a brief discussion of male genitalia morphology and preliminary biogeographic considerations of the genus

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

From widespread to microendemic: Molecular and acoustic analyses show that Ischnocnema guentheri (Amphibia: Brachycephalidae) is endemic to Rio de Janeiro, Brazil

Many species of tropical amphibians are restricted to very small ranges, and this microendemism coupled with ongoing habitat loss and susceptibility to emerging pathogens imperils the long-term persistence of these species. Incomplete taxonomic and distributional knowledge may obscure conservation assessment, particularly in putatively widespread species that are typically considered to be of Least Concern in Red List assessments, but that in fact may constitute complexes of partly microendemic species. Such is the case in the Steindachner's Robber Frog, Ischnocnema guentheri which, together with the recently recognized Ischnocnema henselii, is thought to occupy most of the Brazilian Atlantic Forest. To test whether these taxa may constitute a species complex of range-restricted and thus potentially threatened species, we analyzed 160 samples of I. guentheri and/or I. henselii for two molecular markers, 16S rRNA (16S) and recombination activation gene 1 (RAG1). To verify the monophyly of the complex, closely related species were also included in the 16S analysis. Congruent evidence from the molecular data and from analyses of advertisement calls support the existence of six distinct species within the complex: I. guentheri and I. henselii as well as four candidate new species. The lineages are distributed as a mosaic in the Atlantic Forest and are sympatric at some localities without indication of admixture. Their phylogeographical pattern partially agrees with paleo-models for the Atlantic Forest, but also suggests the existence of micro-refugia in less stable areas. I. guentheri, previously considered to be widespread, was found only in its type locality, a reserve within the urban area of Rio de Janeiro city. Although none of the species studied appears highly threatened with extinction, we recommend their IUCN threat status to be re-evaluated carefully for the next comprehensive update of the Red List of Brazil's amphibians. © 2013 Springer Science+Business Media Dordrecht.

Improving methods in gap ecology: Revisiting size and shape distributions using a model selection approach

Questions: We assess gap size and shape distributions, two important descriptors of the forest disturbance regime, by asking: which statistical model best describes gap size distribution; can simple geometric forms adequately describe gap shape; does gap size or shape vary with forest type, gap age or the method used for gap delimitation; and how similar are the studied forests and other tropical and temperate forests? Location: Southeastern Atlantic Forest, Brazil. Methods: Analysing over 150 gaps in two distinct forest types (seasonal and rain forests), a model selection framework was used to select appropriate probability distributions and functions to describe gap size and gap shape. The first was described using univariate probability distributions, whereas the latter was assessed based on the gap area-perimeter relationship. Comparisons of gap size and shape between sites, as well as size and age classes were then made based on the likelihood of models having different assumptions for the values of their parameters. Results: The log-normal distribution was the best descriptor of gap size distribution, independently of the forest type or gap delimitation method. Because gaps became more irregular as they increased in size, all geometric forms (triangle, rectangle and ellipse) were poor descriptors of gap shape. Only when small and large gaps (> 100 or 400m2 depending on the delimitation method) were treated separately did the rectangle and isosceles triangle become accurate predictors of gap shape. Ellipsoidal shapes were poor descriptors. At both sites, gaps were at least 50% longer than they were wide, a finding with important implications for gap microclimate (e.g. light entrance regime) and, consequently, for gap regeneration. Conclusions: In addition to more appropriate descriptions of gap size and shape, the model selection framework used here efficiently provided a means by which to compare the patterns of two different types of forest. With this framework we were able to recommend the log-normal parameters μ and σ for future comparisons of gap size distribution, and to propose possible mechanisms related to random rates of gap expansion and closure. We also showed that gap shape varied highly and that no single geometric form was able to predict the shape of all gaps, the ellipse in particular should no longer be used as a standard gap shape. © 2012 International Association for Vegetation Science.

Checklist of freshwater ichthyofauna from coastal streams of Juréia-Itatins Reserve, Southeastern Brazil

The Juréia-Itatins Ecological Station (JIES) is one of the few protected areas in the state of São Paulo, which harbor a significant portion of the Atlantic forest. Although there is high diversity and endemism of fish species in this region, knowledge of the ichthyofauna in JIES is incipient. A total of 4,809 specimens belonging to six orders, 15 families and 31 species were captured during April 2009 to February 2010, using electrofishing, gillnets and traps. The family Characidae was predominant, mainly due to the abundance of freshwater species such as Astyanax ribeirae, Mimagoniates microlepis, and Hollandichthys multifasciatus. Although the estuarine regions were rarely sampled in this study, six brackish water species were captured. In this study, nine species previously unknown from JIES were discovered. Five endangered fish species, endemic to the Atlantic forest, occur at JIES, thus the presence of protected areas, as a conservation measure, is particularly relevant. © 2013 Check List and Authors.

Functional extinction of birds drives rapid evolutionary changes in seed size

Local extinctions have cascading effects on ecosystem functions, yet little is known about the potential for the rapid evolutionary change of species in human-modified scenarios. We show that the functional extinction of large-gape seed dispersers in the Brazilian Atlantic forest is associated with the consistent reduction of the seed size of a keystone palm species. Among 22 palm populations, areas deprived of large avian frugivores for several decades present smaller seeds than nondefaunated forests, with negative consequences for palm regeneration. Coalescence and phenotypic selection models indicate that seed size reduction most likely occurred within the past 100 years, associated with human-driven fragmentation. The fast-paced defaunation of large vertebrates is most likely causing unprecedented changes in the evolutionary trajectories and community composition of tropical forests.