979 resultados para Viabilidade após a vitrificação


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Hyperthyroidism is and endocrinal dysfunction characterized by excessive serum concentrations of thyroid gland hormones, which can cause several effects on the bone tissue in humans and in animals. For a better characterization of thyrotoxicosis effects on bone metabolism, 16 cats were induced into hyperthyroid state. Results showed that, in some moments a major bone demineralization of the right distal radium estremity promoted an elevation of the serum levels of phosphorus and total alkaline phosphatase.

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Introduction: The surgery of keloids as an individual therapy with high rates of recurrence needs adjuvant therapies as complement. Recent keloids are rich in fibroblasts, which are highly radiosensitive and what explains the good results of percutaneous radiotherapy after surgery. Objective: To compare the beta-therapy results with electron-beam technique in newly operated keloids. Material and Methods: a prospective, comparative and randomized study was outlined including patients in the immediate postoperative period of surgical excision of keloids. Divided in G1 and G2 groups respectively, they received treatment with Sr90 (beta-therapy)plates or with electrons from the linear accelerator. The monitoring lasted 10 years. The results were performed using the information from patients, photograph parameters, observation and measurement of injuries, according to the criteria: Unchanged, Regular, Good and Excellent. Results: There were 26 patients, 13 in each group. In G1, 54% presented regular and unchanged improvement criteria and 46% had good or excellent criteria. In G2, the results were respectively 23% and 77%. Conclusion: The irradiation with electron-beam is better than beta-teraphy for the treatment of keloids surgery, due to the distribution in the tissue. No radio-induced tumors were observed.

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Objective: The objective of this study was evaluate if the embryos cryopreservation from OHSS patients Intracytoplasmic Sperm Injection (ICSI) cycles could be influence the clinical outcomes when compared to patients who receive oocytes from donors but the endometrium was not prepared and the embryos were cryopreserved. Methods: Fifty eight couples submitted to ICSI cycles in which 26 with OHSS clinical manifestation (OHSS group) and 32 couples who have received oocytes from donors (control group). The embryos were frozen on day+2 or +3of development. All patients included in this study had embryos crypreserved before the transfer, and in the thawing cycle, only the endometrium preparation was performed. The embryo survival, implantation, pregnancy and miscarriage rates were evaluated in the embryo thawing cycle. Results: There was no difference among the groups in relation to fertilization rate (OHSS: 71.89% ± 15.45, Control: 79.75% ± 21.68, p= 0.234), survival embryos rate (OHSS: 68.85 ± 21.10, Control: 59.53 ± 36.79, p= 0.233), high quality embryos rate (OHSS: 25.20 ± 23.90, Control: 27.40 ± 30.30, p= 0.760), implantation rate (OHSS: 17.9 ± 26.9, Control: 12.5 ± 23.7, p= 0.435), pregnancy rate (OHSS: 38.50, Control: 28.60, p= 0.441) and miscarriage rate (OHSS: 40.00, Control: 25.00, p= 0.332). Conclusion: Our findings suggest that clinical outcomes in freeze and thawing cycles were not affected by the presence of ovarian hyperstimulation syndrome clinical manifestation after controlled ovarian stimulation.

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Incluye Bibliografía

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The region of Alfenas, in the state of Minas Gerais, Brazil, is predominantly constituted of Pre-Cambrian rocks with well developed alteration profiles in association with colluvial and alluvial sediments. No study to date has examined in detail its potential use in the ceramic industry. The scarce knowledge of its mineralogical and technological properties limits its value and consequently its industrial use. Until now, these clay materials have been used in a rudimental manner, in small scale in the fabrication of red tiles. The present study aimed at analyzing these clays mineralogically (X-ray diffraction), chemically (major and minor elements by X-ray fluorescence and organic carbon analysis) and technologically (pressing granulometric distribution; mechanical resistence; water absorption, apparent porosity; linear firing shrinkage; color of firing and others) in order to better understand the raw material and develop adequate technological applications. The best results of ceramic properties were the samples with higher organic content (more plastic clays) and higher values of Al2O3 (kaolinite and gibbsite) and Fe2O3 as well lower SiO2 content and finer grain size which contribute to a better sinterization.

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This study was carried out to evaluate the effects of surface application of lime rates on soil chemical attributes and soybean yield in a no-tillage system. It was installed a field experiment in a factorial 2×4, and the factors were: two types of lime (calcitic and dolomitic); four rates (0.0 - 0.5 - 1.0 and 1.5 t ha-1 of CaCO3), in a completely randomized blocks experimental design, with four replications. The experiment was located in Matupá, Mato Grosso State, Brazil, being the work developed between November 2006 and May 2007. The evaluations were chemical attributes in the soil layers (0,0-2,5 cm, 2,5-5,0 cm and 5,0-10,0 cm), and the productive characteristics of soybean. Application of lime increased the soil pH (CaCl 2), the exchangeable Ca and Mg, the effective CEC and the base saturation only in the soil surface layer. The two limes were different only for Ca and Mg values in the first layer of soil, and only for the Mg values in the layer of 2,5-5,0 cm. The surface liming in no-tillage system did not change soybean yield.

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The growth of large cities is usually accelerated and disorganized, which causes social, economical and infrastructural conflicts and frequently, occupation in illegal areas. For a better administration of these areas, the public manager needs information about their location. This information can be obtained through land utilization and land cover maps, where orbital images of remote sensing are used as one of the most traditional sources of data. In this context, the present work tested the applicability of the object-based classification to categorize two slum areas, taking into account the structure of the streets, size of the huts, distance between the houses, among other parameters. These area combinations of physical aspects were analyzed using the image IKONOS II and the software eCognition. Slum areas tend to be, to the contrary of the planned areas, disarranged, with narrow streets, small houses built with a variety of materials and without definition of blocks. The results of land cover classification for slum areas are encouraging because they are accurate and little ambiguous in the classification process. Thus, it would allow its utilization by urban managers.

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The present study aimed to obtain information about the uterine inflammatory response (number of polymorphonuclear neutrophilic granulocytes - PMNs) in bitches after artificial insemination (AI) and identify the uterine microflora present after the following treatments: insemination using semen with extender (n=6), insemination with fresh semen (n=6) and no inseminated (n=6). The percentage of PMNs on the endometrial surface and within histological sections was evaluated together with the presence of aerobic bacteria in the uterine lumen. For endometrial cytology, there was no significative difference on the number of inflammatory cells between bitches not inseminated (3.05 ± 1.74 PMNs) and those inseminated with fresh semen (3.55 ± 1.51 PMNs); There was a significative difference in both groups compared to the inseminated with semen plus extender (7.80 ± 1.67 PMNs) (p<0.05). Histology showed that there was no significative difference on the number of inflammatory cells between bitches not inseminated (87.72 ± 35.2 PMNs) and those inseminated with fresh semen (122.97 ± 43.31 PMNs); however, it was observed differences in both groups compared to those inseminated with semen plus extender (171.94 ± 42.74 PMNs) (p<0.05). Eight animals, randomly distributed in the groups, showed the presence of Staphylococcus sp and Proteus sp., in the microbiological exam. The extender for semen, with Tris, is a potent inducer of uterine inflammation, and positive uterine cultures may be obtained during estrus without inflammation or uterine infection.

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Piptadenia moniliformis Benth. is a tree species which is important for beekeeping, as well as being recommended for soil restoration, reforestation, wood production for small civil construction projects, and cattle and sheep forage. Information on how to evaluate seed physiological quality is still scarce and in this was the study aimed to adapt the procedures of the tetrazolium test to assess the viability of P. moniliformis seeds. Four seed lots were scarified in sulphuric acid for 30 min, and soaked between paper towels at 25 °C for 24 hours. The seed coat was then removed and the naked seeds immersed in tetrazolium solutions with concentrations of 0.05, 0.075 and 0.1% for 2, 3, and 4 hours at 35 oC in the dark. Each treatment consisted of four replications of 25 seeds. The embryos were classified according to viability based on the staining patterns. The previous soaking of the seeds for 24 hours at 25 oC between paper towels, followed by the removal of the seed coat and staining of the naked seeds for 4 hours in a 0.075% tetrazolium solution at 35 oC was the most efficient method for evaluating the viability of P. moniliformis Benth seeds.

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We evaluated the effect of fertilization with 0, 50, 100 and 200 kg/ha of N, applied in each four growth intervals in the winter/spring season on dry matter yield in 30 days (DMY), leaf chlorophyll index (LCI) and total digestible nutrients (TDN), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and lignin contents, and correlation equations of LCI with DMY and CP content in the marandu and ruziziensis grasses after intercrop arrangements with corn in Red Latosol (Oxisol) under irrigation. The major DMY occurred with photoperiod increasing, however, there were different responses to this attribute over of growth intervals, between and among the grasses with the N fertilization. In irrigated croplivestock integration under savannah soil is technicality viable the cultivation of marandu and ruziziensis grasses, established by intercrop with corn at sowing or at the N fertilization. As even, in the N fertilization absence, produced satisfactory amounts of forage, with 1733 kg/ha (DMY), at the time of greatest scarcity of roughage to animals (winter/spring season). However, after the corn harvest, the N fertilization increase the DMY and improves the chemical composition, increasing the LCI, and CP and TDN contents, and decreasing NDF and ADF contents in the winter/spring season.The best correlation equations in function of LCI were logatithmica for DMY and potential for CP of the marandu grass and potential for both attributes of the ruziziensis grass.

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Intending to achieve better results on coffee production, the coffee producers began, since the 80s, to process the coffee postharvest, in order to ensure product's superior quality. However, with the processing, other problem appeared, the contamination of rivers, creeks, soil and water table from coffee wastewater (ARC). This paper had as main objective to use the ARC on the coffee plants' production verifying its viability and if the ARC is able to supply the coffee potassium requirements when they don't receive potassium chloride in the organic compound. The work was developed at Faculdade de Ciências Agronômicas - UNESP, Botucatu-SP, in a greenhouse located at Departamento de Engenharia Rural. The work consisted of 10 treatments on a 5×2 factorial (5 wastewater proportions of coffee after the harvest processing - 0%, 25%, 50%, 75% and 100% - and potassium chloride presence or absence in the composition), with 4 repetitions with a completely randomized statistical design. The ARC was applied each 48 hours in an irrigation depth of 10 mm. After 6 months, the plants' vegetative characteristic had been evaluated as well the chemical characteristics of the compound and of the plants. We observed that the treatments with potassium chloride presence showed worse vegetative characteristics compared with treatments which did not have KCl in its composition. Moreover, with the increase of the wastewater dosage, it happened a decrease in the vegetative characteristics, however an addition in the chemical characteristics of the compound. Also, the treatments without KCl presence and with 0% and 25% of coffee wastewater had been statistically equal, showing the viability in the use of the ARC in the production of coffee plants, since the compound does not contain KCl and that the water used in the composition is a mixture of 25% of ARC with 75% of common water.

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This article presents a case of relapse, with isolated neural manifestation, in a multibacillary patient previously treated with multidrug therapy for multibacillary leprosy (24 doses). The patient returned to the service six years after the end of treatment, with pain in hands and legs. He was investigated, and the serological monitoring showed an important increase in anti-phenolic glycolipid serum levels. A neural recurrence was suspected, since the patient had no new skin lesions. A new biopsy in the right ulnar nerve showed a bacilloscopy of 2 +, compatible with relapse. This is a literature review of the etiological, clinical, propedeutical and diagnostic aspects of this situation so poorly understood. © 2012 by Anais Brasileiros de Dermatologia.

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The porpoise of this study was to evaluate the effects of extracorporeal shock waves in third metacarpus bone from healthy horses by determination of bone elasticity. It were used 20 Thoroughbred horses, male and female, with two years old, on top of training and selected as the state healthy. At the beginning of the experiment (D0), all animals were submitted for evaluation of bone elasticity held in the third metacarpus bone. The animals were divided into two groups (Control Group - CG and Experimental Group - EG). The application of extracorporeal shock wave therapy (ESWT) was performed on the right forelimb of the animals in the experimental group in the same place evaluated for bone elasticity and was used apparatus for extracorporeal therapy of waves with 0.15 mJ/mm2 energy flux density and 2000 pulses with E6R20 probe, with focus feature of the shock wave of 20 mm. The ESWT were repeated every 21 days, a total of three sessions (D0, D21 and D42). The analysis of bone elasticity determination was realized at D21, D42 and D72. The average of speed ultrasound differed between groups at D21, D42 and D72, and the animals from EG had lower bone mineral density after applications of ESWT. There was also difference in the analysis of bone mass (Z-Score) between the groups at D21 and D42, which animals from EG showed a significant decrease in bone mass. The risk of fracture was higher in animals from experimental group at D21. It was concluded that ESWT is able to promote change in bone mineral density.

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Were evaluated the economic effects of four grazing heights (20, 40, 60 and 80 cm) of Tanzânia grass for beef cattle. The total area was 12 acres divided into paddocks of one hectare, with three replications. We used three animals, males, Nelore per paddock, as more animals need additional adjustment to the height you want. The reduction in sward height allowed higher stocking rate, which, even with a reduction in individual performance, there was more beef production per area. The interest rate on capital given the property represented the largest share in the final cost of production. The participation of fixed variables showed a positive linear function of the increase in height from grazing, showing significant reduction of the production scale. A reduction in cost of production per hectare per year with increasing grazing height. No differences were found in certain economic indicators, and the four systems remunerate the capital invested.

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The possibilities of using the sperm collected from the epididymis have been widely used because the fertilizing capacity sperm preservation and the possibility of using it for wild cats. But in the process of cryopreservation, some studies show a decrease in the quality of the sperm when left under cooling before frozen for some time. This study aimed to assess the quality of the epididymal sperm obtained from domestic cats after cryopreservation using a diluent based on egg yolk and glycerol (Botu-crio®), comparing the morphofunctional characteristics after cooling for 24 hours in a container of semen transport (Botu-tainer®). We use eight cats submitted to elective orchiectomy, aging from eight months, without racial determination, and good nutritional status. These sperm characteristics were: motility, vigor, concentration, membrane integrity and morphology. It has been found, after statistical analysis, that the container of semen was able to maintain sperm viability, even for 24h. We also observed a significant decrease on all parameters after frozen, consequential, probably to thermal stress that occurs in processing. However, the percentage of membrane integrity after thawing shows good employability of the Botu-crio®, which viability is possible to perform in vitro fertilization, requiring higher ratings.