Acaricidal activity of ethanolic extract from aerial parts of Tagetes patula L. (Asteraceae) against larvae and engorged adult females of Rhipicephalus sanguineus (Latreille, 1806)


Autoria(s): Sanches Politi, Flavio Augusto; Figueira, Glyn Mara; Araujo, Andrea Mendez; Sampieri, Bruno Rodrigues; Camargo Mathias, Maria Izabel; Juan Szabo, Matias Pablo; Bechara, Gervasio Henrique; Santos, Lourdes Campaner dos; Vilegas, Wagner; Linhari Rodrigues Pietro, Rosemeire Cristina
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

17/12/2012

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Background: The tick Rhipicephalus sanguineus is the species with the largest worldwide distribution and is proven to be involved in the transmission of pathogens such as Babesia canis, Ehrlichia canis, Coxiella burnetii, Rickettsia ricketsii, Rickettsia conorii, among others. Studies have demonstrated acquisition of resistance to some of the active principles used in commercial formulations of acaricides. Tagetes patula (Asteraceae) is a plant with highlighted economic and commercial importance due to the production of secondary metabolites with insecticide and acaricide potential, mainly flavonoids, thiophenes and terpenes.Methods: The in vitro acaricide action of the ethanolic 70% extract from aerial parts of T. patula, obtained by percolation, was evaluated against larvae and engorged adult females of Rhipicephalus sanguineus by immersion test for 5 minutes. The chemical characterization of this extract was done by liquid chromatography coupled with mass spectrometry (LC-MS), using direct injection of sample.Results: Despite T. patula not proving lethal to adults in any of the concentrations tested, at 50.0 mg/mL oviposition rate decreased by 21.5% and eliminated 99.78% of the larvae. Also it was determined that the best results were obtained with 5 minutes of immersion. From the chromatographic analysis twelve O-glycosylated flavonoids were identified.Conclusions: This is the first report on the acaricidal activity of T. patula extract against Rh. sanguineus. If we consider the application of the product in the environment, we could completely eliminate the larval stage of development of the ixodid Rh. sanguineus.

Formato

11

Identificador

http://dx.doi.org/10.1186/1756-3305-5-295

Parasites & Vectors. London: Biomed Central Ltd., v. 5, p. 11, 2012.

1756-3305

http://hdl.handle.net/11449/19673

10.1186/1756-3305-5-295

WOS:000313219000001

WOS000313219000001.pdf

Idioma(s)

eng

Publicador

Biomed Central Ltd.

Relação

Parasites & Vectors

Direitos

openAccess

Palavras-Chave #Tagetes patula #Rhipicephalus sanguineus #flavonoids #mass spectrometry #adult immersion test #larval immersion test
Tipo

info:eu-repo/semantics/article