Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing


Autoria(s): Fusinatto, L. A.; Lopes, S.; Silva-Ferreira, A.; Alexandrino, J.; Haddad, C. F B; Rocha, C. F D; Sequeira, F.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

24/01/2013

Resumo

The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity was assessed in 21 individuals from a population (Reserva Ecológica de Guapiaçu, REGUA) locatedin the central region of the Rio de Janeiro State, in Brazil. The mean number of alleles per locus ranged from 3 to 12. Observed and expected heterozygosities ranged from 0.095 to 0.905 and from 0.094 to 0.904, respectively. After using the Bonferroni correction for multiple tests, there was no evidence of linkage disequilibrium between pairs of loci but deviations for Hardy-Weinberg equilibrium were found in 4 loci. We found no evidence for allele dropouts or stuttering, but we detected the presence of null alleles at loci Eb10 and Eb36. These markers will be useful for analyses of fine-scale population structure and determination of relative effects of habitat loss and fragmentation on population genetic variability within species. © FUNPEC-RP.

Formato

230-234

Identificador

http://dx.doi.org/10.4238/2013.January.24.15

Genetics and Molecular Research, v. 12, n. 1, p. 230-234, 2013.

1676-5680

http://hdl.handle.net/11449/74416

10.4238/2013.January.24.15

WOS:000318864400025

2-s2.0-84872892433

2-s2.0-84872892433.pdf

Idioma(s)

eng

Relação

Genetics and Molecular Research

Direitos

openAccess

Palavras-Chave #454 sequencing #Amphibians #Atlantic forest #Euparkerella brasiliensis #Microsatellites #animal tissue #Anura #Brazil #endemic species #gene isolation #gene locus #gene sequence #genetic variability #genotype #geographical variation (species) #heterozygosity #microsatellite marker #multiplex polymerase chain reaction #Neotropics #nonhuman #nucleotide sequence #null allele #process development #process optimization #pyrosequencing
Tipo

info:eu-repo/semantics/article