Changes in leaf proline and fruit production induced by potassium stress in eggplant

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influence of physical and chemical factors during foraging and culture of the symbiont fungus in Atta sexdens rubropilosa (Hymenoptera : Formicidae)

The aim of this paper is to investigate the influence of physical and chemical factors on transport and use of substrate for Atta sexdens rubropilosa workers. Three types of rectangular fragments were used to study the physical influence factors: filter paper with paraffin, filter paper without paraffin and polyester film. To study the chemical factors, some fragments were impregnated with organic extract of orange albedo, others were soaked with soybean oil and for the remaining ones nothing was applied. The following parameters were evaluated: (i) attractiveness of substrate for transport and number of loading workers per treatment; (ii) foraged material incorporation; (iii) rejection by numbers of fragments deposited in the garbage or beside the fungus garden. All the polyester film fragments carried out to the fungus garden were subsequently rejected. We verified that chemical factors of the substrate were more quickly detected by the workers, whereas physical factors were used as a criterion in the decision-making to reject or accept the substrate collected.

Diet of invertebrates sampled in leaf-bags incubated in a tropical headwater stream

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Differentiation in Selection of Dicots and Grasses by the Leaf-Cutter Ants Atta capiguara, Atta laevigata and Atta sexdens rubropilosa

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Colonization of rocky and leaf pack substrates by benthic macroinvertebrates in a stream in southeast Brazil.

A comparative analysis was conducted of the colonization by benthic macroinvertebrates of rocky and leaf pack substrates, both natural and artificial. This colonization was evaluated by season, with the objective of ascertaining the influence of rainfall on the rate of colonization. The total density of macroinvertebrates after 21 days of colonization was significantly greater in the dry than in the wet season. When the substrate types were compared, artificial leaf pack substrate presented the smallest density for both seasons. In the wet season, Chironomidae, Leptohyphidae, Hydropsychidae, Elmidae, immature stages of Trichoptera, and Hydroptilidae showed a more representative density. In the dry season, Chironomidae, Baetidae and Oligochaeta were the most abundant taxa. The artificial rocky substrate used in this experiment was the most appropriate, due to its resemblance with natural substrate conditions in terms of the maintenance of the structural integrity of the substrate throughout the experimental period. Successional and seasonal effects were of great relevance, playing an important role in the colonization process.

Fat body in some genera of leaf-cutting ants (Hymenoptera : Formicidae). Proteins, lipids and polysaccharides detection

The comparative histochemical analysis of the fat body of workers belonging to the basal species Cyphomyrmex rimosus and Mycetarotes parallelus and to derived species Acromyrmex disciger and Atta laevigata revealed that this tissue is constituted mainly by cells denominated trophocytes and oenocytes. The trophocytes of all species studied here were characterized mainly by the proteins and lipids synthesis and storage, being the derived species the ones who have presented higher quantity of lipids in the trophocytes when compared to the trophocytes of basal species. In workers M. parallelus and A. laevigata, besides proteins and lipids, there has being observed the presence of polysaccharides, however, in C. rimosus and A. disciger these elements were detected in lower quantities. The histochemical studies of the oenocytes of basal and derived species revealed significant presence of proteins as well as lipids in these cells. In the oenocytes of derived species A. disciger and A. laevigata a higher quantity of lipidic inclusions has being observed, when compared to the basal species. (c) 2005 Elsevier Ltd. All rights reserved.

The intramandibular gland of leaf-cutting ants (Atta sexdens rubropilosa Forel 1908)

The eusociality developed in Hymenoptera and Isoptera is driven by an efficient interaction between exocrine glands and jointed appendages, both in close interaction with the environment. In this context, the mandible of ants plays an important role, since, in addition to being the main jointed appendage, it possess glandular functions. As an example we might name the two glands associated with the mandible: the mandibular and the intramandibular glands. The intramandibular gland is found inside the mandible and consists of a hypertrophied secretory epithelium and secretory cells in the mandible's lumen. The secretion of the secretory epithelium is liberated through intracuticular ducts that open at the base of hairs at the mandible's surface. The secretion of the intramandibular gland (epithelium and secretory cells) reacted positively to tests for the detection of polysaccharides and proteins, thus suggesting that it consists of glycoproteins. The ultrastructure of the secretor epithelium presents variations related to the developmental stage of the individual, showing a large number of ribosomes and microvilli close to the cuticle in young individuals, while in the older specimens it was possible to note the formation of ail intracellular reservoir. These variations of secretory epithelium, as also the interaction between the cellular groups inside the mandible, are important information about this gland in leaf-cutting ants. (c) 2005 Elsevier Ltd. All rights reserved.

Determination of the antimutagenicity of an aqueous extract of Rhizophora mangle L. (Rhizophoraceae), using in vivo and in vitro test systems

An aqueous extract of Rhizophora mangle L. bark is used as raw material in pottery making in the State of Espirito Santo, Brazil. This extract presents large quantities of tannins, compounds possessing antioxidant properties. Tannin antioxidant activity, as a plant chemical defense mechanism in the process of stabilizing free radicals, has been an incentive to studies on anti-mutagenicity. The present work aimed to evaluate possible antimutagenic activity of a R. mangle aqueous extract, using the Allium cepa test-system and micronuclear (MN) assay with blockage of cytokinesis in Chinese hamster ovary cells (CHO-K1). The Allium cepa test-system indicated antimutagenic activity against the damage induced by the mutagenic agent methyl methanesulfonate. A reduction in both MN cell frequency and chromosome breaks occurred in both the pre and post-treatment protocols. The MN testing of CHO-K1 cells revealed anti-mutagenic activity of the R. mangle extract against methyl methanesulfonate and doxorubicin in pre, simultaneous and post-treatment protocols. These results suggest the presence of phyto-constituents in the extract presenting demutagenic and bio-antimutagenic activities. Since the chemical constitution of Rhizophora mangle species presents elevated tannin content, it is highly probable that these compounds are the antimutagenic promoters themselves.

Acaricidal activity of ethanolic extract from aerial parts of Tagetes patula L. (Asteraceae) against larvae and engorged adult females of Rhipicephalus sanguineus (Latreille, 1806)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Inhibitory Action of Neem Aqueous Extract (Azadirachta indica A. Juss) on the Vitellogenesis of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) Ticks

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The functional morphology of the metapleural gland of the leaf-cutting ant Atta laevigata (Formicidae: Attini)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Secretory Profile of Metapleural Gland Cells of the Leaf-Cutting Ant Acromyrmex coronatus (Formicidae: Attini)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Yacon (Polymnia sanchifolia) extract as a substrate to produce inulinase by Kluyveromyces marxianus var. bulgaricus

The inulinase production by yeast K marxianus var. bulgaricus growing in yacon extract was investigated. The microorganism showed good development in yacon, higher enzymatic activities were achieved at 30% and 40% (v/v) of extract. The cultivation temperature (20, 25, 30, 35, 40 degreesC) neither influenced the growth or the enzymatic activity. The optimum cultivation pH was 3.5. The highest activity was observed at 60 degreesC and pH 4.0. At temperature of 55 C and 60 C occurred sharp decrease in the enzyme activity. (C) 2004 Elsevier Ltd. All rights reserved.

Leaf-cutting ant faecal fluid and mandibular gland secretion: effects on microfungi spore germination

The mandibular gland secretion (MGS) and the faecal fluid (FF) of the leaf-cutting ant Atta sexdens rubropilosa Forel affected the spore germination of selected microfungi isolated from nests of this insect. MGS was more effective than the FF, completely inhibiting the spore germination of four out of six microfungi species.

Endophytic microorganisms from coffee tissues as plant growth promoters and biocontrol agents of coffee leaf rust

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)